Restriction map for
pFN21AE2078
(Circular) (Six-base) MAPSORT of: pFN21AE2078.seq Check: 8325 from: 1 to: 6949
pFN21AE2078 6949 bp
With 182 enzymes: SgfI *
May 14, 2013 11:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4759 278
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4292 1937
Size: 2355 4594
Acc65I G'GTAC_C
Cuts at: 4270 4270
Size: 6949
AclI AA'CG_TT
Cuts at: 1563 5076 5449 1563
Size: 3513 373 3063
Fragments arranged by size:
3513 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2634 2997 3844 5012 6396 6664 1632
Size: 1002 363 847 1168 1384 268 1917
Fragments arranged by size:
1917 1384 1168 1002 847 363 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6752
AflIII A'CryG_T
Cuts at: 1203 3746 4246 5848 6825 1203
Size: 2543 500 1602 977 1327
Fragments arranged by size:
2543 1602 1327 977 500
AgeI A'CCGG_T
Cuts at: 5780 5780
Size: 6949
AhdI GACnn_n'nnGTC
Cuts at: 5678 5678
Size: 6949
AleI CACnn'nnGTG
Cuts at: 1166 2145 3685 3861 1166
Size: 979 1540 176 4254
Fragments arranged by size:
4254 1540 979 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6949
AlwNI CAG_nnn'CTG
Cuts at: 3641 3839 4692 6264 3641
Size: 198 853 1572 4326
Fragments arranged by size:
4326 1572 853 198
ApaLI G'TGCA_C
Cuts at: 3199 5006 6162 3199
Size: 1807 1156 3986
Fragments arranged by size:
3986 1807 1156
ApoI r'AATT_y
Cuts at: 1493 2258 2914 3220 3296 4258 4484 4636 6745
Size: 765 656 306 76 962 226 152 2109
Cuts at: 6745 1493
Size: 1697
Fragments arranged by size:
2109 1697 962 765 656 306 226 152
76
AseI AT'TA_AT
Cuts at: 160 1032 3693 5503 160
Size: 872 2661 1810 1606
Fragments arranged by size:
2661 1810 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
AvaI C'yCGr_G
Cuts at: 1870 1943 4215 4274 1870
Size: 73 2272 59 4545
Fragments arranged by size:
4545 2272 73 59
BamHI G'GATC_C
Cuts at: 3173 3890 4279 4646 3173
Size: 717 389 367 5476
Fragments arranged by size:
5476 717 389 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4270 5725 618
Size: 325 217 627 2483 1455 1842
Fragments arranged by size:
2483 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4002 4268 1391
Size: 2611 266 4072
Fragments arranged by size:
4072 2611 266
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2178 2215 2947 928
Size: 971 279 37 732 4930
Fragments arranged by size:
4930 971 732 279 37
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5140 5174 1903
Size: 34 3203 34 3678
Fragments arranged by size:
3678 3203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4841 6051 4841
Size: 1210 5739
BclI T'GATC_A
Cuts at: 1548 2689 4068 1548
Size: 1141 1379 4429
Fragments arranged by size:
4429 1379 1141
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5560 136
Size: 107 122 71 631 605 3888 1525
Fragments arranged by size:
3888 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6944 6944
Size: 6949
BlpI GC'TnA_GC
Cuts at: 4366 4366
Size: 6949
Bme1580I G_kGCm'C
Cuts at: 1790 3203 5010 6166 1790
Size: 1413 1807 1156 2573
Fragments arranged by size:
2573 1807 1413 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6949
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4680 5638 442
Size: 416 53 83 399 24 3263 958 1753
Fragments arranged by size:
3263 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6949
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4194 4194
Size: 6949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3045 5609 1239
Size: 1806 2564 2579
Fragments arranged by size:
2579 2564 1806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2580 3356 5074 5939 6237 2580
Size: 776 1718 865 298 3292
Fragments arranged by size:
3292 1718 865 776 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3117 4168 5612 882
Size: 617 1618 1051 1444 2219
Fragments arranged by size:
2219 1618 1444 1051 617
BsaAI yAC'GTr
Cuts at: 493 1204 3747 4740 493
Size: 711 2543 993 2702
Fragments arranged by size:
2702 2543 993 711
BsaBI GATnn'nnATC
Cuts at: 3465 6943 3465
Size: 3478 3471
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4756 5138 5773 275
Size: 53 83 186 936 3223 382 635 1451
Fragments arranged by size:
3223 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2792 5382 5780 6054 6201 6612 2792
Size: 2590 398 274 147 411 3129
Fragments arranged by size:
3129 2590 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3731 3761 1178
Size: 30 165 30 2328 30 4366
Fragments arranged by size:
4366 2328 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 3752 4128 4183 4186 1186
Size: 838 1728 376 55 3 3949
Fragments arranged by size:
3949 1728 838 376 55 3
BseYI C'CCAG_C
Cuts at: 3634 4200 6152 6648 3634
Size: 566 1952 496 3935
Fragments arranged by size:
3935 1952 566 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2544 3532 3726 3863 3958 1163
Size: 573 808 988 194 137 95 4154
Fragments arranged by size:
4154 988 808 573 194 137 95
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4399 5160 6188 1540
Size: 275 186 2398 761 1028 2301
Fragments arranged by size:
2398 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3010 3203 4002 5010 5095 6166 3010
Size: 193 799 1008 85 1071 3793
Fragments arranged by size:
3793 1071 1008 799 193 85
BsmI GAATG_Cn'
Cuts at: 3455 3551 4460 4553 3455
Size: 96 909 93 5851
Fragments arranged by size:
5851 909 96 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4237 4237
Size: 6949
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3010 3203 4002 4268 5010 5095 6166
Size: 399 1220 193 799 266 742 85 1071
Cuts at: 6166 1391
Size: 2174
Fragments arranged by size:
2174 1220 1071 799 742 399 266 193
85
BspEI T'CCGG_A
Cuts at: 2792 6612 2792
Size: 3820 3129
BspHI T'CATG_A
Cuts at: 2473 2929 4838 6568 2473
Size: 456 1909 1730 2854
Fragments arranged by size:
2854 1909 1730 456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3698 4304 844
Size: 2854 606 3489
Fragments arranged by size:
3489 2854 606
BsrBI CCG'CTC
Cuts at: 1394 1823 4836 1394
Size: 429 3013 3507
Fragments arranged by size:
3507 3013 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3707 4011 5444 5618 65
Size: 1183 2459 304 1433 174 1396
Fragments arranged by size:
2459 1433 1396 1183 304 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5593 5780 1700
Size: 110 3783 187 2869
Fragments arranged by size:
3783 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3536 96
Size: 3440 3509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6949
BssSI C'ACGA_G
Cuts at: 2394 2718 5009 6021 2394
Size: 324 2291 1012 3322
Fragments arranged by size:
3322 2291 1012 324
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3548 794
Size: 2754 4195
BstBI TT'CG_AA
Cuts at: 5753 5753
Size: 6949
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6949
BstXI CCAn_nnnn'nTGG
Cuts at: 2204 2396 2567 2204
Size: 192 171 6586
Fragments arranged by size:
6586 192 171
BstYI r'GATC_y
Cuts at: 1972 3173 3890 4279 4646 5031 5048 6500 6586
Size: 1201 717 389 367 385 17 1452 86
Cuts at: 6586 6598 6944 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1201 717 389 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2511 2511
Size: 6949
BtgI C'CryG_G
Cuts at: 513 1065 2004 2203 2350 513
Size: 552 939 199 147 5112
Fragments arranged by size:
5112 939 552 199 147
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6210
Fragments arranged by size:
6210 683 56
BtsI GCAGTG_nn'
Cuts at: 3851 4108 4465 5259 5279 3851
Size: 257 357 794 20 5521
Fragments arranged by size:
5521 794 357 257 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6914
DraI TTT'AAA
Cuts at: 2079 2263 2704 4164 4253 2079
Size: 184 441 1460 89 4775
Fragments arranged by size:
4775 1460 441 184 89
DraIII CAC_nnn'GTG
Cuts at: 3548 3548
Size: 6949
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5956 809
Size: 507 4640 1802
Fragments arranged by size:
4640 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3370 4396 5285
Size: 54 1425 50 275 18 1540 1026 889
Cuts at: 5285 6914 8
Size: 1629 43
Fragments arranged by size:
1629 1540 1425 1026 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4396 1537
Size: 275 2584 4090
Fragments arranged by size:
4090 2584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 3338 4879 1353
Size: 872 1113 1541 3423
Fragments arranged by size:
3423 1541 1113 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5536 5910 6056 387
Size: 1064 138 3947 374 146 1280
Fragments arranged by size:
3947 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4000 4000
Size: 6949
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2634 2997 3045 3844 5012 5609 6396
Size: 393 1002 363 48 799 1168 597 787
Cuts at: 6396 6664 1239
Size: 268 1524
Fragments arranged by size:
1524 1168 1002 799 787 597 393 363
268 48
EcoO109I rG'GnC_Cy
Cuts at: 2857 3527 2857
Size: 670 6279
EcoRI G'AATT_C
Cuts at: 2914 4258 4636 2914
Size: 1344 378 5227
Fragments arranged by size:
5227 1344 378
EcoRV GAT'ATC
Cuts at: 2742 2742
Size: 6949
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 6949
FspI TGC'GCA
Cuts at: 5455 5455
Size: 6949
HaeII r_GCGC'y
Cuts at: 1678 6096 1678
Size: 4418 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2044 2076 2731 2763 4015
Size: 32 135 32 472 32 655 32 1252
Cuts at: 4015 4047 5590 5622 5664 5696 1373
Size: 32 1543 32 42 32 2626
Fragments arranged by size:
2626 1543 1252 655 472 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4293 4539 669
Size: 988 281 2355 246 3079
Fragments arranged by size:
3079 2355 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6949
HpaI GTT'AAC
Cuts at: 4539 4539
Size: 6949
KpnI G_GTAC'C
Cuts at: 4274 4274
Size: 6949
MfeI C'AATT_G
Cuts at: 4548 4548
Size: 6949
MluI A'CGCG_T
Cuts at: 4246 6825 4246
Size: 2579 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2983 3139 6063 6247 1305
Size: 1678 156 2924 184 2007
Fragments arranged by size:
2924 2007 1678 184 156
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3372 10
Size: 54 1425 343 1540 3587
Fragments arranged by size:
3587 1540 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2145 2202 2325 2478 3685 3861 4076
Size: 648 979 57 123 153 1207 176 215
Cuts at: 4076 4907 5266 5425 518
Size: 831 359 159 2042
Fragments arranged by size:
2042 1207 979 831 648 359 215 176
159 153 123 57
MspA1I CmG'CkG
Cuts at: 1606 2654 4210 4365 5042 6190 6435 1606
Size: 1048 1556 155 677 1148 245 2120
Fragments arranged by size:
2120 1556 1148 1048 677 245 155
NcoI C'CATG_G
Cuts at: 513 1065 2004 2203 2350 513
Size: 552 939 199 147 5112
Fragments arranged by size:
5112 939 552 199 147
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6949
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6949
NotI GC'GGCC_GC
Cuts at: 4396 4396
Size: 6949
NspI r_CATG'y
Cuts at: 1132 1229 2609 4307 5852 1132
Size: 97 1380 1698 1545 2229
Fragments arranged by size:
2229 1698 1545 1380 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6949
PciI A'CATG_T
Cuts at: 5848 5848
Size: 6949
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2888 1108
Size: 1780 5169
PmeI GTTT'AAAC
Cuts at: 4253 4253
Size: 6949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5018 5050 6531 6563 5018
Size: 32 1481 32 5404
Fragments arranged by size:
5404 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2857 3527 2857
Size: 670 6279
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6949
PsiI TTA'TAA
Cuts at: 4519 4519
Size: 6949
PstI C_TGCA'G
Cuts at: 830 4301 830
Size: 3471 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6949
PvuII CAG'CTG
Cuts at: 2654 2654
Size: 6949
SacI G_AGCT'C
Cuts at: 4002 4002
Size: 6949
SalI G'TCGA_C
Cuts at: 1936 4291 1936
Size: 2355 4594
SbfI CC_TGCA'GG
Cuts at: 4301 4301
Size: 6949
ScaI AGT'ACT
Cuts at: 2286 5197 6909 2286
Size: 2911 1712 2326
Fragments arranged by size:
2911 2326 1712
SfcI C'TryA_G
Cuts at: 826 1045 3307 3427 4297 5432 6113 6304 826
Size: 219 2262 120 870 1135 681 191 1471
Fragments arranged by size:
2262 1471 1135 870 681 219 191 120
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6949
SmaI CCC'GGG
Cuts at: 1872 4276 1872
Size: 2404 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2595 3335 5053 5954 6216 820
Size: 197 926 652 740 1718 901 262 1553
Fragments arranged by size:
1718 1553 926 901 740 652 262 197
SnaBI TAC'GTA
Cuts at: 493 4740 493
Size: 4247 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6949
SphI G_CATG'C
Cuts at: 1132 4307 1132
Size: 3175 3774
SspI AAT'ATT
Cuts at: 5 52 2708 2887 4873 5
Size: 47 2656 179 1986 2081
Fragments arranged by size:
2656 2081 1986 179 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6949
StyI C'CwwG_G
Cuts at: 513 1065 2004 2203 2350 4388 513
Size: 552 939 199 147 2038 3074
Fragments arranged by size:
3074 2038 939 552 199 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2457 2979 4976 4993 5146 5331 5776
Size: 1255 877 522 1997 17 153 185 445
Cuts at: 5776 325
Size: 1498
Fragments arranged by size:
1997 1498 1255 877 522 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2284 3536 3830
Size: 274 80 33 51 1443 307 1252 294
Cuts at: 3830 5195 6907 96
Size: 1365 1712 138
Fragments arranged by size:
1712 1443 1365 1252 307 294 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3731 3763 6909 6941 3731
Size: 32 3146 32 3739
Fragments arranged by size:
3739 3146 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6949
XbaI T'CTAG_A
Cuts at: 2188 4285 2188
Size: 2097 4852
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2396 3380 2396
Size: 984 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6949
XmaI C'CCGG_G
Cuts at: 1870 4274 1870
Size: 2404 4545
XmnI GAAnn'nnTTC
Cuts at: 5078 5078
Size: 6949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4757 276
Size: 53 83 186 936 3223 2468
Fragments arranged by size:
3223 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI