Restriction map for
pFN21AE2076
(Circular) (Six-base) MAPSORT of: pFN21AE2076.seq Check: 6444 from: 1 to: 6940
pFN21AE2076 6940 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 4750 278
Size: 53 83 186 936 2125 1089 2468
Fragments arranged by size:
2468 2125 1089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4283 1937
Size: 2346 4594
Acc65I G'GTAC_C
Cuts at: 4261 4261
Size: 6940
AclI AA'CG_TT
Cuts at: 1563 5067 5440 1563
Size: 3504 373 3063
Fragments arranged by size:
3504 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2444 3309 3498 3714 4047 4104 5003
Size: 633 179 865 189 216 333 57 899
Cuts at: 5003 6387 6655 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 899 865 633 333 268 216
189 179 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6743
AflIII A'CryG_T
Cuts at: 1203 5839 6816 1203
Size: 4636 977 1327
Fragments arranged by size:
4636 1327 977
AgeI A'CCGG_T
Cuts at: 5771 5771
Size: 6940
AhdI GACnn_n'nnGTC
Cuts at: 5669 5669
Size: 6940
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6940
AlwNI CAG_nnn'CTG
Cuts at: 2566 2606 4683 6255 2566
Size: 40 2077 1572 3251
Fragments arranged by size:
3251 2077 1572 40
ApaI G_GGCC'C
Cuts at: 2046 2046
Size: 6940
ApaLI G'TGCA_C
Cuts at: 3296 3776 4997 6153 3296
Size: 480 1221 1156 4083
Fragments arranged by size:
4083 1221 1156 480
ApoI r'AATT_y
Cuts at: 1493 2929 3081 4141 4249 4475 4627 6736 1493
Size: 1436 152 1060 108 226 152 2109 1697
Fragments arranged by size:
2109 1697 1436 1060 226 152 152 108
AseI AT'TA_AT
Cuts at: 160 1032 2912 5494 160
Size: 872 1880 2582 1606
Fragments arranged by size:
2582 1880 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 4265 1870
Size: 73 74 2248 4545
Fragments arranged by size:
4545 2248 74 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2546 2579 2546
Size: 33 6907
BamHI G'GATC_C
Cuts at: 2716 3091 4007 4270 4637 2716
Size: 375 916 263 367 5019
Fragments arranged by size:
5019 916 375 367 263
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 2178 4261 5716 618
Size: 325 217 627 282 109 2083 1455 1842
Fragments arranged by size:
2083 1842 1455 627 325 282 217 109
BanII G_rGCy'C
Cuts at: 1391 2046 2580 3647 4259 1391
Size: 655 534 1067 612 4072
Fragments arranged by size:
4072 1067 655 612 534
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2996 3327 3359 3661 928
Size: 971 1097 331 32 302 4207
Fragments arranged by size:
4207 1097 971 331 302 32
BbvCI CC'TCA_GC
Cuts at: 2856 3570 2856
Size: 714 6226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5131 5165 1903
Size: 34 3194 34 3678
Fragments arranged by size:
3678 3194 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4832 6042 3581
Size: 1251 1210 4479
Fragments arranged by size:
4479 1251 1210
BclI T'GATC_A
Cuts at: 1548 2521 4075 1548
Size: 973 1554 4413
Fragments arranged by size:
4413 1554 973
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2020 5551 136
Size: 107 122 71 631 605 348 3531 1525
Fragments arranged by size:
3531 1525 631 605 348 122 107 71
BglII A'GATC_T
Cuts at: 2308 2434 6935 2308
Size: 126 4501 2313
Fragments arranged by size:
4501 2313 126
BlpI GC'TnA_GC
Cuts at: 4357 4357
Size: 6940
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2072 3300 3780 5001 6157 1790
Size: 256 26 1228 480 1221 1156 2573
Fragments arranged by size:
2573 1228 1221 1156 480 256 26
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6940
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4671 5629 442
Size: 416 53 83 399 24 3254 958 1753
Fragments arranged by size:
3254 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6940
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2327 2327
Size: 6940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2322 3614 5600 1239
Size: 1083 1292 1986 2579
Fragments arranged by size:
2579 1986 1292 1083
Bpu10I CC'TnA_GC
Cuts at: 2856 3570 2856
Size: 714 6226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3042 3791 4089 5065 5930 6228 3042
Size: 749 298 976 865 298 3754
Fragments arranged by size:
3754 976 865 749 298 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5603 882
Size: 617 4104 2219
Fragments arranged by size:
4104 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4731 493
Size: 711 3527 2702
Fragments arranged by size:
3527 2702 711
BsaBI GATnn'nnATC
Cuts at: 2520 6934 2520
Size: 4414 2526
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3658 4747 5129 5764
Size: 53 83 186 936 2125 1089 382 635
Cuts at: 5764 275
Size: 1451
Fragments arranged by size:
2125 1451 1089 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5373 5771 6045 6192 6603 5373
Size: 398 274 147 411 5710
Fragments arranged by size:
5710 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2859 2889 1178
Size: 30 165 30 1456 30 5229
Fragments arranged by size:
5229 1456 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2319 2880 4092 1186
Size: 1133 561 1212 4034
Fragments arranged by size:
4034 1212 1133 561
BseYI C'CCAG_C
Cuts at: 2065 6143 6639 2065
Size: 4078 496 2366
Fragments arranged by size:
4078 2366 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3806 4044 1163
Size: 573 2070 238 4059
Fragments arranged by size:
4059 2070 573 238
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2030 4390 5151 6179 1540
Size: 275 186 29 2360 761 1028 2301
Fragments arranged by size:
2360 2301 1028 761 275 186 29
BsiHKAI G_wGCw'C
Cuts at: 2580 3300 3780 3807 5001 5086 6157 2580
Size: 720 480 27 1194 85 1071 3363
Fragments arranged by size:
3363 1194 1071 720 480 85 27
BsmI GAATG_Cn'
Cuts at: 3462 4451 4544 3462
Size: 989 93 5858
Fragments arranged by size:
5858 989 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2072 2580 3300 3647 3780 3807
Size: 399 256 26 508 720 347 133 27
Cuts at: 3807 4259 5001 5086 6157 1391
Size: 452 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 720 508 452 399 347
256 133 85 27 26
BspEI T'CCGG_A
Cuts at: 6603 6603
Size: 6940
BspHI T'CATG_A
Cuts at: 2266 4177 4829 6559 2266
Size: 1911 652 1730 2647
Fragments arranged by size:
2647 1911 1730 652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4295 844
Size: 3451 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2062 4827 1394
Size: 429 188 51 2765 3507
Fragments arranged by size:
3507 2765 429 188 51
BsrDI GCAATG_nn'
Cuts at: 65 1248 2292 3363 5435 5609 65
Size: 1183 1044 1071 2072 174 1396
Fragments arranged by size:
2072 1396 1183 1071 1044 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 5584 5771 1700
Size: 110 262 3512 187 2869
Fragments arranged by size:
3512 2869 262 187 110
BsrGI T'GTAC_A
Cuts at: 96 2537 3235 3977 96
Size: 2441 698 742 3059
Fragments arranged by size:
3059 2441 742 698
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6940
BssSI C'ACGA_G
Cuts at: 5000 6012 5000
Size: 1012 5928
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2677 3784 794
Size: 1883 1107 3950
Fragments arranged by size:
3950 1883 1107
BstBI TT'CG_AA
Cuts at: 5744 5744
Size: 6940
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6940
BstXI CCAn_nnnn'nTGG
Cuts at: 2691 2691
Size: 6940
BstYI r'GATC_y
Cuts at: 1972 2308 2434 2716 3091 4007 4270 4637 5022
Size: 336 126 282 375 916 263 367 385
Cuts at: 5022 5039 6491 6577 6589 6935 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 916 385 375 367 346 336
282 263 126 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3873 3873
