Restriction map for
pFN21AE2077
(Circular) (Six-base) MAPSORT of: pFN21AE2077.seq Check: 7510 from: 1 to: 6940
pFN21AE2077 6940 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3294 4750 278
Size: 53 83 186 936 1758 1456 2468
Fragments arranged by size:
2468 1758 1456 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 4283 1937
Size: 1497 849 4594
Fragments arranged by size:
4594 1497 849
Acc65I G'GTAC_C
Cuts at: 4261 4261
Size: 6940
AclI AA'CG_TT
Cuts at: 1563 3328 5067 5440 1563
Size: 1765 1739 373 3063
Fragments arranged by size:
3063 1765 1739 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 2453 3221 3384 3785 5003 6387 6655
Size: 725 96 768 163 401 1218 1384 268
Cuts at: 6655 1632
Size: 1917
Fragments arranged by size:
1917 1384 1218 768 725 401 268 163
96
AfeI AGC'GCT
Cuts at: 3454 3454
Size: 6940
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6743
AflIII A'CryG_T
Cuts at: 1203 5839 6816 1203
Size: 4636 977 1327
Fragments arranged by size:
4636 1327 977
AgeI A'CCGG_T
Cuts at: 5771 5771
Size: 6940
AhdI GACnn_n'nnGTC
Cuts at: 5669 5669
Size: 6940
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6940
AlwNI CAG_nnn'CTG
Cuts at: 2947 4683 6255 2947
Size: 1736 1572 3632
Fragments arranged by size:
3632 1736 1572
ApaLI G'TGCA_C
Cuts at: 4997 6153 4997
Size: 1156 5784
ApoI r'AATT_y
Cuts at: 1493 2594 2619 2677 2745 3050 3953 4249 4475
Size: 1101 25 58 68 305 903 296 226
Cuts at: 4475 4627 6736 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1101 903 305 296 226 152
68 58 25
AseI AT'TA_AT
Cuts at: 160 1032 5494 160
Size: 872 4462 1606
Fragments arranged by size:
4462 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
AvaI C'yCGr_G
Cuts at: 1870 1943 4265 1870
Size: 73 2322 4545
Fragments arranged by size:
4545 2322 73
BamHI G'GATC_C
Cuts at: 4270 4637 4270
Size: 367 6573
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4261 5716 618
Size: 325 217 627 2474 1455 1842
Fragments arranged by size:
2474 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4259 1391
Size: 2868 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2532 3246 928
Size: 971 633 714 4622
Fragments arranged by size:
4622 971 714 633
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5131 5165 1903
Size: 34 3194 34 3678
Fragments arranged by size:
3678 3194 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4832 6042 4832
Size: 1210 5730
BclI T'GATC_A
Cuts at: 1548 3085 3375 1548
Size: 1537 290 5113
Fragments arranged by size:
5113 1537 290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5551 136
Size: 107 122 71 631 605 3879 1525
Fragments arranged by size:
3879 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6935 6935
Size: 6940
BlpI GC'TnA_GC
Cuts at: 4357 4357
Size: 6940
Bme1580I G_kGCm'C
Cuts at: 1790 5001 6157 1790
Size: 3211 1156 2573
Fragments arranged by size:
3211 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6940
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2284 2329 4137
Size: 416 53 83 399 24 867 45 1808
Cuts at: 4137 4671 5629 442
Size: 534 958 1753
Fragments arranged by size:
1808 1753 958 867 534 416 399 83
53 45 24
BmtI G_CTAG'C
Cuts at: 1056 2432 1056
Size: 1376 5564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5600 1239
Size: 4361 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5065 5930 6228 5065
Size: 865 298 5777
Fragments arranged by size:
5777 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5603 882
Size: 617 4104 2219
Fragments arranged by size:
4104 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4731 493
Size: 711 3527 2702
Fragments arranged by size:
3527 2702 711
BsaBI GATnn'nnATC
Cuts at: 6934 6934
Size: 6940
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3291 4211 4747 5129
Size: 53 83 186 936 1758 920 536 382
Cuts at: 5129 5764 275
Size: 635 1451
Fragments arranged by size:
1758 1451 936 920 635 536 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4029 5373 5771 6045 6192 6603 4029
Size: 1344 398 274 147 411 4366
Fragments arranged by size:
4366 1344 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3553 3583 1178
Size: 30 165 30 2150 30 4535
Fragments arranged by size:
4535 2150 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6940
BseYI C'CCAG_C
Cuts at: 6143 6639 6143
Size: 496 6444
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2316 4035 1163
Size: 573 580 1719 4068
Fragments arranged by size:
4068 1719 580 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4390 5151 6179 1540
Size: 275 186 2389 761 1028 2301
Fragments arranged by size:
2389 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5001 5086 6157 5001
Size: 85 1071 5784
Fragments arranged by size:
5784 1071 85
BsmI GAATG_Cn'
Cuts at: 2493 3321 4451 4544 2493
Size: 828 1130 93 4889
Fragments arranged by size:
4889 1130 828 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4259 5001 5086 6157 1391
Size: 399 2469 742 85 1071 2174
Fragments arranged by size:
2469 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6603 6603
Size: 6940
BspHI T'CATG_A
Cuts at: 2794 4065 4829 6559 2794
Size: 1271 764 1730 3175
Fragments arranged by size:
3175 1730 1271 764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4295 844
Size: 3451 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4827 1394
Size: 429 3004 3507
Fragments arranged by size:
3507 3004 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5435 5609 65
Size: 1183 4187 174 1396
Fragments arranged by size:
4187 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5584 5771 1700
Size: 110 3774 187 2869
Fragments arranged by size:
3774 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3613 3683 96
Size: 3517 70 3353
Fragments arranged by size:
3517 3353 70
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6940
BssSI C'ACGA_G
Cuts at: 3250 5000 6012 3250
Size: 1750 1012 4178
Fragments arranged by size:
4178 1750 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6940
BstBI TT'CG_AA
Cuts at: 5744 5744
Size: 6940
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6940
BstXI CCAn_nnnn'nTGG
Cuts at: 3355 3355
Size: 6940
BstYI r'GATC_y
Cuts at: 1972 4270 4637 5022 5039 6491 6577 6589 6935
Size: 2298 367 385 17 1452 86 12 346
Cuts at: 6935 1972
Size: 1977
Fragments arranged by size:
2298 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3435 3435
Size: 6940
BtgI C'CryG_G
Cuts at: 513 1065 2073 2548 3729 513
Size: 552 1008 475 1181 3724
Fragments arranged by size:
3724 1181 1008 552 475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6201
Fragments arranged by size:
6201 683 56
BtsI GCAGTG_nn'
Cuts at: 4456 5250 5270 4456
Size: 794 20 6126
Fragments arranged by size:
6126 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6940
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6905
DraI TTT'AAA
Cuts at: 3958 4244 3958
Size: 286 6654
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3290 5947 809
Size: 507 1974 2657 1802
Fragments arranged by size:
2657 1974 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4387 5276 6905
Size: 54 1425 50 275 18 2557 889 1629
Cuts at: 6905 8
Size: 43
Fragments arranged by size:
2557 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4387 1537
Size: 275 2575 4090
Fragments arranged by size:
4090 2575 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4870 1353
Size: 3517 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5527 5901 6047 387
Size: 1064 138 3938 374 146 1280
Fragments arranged by size:
3938 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2453 3221 3384 3785 5003 5600
Size: 393 725 96 768 163 401 1218 597
Cuts at: 5600 6387 6655 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1218 787 768 725 597 401 393
268 163 96
EcoNI CCTnn'n_nnAGG
Cuts at: 2996 2996
Size: 6940
EcoO109I rG'GnC_Cy
Cuts at: 2971 3115 2971
Size: 144 6796
EcoRI G'AATT_C
Cuts at: 4249 4627 4249
Size: 378 6562
EcoRV GAT'ATC
Cuts at: 2128 2128
Size: 6940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2387 3310 2387
Size: 923 6017
FspI TGC'GCA
Cuts at: 5446 5446
Size: 6940
HaeII r_GCGC'y
Cuts at: 1678 3456 6087 1678
Size: 1778 2631 2531
Fragments arranged by size:
2631 2531 1778
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2105 2137 3893 3925 5581
Size: 32 135 32 533 32 1756 32 1656
Cuts at: 5581 5613 5655 5687 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1756 1656 533 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2804 4284 4530 669
