Restriction map for
pFN21AE2066
(Circular) (Six-base) MAPSORT of: pFN21AE2066.seq Check: 1399 from: 1 to: 6880
pFN21AE2066 6880 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4690 278
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4223 1937
Size: 2286 4594
Acc65I G'GTAC_C
Cuts at: 4201 4201
Size: 6880
AclI AA'CG_TT
Cuts at: 1563 5007 5380 1563
Size: 3444 373 3063
Fragments arranged by size:
3444 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2181 2721 3258 3747 4130 4943 6327 6595
Size: 549 540 537 489 383 813 1384 268
Cuts at: 6595 1632
Size: 1917
Fragments arranged by size:
1917 1384 813 549 540 537 489 383
268
AflII C'TTAA_G
Cuts at: 820 1017 2819 3425 820
Size: 197 1802 606 4275
Fragments arranged by size:
4275 1802 606 197
AflIII A'CryG_T
Cuts at: 1203 5779 6756 1203
Size: 4576 977 1327
Fragments arranged by size:
4576 1327 977
AgeI A'CCGG_T
Cuts at: 5711 5711
Size: 6880
AhdI GACnn_n'nnGTC
Cuts at: 5609 5609
Size: 6880
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6880
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6880
AlwNI CAG_nnn'CTG
Cuts at: 2329 4623 6195 2329
Size: 2294 1572 3014
Fragments arranged by size:
3014 2294 1572
ApaLI G'TGCA_C
Cuts at: 2055 4937 6093 2055
Size: 2882 1156 2842
Fragments arranged by size:
2882 2842 1156
ApoI r'AATT_y
Cuts at: 1493 2491 2499 2785 2808 3904 4099 4189 4415
Size: 998 8 286 23 1096 195 90 226
Cuts at: 4415 4567 6676 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1096 998 286 226 195 152
90 23 8
AseI AT'TA_AT
Cuts at: 160 1032 5434 160
Size: 872 4402 1606
Fragments arranged by size:
4402 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
AvaI C'yCGr_G
Cuts at: 1870 1943 4205 1870
Size: 73 2262 4545
Fragments arranged by size:
4545 2262 73
BamHI G'GATC_C
Cuts at: 2319 4210 4577 2319
Size: 1891 367 4622
Fragments arranged by size:
4622 1891 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2303 4201 5656 618
Size: 325 217 627 233 283 1898 1455 1842
Fragments arranged by size:
1898 1842 1455 627 325 283 233 217
BanII G_rGCy'C
Cuts at: 1391 3518 4153 4199 1391
Size: 2127 635 46 4072
Fragments arranged by size:
4072 2127 635 46
BbeI G_GCGC'C
Cuts at: 2024 2024
Size: 6880
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2208 2916 3065 3660 4118 928
Size: 971 309 708 149 595 458 3690
Fragments arranged by size:
3690 971 708 595 458 309 149
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5071 5105 1903
Size: 34 3134 34 3678
Fragments arranged by size:
3678 3134 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2122 4772 5982 2122
Size: 2650 1210 3020
Fragments arranged by size:
3020 2650 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6880
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5491 136
Size: 107 122 71 631 605 3819 1525
Fragments arranged by size:
3819 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2869 3421 3547 6875 2869
Size: 552 126 3328 2874
Fragments arranged by size:
3328 2874 552 126
BlpI GC'TnA_GC
Cuts at: 2565 4297 2565
Size: 1732 5148
Bme1580I G_kGCm'C
Cuts at: 1790 2059 2308 4941 6097 1790
Size: 269 249 2633 1156 2573
Fragments arranged by size:
2633 2573 1156 269 249
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6880
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2767 4611 5569
Size: 416 53 83 399 24 1350 1844 958
Cuts at: 5569 442
Size: 1753
Fragments arranged by size:
1844 1753 1350 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2049 2663 3308 3452 5540 1239
Size: 810 614 645 144 2088 2579
Fragments arranged by size:
2579 2088 810 645 614 144
Bpu10I CC'TnA_GC
Cuts at: 3849 3849
Size: 6880
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2607 2852 3623 5005 5870 6168 2607
Size: 245 771 1382 865 298 3319
Fragments arranged by size:
3319 1382 865 771 298 245
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5543 882
Size: 617 4044 2219
Fragments arranged by size:
4044 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4671 493
Size: 711 3467 2702
Fragments arranged by size:
3467 2702 711
BsaBI GATnn'nnATC
Cuts at: 6874 6874
Size: 6880
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 3745 4687 5069
Size: 53 83 186 936 488 1724 942 382
Cuts at: 5069 5704 275
Size: 635 1451
Fragments arranged by size:
1724 1451 942 936 635 488 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5313 5711 5985 6132 6543 5313
Size: 398 274 147 411 5650
Fragments arranged by size:
5650 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6655
Fragments arranged by size:
6655 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2849 3740 1186
Size: 1663 891 4326
Fragments arranged by size:
4326 1663 891
BseYI C'CCAG_C
Cuts at: 3467 6083 6579 3467
Size: 2616 496 3768
Fragments arranged by size:
3768 2616 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2355 3864 1163
Size: 573 619 1509 4179
Fragments arranged by size:
4179 1509 619 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4330 5091 6119 1540
Size: 275 186 2329 761 1028 2301
Fragments arranged by size:
2329 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2059 3518 4153 4941 5026 6097 2059
Size: 1459 635 788 85 1071 2842
Fragments arranged by size:
2842 1459 1071 788 635 85
BsmI GAATG_Cn'
Cuts at: 4391 4484 4391
Size: 93 6787
BsmBI CGTCTCn'nnnn_
Cuts at: 3752 3752
Size: 6880
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2059 2308 3518 4153 4199 4941 5026
Size: 399 269 249 1210 635 46 742 85
Cuts at: 5026 6097 1391
Size: 1071 2174
Fragments arranged by size:
2174 1210 1071 742 635 399 269 249
85 46
BspEI T'CCGG_A
Cuts at: 6543 6543
Size: 6880
BspHI T'CATG_A
Cuts at: 4084 4769 6499 4084
Size: 685 1730 4465
Fragments arranged by size:
4465 1730 685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2584 4235 844
Size: 1740 1651 3489
Fragments arranged by size:
3489 1740 1651
BsrBI CCG'CTC
Cuts at: 1394 1823 4767 1394
Size: 429 2944 3507
Fragments arranged by size:
3507 2944 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5375 5549 65
Size: 1183 4127 174 1396
Fragments arranged by size:
4127 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5524 5711 1700
Size: 110 3714 187 2869
Fragments arranged by size:
3714 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6880
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6880
BssSI C'ACGA_G
Cuts at: 4940 5952 4940
Size: 1012 5868
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2405 794
Size: 1611 5269
BstBI TT'CG_AA
Cuts at: 5684 5684
Size: 6880
BstEII G'GTnAC_C
Cuts at: 1184 2480 2596 1184
Size: 1296 116 5468
Fragments arranged by size:
5468 1296 116
BstYI r'GATC_y
Cuts at: 1972 2319 2326 2869 3421 3547 4210 4577 4962
Size: 347 7 543 552 126 663 367 385
Cuts at: 4962 4979 6431 6517 6529 6875 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 663 552 543 385 367 347
346 126 86 17 12 7
Bsu36I CC'TnA_GG
Cuts at: 2016 2016
Size: 6880
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6141
Fragments arranged by size:
6141 683 56
BtsI GCAGTG_nn'
Cuts at: 4396 5190 5210 4396
Size: 794 20 6066
Fragments arranged by size:
6066 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6880
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6845
DraI TTT'AAA
Cuts at: 2137 4184 2137
Size: 2047 4833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5887 809
Size: 507 4571 1802
Fragments arranged by size:
4571 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3459 4327 5216
Size: 54 1425 50 275 18 1629 868 889
Cuts at: 5216 6845 8
Size: 1629 43
Fragments arranged by size:
1629 1629 1425 889 868 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4327 1537
Size: 275 2515 4090
Fragments arranged by size:
4090 2515 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2610 3210 4810 1353
Size: 1257 600 1600 3423
Fragments arranged by size:
3423 1600 1257 600
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2332 5467 5841 5987 387
Size: 1064 138 743 3135 374 146 1280
Fragments arranged by size:
3135 1280 1064 743 374 146 138
EcoICRI GAG'CTC
Cuts at: 3516 4151 3516
Size: 635 6245
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2181 2663 2721 3258 3308 3452
Size: 393 417 132 482 58 537 50 144
Cuts at: 3452 3747 4130 4943 5540 6327 6595 1239
Size: 295 383 813 597 787 268 1524
Fragments arranged by size:
1524 813 787 597 537 482 417 393
383 295 268 144 132 58 50
EcoRI G'AATT_C
Cuts at: 2499 4189 4567 2499
Size: 1690 378 4812
Fragments arranged by size:
4812 1690 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 6880
FspI TGC'GCA
Cuts at: 5386 5386
Size: 6880
HaeII r_GCGC'y
Cuts at: 1678 2024 6027 1678
Size: 346 4003 2531
Fragments arranged by size:
4003 2531 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2311 2343 2672 2704 3061
Size: 32 135 32 739 32 329 32 357
Cuts at: 3061 3093 3406 3425 3438 3457 3505 3537 5521
Size: 32 313 19 13 19 48 32 1984
Cuts at: 5521 5553 5595 5627 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1984 739 357 329 313 135 48
42 32 32 32 32 32 32 32
32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2173 2920 3402 4224 4470 669
Size: 988 281 235 747 482 822 246 3079
Fragments arranged by size:
3079 988 822 747 482 281 246 235
HindIII A'AGCT_T
Cuts at: 748 2506 3008 748
Size: 1758 502 4620
Fragments arranged by size:
4620 1758 502
HpaI GTT'AAC
Cuts at: 3402 4470 3402
Size: 1068 5812
KasI G'GCGC_C
Cuts at: 2020 2020
Size: 6880
KpnI G_GTAC'C
Cuts at: 4205 4205
Size: 6880
MfeI C'AATT_G
Cuts at: 3209 4479 3209
Size: 1270 5610
MluI A'CGCG_T
Cuts at: 6756 6756
Size: 6880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2322 5994 6178 1305
Size: 1017 3672 184 2007
Fragments arranged by size:
3672 2007 1017 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5058
Fragments arranged by size:
5058 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 4838 5197 5356 518
Size: 648 1416 2256 359 159 2042
Fragments arranged by size:
2256 2042 1416 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2890 3670 3984 4296 4973 6121 6366 1606
Size: 1284 780 314 312 677 1148 245 2120
Fragments arranged by size:
2120 1284 1148 780 677 314 312 245
NarI GG'CG_CC
Cuts at: 2021 2021
Size: 6880
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6328
NdeI CA'TA_TG
Cuts at: 387 3880 387
Size: 3493 3387
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6880
NotI GC'GGCC_GC
Cuts at: 4327 4327
Size: 6880
NspI r_CATG'y
Cuts at: 1132 1229 3929 4238 5783 1132
Size: 97 2700 309 1545 2229
Fragments arranged by size:
2700 2229 1545 309 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6880
PciI A'CATG_T
Cuts at: 5779 5779
Size: 6880
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2329 1108
Size: 1221 5659
PfoI T'CCnGG_A
Cuts at: 2322 2322
Size: 6880
PmeI GTTT'AAAC
Cuts at: 4184 4184
Size: 6880
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4949 4981 6462 6494 4949
Size: 32 1481 32 5335
Fragments arranged by size:
5335 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6880
PsiI TTA'TAA
Cuts at: 4450 4450
Size: 6880
PstI C_TGCA'G
Cuts at: 830 2595 3555 4232 830
Size: 1765 960 677 3478
Fragments arranged by size:
3478 1765 960 677
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6880
PvuII CAG'CTG
Cuts at: 2890 3670 2890
Size: 780 6100
SacI G_AGCT'C
Cuts at: 3518 4153 3518
Size: 635 6245
SalI G'TCGA_C
Cuts at: 1936 4222 1936
Size: 2286 4594
SbfI CC_TGCA'GG
Cuts at: 4232 4232
Size: 6880
ScaI AGT'ACT
Cuts at: 5128 6840 5128
Size: 1712 5168
SfcI C'TryA_G
Cuts at: 826 1045 2291 2591 3551 4228 5363 6044 6235
Size: 219 1246 300 960 677 1135 681 191
Cuts at: 6235 826
Size: 1471
Fragments arranged by size:
1471 1246 1135 960 681 677 300 219
191
SfoI GGC'GCC
Cuts at: 2022 2022
Size: 6880
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6880
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6880
SmaI CCC'GGG
Cuts at: 1872 4207 1872
Size: 2335 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2622 2819 2831 3425 3638 4984
Size: 197 926 679 197 12 594 213 1346
Cuts at: 4984 5885 6147 820
Size: 901 262 1553
Fragments arranged by size:
1553 1346 926 901 679 594 262 213
197 197 12
SnaBI TAC'GTA
Cuts at: 493 4671 493
Size: 4178 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6880
SphI G_CATG'C
Cuts at: 1132 4238 1132
Size: 3106 3774
SspI AAT'ATT
Cuts at: 5 52 2842 4804 5
Size: 47 2790 1962 2081
Fragments arranged by size:
2790 2081 1962 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6880
StyI C'CwwG_G
Cuts at: 513 1065 3462 4319 513
Size: 552 2397 857 3074
Fragments arranged by size:
3074 2397 857 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 4907 4924 5077 5262 5707 325
Size: 1255 1035 2292 17 153 185 445 1498
Fragments arranged by size:
2292 1498 1255 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5126 6838 96
Size: 274 80 33 51 1443 3149 1712 138
Fragments arranged by size:
3149 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6840 6872 6840
Size: 32 6848
Tth111I GACn'n_nGTC
Cuts at: 1316 2915 3559 1316
Size: 1599 644 4637
Fragments arranged by size:
4637 1599 644
XbaI T'CTAG_A
Cuts at: 3230 3544 4216 3230
Size: 314 672 5894
Fragments arranged by size:
5894 672 314
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6880
XmaI C'CCGG_G
Cuts at: 1870 4205 1870
Size: 2335 4545
XmnI GAAnn'nnTTC
Cuts at: 2033 3009 5009 2033
Size: 976 2000 3904
Fragments arranged by size:
3904 2000 976
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4688 276
Size: 53 83 186 936 3154 2468
Fragments arranged by size:
3154 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoO109I
EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI