Restriction map for
pFN21AE2065
(Circular) (Six-base) MAPSORT of: pFN21AE2065.seq Check: 1361 from: 1 to: 6862
pFN21AE2065 6862 bp
With 182 enzymes: SgfI *
July 14, 2018 22:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2136 4672 278
Size: 53 83 186 936 600 2536 2468
Fragments arranged by size:
2536 2468 936 600 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4205 1937
Size: 2268 4594
Acc65I G'GTAC_C
Cuts at: 2228 4183 2228
Size: 1955 4907
AclI AA'CG_TT
Cuts at: 1563 4989 5362 1563
Size: 3426 373 3063
Fragments arranged by size:
3426 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2230 2339 2760 3233 3695 4925 6309
Size: 464 134 109 421 473 462 1230 1384
Cuts at: 6309 6577 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1230 473 464 462 421 268
134 109
AflII C'TTAA_G
Cuts at: 820 1017 2209 820
Size: 197 1192 5473
Fragments arranged by size:
5473 1192 197
AflIII A'CryG_T
Cuts at: 1203 5761 6738 1203
Size: 4558 977 1327
Fragments arranged by size:
4558 1327 977
AgeI A'CCGG_T
Cuts at: 5693 5693
Size: 6862
AhdI GACnn_n'nnGTC
Cuts at: 5591 5591
Size: 6862
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6862
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6862
AlwNI CAG_nnn'CTG
Cuts at: 2050 2101 2475 4605 6177 2050
Size: 51 374 2130 1572 2735
Fragments arranged by size:
2735 2130 1572 374 51
ApaLI G'TGCA_C
Cuts at: 4919 6075 4919
Size: 1156 5706
ApoI r'AATT_y
Cuts at: 1493 4171 4397 4549 6658 1493
Size: 2678 226 152 2109 1697
Fragments arranged by size:
2678 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5416 160
Size: 872 4384 1606
Fragments arranged by size:
4384 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6862
AvaI C'yCGr_G
Cuts at: 1870 1943 3503 3550 4187 1870
Size: 73 1560 47 637 4545
Fragments arranged by size:
4545 1560 637 73 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2220 2253 2220
Size: 33 6829
BamHI G'GATC_C
Cuts at: 2881 4086 4192 4559 2881
Size: 1205 106 367 5184
Fragments arranged by size:
5184 1205 367 106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2228 4183 5638 618
Size: 325 217 627 248 193 1955 1455 1842
Fragments arranged by size:
1955 1842 1455 627 325 248 217 193
BanII G_rGCy'C
Cuts at: 1391 2493 3522 4181 1391
Size: 1102 1029 659 4072
Fragments arranged by size:
4072 1102 1029 659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3221 928
Size: 971 1322 4569
Fragments arranged by size:
4569 1322 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3494 3528 5053 5087 1903
Size: 34 1557 34 1525 34 3678
Fragments arranged by size:
3678 1557 1525 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4754 5964 4754
Size: 1210 5652
BclI T'GATC_A
Cuts at: 1548 2766 1548
Size: 1218 5644
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5473 136
Size: 107 122 71 631 605 3801 1525
Fragments arranged by size:
3801 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6857 6857
Size: 6862
BlpI GC'TnA_GC
Cuts at: 3398 4279 3398
Size: 881 5981
Bme1580I G_kGCm'C
Cuts at: 1790 4923 6079 1790
Size: 3133 1156 2573
Fragments arranged by size:
3133 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3937 1318
Size: 2619 4243
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2316 3479 4593
Size: 416 53 83 399 24 899 1163 1114
Cuts at: 4593 5551 442
Size: 958 1753
Fragments arranged by size:
1753 1163 1114 958 899 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6862
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3423 4129 3423
Size: 706 6156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2540 2699 2780 3056 3812 5522 1239
Size: 1301 159 81 276 756 1710 2579
Fragments arranged by size:
2579 1710 1301 756 276 159 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4987 5852 6150 4987
Size: 865 298 5699
Fragments arranged by size:
5699 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2819 3851 5525 882
Size: 617 1320 1032 1674 2219
Fragments arranged by size:
2219 1674 1320 1032 617
BsaAI yAC'GTr
Cuts at: 493 1204 4653 493
Size: 711 3449 2702
Fragments arranged by size:
3449 2702 711
BsaBI GATnn'nnATC
Cuts at: 3231 6856 3231
Size: 3625 3237
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 2745 4669 5051
Size: 53 83 186 936 600 612 1924 382
Cuts at: 5051 5686 275
Size: 635 1451
Fragments arranged by size:
1924 1451 936 635 612 600 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3780 5295 5693 5967 6114 6525 3780
Size: 1515 398 274 147 411 4117
Fragments arranged by size:
4117 1515 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2771 2801 1178
Size: 30 165 30 1368 30 5239
Fragments arranged by size:
5239 1368 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3359 3362 3570 1186
Size: 2173 3 208 4478
Fragments arranged by size:
4478 2173 208 3
BseYI C'CCAG_C
Cuts at: 2022 2050 2468 3497 3916 6065 6561 2022
Size: 28 418 1029 419 2149 496 2323
Fragments arranged by size:
2323 2149 1029 496 419 418 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2734 2831 2976 3000 1163
Size: 573 450 548 97 145 24 5025
Fragments arranged by size:
5025 573 548 450 145 97 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4312 5073 6101 1540
Size: 275 186 2311 761 1028 2301
Fragments arranged by size:
2311 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 4923 5008 6079 2493
Size: 2430 85 1071 3276
Fragments arranged by size:
3276 2430 1071 85
BsmI GAATG_Cn'
Cuts at: 4373 4466 4373
Size: 93 6769
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2493 3522 4181 4923 5008 6079 1391
Size: 399 703 1029 659 742 85 1071 2174
Fragments arranged by size:
2174 1071 1029 742 703 659 399 85
BspEI T'CCGG_A
Cuts at: 3780 6525 3780
Size: 2745 4117
BspHI T'CATG_A
Cuts at: 4751 6481 4751
Size: 1730 5132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2998 4217 844
Size: 2154 1219 3489
Fragments arranged by size:
3489 2154 1219
BsrBI CCG'CTC
Cuts at: 1394 1823 3286 4749 1394
Size: 429 1463 1463 3507
Fragments arranged by size:
3507 1463 1463 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2733 2936 5357 5531 65
Size: 1183 1485 203 2421 174 1396
Fragments arranged by size:
2421 1485 1396 1183 203 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5506 5693 1700
Size: 110 3696 187 2869
Fragments arranged by size:
3696 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6862
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6862
BssSI C'ACGA_G
Cuts at: 4922 5934 4922
Size: 1012 5850
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6862
BstBI TT'CG_AA
Cuts at: 5666 5666
Size: 6862
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6862
BstYI r'GATC_y
Cuts at: 1972 2361 2478 2498 2881 3168 4086 4192 4559
Size: 389 117 20 383 287 918 106 367
Cuts at: 4559 4944 4961 6413 6499 6511 6857 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 918 389 385 383 367 346
287 117 106 86 20 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5371
Fragments arranged by size:
5371 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6123
Fragments arranged by size:
6123 683 56
BtsI GCAGTG_nn'
Cuts at: 3765 4378 5172 5192 3765
Size: 613 794 20 5435
Fragments arranged by size:
5435 794 613 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6862
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6827
DraI TTT'AAA
Cuts at: 4166 4166
Size: 6862
DraIII CAC_nnn'GTG
Cuts at: 2340 2340
Size: 6862
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5869 809
Size: 507 4553 1802
Fragments arranged by size:
4553 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2433 4309
Size: 54 1425 50 275 18 345 258 1876
Cuts at: 4309 5198 6827 8
Size: 889 1629 43
Fragments arranged by size:
1876 1629 1425 889 345 275 258 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4309 1537
Size: 275 2497 4090
Fragments arranged by size:
4090 2497 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2246 2270 3029 4792 1353
Size: 893 24 759 1763 3423
Fragments arranged by size:
3423 1763 893 759 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5449 5823 5969 387
Size: 1064 138 3860 374 146 1280
Fragments arranged by size:
3860 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2491 2491
Size: 6862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2230 2339 2540 2699 2760 2780
Size: 393 464 134 109 201 159 61 20
Cuts at: 2780 3056 3233 3695 3812 4925 5522 6309 6577
Size: 276 177 462 117 1113 597 787 268
Cuts at: 6577 1239
Size: 1524
Fragments arranged by size:
1524 1113 787 597 464 462 393 276
268 201 177 159 134 117 109 61
20
EcoNI CCTnn'n_nnAGG
Cuts at: 2165 3331 2165
Size: 1166 5696
EcoRI G'AATT_C
Cuts at: 4171 4549 4171
Size: 378 6484
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3724 3724
Size: 6862
FspI TGC'GCA
Cuts at: 5368 5368
Size: 6862
HaeII r_GCGC'y
Cuts at: 1678 6009 1678
Size: 4331 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2642 2674 4071 4103 5503
Size: 32 135 32 1070 32 1397 32 1400
Cuts at: 5503 5535 5577 5609 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1400 1397 1070 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2806 2890 2961 3115 4206 4452
Size: 988 281 868 84 71 154 1091 246
Cuts at: 4452 669
Size: 3079
Fragments arranged by size:
3079 1091 988 868 281 246 154 84
71
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6862
HpaI GTT'AAC
Cuts at: 3115 4452 3115
Size: 1337 5525
KpnI G_GTAC'C
Cuts at: 2232 4187 2232
Size: 1955 4907
MfeI C'AATT_G
Cuts at: 4461 4461
Size: 6862
MluI A'CGCG_T
Cuts at: 6738 6738
Size: 6862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3175 5976 6160 1305
Size: 1870 2801 184 2007
Fragments arranged by size:
2801 2007 1870 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 10
Size: 54 1425 343 345 4695
Fragments arranged by size:
4695 1425 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2393 4820 5179 5338 518
Size: 648 1227 2427 359 159 2042
Fragments arranged by size:
2427 2042 1227 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 3319 3478 4278 4955 6103 6348 1606
Size: 420 1293 159 800 677 1148 245 2120
Fragments arranged by size:
2120 1293 1148 800 677 420 245 159
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5371
Fragments arranged by size:
5371 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6862
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6862
NotI GC'GGCC_GC
Cuts at: 4309 4309
Size: 6862
NspI r_CATG'y
Cuts at: 1132 1229 4220 5765 1132
Size: 97 2991 1545 2229
Fragments arranged by size:
2991 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3390 1348
Size: 2042 4820
PciI A'CATG_T
Cuts at: 5761 5761
Size: 6862
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2475 3046 1108
Size: 1367 571 4924
Fragments arranged by size:
4924 1367 571
PfoI T'CCnGG_A
Cuts at: 2064 2265 2581 3789 2064
Size: 201 316 1208 5137
Fragments arranged by size:
5137 1208 316 201
PmeI GTTT'AAAC
Cuts at: 4166 4166
Size: 6862
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4931 4963 6444 6476 4931
Size: 32 1481 32 5317
Fragments arranged by size:
5317 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2577 1613
Size: 964 5898
PsiI TTA'TAA
Cuts at: 4432 4432
Size: 6862
PstI C_TGCA'G
Cuts at: 830 2142 3588 3772 4214 830
Size: 1312 1446 184 442 3478
Fragments arranged by size:
3478 1446 1312 442 184
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6862
PvuII CAG'CTG
Cuts at: 2026 3319 3478 2026
Size: 1293 159 5410
Fragments arranged by size:
5410 1293 159
SacI G_AGCT'C
Cuts at: 2493 2493
Size: 6862
SalI G'TCGA_C
Cuts at: 1936 4204 1936
Size: 2268 4594
SbfI CC_TGCA'GG
Cuts at: 2142 4214 2142
Size: 2072 4790
ScaI AGT'ACT
Cuts at: 5110 6822 5110
Size: 1712 5150
SexAI A'CCwGG_T
Cuts at: 2954 2954
Size: 6862
SfcI C'TryA_G
Cuts at: 826 1045 2138 3584 3768 4210 5345 6026 6217
Size: 219 1093 1446 184 442 1135 681 191
Cuts at: 6217 826
Size: 1471
Fragments arranged by size:
1471 1446 1135 1093 681 442 219 191
184
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6862
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6862
SmaI CCC'GGG
Cuts at: 1872 3552 4189 1872
Size: 1680 637 4545
Fragments arranged by size:
4545 1680 637
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2209 4966 5867 6129 820
Size: 197 926 266 2757 901 262 1553
Fragments arranged by size:
2757 1553 926 901 266 262 197
SnaBI TAC'GTA
Cuts at: 493 4653 493
Size: 4160 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6862
SphI G_CATG'C
Cuts at: 1132 4220 1132
Size: 3088 3774
SspI AAT'ATT
Cuts at: 5 52 4786 5
Size: 47 4734 2081
Fragments arranged by size:
4734 2081 47
StuI AGG'CCT
Cuts at: 1517 3901 1517
Size: 2384 4478
StyI C'CwwG_G
Cuts at: 513 1065 2004 2502 3708 4040 4301 513
Size: 552 939 498 1206 332 261 3074
Fragments arranged by size:
3074 1206 939 552 498 332 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3511 3929 4889 4906 5059 5244 5689
Size: 1255 1931 418 960 17 153 185 445
Cuts at: 5689 325
Size: 1498
Fragments arranged by size:
1931 1498 1255 960 445 418 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5108 6820 96
Size: 274 80 33 51 1443 3131 1712 138
Fragments arranged by size:
3131 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2696 2728 2771 2803 3941 3973 6822 6854 2696
Size: 32 43 32 1138 32 2849 32 2704
Fragments arranged by size:
2849 2704 1138 43 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6862
XbaI T'CTAG_A
Cuts at: 4198 4198
Size: 6862
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3934 4038 3934
Size: 104 6758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6862
XmaI C'CCGG_G
Cuts at: 1870 3550 4187 1870
Size: 1680 637 4545
Fragments arranged by size:
4545 1680 637
XmnI GAAnn'nnTTC
Cuts at: 4991 4991
Size: 6862
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2134 4670 276
Size: 53 83 186 936 600 2536 2468
Fragments arranged by size:
2536 2468 936 600 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI