Restriction map for
pFN21AE2083
(Circular) (Six-base) MAPSORT of: pFN21AE2083.seq Check: 7370 from: 1 to: 6976
pFN21AE2083 6976 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3493 3493
Size: 6976
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3608 4786 278
Size: 53 83 186 936 2072 1178 2468
Fragments arranged by size:
2468 2072 1178 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3663 3834 4319 1937
Size: 1726 171 485 4594
Fragments arranged by size:
4594 1726 485 171
Acc65I G'GTAC_C
Cuts at: 4107 4297 4107
Size: 190 6786
AclI AA'CG_TT
Cuts at: 1563 5103 5476 1563
Size: 3540 373 3063
Fragments arranged by size:
3540 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2594 2602 3041 3049 5039 6423 6691 1632
Size: 962 8 439 8 1990 1384 268 1917
Fragments arranged by size:
1990 1917 1384 962 439 268 8 8
AfeI AGC'GCT
Cuts at: 2082 3761 2082
Size: 1679 5297
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6779
AflIII A'CryG_T
Cuts at: 1203 5875 6852 1203
Size: 4672 977 1327
Fragments arranged by size:
4672 1327 977
AgeI A'CCGG_T
Cuts at: 2106 5807 2106
Size: 3701 3275
AhdI GACnn_n'nnGTC
Cuts at: 5705 5705
Size: 6976
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6976
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6976
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3074 3106 3074
Size: 32 6944
AlwNI CAG_nnn'CTG
Cuts at: 2333 3899 3946 4719 6291 2333
Size: 1566 47 773 1572 3018
Fragments arranged by size:
3018 1572 1566 773 47
ApaI G_GGCC'C
Cuts at: 3386 3386
Size: 6976
ApaLI G'TGCA_C
Cuts at: 3677 5033 6189 3677
Size: 1356 1156 4464
Fragments arranged by size:
4464 1356 1156
ApoI r'AATT_y
Cuts at: 1493 2231 2306 4285 4511 4663 6772 1493
Size: 738 75 1979 226 152 2109 1697
Fragments arranged by size:
2109 1979 1697 738 226 152 75
AseI AT'TA_AT
Cuts at: 160 1032 5530 160
Size: 872 4498 1606
Fragments arranged by size:
4498 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 4146 4301 1870
Size: 73 147 2056 155 4545
Fragments arranged by size:
4545 2056 155 147 73
BamHI G'GATC_C
Cuts at: 4306 4673 4306
Size: 367 6609
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 3793 3881 4107 4297
Size: 325 217 627 451 1555 88 226 190
Cuts at: 4297 5752 618
Size: 1455 1842
Fragments arranged by size:
1842 1555 1455 627 451 325 226 217
190 88
BanII G_rGCy'C
Cuts at: 1391 3386 3912 4295 1391
Size: 1995 526 383 4072
Fragments arranged by size:
4072 1995 526 383
BbeI G_GCGC'C
Cuts at: 3885 3885
Size: 6976
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 2608 3135 3613 4005 928
Size: 971 161 548 527 478 392 3899
Fragments arranged by size:
3899 971 548 527 478 392 161
BbvCI CC'TCA_GC
Cuts at: 2066 2326 3545 2066
Size: 260 1219 5497
Fragments arranged by size:
5497 1219 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3719 3753 5167 5201 1903
Size: 34 50 34 1698 34 1414 34 3678
Fragments arranged by size:
3678 1698 1414 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4868 6078 4868
Size: 1210 5766
BclI T'GATC_A
Cuts at: 1548 3507 1548
Size: 1959 5017
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4149 4205 5587
Size: 107 122 71 631 605 2477 56 1382
Cuts at: 5587 136
Size: 1525
Fragments arranged by size:
2477 1525 1382 631 605 122 107 71
56
BglII A'GATC_T
Cuts at: 2991 3060 6971 2991
Size: 69 3911 2996
Fragments arranged by size:
3911 2996 69
BlpI GC'TnA_GC
Cuts at: 4393 4393
Size: 6976
Bme1580I G_kGCm'C
Cuts at: 1790 3386 3681 5037 6193 1790
Size: 1596 295 1356 1156 2573
Fragments arranged by size:
2573 1596 1356 1156 295
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6976
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2700 4707 5665
Size: 416 53 83 399 24 1283 2007 958
Cuts at: 5665 442
Size: 1753
Fragments arranged by size:
2007 1753 1283 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6976
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2468 2468
Size: 6976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2015 2516 2615 3176 3497 3542 5636 1239
Size: 776 501 99 561 321 45 2094 2579
Fragments arranged by size:
2579 2094 776 561 501 321 99 45
Bpu10I CC'TnA_GC
Cuts at: 2066 2326 3545 3668 2066
Size: 260 1219 123 5374
Fragments arranged by size:
5374 1219 260 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2702 5101 5966 6264 2702
Size: 2399 865 298 3414
Fragments arranged by size:
3414 2399 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5639 882
Size: 617 4140 2219
Fragments arranged by size:
4140 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4767 493
Size: 711 3563 2702
Fragments arranged by size:
3563 2702 711
BsaBI GATnn'nnATC
Cuts at: 6970 6970
Size: 6976
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3605 3882 4783 5165
Size: 53 83 186 936 2072 277 901 382
Cuts at: 5165 5800 275
Size: 635 1451
Fragments arranged by size:
2072 1451 936 901 635 382 277 186
83 53
BsaWI w'CCGG_w
Cuts at: 2106 3334 4227 5409 5807 6081 6228 6639 2106
Size: 1228 893 1182 398 274 147 411 2443
Fragments arranged by size:
2443 1228 1182 893 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2514 2544 3074 3104 1178
Size: 30 165 30 1111 30 530 30 5050
Fragments arranged by size:
5050 1111 530 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2699 2908 3281 3368 3539 3803 3992
Size: 991 522 209 373 87 171 264 189
Cuts at: 3992 4028 4267 1186
Size: 36 239 3895
Fragments arranged by size:
3895 991 522 373 264 239 209 189
171 87 36
BseYI C'CCAG_C
Cuts at: 6179 6675 6179
Size: 496 6480
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 2746 3759 1163
Size: 573 333 677 1013 4380
Fragments arranged by size:
4380 1013 677 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4246 4426 5187 6215 1540
Size: 275 186 2245 180 761 1028 2301
Fragments arranged by size:
2301 2245 1028 761 275 186 180
BsiHKAI G_wGCw'C
Cuts at: 2505 3681 3695 3912 5037 5122 6193 2505
Size: 1176 14 217 1125 85 1071 3288
Fragments arranged by size:
3288 1176 1125 1071 217 85 14
BsmI GAATG_Cn'
Cuts at: 4487 4580 4487
Size: 93 6883
BsmBI CGTCTCn'nnnn_
Cuts at: 3164 3612 3164
Size: 448 6528
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2505 3386 3681 3695 3912 4295 5037
Size: 399 715 881 295 14 217 383 742
Cuts at: 5037 5122 6193 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 881 742 715 399 383 295
217 85 14
BspEI T'CCGG_A
Cuts at: 3334 6639 3334
Size: 3305 3671
BspHI T'CATG_A
Cuts at: 4865 6595 4865
Size: 1730 5246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 2768 3444 3493 4331 844
Size: 1451 473 676 49 838 3489
Fragments arranged by size:
3489 1451 838 676 473 49
BsrBI CCG'CTC
Cuts at: 1394 1823 3529 4863 1394
Size: 429 1706 1334 3507
Fragments arranged by size:
3507 1706 1334 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5471 5645 65
Size: 1183 4223 174 1396
Fragments arranged by size:
4223 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2106 4110 4136 5620 5807 1700
Size: 110 296 2004 26 1484 187 2869
Fragments arranged by size:
2869 2004 1484 296 187 110 26
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6976
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6976
BssSI C'ACGA_G
Cuts at: 5036 6048 5036
Size: 1012 5964
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6976
BstBI TT'CG_AA
Cuts at: 5780 5780
Size: 6976
BstEII G'GTnAC_C
Cuts at: 1184 2921 1184
Size: 1737 5239
BstXI CCAn_nnnn'nTGG
Cuts at: 2639 4084 2639
Size: 1445 5531
BstYI r'GATC_y
Cuts at: 1972 2484 2991 3060 4306 4673 5058 5075 6527
Size: 512 507 69 1246 367 385 17 1452
Cuts at: 6527 6613 6625 6971 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1246 512 507 385 367 346
86 69 17 12
Bsu36I CC'TnA_GG
Cuts at: 2334 3939 2334
Size: 1605 5371
BtgI C'CryG_G
Cuts at: 513 1065 3120 3372 4153 4236 513
Size: 552 2055 252 781 83 3253
Fragments arranged by size:
3253 2055 781 552 252 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6237
Fragments arranged by size:
6237 683 56
BtsI GCAGTG_nn'
Cuts at: 2525 3747 3797 4059 4137 4492 5286 5306 2525
Size: 1222 50 262 78 355 794 20 4195
Fragments arranged by size:
4195 1222 794 355 262 78 50 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6976
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4113 4148 529
Size: 35 3549 35 3357
Fragments arranged by size:
3549 3357 35 35
DraI TTT'AAA
Cuts at: 4280 4280
Size: 6976
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5983 809
Size: 507 4667 1802
Fragments arranged by size:
4667 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3123 4134 4239
Size: 54 1425 50 275 18 1293 1011 105
Cuts at: 4239 4423 5312 6941 8
Size: 184 889 1629 43
Fragments arranged by size:
1629 1425 1293 1011 889 275 184 105
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4423 1537
Size: 275 2611 4090
Fragments arranged by size:
4090 2611 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2885 3042 3080 3119 3345 4906 1353
Size: 1532 157 38 39 226 1561 3423
Fragments arranged by size:
3423 1561 1532 226 157 39 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5563 5937 6083 387
Size: 1064 138 3974 374 146 1280
Fragments arranged by size:
3974 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3910 3910
Size: 6976
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2015 2516 2594 2602 2615 3041 3049
Size: 393 383 501 78 8 13 426 8
Cuts at: 3049 3176 3497 3542 5039 5636 6423 6691 1239
Size: 127 321 45 1497 597 787 268 1524
Fragments arranged by size:
1524 1497 787 597 501 426 393 383
321 268 127 78 45 13 8 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 3452 3966 2159
Size: 1293 514 5169
Fragments arranged by size:
5169 1293 514
EcoO109I rG'GnC_Cy
Cuts at: 2047 3383 3433 4045 2047
Size: 1336 50 612 4978
Fragments arranged by size:
4978 1336 612 50
EcoRI G'AATT_C
Cuts at: 4285 4663 4285
Size: 378 6598
EcoRV GAT'ATC
Cuts at: 2788 2788
Size: 6976
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2842 3071 2842
Size: 229 6747
FspI TGC'GCA
Cuts at: 5482 5482
Size: 6976
HaeII r_GCGC'y
Cuts at: 1678 2084 2359 3763 3885 6123 1678
Size: 406 275 1404 122 2238 2531
Fragments arranged by size:
2531 2238 1404 406 275 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3881 3913 5617 5649 5691
Size: 32 135 32 2309 32 1704 32 42
Cuts at: 5691 5723 1373
Size: 32 2626
Fragments arranged by size:
2626 2309 1704 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2344 4320 4566 669
Size: 988 281 406 1976 246 3079
Fragments arranged by size:
3079 1976 988 406 281 246
HindIII A'AGCT_T
Cuts at: 748 2678 2912 748
Size: 1930 234 4812
Fragments arranged by size:
4812 1930 234
HpaI GTT'AAC
Cuts at: 4566 4566
Size: 6976
KasI G'GCGC_C
Cuts at: 3881 3881
Size: 6976
KpnI G_GTAC'C
Cuts at: 4111 4301 4111
Size: 190 6786
MfeI C'AATT_G
Cuts at: 4575 4575
Size: 6976
MluI A'CGCG_T
Cuts at: 6852 6852
Size: 6976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6090 6274 1305
Size: 4785 184 2007
Fragments arranged by size:
4785 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5154
Fragments arranged by size:
5154 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 4934 5293 5452 518
Size: 648 1201 2567 359 159 2042
Fragments arranged by size:
2567 2042 1201 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3021 3374 3553 3754 4392 5069 6217 6462
Size: 1415 353 179 201 638 677 1148 245
Cuts at: 6462 1606
Size: 2120
Fragments arranged by size:
2120 1415 1148 677 638 353 245 201
179
NaeI GCC'GGC
Cuts at: 4138 4138
Size: 6976
NarI GG'CG_CC
Cuts at: 3882 3882
Size: 6976
NcoI C'CATG_G
Cuts at: 513 1065 4153 4236 513
Size: 552 3088 83 3253
Fragments arranged by size:
3253 3088 552 83
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6976
NgoMIV G'CCGG_C
Cuts at: 4136 4136
Size: 6976
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6976
NotI GC'GGCC_GC
Cuts at: 4423 4423
Size: 6976
NspI r_CATG'y
Cuts at: 1132 1229 2350 2395 2716 4334 5879 1132
Size: 97 1121 45 321 1618 1545 2229
Fragments arranged by size:
2229 1618 1545 1121 321 97 45
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6976
PciI A'CATG_T
Cuts at: 5875 5875
Size: 6976
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2772 3449 1108
Size: 1664 677 4635
Fragments arranged by size:
4635 1664 677
PfoI T'CCnGG_A
Cuts at: 2487 2721 2948 3096 3519 2487
Size: 234 227 148 423 5944
Fragments arranged by size:
5944 423 234 227 148
PmeI GTTT'AAAC
Cuts at: 4280 4280
Size: 6976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3074 3106 5045 5077 6558 6590 3074
Size: 32 1939 32 1481 32 3460
Fragments arranged by size:
3460 1939 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2047 3433 4045 2047
Size: 1386 612 4978
Fragments arranged by size:
4978 1386 612
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6976
PsiI TTA'TAA
Cuts at: 4546 4546
Size: 6976
PspOMI G'GGCC_C
Cuts at: 3382 3382
Size: 6976
PstI C_TGCA'G
Cuts at: 830 3081 3147 3804 4054 4328 830
Size: 2251 66 657 250 274 3478
Fragments arranged by size:
3478 2251 657 274 250 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6976
PvuII CAG'CTG
Cuts at: 3021 3553 3021
Size: 532 6444
SacI G_AGCT'C
Cuts at: 3912 3912
Size: 6976
SacII CC_GC'GG
Cuts at: 3375 3375
Size: 6976
SalI G'TCGA_C
Cuts at: 1936 4318 1936
Size: 2382 4594
SbfI CC_TGCA'GG
Cuts at: 4328 4328
Size: 6976
ScaI AGT'ACT
Cuts at: 3482 5224 6936 3482
Size: 1742 1712 3522
Fragments arranged by size:
3522 1742 1712
SexAI A'CCwGG_T
Cuts at: 3873 3873
Size: 6976
SfcI C'TryA_G
Cuts at: 826 1045 2264 2575 3077 3143 3800 4050 4324
Size: 219 1219 311 502 66 657 250 274
Cuts at: 4324 5459 6140 6331 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1219 1135 681 657 502 311 274
250 219 191 66
SfoI GGC'GCC
Cuts at: 3883 3883
Size: 6976
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6976
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6976
SmaI CCC'GGG
Cuts at: 1872 4303 1872
Size: 2431 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2681 5080 5981 6243 820
Size: 197 926 738 2399 901 262 1553
Fragments arranged by size:
2399 1553 926 901 738 262 197
SnaBI TAC'GTA
Cuts at: 493 4767 493
Size: 4274 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6976
SphI G_CATG'C
Cuts at: 1132 4334 1132
Size: 3202 3774
SspI AAT'ATT
Cuts at: 5 52 4900 5
Size: 47 4848 2081
Fragments arranged by size:
4848 2081 47
StuI AGG'CCT
Cuts at: 1517 3152 1517
Size: 1635 5341
StyI C'CwwG_G
Cuts at: 513 1065 3448 4153 4236 4415 513
Size: 552 2383 705 83 179 3074
Fragments arranged by size:
3074 2383 705 552 179 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2054 5003 5020 5173 5358 5803 325
Size: 1255 474 2949 17 153 185 445 1498
Fragments arranged by size:
2949 1498 1255 474 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3480 3771 5222
Size: 274 80 33 51 1443 1503 291 1451
Cuts at: 5222 6934 96
Size: 1712 138
Fragments arranged by size:
1712 1503 1451 1443 291 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2393 2411 2425 2443 2514 2546 3341 3373 6936
Size: 18 14 18 71 32 795 32 3563
Cuts at: 6936 6968 2393
Size: 32 2401
Fragments arranged by size:
3563 2401 795 71 32 32 32 18
18 14
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6976
XbaI T'CTAG_A
Cuts at: 4312 4312
Size: 6976
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3397 3397
Size: 6976
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6976
XmaI C'CCGG_G
Cuts at: 1870 4301 1870
Size: 2431 4545
XmnI GAAnn'nnTTC
Cuts at: 3089 5105 3089
Size: 2016 4960
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3606 4784 276
Size: 53 83 186 936 2072 1178 2468
Fragments arranged by size:
2468 2072 1178 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII FseI
FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII
SanDI SapI SfiI SrfI SwaI