Restriction map for
pFN21AE2057
(Circular) (Six-base) MAPSORT of: pFN21AE2057.seq Check: 4614 from: 1 to: 6784
pFN21AE2057 6784 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3046 3046
Size: 6784
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3312 3413 4594 278
Size: 53 83 186 936 1776 101 1181 2468
Fragments arranged by size:
2468 1776 1181 936 186 101 83 53
AccI GT'mk_AC
Cuts at: 1937 4127 1937
Size: 2190 4594
Acc65I G'GTAC_C
Cuts at: 4105 4105
Size: 6784
AclI AA'CG_TT
Cuts at: 1563 4911 5284 1563
Size: 3348 373 3063
Fragments arranged by size:
3348 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2252 2782 3485 3716 3950 4847 6231 6499
Size: 620 530 703 231 234 897 1384 268
Cuts at: 6499 1632
Size: 1917
Fragments arranged by size:
1917 1384 897 703 620 530 268 234
231
AfeI AGC'GCT
Cuts at: 2853 2853
Size: 6784
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6587
AflIII A'CryG_T
Cuts at: 1203 5683 6660 1203
Size: 4480 977 1327
Fragments arranged by size:
4480 1327 977
AgeI A'CCGG_T
Cuts at: 2729 5615 2729
Size: 2886 3898
AhdI GACnn_n'nnGTC
Cuts at: 5513 5513
Size: 6784
AleI CACnn'nnGTG
Cuts at: 1166 3725 1166
Size: 2559 4225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6784
AlwNI CAG_nnn'CTG
Cuts at: 2568 4527 6099 2568
Size: 1959 1572 3253
Fragments arranged by size:
3253 1959 1572
ApaI G_GGCC'C
Cuts at: 3038 3957 3038
Size: 919 5865
ApaLI G'TGCA_C
Cuts at: 4841 5997 4841
Size: 1156 5628
ApoI r'AATT_y
Cuts at: 1493 2596 2989 3238 4093 4319 4471 6580 1493
Size: 1103 393 249 855 226 152 2109 1697
Fragments arranged by size:
2109 1697 1103 855 393 249 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5338 160
Size: 872 4306 1606
Fragments arranged by size:
4306 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
AvaI C'yCGr_G
Cuts at: 1870 1943 2877 3969 4109 1870
Size: 73 934 1092 140 4545
Fragments arranged by size:
4545 1092 934 140 73
AvrII C'CTAG_G
Cuts at: 3949 3949
Size: 6784
BamHI G'GATC_C
Cuts at: 4114 4481 4114
Size: 367 6417
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4004 4105 5560 618
Size: 325 217 627 2217 101 1455 1842
Fragments arranged by size:
2217 1842 1455 627 325 217 101
BanII G_rGCy'C
Cuts at: 1391 2094 3038 3447 3957 4103 1391
Size: 703 944 409 510 146 4072
Fragments arranged by size:
4072 944 703 510 409 146
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2559 3405 3548 928
Size: 971 660 846 143 4164
Fragments arranged by size:
4164 971 846 660 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4975 5009 1903
Size: 34 3038 34 3678
Fragments arranged by size:
3678 3038 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3529 4676 5886 3529
Size: 1147 1210 4427
Fragments arranged by size:
4427 1210 1147
BclI T'GATC_A
Cuts at: 1548 2130 2673 2883 1548
Size: 582 543 210 5449
Fragments arranged by size:
5449 582 543 210
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5395 136
Size: 107 122 71 631 605 3723 1525
Fragments arranged by size:
3723 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2352 3666 6779 2352
Size: 1314 3113 2357
Fragments arranged by size:
3113 2357 1314
BlpI GC'TnA_GC
Cuts at: 4201 4201
Size: 6784
Bme1580I G_kGCm'C
Cuts at: 1790 3038 3957 4845 6001 1790
Size: 1248 919 888 1156 2573
Fragments arranged by size:
2573 1248 1156 919 888
BmgBI CAC'GTC
Cuts at: 1318 3940 1318
Size: 2622 4162
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2798 4515 5473
Size: 416 53 83 399 24 1381 1717 958
Cuts at: 5473 442
Size: 1753
Fragments arranged by size:
1753 1717 1381 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 2186 2275 2290 2333 3821 5444 1239
Size: 826 121 89 15 43 1488 1623 2579
Fragments arranged by size:
2579 1623 1488 826 121 89 43 15
