Restriction map for
pFN21AE2067
(Circular) (Six-base) MAPSORT of: pFN21AE2067.seq Check: 7469 from: 1 to: 6883
pFN21AE2067 6883 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2694 2855 2937 3790 3805 2694
Size: 161 82 853 15 5772
Fragments arranged by size:
5772 853 161 82 15
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4693 278
Size: 53 83 186 936 3157 2468
Fragments arranged by size:
3157 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 3599 4226 1937
Size: 487 1175 627 4594
Fragments arranged by size:
4594 1175 627 487
Acc65I G'GTAC_C
Cuts at: 4204 4204
Size: 6883
AclI AA'CG_TT
Cuts at: 1563 5010 5383 1563
Size: 3447 373 3063
Fragments arranged by size:
3447 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3658 4946 6330 6598 1632
Size: 2026 1288 1384 268 1917
Fragments arranged by size:
2026 1917 1384 1288 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6686
AflIII A'CryG_T
Cuts at: 1203 3147 5782 6759 1203
Size: 1944 2635 977 1327
Fragments arranged by size:
2635 1944 1327 977
AgeI A'CCGG_T
Cuts at: 5714 5714
Size: 6883
AhdI GACnn_n'nnGTC
Cuts at: 5612 5612
Size: 6883
AleI CACnn'nnGTG
Cuts at: 1166 3189 1166
Size: 2023 4860
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3443 1184
Size: 2259 4624
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2231 2263 2231
Size: 32 6851
AlwNI CAG_nnn'CTG
Cuts at: 2604 3562 4626 6198 2604
Size: 958 1064 1572 3289
Fragments arranged by size:
3289 1572 1064 958
ApaI G_GGCC'C
Cuts at: 2263 2455 2263
Size: 192 6691
ApaLI G'TGCA_C
Cuts at: 4940 6096 4940
Size: 1156 5727
ApoI r'AATT_y
Cuts at: 1493 4127 4192 4418 4570 6679 1493
Size: 2634 65 226 152 2109 1697
Fragments arranged by size:
2634 2109 1697 226 152 65
AseI AT'TA_AT
Cuts at: 160 1032 5437 160
Size: 872 4405 1606
Fragments arranged by size:
4405 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6883
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2090 4208 1870
Size: 73 81 66 2118 4545
Fragments arranged by size:
4545 2118 81 73 66
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2939 2972 2939
Size: 33 6850
BamHI G'GATC_C
Cuts at: 4213 4580 4213
Size: 367 6516
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3242 3250 3661 4204 5659
Size: 325 217 627 1455 8 411 543 1455
Cuts at: 5659 618
Size: 1842
Fragments arranged by size:
1842 1455 1455 627 543 411 325 217
8
BanII G_rGCy'C
Cuts at: 1391 2263 2455 3035 3818 3845 3921 4094 4202
Size: 872 192 580 783 27 76 173 108
Cuts at: 4202 1391
Size: 4072
Fragments arranged by size:
4072 872 783 580 192 173 108 76
27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2299 3299 3893 928
Size: 971 400 1000 594 3918
Fragments arranged by size:
3918 1000 971 594 400
BbvCI CC'TCA_GC
Cuts at: 3989 3989
Size: 6883
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2694 2728 3059 3093 5074 5108 1903
Size: 34 757 34 331 34 1981 34 3678
Fragments arranged by size:
3678 1981 757 331 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3628 4775 5985 3628
Size: 1147 1210 4526
Fragments arranged by size:
4526 1210 1147
BclI T'GATC_A
Cuts at: 1548 2511 2559 1548
Size: 963 48 5872
Fragments arranged by size:
5872 963 48
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5494 136
Size: 107 122 71 631 605 3822 1525
Fragments arranged by size:
3822 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2919 6878 2919
Size: 3959 2924
BlpI GC'TnA_GC
Cuts at: 4300 4300
Size: 6883
Bme1580I G_kGCm'C
Cuts at: 1790 2263 2455 3247 3255 3666 4944 6100 1790
Size: 473 192 792 8 411 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 792 473 411 192 8
BmgBI CAC'GTC
Cuts at: 1318 2668 1318
Size: 1350 5533
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2606 4614 5572
Size: 416 53 83 399 24 1189 2008 958
Cuts at: 5572 442
Size: 1753
Fragments arranged by size:
2008 1753 1189 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6883
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2050 3049 3793 2050
Size: 999 744 5140
Fragments arranged by size:
5140 999 744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3020 3512 3789 5543 1239
Size: 1781 492 277 1754 2579
Fragments arranged by size:
2579 1781 1754 492 277
Bpu10I CC'TnA_GC
Cuts at: 3989 4001 3989
Size: 12 6871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5008 5873 6171 5008
Size: 865 298 5720
Fragments arranged by size:
5720 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5546 882
Size: 617 4047 2219
Fragments arranged by size:
4047 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3150 3387 4674 493
Size: 711 1946 237 1287 2702
Fragments arranged by size:
2702 1946 1287 711 237
BsaBI GATnn'nnATC
Cuts at: 3437 6877 3437
Size: 3440 3443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2342 4690 5072 5707
Size: 53 83 186 936 809 2348 382 635
Cuts at: 5707 275
Size: 1451
Fragments arranged by size:
2348 1451 936 809 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5316 5714 5988 6135 6546 5316
Size: 398 274 147 411 5653
Fragments arranged by size:
5653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 2571 2601 3080
Size: 30 165 30 828 30 310 30 479
Cuts at: 3080 3110 1178
Size: 30 4951
Fragments arranged by size:
4951 828 479 310 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2213 2236 2918 3248 3370 3668 3716 3977
Size: 1027 23 682 330 122 298 48 261
Cuts at: 3977 3980 1186
Size: 3 4089
Fragments arranged by size:
4089 1027 682 330 298 261 122 48
23 3
BseYI C'CCAG_C
Cuts at: 2448 2454 3082 3555 3817 3930 6086 6582 2448
Size: 6 628 473 262 113 2156 496 2749
Fragments arranged by size:
2749 2156 628 496 473 262 113 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2621 2672 3059 3777 3830 1163
Size: 573 885 51 387 718 53 4216
Fragments arranged by size:
4216 885 718 573 387 53 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2815 4333 5094 6122 1540
Size: 275 186 814 1518 761 1028 2301
Fragments arranged by size:
2301 1518 1028 814 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3056 4944 5029 6100 3056
Size: 1888 85 1071 3839
Fragments arranged by size:
3839 1888 1071 85
BsmI GAATG_Cn'
Cuts at: 4394 4487 4394
Size: 93 6790
BsmBI CGTCTCn'nnnn_
Cuts at: 2009 3621 3650 2009
Size: 1612 29 5242
Fragments arranged by size:
5242 1612 29
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2263 2455 3035 3056 3247 3255 3666
Size: 399 473 192 580 21 191 8 411
Cuts at: 3666 3818 3845 3921 4094 4202 4944 5029 6100
Size: 152 27 76 173 108 742 85 1071
Cuts at: 6100 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 580 473 411 399 192
191 173 152 108 85 76 27 21
8
BspEI T'CCGG_A
Cuts at: 6546 6546
Size: 6883
BspHI T'CATG_A
Cuts at: 3108 4772 6502 3108
Size: 1664 1730 3489
Fragments arranged by size:
3489 1730 1664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2694 2832 2855 2937 3333 3790 3805
Size: 1289 561 138 23 82 396 457 15
Cuts at: 3805 4238 844
Size: 433 3489
Fragments arranged by size:
3489 1289 561 457 433 396 138 82
23 15
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 4770 1394
Size: 429 572 2375 3507
Fragments arranged by size:
3507 2375 572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3573 5378 5552 65
Size: 1183 2325 1805 174 1396
Fragments arranged by size:
2325 1805 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2695 3015 5527 5714 1700
Size: 110 885 320 2512 187 2869
Fragments arranged by size:
2869 2512 885 320 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6883
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6883
BssSI C'ACGA_G
Cuts at: 4943 5955 4943
Size: 1012 5871
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2604 3791 794
Size: 1810 1187 3886
Fragments arranged by size:
3886 1810 1187
BstBI TT'CG_AA
Cuts at: 5687 5687
Size: 6883
BstEII G'GTnAC_C
Cuts at: 1184 4063 1184
Size: 2879 4004
BstYI r'GATC_y
Cuts at: 1972 2853 2919 4213 4580 4965 4982 6434 6520
Size: 881 66 1294 367 385 17 1452 86
Cuts at: 6520 6532 6878 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1294 881 385 367 346 86
66 17 12
Bsu36I CC'TnA_GG
Cuts at: 2520 2520
Size: 6883
BtgI C'CryG_G
Cuts at: 513 1065 2872 3210 513
Size: 552 1807 338 4186
Fragments arranged by size:
4186 1807 552 338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6144
Fragments arranged by size:
6144 683 56
BtsI GCAGTG_nn'
Cuts at: 2425 2542 3047 3427 4399 5193 5213 2425
Size: 117 505 380 972 794 20 4095
Fragments arranged by size:
4095 972 794 505 380 117 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6883
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6848
DraI TTT'AAA
Cuts at: 4187 4187
Size: 6883
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2471 5890 809
Size: 507 1155 3419 1802
Fragments arranged by size:
3419 1802 1155 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2286 2382 2643
Size: 54 1425 50 275 18 456 96 261
Cuts at: 2643 2697 2715 3339 4330 5219 6848 8
Size: 54 18 624 991 889 1629 43
Fragments arranged by size:
1629 1425 991 889 624 456 275 261
96 54 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4330 1537
Size: 275 2518 4090
Fragments arranged by size:
4090 2518 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2007 3068 3578 3762 4813 1353
Size: 654 1061 510 184 1051 3423
Fragments arranged by size:
3423 1061 1051 654 510 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2135 5470 5844 5990 387
Size: 1064 138 546 3335 374 146 1280
Fragments arranged by size:
3335 1280 1064 546 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3020 3512 3658 3789 4946 5543 6330
Size: 393 1388 492 146 131 1157 597 787
Cuts at: 6330 6598 1239
Size: 268 1524
Fragments arranged by size:
1524 1388 1157 787 597 492 393 268
146 131
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 2191 2216 2561 3002 2129
Size: 62 25 345 441 6010
Fragments arranged by size:
6010 441 345 62 25
EcoO109I rG'GnC_Cy
Cuts at: 2260 2728 3103 3545 2260
Size: 468 375 442 5598
Fragments arranged by size:
5598 468 442 375
EcoRI G'AATT_C
Cuts at: 4192 4570 4192
Size: 378 6505
FspI TGC'GCA
Cuts at: 5389 5389
Size: 6883
HaeII r_GCGC'y
Cuts at: 1678 6030 1678
Size: 4352 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 3521 3553 5524
Size: 32 135 32 488 32 1429 32 1971
Cuts at: 5524 5556 5598 5630 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1971 1429 488 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3952 4227 4473 669
Size: 988 281 2014 275 246 3079
Fragments arranged by size:
3079 2014 988 281 275 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6883
HpaI GTT'AAC
Cuts at: 4473 4473
Size: 6883
KpnI G_GTAC'C
Cuts at: 4208 4208
Size: 6883
MfeI C'AATT_G
Cuts at: 4482 4482
Size: 6883
MluI A'CGCG_T
Cuts at: 6759 6759
Size: 6883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 3678 5997 6181 1305
Size: 969 1404 2319 184 2007
Fragments arranged by size:
2319 2007 1404 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2288 2384 10
Size: 54 1425 343 456 96 4509
Fragments arranged by size:
4509 1425 456 343 96 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3113 3189 3389 4841 5200 5359 518
Size: 648 1947 76 200 1452 359 159 2042
Fragments arranged by size:
2042 1947 1452 648 359 200 159 76
MspA1I CmG'CkG
Cuts at: 1606 2366 2458 2604 2708 3300 3542 3613 4071
Size: 760 92 146 104 592 242 71 458
Cuts at: 4071 4299 4976 6124 6369 1606
Size: 228 677 1148 245 2120
Fragments arranged by size:
2120 1148 760 677 592 458 245 242
228 146 104 92 71
NcoI C'CATG_G
Cuts at: 513 1065 2872 513
Size: 552 1807 4524
Fragments arranged by size:
4524 1807 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6883
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6883
NotI GC'GGCC_GC
Cuts at: 4330 4330
Size: 6883
NspI r_CATG'y
Cuts at: 1132 1229 2101 2943 3272 3430 4241 5786 1132
Size: 97 872 842 329 158 811 1545 2229
Fragments arranged by size:
2229 1545 872 842 811 329 158 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6883
PciI A'CATG_T
Cuts at: 5782 5782
Size: 6883
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2256 2724 2878 3028 3562 3697 1108
Size: 1148 468 154 150 534 135 4294
Fragments arranged by size:
4294 1148 534 468 154 150 135
PfoI T'CCnGG_A
Cuts at: 2217 2433 2997 2217
Size: 216 564 6103
Fragments arranged by size:
6103 564 216
PmeI GTTT'AAAC
Cuts at: 4187 4187
Size: 6883
PmlI CAC'GTG
Cuts at: 3387 3387
Size: 6883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2231 2263 4952 4984 6465 6497 2231
Size: 32 2689 32 1481 32 2617
Fragments arranged by size:
2689 2617 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6883
PsiI TTA'TAA
Cuts at: 4453 4453
Size: 6883
PspOMI G'GGCC_C
Cuts at: 2259 2451 2259
Size: 192 6691
PstI C_TGCA'G
Cuts at: 830 2181 2829 2934 4235 830
Size: 1351 648 105 1301 3478
Fragments arranged by size:
3478 1351 1301 648 105
PvuI CG_AT'CG
Cuts at: 2001 2815 2001
Size: 814 6069
PvuII CAG'CTG
Cuts at: 2366 2458 2604 3613 2366
Size: 92 146 1009 5636
Fragments arranged by size:
5636 1009 146 92
SalI G'TCGA_C
Cuts at: 1936 4225 1936
Size: 2289 4594
SapI GCTCTTCn'nnn_
Cuts at: 3578 3762 3578
Size: 184 6699
SbfI CC_TGCA'GG
Cuts at: 2934 4235 2934
Size: 1301 5582
ScaI AGT'ACT
Cuts at: 3489 5131 6843 3489
Size: 1642 1712 3529
Fragments arranged by size:
3529 1712 1642
SfcI C'TryA_G
Cuts at: 826 1045 2177 2825 2930 3133 3600 4231 5366
Size: 219 1132 648 105 203 467 631 1135
Cuts at: 5366 6047 6238 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1132 681 648 631 467 219
203 191 105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6883
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6883
SmaI CCC'GGG
Cuts at: 1872 4210 1872
Size: 2338 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4987 5888 6150 820
Size: 197 926 3044 901 262 1553
Fragments arranged by size:
3044 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4674 493
Size: 4181 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6883
SphI G_CATG'C
Cuts at: 1132 3272 3430 4241 1132
Size: 2140 158 811 3774
Fragments arranged by size:
3774 2140 811 158
SspI AAT'ATT
Cuts at: 5 52 4807 5
Size: 47 4755 2081
Fragments arranged by size:
4755 2081 47
StuI AGG'CCT
Cuts at: 1517 2211 3999 1517
Size: 694 1788 4401
Fragments arranged by size:
4401 1788 694
StyI C'CwwG_G
Cuts at: 513 1065 2836 2872 3621 4322 513
Size: 552 1771 36 749 701 3074
Fragments arranged by size:
3074 1771 749 701 552 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2879 4051 4910 4927 5080 5265 5710
Size: 1255 1299 1172 859 17 153 185 445
Cuts at: 5710 325
Size: 1498
Fragments arranged by size:
1498 1299 1255 1172 859 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2094 3487 3705
Size: 274 80 33 51 1443 117 1393 218
Cuts at: 3705 5129 6841 96
Size: 1424 1712 138
Fragments arranged by size:
1712 1443 1424 1393 274 218 138 117
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2571 2603 6843 6875 2571
Size: 32 4240 32 2579
Fragments arranged by size:
4240 2579 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6883
XbaI T'CTAG_A
Cuts at: 4219 4219
Size: 6883
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6883
XmaI C'CCGG_G
Cuts at: 1870 4208 1870
Size: 2338 4545
XmnI GAAnn'nnTTC
Cuts at: 5012 5012
Size: 6883
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4691 276
Size: 53 83 186 936 3157 2468
Fragments arranged by size:
3157 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BstXI BstZ17I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI