Restriction map for
pFN21AE2061
(Circular) (Six-base) MAPSORT of: pFN21AE2061.seq Check: 1463 from: 1 to: 6820
pFN21AE2061 6820 bp
With 182 enzymes: SgfI *
January 30, 2017 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2287 2287
Size: 6820
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2993 3898 4630 278
Size: 53 83 186 936 1457 905 732 2468
Fragments arranged by size:
2468 1457 936 905 732 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2661 4163 1937
Size: 724 1502 4594
Fragments arranged by size:
4594 1502 724
Acc65I G'GTAC_C
Cuts at: 4141 4141
Size: 6820
AclI AA'CG_TT
Cuts at: 1563 4947 5320 1563
Size: 3384 373 3063
Fragments arranged by size:
3384 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2272 2557 2558 4883 6267 6535 1632
Size: 640 285 1 2325 1384 268 1917
Fragments arranged by size:
2325 1917 1384 640 285 268 1
AfeI AGC'GCT
Cuts at: 3126 3126
Size: 6820
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6623
AflIII A'CryG_T
Cuts at: 1203 3078 5719 6696 1203
Size: 1875 2641 977 1327
Fragments arranged by size:
2641 1875 1327 977
AgeI A'CCGG_T
Cuts at: 5651 5651
Size: 6820
AhdI GACnn_n'nnGTC
Cuts at: 3585 5549 3585
Size: 1964 4856
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6820
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3530 1184
Size: 2346 4474
AlwNI CAG_nnn'CTG
Cuts at: 3256 4000 4563 6135 3256
Size: 744 563 1572 3941
Fragments arranged by size:
3941 1572 744 563
ApaI G_GGCC'C
Cuts at: 3328 3369 3683 3745 3773 3887 3328
Size: 41 314 62 28 114 6261
Fragments arranged by size:
6261 314 114 62 41 28
ApaLI G'TGCA_C
Cuts at: 4877 6033 4877
Size: 1156 5664
ApoI r'AATT_y
Cuts at: 1493 2444 2732 4129 4355 4507 6616 1493
Size: 951 288 1397 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 951 288 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5374 160
Size: 872 4342 1606
Fragments arranged by size:
4342 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
AvaI C'yCGr_G
Cuts at: 1870 1943 2141 2207 2215 3623 3880 4145 1870
Size: 73 198 66 8 1408 257 265 4545
Fragments arranged by size:
4545 1408 265 257 198 73 66 8
BamHI G'GATC_C
Cuts at: 4150 4517 4150
Size: 367 6453
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2111 3628 4141 5596
Size: 325 217 627 232 92 1517 513 1455
Cuts at: 5596 618
Size: 1842
Fragments arranged by size:
1842 1517 1455 627 513 325 232 217
92
BanII G_rGCy'C
Cuts at: 1391 2922 3231 3299 3328 3369 3683 3745 3773
Size: 1531 309 68 29 41 314 62 28
Cuts at: 3773 3887 4139 1391
Size: 114 252 4072
Fragments arranged by size:
4072 1531 314 309 252 114 68 62
41 29 28
BbeI G_GCGC'C
Cuts at: 2023 3632 2023
Size: 1609 5211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3017 3213 928
Size: 971 1118 196 4535
Fragments arranged by size:
4535 1118 971 196
BbvCI CC'TCA_GC
Cuts at: 3541 3541
Size: 6820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3848 3882 5011 5045 1903
Size: 34 1911 34 1129 34 3678
Fragments arranged by size:
3678 1911 1129 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4712 5922 4712
Size: 1210 5610
BclI T'GATC_A
Cuts at: 1548 2746 1548
Size: 1198 5622
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2183 2192 3364
Size: 107 122 71 631 605 511 9 1172
Cuts at: 3364 3678 3740 3798 3821 5431 136
Size: 314 62 58 23 1610 1525
Fragments arranged by size:
1610 1525 1172 631 605 511 314 122
107 71 62 58 23 9
BglII A'GATC_T
Cuts at: 6815 6815
Size: 6820
BlpI GC'TnA_GC
Cuts at: 2780 3375 4237 2780
Size: 595 862 5363
Fragments arranged by size:
5363 862 595
Bme1580I G_kGCm'C
Cuts at: 1790 2116 2820 3328 3369 3683 3745 3773 3837
Size: 326 704 508 41 314 62 28 64
Cuts at: 3837 3887 4881 6037 1790
Size: 50 994 1156 2573
Fragments arranged by size:
2573 1156 994 704 508 326 314 64
62 50 41 28
BmgBI CAC'GTC
Cuts at: 1318 2944 3934 1318
Size: 1626 990 4204
Fragments arranged by size:
4204 1626 990
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2389 2631 2729
Size: 416 53 83 399 24 972 242 98
Cuts at: 2729 3852 3988 4551 5509 442
Size: 1123 136 563 958 1753
Fragments arranged by size:
1753 1123 972 958 563 416 399 242
136 98 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6820
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2182
Size: 6820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2326 3056 3618 5480 1239
Size: 1087 730 562 1862 2579
Fragments arranged by size:
2579 1862 1087 730 562
Bpu10I CC'TnA_GC
Cuts at: 3249 3541 4049 3249
Size: 292 508 6020
Fragments arranged by size:
6020 508 292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4945 5810 6108 4945
Size: 865 298 5657
Fragments arranged by size:
5657 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2538 4067 5483 882
Size: 617 1039 1529 1416 2219
Fragments arranged by size:
2219 1529 1416 1039 617
BsaAI yAC'GTr
Cuts at: 493 1204 3081 4611 493
Size: 711 1877 1530 2702
Fragments arranged by size:
2702 1877 1530 711
BsaBI GATnn'nnATC
Cuts at: 6814 6814
Size: 6820
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 2990 3629 3895
Size: 53 83 186 936 487 970 639 266
Cuts at: 3895 4627 5009 5644 275
Size: 732 382 635 1451
Fragments arranged by size:
1451 970 936 732 639 635 487 382
266 186 83 53
BsaWI w'CCGG_w
Cuts at: 5253 5651 5925 6072 6483 5253
Size: 398 274 147 411 5590
Fragments arranged by size:
5590 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6595
Fragments arranged by size:
6595 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6820
BseYI C'CCAG_C
Cuts at: 2179 3351 3426 3499 3519 3549 3666 3793 3872
Size: 1172 75 73 20 30 117 127 79
Cuts at: 3872 3923 3978 4043 6023 6519 2179
Size: 51 55 65 1980 496 2480
Fragments arranged by size:
2480 1980 1172 496 127 117 79 75
73 65 55 51 30 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6247
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2196 4270 5031 6059 1540
Size: 275 186 195 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 195 186
BsiHKAI G_wGCw'C
Cuts at: 3086 3231 4881 4966 6037 3086
Size: 145 1650 85 1071 3869
Fragments arranged by size:
3869 1650 1071 145 85
BsmI GAATG_Cn'
Cuts at: 2653 4331 4424 2653
Size: 1678 93 5049
Fragments arranged by size:
5049 1678 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3666 3715 3666
Size: 49 6771
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2116 2820 2922 3086 3231 3299 3328
Size: 399 326 704 102 164 145 68 29
Cuts at: 3328 3369 3683 3745 3773 3837 3887 4139 4881
Size: 41 314 62 28 64 50 252 742
Cuts at: 4881 4966 6037 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 704 399 326 314 252
164 145 102 85 68 64 62 50
41 29 28
BspEI T'CCGG_A
Cuts at: 6483 6483
Size: 6820
BspHI T'CATG_A
Cuts at: 4709 6439 4709
Size: 1730 5090
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2022 2085 2287 4175 844
Size: 1178 63 202 1888 3489
Fragments arranged by size:
3489 1888 1178 202 63
BsrBI CCG'CTC
Cuts at: 1394 1823 3184 4707 1394
Size: 429 1361 1523 3507
Fragments arranged by size:
3507 1523 1361 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3443 5315 5489 65
Size: 1183 2195 1872 174 1396
Fragments arranged by size:
2195 1872 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3797 5464 5651 1700
Size: 110 1987 1667 187 2869
Fragments arranged by size:
2869 1987 1667 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6820
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6820
BssSI C'ACGA_G
Cuts at: 3230 4880 5892 3230
Size: 1650 1012 4158
Fragments arranged by size:
4158 1650 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6820
BstBI TT'CG_AA
Cuts at: 5624 5624
Size: 6820
BstEII G'GTnAC_C
Cuts at: 1184 3191 1184
Size: 2007 4813
BstXI CCAn_nnnn'nTGG
Cuts at: 2477 2960 2477
Size: 483 6337
BstYI r'GATC_y
Cuts at: 1972 2406 4150 4517 4902 4919 6371 6457 6469
Size: 434 1744 367 385 17 1452 86 12
Cuts at: 6469 6815 1972
Size: 346 1977
Fragments arranged by size:
1977 1744 1452 434 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 4088 4088
Size: 6820
BtgI C'CryG_G
Cuts at: 513 1065 2265 2326 2410 2799 3301 513
Size: 552 1200 61 84 389 502 4032
Fragments arranged by size:
4032 1200 552 502 389 84 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2860 3044 487
Size: 683 56 1634 184 4263
Fragments arranged by size:
4263 1634 683 184 56
BtsI GCAGTG_nn'
Cuts at: 2151 2910 3123 4336 5130 5150 2151
Size: 759 213 1213 794 20 3821
Fragments arranged by size:
3821 1213 794 759 213 20
ClaI AT'CG_AT
Cuts at: 1554 2928 1554
Size: 1374 5446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2868 2903 529
Size: 35 2304 35 4446
Fragments arranged by size:
4446 2304 35 35
DraI TTT'AAA
Cuts at: 4124 4124
Size: 6820
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2948 2995 5827 809
Size: 507 1632 47 2832 1802
Fragments arranged by size:
2832 1802 1632 507 47
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2193 3304
Size: 54 1425 50 275 18 216 147 1111
Cuts at: 3304 3605 4267 5156 6785 8
Size: 301 662 889 1629 43
Fragments arranged by size:
1629 1425 1111 889 662 301 275 216
147 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2193 4267 1537
Size: 275 381 2074 4090
Fragments arranged by size:
4090 2074 381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4115 4750 1353
Size: 2762 635 3423
Fragments arranged by size:
3423 2762 635
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2549 3714 5407 5781 5927 387
Size: 1064 138 960 1165 1693 374 146 1280
Fragments arranged by size:
1693 1280 1165 1064 960 374 146 138
EcoICRI GAG'CTC
Cuts at: 3229 3229
Size: 6820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2326 2557 2558 3056 3618 4883
Size: 393 640 54 231 1 498 562 1265
Cuts at: 4883 5480 6267 6535 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1265 787 640 597 562 498 393
268 231 54 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2983 4052 2983
Size: 1069 5751
EcoO109I rG'GnC_Cy
Cuts at: 2163 2211 3253 3324 3325 3365 3366 3422 3437
Size: 48 1042 71 1 40 1 56 15
Cuts at: 3437 3620 3680 3741 3742 3769 3827 3884 3965
Size: 183 60 61 1 27 58 57 81
Cuts at: 3965 2163
Size: 5018
Fragments arranged by size:
5018 1042 183 81 71 61 60 58
57 56 48 40 27 15 1 1
1
EcoRI G'AATT_C
Cuts at: 2444 2732 4129 4507 2444
Size: 288 1397 378 4757
Fragments arranged by size:
4757 1397 378 288
FspI TGC'GCA
Cuts at: 3115 5326 3115
Size: 2211 4609
HaeII r_GCGC'y
Cuts at: 1678 2023 3128 3433 3457 3526 3548 3632 3673
Size: 345 1105 305 24 69 22 84 41
Cuts at: 3673 3766 5967 1678
Size: 93 2201 2531
Fragments arranged by size:
2531 2201 1105 345 305 93 84 69
41 24 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3068 3093 3100 3125 3571
Size: 32 135 32 1496 25 7 25 446
Cuts at: 3571 3603 5461 5493 5535 5567 1373
Size: 32 1858 32 42 32 2626
Fragments arranged by size:
2626 1858 1496 446 135 42 32 32
32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2644 2851 3238 4164 4410 669
Size: 988 281 706 207 387 926 246 3079
Fragments arranged by size:
3079 988 926 706 387 281 246 207
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6820
HpaI GTT'AAC
Cuts at: 2644 4410 2644
Size: 1766 5054
KasI G'GCGC_C
Cuts at: 2019 3628 2019
Size: 1609 5211
KpnI G_GTAC'C
Cuts at: 4145 4145
Size: 6820
MfeI C'AATT_G
Cuts at: 4419 4419
Size: 6820
MluI A'CGCG_T
Cuts at: 6696 6696
Size: 6820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2010 2271 4140 5934 6118 1305
Size: 705 261 1869 1794 184 2007
Fragments arranged by size:
2007 1869 1794 705 261 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2048 3306 10
Size: 54 1425 343 216 1258 3524
Fragments arranged by size:
3524 1425 1258 343 216 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2475 3161 3201 4778 5137 5296 518
Size: 648 1309 686 40 1577 359 159 2042
Fragments arranged by size:
2042 1577 1309 686 648 359 159 40
MspA1I CmG'CkG
Cuts at: 1606 2183 2279 2328 3274 3503 3876 3890 3910
Size: 577 96 49 946 229 373 14 20
Cuts at: 3910 3956 4236 4913 6061 6306 1606
Size: 46 280 677 1148 245 2120
Fragments arranged by size:
2120 1148 946 677 577 373 280 245
229 96 49 46 20 14
NaeI GCC'GGC
Cuts at: 3799 3799
Size: 6820
NarI GG'CG_CC
Cuts at: 2020 3629 2020
Size: 1609 5211
NcoI C'CATG_G
Cuts at: 513 1065 2410 3301 513
Size: 552 1345 891 4032
Fragments arranged by size:
4032 1345 891 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6820
NgoMIV G'CCGG_C
Cuts at: 3797 3797
Size: 6820
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6820
NotI GC'GGCC_GC
Cuts at: 4267 4267
Size: 6820
NspI r_CATG'y
Cuts at: 1132 1229 4178 5723 1132
Size: 97 2949 1545 2229
Fragments arranged by size:
2949 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2682 3320 3583 1348
Size: 1334 638 263 4585
Fragments arranged by size:
4585 1334 638 263
PciI A'CATG_T
Cuts at: 5719 5719
Size: 6820
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6820
PfoI T'CCnGG_A
Cuts at: 2868 2868
Size: 6820
PmeI GTTT'AAAC
Cuts at: 4124 4124
Size: 6820
PmlI CAC'GTG
Cuts at: 3081 3081
Size: 6820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4889 4921 6402 6434 4889
Size: 32 1481 32 5275
Fragments arranged by size:
5275 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2163 2211 3437 3827 3965 2163
Size: 48 1226 390 138 5018
Fragments arranged by size:
5018 1226 390 138 48
PshAI GACnn'nnGTC
Cuts at: 1613 3707 1613
Size: 2094 4726
PsiI TTA'TAA
Cuts at: 4390 4390
Size: 6820
PspOMI G'GGCC_C
Cuts at: 3324 3365 3679 3741 3769 3883 3324
Size: 41 314 62 28 114 6261
Fragments arranged by size:
6261 314 114 62 41 28
PstI C_TGCA'G
Cuts at: 830 2082 2622 3961 4172 830
Size: 1252 540 1339 211 3478
Fragments arranged by size:
3478 1339 1252 540 211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6820
PvuII CAG'CTG
Cuts at: 2279 3503 3910 2279
Size: 1224 407 5189
Fragments arranged by size:
5189 1224 407
SacI G_AGCT'C
Cuts at: 3231 3231
Size: 6820
SacII CC_GC'GG
Cuts at: 2329 2329
Size: 6820
SalI G'TCGA_C
Cuts at: 1936 4162 1936
Size: 2226 4594
SanDI GG'GwC_CC
Cuts at: 3437 3827 3437
Size: 390 6430
SbfI CC_TGCA'GG
Cuts at: 4172 4172
Size: 6820
ScaI AGT'ACT
Cuts at: 5068 6780 5068
Size: 1712 5108
SfcI C'TryA_G
Cuts at: 826 1045 2078 2618 3957 4168 5303 5984 6175
Size: 219 1033 540 1339 211 1135 681 191
Cuts at: 6175 826
Size: 1471
Fragments arranged by size:
1471 1339 1135 1033 681 540 219 211
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2192 3740 3798 2192
Size: 1548 58 5214
Fragments arranged by size:
5214 1548 58
SfoI GGC'GCC
Cuts at: 2021 3630 2021
Size: 1609 5211
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6820
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6820
SmaI CCC'GGG
Cuts at: 1872 2143 2209 3625 3882 4147 1872
Size: 271 66 1416 257 265 4545
Fragments arranged by size:
4545 1416 271 265 257 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4924 5825 6087 820
Size: 197 926 2981 901 262 1553
Fragments arranged by size:
2981 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4611 493
Size: 4118 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6820
SphI G_CATG'C
Cuts at: 1132 4178 1132
Size: 3046 3774
SrfI GCCC'GGGC
Cuts at: 3882 3882
Size: 6820
SspI AAT'ATT
Cuts at: 5 52 4744 5
Size: 47 4692 2081
Fragments arranged by size:
4692 2081 47
StuI AGG'CCT
Cuts at: 1517 2978 3489 3734 1517
Size: 1461 511 245 4603
Fragments arranged by size:
4603 1461 511 245
StyI C'CwwG_G
Cuts at: 513 1065 2410 3301 3333 3490 4259 513
Size: 552 1345 891 32 157 769 3074
Fragments arranged by size:
3074 1345 891 769 552 157 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2674 3061 3865 4847 4864 5017 5202
Size: 1255 1094 387 804 982 17 153 185
Cuts at: 5202 5647 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1094 982 804 445 387 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2786 5066 6778
Size: 274 80 33 51 1443 809 2280 1712
Cuts at: 6778 96
Size: 138
Fragments arranged by size:
2280 1712 1443 809 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2122 2154 2475 2507 6780 6812 2122
Size: 32 321 32 4273 32 2130
Fragments arranged by size:
4273 2130 321 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3313 1316
Size: 1997 4823
XbaI T'CTAG_A
Cuts at: 4156 4156
Size: 6820
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2477 3907 2350
Size: 127 1430 5263
Fragments arranged by size:
5263 1430 127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6820
XmaI C'CCGG_G
Cuts at: 1870 2141 2207 3623 3880 4145 1870
Size: 271 66 1416 257 265 4545
Fragments arranged by size:
4545 1416 271 265 257 66
XmnI GAAnn'nnTTC
Cuts at: 2505 4097 4949 2505
Size: 1592 852 4376
Fragments arranged by size:
4376 1592 852
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2991 3896 4628 276
Size: 53 83 186 936 1457 905 732 2468
Fragments arranged by size:
2468 1457 936 905 732 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FalI FseI FspAI NruI NsiI PacI PspXI
PsrI RsrII SapI SexAI SwaI