Bpu10I CC'TnA_GC
Cuts at: 3527 3527
Size: 6784
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4909 5774 6072 4909
Size: 865 298 5621
Fragments arranged by size:
5621 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5447 882
Size: 617 3948 2219
Fragments arranged by size:
3948 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4575 493
Size: 711 3371 2702
Fragments arranged by size:
3371 2702 711
BsaBI GATnn'nnATC
Cuts at: 3716 6778 3716
Size: 3062 3722
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3309 3410 4591 4973
Size: 53 83 186 936 1776 101 1181 382
Cuts at: 4973 5608 275
Size: 635 1451
Fragments arranged by size:
1776 1451 1181 936 635 382 186 101
83 53
BsaWI w'CCGG_w
Cuts at: 2729 3714 5217 5615 5889 6036 6447 2729
Size: 985 1503 398 274 147 411 3066
Fragments arranged by size:
3066 1503 985 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2063 2093 1178
Size: 30 165 30 660 30 5869
Fragments arranged by size:
5869 660 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2365 1186
Size: 1179 5605
BseYI C'CCAG_C
Cuts at: 2705 2823 2834 3446 5987 6483 2705
Size: 118 11 612 2541 496 3006
Fragments arranged by size:
3006 2541 612 496 118 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3164 3863 1163
Size: 573 1428 699 4084
Fragments arranged by size:
4084 1428 699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 4234 4995 6023 1540
Size: 275 186 1192 1041 761 1028 2301
Fragments arranged by size:
2301 1192 1041 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 4845 4930 6001 2094
Size: 2751 85 1071 2877
Fragments arranged by size:
2877 2751 1071 85
BsiWI C'GTAC_G
Cuts at: 3042 3672 3042
Size: 630 6154
BsmI GAATG_Cn'
Cuts at: 4295 4388 4295
Size: 93 6691
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 3038 3447 3957 4103 4845 4930
Size: 399 304 944 409 510 146 742 85
Cuts at: 4930 6001 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 944 742 510 409 399 304
146 85
BspEI T'CCGG_A
Cuts at: 3714 6447 3714
Size: 2733 4051
BspHI T'CATG_A
Cuts at: 2133 2905 4673 6403 2133
Size: 772 1768 1730 2514
Fragments arranged by size:
2514 1768 1730 772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3046 3255 4139 844
Size: 2202 209 884 3489
Fragments arranged by size:
3489 2202 884 209
BsrBI CCG'CTC
Cuts at: 1394 1823 2718 3766 4671 1394
Size: 429 895 1048 905 3507
Fragments arranged by size:
3507 1048 905 895 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2284 2689 3301 5279 5453 65
Size: 1183 1036 405 612 1978 174 1396
Fragments arranged by size:
1978 1396 1183 1036 612 405 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2483 2729 3874 5428 5615 1700
Size: 110 673 246 1145 1554 187 2869
Fragments arranged by size:
2869 1554 1145 673 246 187 110
BsrGI T'GTAC_A
Cuts at: 96 3595 96
Size: 3499 3285
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6784
BssSI C'ACGA_G
Cuts at: 3819 4844 5856 3819
Size: 1025 1012 4747
Fragments arranged by size:
4747 1025 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6784
BstBI TT'CG_AA
Cuts at: 5588 5588
Size: 6784
BstEII G'GTnAC_C
Cuts at: 1184 2444 3923 1184
Size: 1260 1479 4045
Fragments arranged by size:
4045 1479 1260
BstXI CCAn_nnnn'nTGG
Cuts at: 2751 3286 3865 2751
Size: 535 579 5670
Fragments arranged by size:
5670 579 535
BstYI r'GATC_y
Cuts at: 1972 2352 2769 3666 4114 4481 4866 4883 6335
Size: 380 417 897 448 367 385 17 1452
Cuts at: 6335 6421 6433 6779 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 897 448 417 385 380 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3639 3639
Size: 6784
BtgI C'CryG_G
Cuts at: 513 1065 2004 2949 513
Size: 552 939 945 4348
Fragments arranged by size:
4348 945 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6045
Fragments arranged by size:
6045 683 56
BtsI GCAGTG_nn'
Cuts at: 4300 5094 5114 4300
Size: 794 20 5970
Fragments arranged by size:
5970 794 20
ClaI AT'CG_AT
Cuts at: 1554 4071 1554
Size: 2517 4267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6749
DraI TTT'AAA
Cuts at: 4088 4088
Size: 6784
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5791 809
Size: 507 4475 1802
Fragments arranged by size:
4475 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2625 2653
Size: 54 1425 50 275 18 375 420 28
Cuts at: 2653 2734 2815 4231 5120 6749 8
Size: 81 81 1416 889 1629 43
Fragments arranged by size:
1629 1425 1416 889 420 375 275 81
81 54 50 43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 4231 1537
Size: 275 2419 4090
Fragments arranged by size:
4090 2419 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2457 3639 3857 4714 1353
Size: 1104 1182 218 857 3423
Fragments arranged by size:
3423 1182 1104 857 218
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2591 5371 5745 5891 387
Size: 1064 138 1002 2780 374 146 1280
Fragments arranged by size:
2780 1280 1064 1002 374 146 138
EcoICRI GAG'CTC
Cuts at: 2092 2092
Size: 6784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2186 2252 2275 2290 2333 2782
Size: 393 433 121 66 23 15 43 449
Cuts at: 2782 3485 3716 3821 3950 4847 5444 6231 6499
Size: 703 231 105 129 897 597 787 268
Cuts at: 6499 1239
Size: 1524
Fragments arranged by size:
1524 897 787 703 597 449 433 393
268 231 129 121 105 66 43 23
15
EcoNI CCTnn'n_nnAGG
Cuts at: 2966 2966
Size: 6784
EcoO109I rG'GnC_Cy
Cuts at: 2186 2740 2838 3035 3736 3953 3966 2186
Size: 554 98 197 701 217 13 5004
Fragments arranged by size:
5004 701 554 217 197 98 13
EcoRI G'AATT_C
Cuts at: 3238 4093 4471 3238
Size: 855 378 5551
Fragments arranged by size:
5551 855 378
EcoRV GAT'ATC
Cuts at: 3985 3985
Size: 6784
FspI TGC'GCA
Cuts at: 5290 5290
Size: 6784
HaeII r_GCGC'y
Cuts at: 1678 2855 5931 1678
Size: 1177 3076 2531
Fragments arranged by size:
3076 2531 1177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 5425 5457 5499
Size: 32 135 32 491 32 3330 32 42
Cuts at: 5499 5531 1373
Size: 32 2626
Fragments arranged by size:
3330 2626 491 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3502 4128 4374 669
Size: 988 281 1564 626 246 3079
Fragments arranged by size:
3079 1564 988 626 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6784
HpaI GTT'AAC
Cuts at: 4374 4374
Size: 6784
KpnI G_GTAC'C
Cuts at: 4109 4109
Size: 6784
MfeI C'AATT_G
Cuts at: 4383 4383
Size: 6784
MluI A'CGCG_T
Cuts at: 6660 6660
Size: 6784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3042 5898 6082 1305
Size: 1737 2856 184 2007
Fragments arranged by size:
2856 2007 1737 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2627 2817 10
Size: 54 1425 343 795 190 3977
Fragments arranged by size:
3977 1425 795 343 190 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 3725 4742 5101 5260 518
Size: 648 1329 1230 1017 359 159 2042
Fragments arranged by size:
2042 1329 1230 1017 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3148 3725 4200 4877 6025 6270 1606
Size: 1542 577 475 677 1148 245 2120
Fragments arranged by size:
2120 1542 1148 677 577 475 245
NaeI GCC'GGC
Cuts at: 2485 2485
Size: 6784
NcoI C'CATG_G
Cuts at: 513 1065 2004 2949 513
Size: 552 939 945 4348
Fragments arranged by size:
4348 945 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6784
NgoMIV G'CCGG_C
Cuts at: 2483 2483
Size: 6784
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6784
NotI GC'GGCC_GC
Cuts at: 4231 4231
Size: 6784
NspI r_CATG'y
Cuts at: 1132 1229 3163 3481 4142 5687 1132
Size: 97 1934 318 661 1545 2229
Fragments arranged by size:
2229 1934 1545 661 318 97
PasI CC'CwG_GG
Cuts at: 1348 2362 1348
Size: 1014 5770
PciI A'CATG_T
Cuts at: 5683 5683
Size: 6784
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6784
PfoI T'CCnGG_A
Cuts at: 3777 3995 3777
Size: 218 6566
PmeI GTTT'AAAC
Cuts at: 4088 4088
Size: 6784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4853 4885 6366 6398 4853
Size: 32 1481 32 5239
Fragments arranged by size:
5239 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2740 2838 3736 3966 2740
Size: 98 898 230 5558
Fragments arranged by size:
5558 898 230 98
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6784
PsiI TTA'TAA
Cuts at: 4354 4354
Size: 6784
PspOMI G'GGCC_C
Cuts at: 3034 3953 3034
Size: 919 5865
PstI C_TGCA'G
Cuts at: 830 4136 830
Size: 3306 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6784
PvuII CAG'CTG
Cuts at: 3148 3148
Size: 6784
SacI G_AGCT'C
Cuts at: 2094 2094
Size: 6784
SalI G'TCGA_C
Cuts at: 1936 4126 1936
Size: 2190 4594
SanDI GG'GwC_CC
Cuts at: 2740 2838 3966 2740
Size: 98 1128 5558
Fragments arranged by size:
5558 1128 98
SapI GCTCTTCn'nnn_
Cuts at: 3857 3857
Size: 6784
SbfI CC_TGCA'GG
Cuts at: 4136 4136
Size: 6784
ScaI AGT'ACT
Cuts at: 5032 6744 5032
Size: 1712 5072
SexAI A'CCwGG_T
Cuts at: 3504 3504
Size: 6784
SfcI C'TryA_G
Cuts at: 826 1045 4132 5267 5948 6139 826
Size: 219 3087 1135 681 191 1471
Fragments arranged by size:
3087 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
SgrAI Cr'CCGG_yG
Cuts at: 1700 2729 1700
Size: 1029 5755
SmaI CCC'GGG
Cuts at: 1872 2879 3971 4111 1872
Size: 1007 1092 140 4545
Fragments arranged by size:
4545 1092 1007 140
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4888 5789 6051 820
Size: 197 926 2945 901 262 1553
Fragments arranged by size:
2945 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4575 493
Size: 4082 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6784
SphI G_CATG'C
Cuts at: 1132 3481 4142 1132
Size: 2349 661 3774
Fragments arranged by size:
3774 2349 661
SspI AAT'ATT
Cuts at: 5 52 4708 5
Size: 47 4656 2081
Fragments arranged by size:
4656 2081 47
StuI AGG'CCT
Cuts at: 1517 2045 2145 1517
Size: 528 100 6156
Fragments arranged by size:
6156 528 100
StyI C'CwwG_G
Cuts at: 513 1065 2004 2146 2744 2949 3088 3279 3949
Size: 552 939 142 598 205 139 191 670
Cuts at: 3949 3957 4223 513
Size: 8 266 3074
Fragments arranged by size:
3074 939 670 598 552 266 205 191
142 139 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 2931 3106 4811 4828 4981 5166
Size: 1255 758 593 175 1705 17 153 185
Cuts at: 5166 5611 325
Size: 445 1498
Fragments arranged by size:
1705 1498 1255 758 593 445 185 175
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2937 3321 3595
Size: 274 80 33 51 1443 960 384 274
Cuts at: 3595 5030 6742 96
Size: 1435 1712 138
Fragments arranged by size:
1712 1443 1435 960 384 274 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3042 3074 6744 6776 3042
Size: 32 3670 32 3050
Fragments arranged by size:
3670 3050 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6784
XbaI T'CTAG_A
Cuts at: 4120 4120
Size: 6784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3628 3796 3628
Size: 168 6616
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6784
XmaI C'CCGG_G
Cuts at: 1870 2877 3969 4109 1870
Size: 1007 1092 140 4545
Fragments arranged by size:
4545 1092 1007 140
XmnI GAAnn'nnTTC
Cuts at: 2045 2556 4913 2045
Size: 511 2357 3916
Fragments arranged by size:
3916 2357 511
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3310 3411 4592 276
Size: 53 83 186 936 1776 101 1181 2468
Fragments arranged by size:
2468 1776 1181 936 186 101 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI BplI BsmBI
BstZ17I DraIII FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI