Restriction map for
pFN21AE2073
(Circular) (Six-base) MAPSORT of: pFN21AE2073.seq Check: 5503 from: 1 to: 6913
pFN21AE2073 6913 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4723 278
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 4256 1937
Size: 786 1533 4594
Fragments arranged by size:
4594 1533 786
Acc65I G'GTAC_C
Cuts at: 4234 4234
Size: 6913
AclI AA'CG_TT
Cuts at: 1563 5040 5413 1563
Size: 3477 373 3063
Fragments arranged by size:
3477 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2702 3851 4976 6360 6628 1632
Size: 1070 1149 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1149 1125 1070 268
AflII C'TTAA_G
Cuts at: 820 1017 4046 820
Size: 197 3029 3687
Fragments arranged by size:
3687 3029 197
AflIII A'CryG_T
Cuts at: 1203 3451 5812 6789 1203
Size: 2248 2361 977 1327
Fragments arranged by size:
2361 2248 1327 977
AgeI A'CCGG_T
Cuts at: 5744 5744
Size: 6913
AhdI GACnn_n'nnGTC
Cuts at: 5642 5642
Size: 6913
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6913
AlwNI CAG_nnn'CTG
Cuts at: 3644 4656 6228 3644
Size: 1012 1572 4329
Fragments arranged by size:
4329 1572 1012
ApaLI G'TGCA_C
Cuts at: 2637 4970 6126 2637
Size: 2333 1156 3424
Fragments arranged by size:
3424 2333 1156
ApoI r'AATT_y
Cuts at: 1493 2106 2291 3897 4207 4222 4448 4600 6709
Size: 613 185 1606 310 15 226 152 2109
Cuts at: 6709 1493
Size: 1697
Fragments arranged by size:
2109 1697 1606 613 310 226 185 152
15
AseI AT'TA_AT
Cuts at: 160 1032 5467 160
Size: 872 4435 1606
Fragments arranged by size:
4435 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 4238 1870
Size: 73 136 2159 4545
Fragments arranged by size:
4545 2159 136 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2242 2275 2242
Size: 33 6880
BamHI G'GATC_C
Cuts at: 3496 4243 4610 3496
Size: 747 367 5799
Fragments arranged by size:
5799 747 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 3784 4234 5689 618
Size: 325 217 627 1184 813 450 1455 1842
Fragments arranged by size:
1842 1455 1184 813 627 450 325 217
BanII G_rGCy'C
Cuts at: 1391 2776 4232 1391
Size: 1385 1456 4072
Fragments arranged by size:
4072 1456 1385
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2850 2915 3579 928
Size: 971 951 65 664 4262
Fragments arranged by size:
4262 971 951 664 65
BbvCI CC'TCA_GC
Cuts at: 3413 3516 3413
Size: 103 6810
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3437 3471 5104 5138 1903
Size: 34 1500 34 1633 34 3678
Fragments arranged by size:
3678 1633 1500 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4805 6015 4805
Size: 1210 5703
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6913
BfrBI ATG'CAT
Cuts at: 2033 2987 3859 2033
Size: 954 872 5087
Fragments arranged by size:
5087 954 872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5524 136
Size: 107 122 71 631 605 3852 1525
Fragments arranged by size:
3852 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2713 3173 6908 2713
Size: 460 3735 2718
Fragments arranged by size:
3735 2718 460
BlpI GC'TnA_GC
Cuts at: 3651 4330 3651
Size: 679 6234
Bme1580I G_kGCm'C
Cuts at: 1790 2641 2974 4974 6130 1790
Size: 851 333 2000 1156 2573
Fragments arranged by size:
2573 2000 1156 851 333
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6913
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2977 3223 4644
Size: 416 53 83 399 24 1560 246 1421
Cuts at: 4644 5602 442
Size: 958 1753
Fragments arranged by size:
1753 1560 1421 958 416 399 246 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2760 3114 5573 1239
Size: 1521 354 2459 2579
Fragments arranged by size:
2579 2459 1521 354
Bpu10I CC'TnA_GC
Cuts at: 3413 3516 3413
Size: 103 6810
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2573 3351 5038 5903 6201 2573
Size: 778 1687 865 298 3285
Fragments arranged by size:
3285 1687 865 778 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4157 5576 882
Size: 617 2658 1419 2219
Fragments arranged by size:
2658 2219 1419 617
BsaAI yAC'GTr
Cuts at: 493 1204 2218 4704 493
Size: 711 1014 2486 2702
Fragments arranged by size:
2702 2486 1014 711
BsaBI GATnn'nnATC
Cuts at: 6907 6907
Size: 6913
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4720 5102 5737 275
Size: 53 83 186 936 3187 382 635 1451
Fragments arranged by size:
3187 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5346 5744 6018 6165 6576 5346
Size: 398 274 147 411 5683
Fragments arranged by size:
5683 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3108 3138 3993 4023 1178
Size: 30 165 30 1705 30 855 30 4068
Fragments arranged by size:
4068 1705 855 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2538 3066 3111 4001 1186
Size: 1352 528 45 890 4098
Fragments arranged by size:
4098 1352 890 528 45
BseYI C'CCAG_C
Cuts at: 3891 3953 6116 6612 3891
Size: 62 2163 496 4192
Fragments arranged by size:
4192 2163 496 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3356 1163
Size: 573 1620 4720
Fragments arranged by size:
4720 1620 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4363 5124 6152 1540
Size: 275 186 2362 761 1028 2301
Fragments arranged by size:
2362 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2641 2776 4974 5059 6130 2641
Size: 135 2198 85 1071 3424
Fragments arranged by size:
3424 2198 1071 135 85
BsmI GAATG_Cn'
Cuts at: 3360 4424 4517 3360
Size: 1064 93 5756
Fragments arranged by size:
5756 1064 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3365 3365
Size: 6913
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2641 2776 2974 4232 4974 5059 6130
Size: 399 851 135 198 1258 742 85 1071
Cuts at: 6130 1391
Size: 2174
Fragments arranged by size:
2174 1258 1071 851 742 399 198 135
85
BspEI T'CCGG_A
Cuts at: 6576 6576
Size: 6913
BspHI T'CATG_A
Cuts at: 3811 4802 6532 3811
Size: 991 1730 4192
Fragments arranged by size:
4192 1730 991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4268 844
Size: 3424 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2557 4800 1394
Size: 429 734 2243 3507
Fragments arranged by size:
3507 2243 734 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5408 5582 65
Size: 1183 4160 174 1396
Fragments arranged by size:
4160 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5557 5744 1700
Size: 110 3747 187 2869
Fragments arranged by size:
3747 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6913
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6913
BssSI C'ACGA_G
Cuts at: 4154 4973 5985 4154
Size: 819 1012 5082
Fragments arranged by size:
5082 1012 819
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2039 794
Size: 1245 5668
BstBI TT'CG_AA
Cuts at: 3271 5717 3271
Size: 2446 4467
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6913
BstYI r'GATC_y
Cuts at: 1972 2713 3173 3496 4098 4243 4610 4995 5012
Size: 741 460 323 602 145 367 385 17
Cuts at: 5012 6464 6550 6562 6908 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 741 602 460 385 367 346
323 145 86 17 12
BstZ17I GTA'TAC
Cuts at: 2724 2724
Size: 6913
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5422
Fragments arranged by size:
5422 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6174
Fragments arranged by size:
6174 683 56
BtsI GCAGTG_nn'
Cuts at: 2839 4429 5223 5243 2839
Size: 1590 794 20 4509
Fragments arranged by size:
4509 1590 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6913
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6878
DraI TTT'AAA
Cuts at: 3057 4217 3057
Size: 1160 5753
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5920 809
Size: 507 4604 1802
Fragments arranged by size:
4604 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3464 4360 5249
Size: 54 1425 50 275 18 1634 896 889
Cuts at: 5249 6878 8
Size: 1629 43
Fragments arranged by size:
1634 1629 1425 896 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4360 1537
Size: 275 2548 4090
Fragments arranged by size:
4090 2548 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3084 3526 4843 1353
Size: 1731 442 1317 3423
Fragments arranged by size:
3423 1731 1317 442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5500 5874 6020 387
Size: 1064 138 3911 374 146 1280
Fragments arranged by size:
3911 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2774 2774
Size: 6913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2702 2760 3114 3851 4976 5573 6360
Size: 393 1070 58 354 737 1125 597 787
Cuts at: 6360 6628 1239
Size: 268 1524
Fragments arranged by size:
1524 1125 1070 787 737 597 393 354
268 58
EcoRI G'AATT_C
Cuts at: 4222 4600 4222
Size: 378 6535
FspI TGC'GCA
Cuts at: 5419 5419
Size: 6913
HaeII r_GCGC'y
Cuts at: 1678 2892 6060 1678
Size: 1214 3168 2531
Fragments arranged by size:
3168 2531 1214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2928 2960 3993 4025 5554
Size: 32 135 32 1356 32 1033 32 1529
Cuts at: 5554 5586 5628 5660 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1529 1356 1033 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2947 4257 4503 669
Size: 988 281 1009 1310 246 3079
Fragments arranged by size:
3079 1310 1009 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6913
HpaI GTT'AAC
Cuts at: 4503 4503
Size: 6913
KpnI G_GTAC'C
Cuts at: 4238 4238
Size: 6913
MfeI C'AATT_G
Cuts at: 4070 4512 4070
Size: 442 6471
MluI A'CGCG_T
Cuts at: 6789 6789
Size: 6913
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2563 6027 6211 1305
Size: 1258 3464 184 2007
Fragments arranged by size:
3464 2007 1258 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3466 10
Size: 54 1425 343 1634 3457
Fragments arranged by size:
3457 1634 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3456 3974 4871 5230 5389 518
Size: 648 2290 518 897 359 159 2042
Fragments arranged by size:
2290 2042 897 648 518 359 159
MspA1I CmG'CkG
Cuts at: 1606 4329 5006 6154 6399 1606
Size: 2723 677 1148 245 2120
Fragments arranged by size:
2723 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5422
Fragments arranged by size:
5422 939 552
NdeI CA'TA_TG
Cuts at: 387 2035 2101 2587 2625 3259 387
Size: 1648 66 486 38 634 4041
Fragments arranged by size:
4041 1648 634 486 66 38
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6913
NotI GC'GGCC_GC
Cuts at: 4360 4360
Size: 6913
NsiI A_TGCA'T
Cuts at: 2035 2989 3861 2035
Size: 954 872 5087
Fragments arranged by size:
5087 954 872
NspI r_CATG'y
Cuts at: 1132 1229 2991 3107 3455 3877 4271 5816 1132
Size: 97 1762 116 348 422 394 1545 2229
Fragments arranged by size:
2229 1762 1545 422 394 348 116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6913
PciI A'CATG_T
Cuts at: 3451 5812 3451
Size: 2361 4552
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2762 4108 1108
Size: 1654 1346 3913
Fragments arranged by size:
3913 1654 1346
PmeI GTTT'AAAC
Cuts at: 4217 4217
Size: 6913
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4982 5014 6495 6527 4982
Size: 32 1481 32 5368
Fragments arranged by size:
5368 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6913
PsiI TTA'TAA
Cuts at: 4483 4483
Size: 6913
PstI C_TGCA'G
Cuts at: 830 4265 830
Size: 3435 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6913
SacI G_AGCT'C
Cuts at: 2776 2776
Size: 6913
SalI G'TCGA_C
Cuts at: 1936 4255 1936
Size: 2319 4594
SbfI CC_TGCA'GG
Cuts at: 4265 4265
Size: 6913
ScaI AGT'ACT
Cuts at: 2443 5161 6873 2443
Size: 2718 1712 2483
Fragments arranged by size:
2718 2483 1712
SfcI C'TryA_G
Cuts at: 826 1045 2428 2632 4261 5396 6077 6268 826
Size: 219 1383 204 1629 1135 681 191 1471
Fragments arranged by size:
1629 1471 1383 1135 681 219 204 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6913
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6913
SmaI CCC'GGG
Cuts at: 1872 4240 1872
Size: 2368 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 3366 4046 5017 5918 6180
Size: 197 926 609 814 680 971 901 262
Cuts at: 6180 820
Size: 1553
Fragments arranged by size:
1553 971 926 901 814 680 609 262
197
SnaBI TAC'GTA
Cuts at: 493 2218 4704 493
Size: 1725 2486 2702
Fragments arranged by size:
2702 2486 1725
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6913
SphI G_CATG'C
Cuts at: 1132 2991 4271 1132
Size: 1859 1280 3774
Fragments arranged by size:
3774 1859 1280
SspI AAT'ATT
Cuts at: 5 52 3187 3383 4837 5
Size: 47 3135 196 1454 2081
Fragments arranged by size:
3135 2081 1454 196 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6913
StyI C'CwwG_G
Cuts at: 513 1065 2004 2481 3549 4352 513
Size: 552 939 477 1068 803 3074
Fragments arranged by size:
3074 1068 939 803 552 477
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2989 3150 4940 4957 5110 5295 5740
Size: 1255 1409 161 1790 17 153 185 445
Cuts at: 5740 325
Size: 1498
Fragments arranged by size:
1790 1498 1409 1255 445 185 161 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2278 2441 3115
Size: 274 80 33 51 1443 301 163 674
Cuts at: 3115 3830 4132 5159 6871 96
Size: 715 302 1027 1712 138
Fragments arranged by size:
1712 1443 1027 715 674 302 301 274
163 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2843 2875 6873 6905 2843
Size: 32 3998 32 2851
Fragments arranged by size:
3998 2851 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6913
XbaI T'CTAG_A
Cuts at: 4249 4249
Size: 6913
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6913
XmaI C'CCGG_G
Cuts at: 1870 4238 1870
Size: 2368 4545
XmnI GAAnn'nnTTC
Cuts at: 3267 5042 3267
Size: 1775 5138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4721 276
Size: 53 83 186 936 3187 2468
Fragments arranged by size:
3187 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BstXI Bsu36I DraIII EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI