Restriction map for
pFN21AE2070
(Circular) (Six-base) MAPSORT of: pFN21AE2070.seq Check: 4056 from: 1 to: 6889
pFN21AE2070 6889 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3305 3305
Size: 6889
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4699 278
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2645 3099 3558 4104 4232 1937
Size: 708 454 459 546 128 4594
Fragments arranged by size:
4594 708 546 459 454 128
Acc65I G'GTAC_C
Cuts at: 4210 4210
Size: 6889
AclI AA'CG_TT
Cuts at: 1563 5016 5389 1563
Size: 3453 373 3063
Fragments arranged by size:
3453 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 3032 3074 3762 4092 4952 6336 6604
Size: 408 992 42 688 330 860 1384 268
Cuts at: 6604 1632
Size: 1917
Fragments arranged by size:
1917 1384 992 860 688 408 330 268
42
AfeI AGC'GCT
Cuts at: 4034 4034
Size: 6889
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6692
AflIII A'CryG_T
Cuts at: 1203 3942 3978 5788 6765 1203
Size: 2739 36 1810 977 1327
Fragments arranged by size:
2739 1810 1327 977 36
AgeI A'CCGG_T
Cuts at: 5720 5720
Size: 6889
AhdI GACnn_n'nnGTC
Cuts at: 5618 5618
Size: 6889
AleI CACnn'nnGTG
Cuts at: 1166 3524 1166
Size: 2358 4531
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6889
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3929 3961 3929
Size: 32 6857
AlwNI CAG_nnn'CTG
Cuts at: 2263 2544 3400 4632 6204 2263
Size: 281 856 1232 1572 2948
Fragments arranged by size:
2948 1572 1232 856 281
ApaI G_GGCC'C
Cuts at: 3460 3460
Size: 6889
ApaLI G'TGCA_C
Cuts at: 4946 6102 4946
Size: 1156 5733
ApoI r'AATT_y
Cuts at: 1493 4198 4424 4576 6685 1493
Size: 2705 226 152 2109 1697
Fragments arranged by size:
2705 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5443 160
Size: 872 4411 1606
Fragments arranged by size:
4411 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
AvaI C'yCGr_G
Cuts at: 1870 1943 2854 3615 3916 4214 1870
Size: 73 911 761 301 298 4545
Fragments arranged by size:
4545 911 761 301 298 73
BamHI G'GATC_C
Cuts at: 3477 4219 4586 3477
Size: 742 367 5780
Fragments arranged by size:
5780 742 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2722 3877 4210 5665 618
Size: 325 217 627 935 1155 333 1455 1842
Fragments arranged by size:
1842 1455 1155 935 627 333 325 217
BanII G_rGCy'C
Cuts at: 1391 2633 2946 3112 3204 3378 3460 4176 4208
Size: 1242 313 166 92 174 82 716 32
Cuts at: 4208 1391
Size: 4072
Fragments arranged by size:
4072 1242 716 313 174 166 92 82
32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2538 2572 5080 5114 1903
Size: 34 601 34 2508 34 3678
Fragments arranged by size:
3678 2508 601 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4781 5991 4781
Size: 1210 5679
BclI T'GATC_A
Cuts at: 1548 2592 3135 1548
Size: 1044 543 5302
Fragments arranged by size:
5302 1044 543
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2514 5500 136
Size: 107 122 71 631 605 842 2986 1525
Fragments arranged by size:
2986 1525 842 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6884 6884
Size: 6889
BlpI GC'TnA_GC
Cuts at: 3372 3607 4306 3372
Size: 235 699 5955
Fragments arranged by size:
5955 699 235
Bme1580I G_kGCm'C
Cuts at: 1790 2727 2909 3460 3696 4950 6106 1790
Size: 937 182 551 236 1254 1156 2573
Fragments arranged by size:
2573 1254 1156 937 551 236 182
BmgBI CAC'GTC
Cuts at: 1318 3066 1318
Size: 1748 5141
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2121 2595 4141
Size: 416 53 83 399 24 704 474 1546
Cuts at: 4141 4620 5578 442
Size: 479 958 1753
Fragments arranged by size:
1753 1546 958 704 479 474 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6889
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3931 3641
Size: 290 6599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2678 2893 5549 1239
Size: 1439 215 2656 2579
Fragments arranged by size:
2656 2579 1439 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3755 4045 5014 5879 6177 3755
Size: 290 969 865 298 4467
Fragments arranged by size:
4467 969 865 298 290
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2279 4120 5552 882
Size: 617 780 1841 1432 2219
Fragments arranged by size:
2219 1841 1432 780 617
BsaAI yAC'GTr
Cuts at: 493 1204 3981 4126 4680 493
Size: 711 2777 145 554 2702
Fragments arranged by size:
2777 2702 711 554 145
BsaBI GATnn'nnATC
Cuts at: 6883 6883
Size: 6889
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4696 5078 5713 275
Size: 53 83 186 936 3163 382 635 1451
Fragments arranged by size:
3163 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2358 5322 5720 5994 6141 6552 2358
Size: 2964 398 274 147 411 2695
Fragments arranged by size:
2964 2695 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2486 2516 3929 3959 1178
Size: 30 165 30 1083 30 1413 30 4108
Fragments arranged by size:
4108 1413 1083 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2393 2465 2675 1186
Size: 1207 72 210 5400
Fragments arranged by size:
5400 1207 210 72
BseYI C'CCAG_C
Cuts at: 3507 3763 3780 4035 6092 6588 3507
Size: 256 17 255 2057 496 3808
Fragments arranged by size:
3808 2057 496 256 255 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2578 3695 1163
Size: 573 842 1117 4357
Fragments arranged by size:
4357 1117 842 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4339 5100 6128 1540
Size: 275 186 2338 761 1028 2301
Fragments arranged by size:
2338 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2633 2982 3627 4950 5035 6106 2633
Size: 349 645 1323 85 1071 3416
Fragments arranged by size:
3416 1323 1071 645 349 85
BsmI GAATG_Cn'
Cuts at: 2642 4082 4400 4493 2642
Size: 1440 318 93 5038
Fragments arranged by size:
5038 1440 318 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2633 2727 2909 2946 2982 3112 3204
Size: 399 843 94 182 37 36 130 92
Cuts at: 3204 3378 3460 3627 3696 4176 4208 4950 5035
Size: 174 82 167 69 480 32 742 85
Cuts at: 5035 6106 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 843 742 480 399 182 174
167 130 94 92 85 82 69 37
36 32
BspEI T'CCGG_A
Cuts at: 6552 6552
Size: 6889
BspHI T'CATG_A
Cuts at: 4778 6508 4778
Size: 1730 5159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2552 2688 3305 4081 4244 844
Size: 1708 136 617 776 163 3489
Fragments arranged by size:
3489 1708 776 617 163 136
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 3915 4776 1394
Size: 429 1504 588 861 3507
Fragments arranged by size:
3507 1504 861 588 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5384 5558 65
Size: 1183 4136 174 1396
Fragments arranged by size:
4136 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3776 5533 5720 1700
Size: 110 1966 1757 187 2869
Fragments arranged by size:
2869 1966 1757 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6889
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6889
BssSI C'ACGA_G
Cuts at: 4949 5961 4949
Size: 1012 5877
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3577 794
Size: 2783 4106
BstBI TT'CG_AA
Cuts at: 5693 5693
Size: 6889
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6889
BstXI CCAn_nnnn'nTGG
Cuts at: 2105 2656 4126 2105
Size: 551 1470 4868
Fragments arranged by size:
4868 1470 551
BstYI r'GATC_y
Cuts at: 1972 2094 3477 4219 4586 4971 4988 6440 6526
Size: 122 1383 742 367 385 17 1452 86
Cuts at: 6526 6538 6884 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1383 742 385 367 346 122
86 17 12
BstZ17I GTA'TAC
Cuts at: 2646 2646
Size: 6889
Bsu36I CC'TnA_GG
Cuts at: 2718 2718
Size: 6889
BtgI C'CryG_G
Cuts at: 513 1065 2067 2929 3389 3522 3873 513
Size: 552 1002 862 460 133 351 3529
Fragments arranged by size:
3529 1002 862 552 460 351 133
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6150
Fragments arranged by size:
6150 683 56
BtsI GCAGTG_nn'
Cuts at: 2566 3832 4405 5199 5219 2566
Size: 1266 573 794 20 4236
Fragments arranged by size:
4236 1266 794 573 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6889
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6854
DraI TTT'AAA
Cuts at: 4193 4193
Size: 6889
DraIII CAC_nnn'GTG
Cuts at: 2068 4163 2068
Size: 2095 4794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5896 809
Size: 507 4580 1802
Fragments arranged by size:
4580 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2311 3774
Size: 54 1425 50 275 18 259 222 1463
Cuts at: 3774 4336 5225 6854 8
Size: 562 889 1629 43
Fragments arranged by size:
1629 1463 1425 889 562 275 259 222
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4336 1537
Size: 275 2524 4090
Fragments arranged by size:
4090 2524 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2576 3389 4819 1353
Size: 1223 813 1430 3423
Fragments arranged by size:
3423 1430 1223 813
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5476 5850 5996 387
Size: 1064 138 3887 374 146 1280
Fragments arranged by size:
3887 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2631 2631
Size: 6889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2678 2893 3032 3074 3762 4092
Size: 393 408 638 215 139 42 688 330
Cuts at: 4092 4952 5549 6336 6604 1239
Size: 860 597 787 268 1524
Fragments arranged by size:
1524 860 787 688 638 597 408 393
330 268 215 139 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2048
Size: 6889
EcoO109I rG'GnC_Cy
Cuts at: 2541 2774 3397 3456 3457 3701 4002 4067 2541
Size: 233 623 59 1 244 301 65 5363
Fragments arranged by size:
5363 623 301 244 233 65 59 1
EcoRI G'AATT_C
Cuts at: 4198 4576 4198
Size: 378 6511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2980 2203
Size: 777 6112
FspI TGC'GCA
Cuts at: 5395 5395
Size: 6889
HaeII r_GCGC'y
Cuts at: 1678 4036 6036 1678
Size: 2358 2000 2531
Fragments arranged by size:
2531 2358 2000
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 3034 3066 5530
Size: 32 135 32 869 32 561 32 2464
Cuts at: 5530 5562 5604 5636 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2464 869 561 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2677 3601 4233 4479 669
Size: 988 281 739 924 632 246 3079
Fragments arranged by size:
3079 988 924 739 632 281 246
HindIII A'AGCT_T
Cuts at: 748 2180 748
Size: 1432 5457
HpaI GTT'AAC
Cuts at: 4479 4479
Size: 6889
KpnI G_GTAC'C
Cuts at: 4214 4214
Size: 6889
MfeI C'AATT_G
Cuts at: 4488 4488
Size: 6889
MluI A'CGCG_T
Cuts at: 6765 6765
Size: 6889
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6003 6187 1305
Size: 4698 184 2007
Fragments arranged by size:
4698 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2091 10
Size: 54 1425 343 259 4808
Fragments arranged by size:
4808 1425 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 3524 4847 5206 5365 518
Size: 648 1248 1110 1323 359 159 2042
Fragments arranged by size:
2042 1323 1248 1110 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2988 3611 3679 4305 4982 6130 6375 1606
Size: 1382 623 68 626 677 1148 245 2120
Fragments arranged by size:
2120 1382 1148 677 626 623 245 68
NaeI GCC'GGC
Cuts at: 3778 3778
Size: 6889
NcoI C'CATG_G
Cuts at: 513 1065 2929 513
Size: 552 1864 4473
Fragments arranged by size:
4473 1864 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6889
NgoMIV G'CCGG_C
Cuts at: 3776 3776
Size: 6889
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6889
NotI GC'GGCC_GC
Cuts at: 4336 4336
Size: 6889
NspI r_CATG'y
Cuts at: 1132 1229 3551 3763 3946 4247 5792 1132
Size: 97 2322 212 183 301 1545 2229
Fragments arranged by size:
2322 2229 1545 301 212 183 97
PasI CC'CwG_GG
Cuts at: 1348 3697 1348
Size: 2349 4540
PciI A'CATG_T
Cuts at: 3942 5788 3942
Size: 1846 5043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2068 2263 2320 2410 3311 3763 1108
Size: 902 58 195 57 90 901 452 4234
Fragments arranged by size:
4234 902 901 452 195 90 58 57
PfoI T'CCnGG_A
Cuts at: 3473 3473
Size: 6889
PmeI GTTT'AAAC
Cuts at: 4193 4193
Size: 6889
PmlI CAC'GTG
Cuts at: 3981 3981
Size: 6889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 4958 4990 6471 6503 3929
Size: 32 997 32 1481 32 4315
Fragments arranged by size:
4315 1481 997 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2541 3701 4067 2541
Size: 1160 366 5363
Fragments arranged by size:
5363 1160 366
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6889
PsiI TTA'TAA
Cuts at: 4459 4459
Size: 6889
PspOMI G'GGCC_C
Cuts at: 3456 3456
Size: 6889
PstI C_TGCA'G
Cuts at: 830 2685 2896 3684 3858 4241 830
Size: 1855 211 788 174 383 3478
Fragments arranged by size:
3478 1855 788 383 211 174
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6889
PvuII CAG'CTG
Cuts at: 2988 3611 3679 2988
Size: 623 68 6198
Fragments arranged by size:
6198 623 68
SacI G_AGCT'C
Cuts at: 2633 2633
Size: 6889
SalI G'TCGA_C
Cuts at: 1936 4231 1936
Size: 2295 4594
SanDI GG'GwC_CC
Cuts at: 3701 3701
Size: 6889
SbfI CC_TGCA'GG
Cuts at: 4241 4241
Size: 6889
ScaI AGT'ACT
Cuts at: 5137 6849 5137
Size: 1712 5177
SexAI A'CCwGG_T
Cuts at: 2256 2256
Size: 6889
SfcI C'TryA_G
Cuts at: 826 1045 2681 2892 2915 3103 3680 3854 4237
Size: 219 1636 211 23 188 577 174 383
Cuts at: 4237 5372 6053 6244 826
Size: 1135 681 191 1471
Fragments arranged by size:
1636 1471 1135 681 577 383 219 211
191 188 174 23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6889
SmaI CCC'GGG
Cuts at: 1872 4216 1872
Size: 2344 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3734 4024 4993 5894 6156 820
Size: 197 926 1791 290 969 901 262 1553
Fragments arranged by size:
1791 1553 969 926 901 290 262 197
SnaBI TAC'GTA
Cuts at: 493 4680 493
Size: 4187 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6889
SphI G_CATG'C
Cuts at: 1132 4247 1132
Size: 3115 3774
SspI AAT'ATT
Cuts at: 5 52 4813 5
Size: 47 4761 2081
Fragments arranged by size:
4761 2081 47
StuI AGG'CCT
Cuts at: 1517 4050 1517
Size: 2533 4356
StyI C'CwwG_G
Cuts at: 513 1065 2929 3253 3540 3728 3985 4328 513
Size: 552 1864 324 287 188 257 343 3074
Fragments arranged by size:
3074 1864 552 343 324 287 257 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2828 3645 4027 4123 4916 4933 5086
Size: 1255 1248 817 382 96 793 17 153
Cuts at: 5086 5271 5716 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1248 817 793 445 382 185
153 96 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5135 6847 96
Size: 274 80 33 51 1443 3158 1712 138
Fragments arranged by size:
3158 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3050 3082 3286 3318 4121 4153 6849 6881 3050
Size: 32 204 32 803 32 2696 32 3058
Fragments arranged by size:
3058 2696 803 204 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3841 1316
Size: 2525 4364
XbaI T'CTAG_A
Cuts at: 4225 4225
Size: 6889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2483 3438 3805 2483
Size: 955 367 5567
Fragments arranged by size:
5567 955 367
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6889
XmaI C'CCGG_G
Cuts at: 1870 4214 1870
Size: 2344 4545
XmnI GAAnn'nnTTC
Cuts at: 3944 5018 3944
Size: 1074 5815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4697 276
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SacII SapI SfiI SfoI
SrfI SwaI