Size: 6940
BtgI C'CryG_G
Cuts at: 513 1065 2004 2631 2817 513
Size: 552 939 627 186 4636
Fragments arranged by size:
4636 939 627 552 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6201
Fragments arranged by size:
6201 683 56
BtsI GCAGTG_nn'
Cuts at: 2555 3300 4456 5250 5270 2555
Size: 745 1156 794 20 4225
Fragments arranged by size:
4225 1156 794 745 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6940
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6905
DraI TTT'AAA
Cuts at: 4244 4244
Size: 6940
DraIII CAC_nnn'GTG
Cuts at: 3304 3784 3304
Size: 480 6460
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3917 5947 809
Size: 507 2601 2030 1802
Fragments arranged by size:
2601 2030 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 4156 4387
Size: 54 1425 50 275 18 197 2129 231
Cuts at: 4387 5276 6905 8
Size: 889 1629 43
Fragments arranged by size:
2129 1629 1425 889 275 231 197 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2027 4387 1537
Size: 275 215 2360 4090
Fragments arranged by size:
4090 2360 275 215
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 2736 3635 4870 1353
Size: 930 453 899 1235 3423
Fragments arranged by size:
3423 1235 930 899 453
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5527 5901 6047 387
Size: 1064 138 3938 374 146 1280
Fragments arranged by size:
3938 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2578 2578
Size: 6940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 2322 2444 3309 3498 3614 3714
Size: 393 633 57 122 865 189 116 100
Cuts at: 3714 4047 4104 5003 5600 6387 6655 1239
Size: 333 57 899 597 787 268 1524
Fragments arranged by size:
1524 899 865 787 633 597 393 333
268 189 122 116 100 57 57
EcoO109I rG'GnC_Cy
Cuts at: 2043 2851 2870 2043
Size: 808 19 6113
Fragments arranged by size:
6113 808 19
EcoRI G'AATT_C
Cuts at: 4249 4627 4249
Size: 378 6562
EcoRV GAT'ATC
Cuts at: 3510 3510
Size: 6940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3083 3083
Size: 6940
FspI TGC'GCA
Cuts at: 5446 5446
Size: 6940
HaeII r_GCGC'y
Cuts at: 1678 6087 1678
Size: 4409 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 3664 3696 3847
Size: 32 135 32 803 32 1257 32 151
Cuts at: 3847 3879 5581 5613 5655 5687 1373
Size: 32 1702 32 42 32 2626
Fragments arranged by size:
2626 1702 1257 803 151 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 4233 4284 4530 669
Size: 988 281 940 1355 51 246 3079
Fragments arranged by size:
3079 1355 988 940 281 246 51
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6940
HpaI GTT'AAC
Cuts at: 4233 4530 4233
Size: 297 6643
KpnI G_GTAC'C
Cuts at: 4265 4265
Size: 6940
MfeI C'AATT_G
Cuts at: 4539 4539
Size: 6940
MluI A'CGCG_T
Cuts at: 6816 6816
Size: 6940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6054 6238 1305
Size: 4749 184 2007
Fragments arranged by size:
4749 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4158 10
Size: 54 1425 343 2326 2792
Fragments arranged by size:
2792 2326 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 3758 3926 4045 4898 5257 5416
Size: 648 1051 1541 168 119 853 359 159
Cuts at: 5416 518
Size: 2042
Fragments arranged by size:
2042 1541 1051 853 648 359 168 159
119
MspA1I CmG'CkG
Cuts at: 1606 3271 4356 5033 6181 6426 1606
Size: 1665 1085 677 1148 245 2120
Fragments arranged by size:
2120 1665 1148 1085 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2631 2817 513
Size: 552 939 627 186 4636
Fragments arranged by size:
4636 939 627 552 186
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6940
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6940
NotI GC'GGCC_GC
Cuts at: 4387 4387
Size: 6940
NspI r_CATG'y
Cuts at: 1132 1229 2739 3620 4298 5843 1132
Size: 97 1510 881 678 1545 2229
Fragments arranged by size:
2229 1545 1510 881 678 97
PasI CC'CwG_GG
Cuts at: 1348 2046 1348
Size: 698 6242
PciI A'CATG_T
Cuts at: 5839 5839
Size: 6940
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6940
PmeI GTTT'AAAC
Cuts at: 4244 4244
Size: 6940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2103 2135 5009 5041 6522 6554 2103
Size: 32 2874 32 1481 32 2489
Fragments arranged by size:
2874 2489 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2851 2870 2851
Size: 19 6921
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6940
PsiI TTA'TAA
Cuts at: 4510 4510
Size: 6940
PspOMI G'GGCC_C
Cuts at: 2042 2042
Size: 6940
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3970 4002 3970
Size: 32 6908
PstI C_TGCA'G
Cuts at: 830 2562 2682 4292 830
Size: 1732 120 1610 3478
Fragments arranged by size:
3478 1732 1610 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6940
PvuII CAG'CTG
Cuts at: 3271 3271
Size: 6940
SacI G_AGCT'C
Cuts at: 2580 2580
Size: 6940
SalI G'TCGA_C
Cuts at: 1936 4282 1936
Size: 2346 4594
SanDI GG'GwC_CC
Cuts at: 2851 2851
Size: 6940
SapI GCTCTTCn'nnn_
Cuts at: 3635 3635
Size: 6940
SbfI CC_TGCA'GG
Cuts at: 4292 4292
Size: 6940
ScaI AGT'ACT
Cuts at: 5188 6900 5188
Size: 1712 5228
SfcI C'TryA_G
Cuts at: 826 1045 2558 2678 2831 3014 3325 3635 3742
Size: 219 1513 120 153 183 311 310 107
Cuts at: 3742 4288 5423 6104 6295 826
Size: 546 1135 681 191 1471
Fragments arranged by size:
1513 1471 1135 681 546 311 310 219
191 183 153 120 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6940
SgrAI Cr'CCGG_yG
Cuts at: 1700 2072 1700
Size: 372 6568
SmaI CCC'GGG
Cuts at: 1872 2019 4267 1872
Size: 147 2248 4545
Fragments arranged by size:
4545 2248 147
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3057 3806 4104 5044 5945 6207
Size: 197 926 1114 749 298 940 901 262
Cuts at: 6207 820
Size: 1553
Fragments arranged by size:
1553 1114 940 926 901 749 298 262
197
SnaBI TAC'GTA
Cuts at: 493 4731 493
Size: 4238 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6940
SphI G_CATG'C
Cuts at: 1132 3620 4298 1132
Size: 2488 678 3774
Fragments arranged by size:
3774 2488 678
SspI AAT'ATT
Cuts at: 5 52 2257 4864 5
Size: 47 2205 2607 2081
Fragments arranged by size:
2607 2205 2081 47
StuI AGG'CCT
Cuts at: 1517 2231 2662 1517
Size: 714 431 5795
Fragments arranged by size:
5795 714 431
StyI C'CwwG_G
Cuts at: 513 1065 2004 2631 2817 4153 4379 513
Size: 552 939 627 186 1336 226 3074
Fragments arranged by size:
3074 1336 939 627 552 226 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4967 4984 5137 5322 5767 325
Size: 1255 3387 17 153 185 445 1498
Fragments arranged by size:
3387 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2537 2941 3235
Size: 274 80 33 51 1443 560 404 294
Cuts at: 3235 3977 5186 6898 96
Size: 742 1209 1712 138
Fragments arranged by size:
1712 1443 1209 742 560 404 294 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2708 2740 6900 6932 2708
Size: 32 4160 32 2716
Fragments arranged by size:
4160 2716 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6940
XbaI T'CTAG_A
Cuts at: 3121 4015 4276 3121
Size: 894 261 5785
Fragments arranged by size:
5785 894 261
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6940
XmaI C'CCGG_G
Cuts at: 1870 2017 4265 1870
Size: 147 2248 4545
Fragments arranged by size:
4545 2248 147
XmnI GAAnn'nnTTC
Cuts at: 3003 5069 3003
Size: 2066 4874
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 4748 276
Size: 53 83 186 936 2125 1089 2468
Fragments arranged by size:
2468 2125 1089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII
SacII SexAI SfiI SfoI SrfI SwaI XcmI