Size: 988 281 866 1480 246 3079
Fragments arranged by size:
3079 1480 988 866 281 246
HindIII A'AGCT_T
Cuts at: 748 2670 748
Size: 1922 5018
HpaI GTT'AAC
Cuts at: 4530 4530
Size: 6940
KpnI G_GTAC'C
Cuts at: 4265 4265
Size: 6940
MfeI C'AATT_G
Cuts at: 4539 4539
Size: 6940
MluI A'CGCG_T
Cuts at: 6816 6816
Size: 6940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2064 3087 6054 6238 1305
Size: 759 1023 2967 184 2007
Fragments arranged by size:
2967 2007 1023 759 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5118
Fragments arranged by size:
5118 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2961 3263 3353 3648 3815 4898 5257
Size: 648 1795 302 90 295 167 1083 359
Cuts at: 5257 5416 518
Size: 159 2042
Fragments arranged by size:
2042 1795 1083 648 359 302 295 167
159 90
MspA1I CmG'CkG
Cuts at: 1606 4356 5033 6181 6426 1606
Size: 2750 677 1148 245 2120
Fragments arranged by size:
2750 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2548 3729 513
Size: 552 1483 1181 3724
Fragments arranged by size:
3724 1483 1181 552
NdeI CA'TA_TG
Cuts at: 387 2934 3721 387
Size: 2547 787 3606
Fragments arranged by size:
3606 2547 787
NheI G'CTAG_C
Cuts at: 1052 2428 1052
Size: 1376 5564
NotI GC'GGCC_GC
Cuts at: 4387 4387
Size: 6940
NspI r_CATG'y
Cuts at: 1132 1229 2843 3010 4298 5843 1132
Size: 97 1614 167 1288 1545 2229
Fragments arranged by size:
2229 1614 1545 1288 167 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6940
PciI A'CATG_T
Cuts at: 5839 5839
Size: 6940
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6940
PmeI GTTT'AAAC
Cuts at: 4244 4244
Size: 6940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5009 5041 6522 6554 5009
Size: 32 1481 32 5395
Fragments arranged by size:
5395 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6940
PsiI TTA'TAA
Cuts at: 3792 4510 3792
Size: 718 6222
PstI C_TGCA'G
Cuts at: 830 4292 830
Size: 3462 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6940
SalI G'TCGA_C
Cuts at: 1936 4282 1936
Size: 2346 4594
SbfI CC_TGCA'GG
Cuts at: 4292 4292
Size: 6940
ScaI AGT'ACT
Cuts at: 2253 3185 5188 6900 2253
Size: 932 2003 1712 2293
Fragments arranged by size:
2293 2003 1712 932
SfcI C'TryA_G
Cuts at: 826 1045 3539 4053 4288 5423 6104 6295 826
Size: 219 2494 514 235 1135 681 191 1471
Fragments arranged by size:
2494 1471 1135 681 514 235 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6940
SmaI CCC'GGG
Cuts at: 1872 4267 1872
Size: 2395 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5044 5945 6207 820
Size: 197 926 3101 901 262 1553
Fragments arranged by size:
3101 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4731 493
Size: 4238 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6940
SphI G_CATG'C
Cuts at: 1132 4298 1132
Size: 3166 3774
SspI AAT'ATT
Cuts at: 5 52 4864 5
Size: 47 4812 2081
Fragments arranged by size:
4812 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6940
StyI C'CwwG_G
Cuts at: 513 1065 2161 2548 3729 4379 513
Size: 552 1096 387 1181 650 3074
Fragments arranged by size:
3074 1181 1096 650 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2348 4967 4984 5137 5322 5767 325
Size: 1255 768 2619 17 153 185 445 1498
Fragments arranged by size:
2619 1498 1255 768 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2251 3183 3613
Size: 274 80 33 51 1443 274 932 430
Cuts at: 3613 3683 5186 6898 96
Size: 70 1503 1712 138
Fragments arranged by size:
1712 1503 1443 932 430 274 274 138
80 70 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6900 6932 6900
Size: 32 6908
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6940
XbaI T'CTAG_A
Cuts at: 4276 4276
Size: 6940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3647 3647
Size: 6940
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6940
XmaI C'CCGG_G
Cuts at: 1870 4265 1870
Size: 2395 4545
XmnI GAAnn'nnTTC
Cuts at: 2466 2594 2718 2745 5069 2466
Size: 128 124 27 2324 4337
Fragments arranged by size:
4337 2324 128 124 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3292 4748 276
Size: 53 83 186 936 1758 1456 2468
Fragments arranged by size:
2468 1758 1456 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI