Restriction map for
pFN21AE2054
(Circular) (Six-base) MAPSORT of: pFN21AE2054.seq Check: 4274 from: 1 to: 6580
pFN21AE2054 6580 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4390 278
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3923 1937
Size: 1986 4594
Acc65I G'GTAC_C
Cuts at: 3901 3901
Size: 6580
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4643 6027 6295 1632
Size: 3011 1384 268 1917
Fragments arranged by size:
3011 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 5479 6456 1203
Size: 4276 977 1327
Fragments arranged by size:
4276 1327 977
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 2126 1166
Size: 960 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6580
AlwNI CAG_nnn'CTG
Cuts at: 2993 3583 4323 5895 2993
Size: 590 740 1572 3678
Fragments arranged by size:
3678 1572 740 590
ApaLI G'TGCA_C
Cuts at: 4637 5793 4637
Size: 1156 5424
ApoI r'AATT_y
Cuts at: 1493 3055 3144 3177 3435 3889 4115 4267 6376
Size: 1562 89 33 258 454 226 152 2109
Cuts at: 6376 1493
Size: 1697
Fragments arranged by size:
2109 1697 1562 454 258 226 152 89
33
AseI AT'TA_AT
Cuts at: 160 1032 5134 160
Size: 872 4102 1606
Fragments arranged by size:
4102 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 2670 3213 3905 1870
Size: 73 727 543 692 4545
Fragments arranged by size:
4545 727 692 543 73
BamHI G'GATC_C
Cuts at: 3910 4277 3910
Size: 367 6213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3127 3901 5356 618
Size: 325 217 627 1340 774 1455 1842
Fragments arranged by size:
1842 1455 1340 774 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3741 3756 3899 1391
Size: 2350 15 143 4072
Fragments arranged by size:
4072 2350 143 15
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2580 3639 928
Size: 971 681 1059 3869
Fragments arranged by size:
3869 1059 971 681
BbvCI CC'TCA_GC
Cuts at: 3351 3351
Size: 6580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4771 4805 1903
Size: 34 2834 34 3678
Fragments arranged by size:
3678 2834 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 5682 4472
Size: 1210 5370
BclI T'GATC_A
Cuts at: 1548 3325 3774 1548
Size: 1777 449 4354
Fragments arranged by size:
4354 1777 449
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5191 136
Size: 107 122 71 631 605 3519 1525
Fragments arranged by size:
3519 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2438 3795 6575 2438
Size: 1357 2780 2443
Fragments arranged by size:
2780 2443 1357
BlpI GC'TnA_GC
Cuts at: 2084 3750 3997 2084
Size: 1666 247 4667
Fragments arranged by size:
4667 1666 247
Bme1580I G_kGCm'C
Cuts at: 1790 4641 5797 1790
Size: 2851 1156 2573
Fragments arranged by size:
2851 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2239 3570 3637 1318
Size: 921 1331 67 4261
Fragments arranged by size:
4261 1331 921 67
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4311 5269 442
Size: 416 53 83 399 24 2894 958 1753
Fragments arranged by size:
2894 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2312 2415 3123 3785 5240 1239
Size: 1073 103 708 662 1455 2579
Fragments arranged by size:
2579 1455 1073 708 662 103
Bpu10I CC'TnA_GC
Cuts at: 2929 3351 2929
Size: 422 6158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2419 3806 4705 5570 5868 2419
Size: 1387 899 865 298 3131
Fragments arranged by size:
3131 1387 899 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5243 882
Size: 617 3744 2219
Fragments arranged by size:
3744 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4371 493
Size: 711 3167 2702
Fragments arranged by size:
3167 2702 711
BsaBI GATnn'nnATC
Cuts at: 6574 6574
Size: 6580
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 4387 4769 5404
Size: 53 83 186 936 636 2218 382 635
Cuts at: 5404 275
Size: 1451
Fragments arranged by size:
2218 1451 936 636 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5013 5411 5685 5832 6243 5013
Size: 398 274 147 411 5350
Fragments arranged by size:
5350 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2714 2744 1178
Size: 30 165 30 1311 30 5014
Fragments arranged by size:
5014 1311 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2523 3422 1186
Size: 1337 899 4344
Fragments arranged by size:
4344 1337 899
BseYI C'CCAG_C
Cuts at: 2041 5783 6279 2041
Size: 3742 496 2342
Fragments arranged by size:
3742 2342 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2093 1163
Size: 573 357 5650
Fragments arranged by size:
5650 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2815 4030 4791 5819 1540
Size: 275 186 814 1215 761 1028 2301
Fragments arranged by size:
2301 1215 1028 814 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2865 3756 4641 4726 5797 2865
Size: 891 885 85 1071 3648
Fragments arranged by size:
3648 1071 891 885 85
BsmI GAATG_Cn'
Cuts at: 4091 4184 4091
Size: 93 6487
BsmBI CGTCTCn'nnnn_
Cuts at: 2505 3437 2505
Size: 932 5648
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2865 3741 3756 3899 4641 4726 5797
Size: 399 1075 876 15 143 742 85 1071
Cuts at: 5797 1391
Size: 2174
Fragments arranged by size:
2174 1075 1071 876 742 399 143 85
15
BspEI T'CCGG_A
Cuts at: 6243 6243
Size: 6580
BspHI T'CATG_A
Cuts at: 2148 4469 6199 2148
Size: 2321 1730 2529
Fragments arranged by size:
2529 2321 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2115 3112 3935 844
Size: 1271 997 823 3489
Fragments arranged by size:
3489 1271 997 823
BsrBI CCG'CTC
Cuts at: 1394 1823 2719 4467 1394
Size: 429 896 1748 3507
Fragments arranged by size:
3507 1748 896 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5075 5249 65
Size: 1183 3827 174 1396
Fragments arranged by size:
3827 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5224 5411 1700
Size: 110 3414 187 2869
Fragments arranged by size:
3414 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 4640 5652 4640
Size: 1012 5568
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6580
BstBI TT'CG_AA
Cuts at: 5384 5384
Size: 6580
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6580
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2336 2239
Size: 97 6483
BstYI r'GATC_y
Cuts at: 1972 2438 3795 3910 4277 4662 4679 6131 6217
Size: 466 1357 115 367 385 17 1452 86
Cuts at: 6217 6229 6575 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1357 466 385 367 346 115
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3732 513
Size: 552 2667 3361
Fragments arranged by size:
3361 2667 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5841
Fragments arranged by size:
5841 683 56
BtsI GCAGTG_nn'
Cuts at: 3396 4096 4890 4910 3396
Size: 700 794 20 5066
Fragments arranged by size:
5066 794 700 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6580
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6545
DraI TTT'AAA
Cuts at: 2968 3884 2968
Size: 916 5664
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5587 809
Size: 507 4271 1802
Fragments arranged by size:
4271 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2271 3109 4027
Size: 54 1425 50 275 18 441 838 918
Cuts at: 4027 4916 6545 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 918 889 838 441 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 3312 3399 4510 1353
Size: 1116 843 87 1111 3423
Fragments arranged by size:
3423 1116 1111 843 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2041 2643 5167 5541 5687 387
Size: 1064 138 452 602 2524 374 146 1280
Fragments arranged by size:
2524 1280 1064 602 452 374 146 138
EcoICRI GAG'CTC
Cuts at: 3754 3754
Size: 6580
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 2415 3123 3785 4643 5240 6027
Size: 393 680 103 708 662 858 597 787
Cuts at: 6027 6295 1239
Size: 268 1524
Fragments arranged by size:
1524 858 787 708 680 662 597 393
268 103
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 3704 2225
Size: 1479 5101
EcoO109I rG'GnC_Cy
Cuts at: 2155 2872 3697 2155
Size: 717 825 5038
Fragments arranged by size:
5038 825 717
EcoRI G'AATT_C
Cuts at: 3177 3889 4267 3177
Size: 712 378 5490
Fragments arranged by size:
5490 712 378
FspI TGC'GCA
Cuts at: 5086 5086
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 2042 3245 5727 1678
Size: 364 1203 2482 2531
Fragments arranged by size:
2531 2482 1203 364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2714 2746 2903
Size: 32 135 32 593 32 517 32 157
Cuts at: 2903 2935 3146 3178 5221 5253 5295 5327 1373
Size: 32 211 32 2043 32 42 32 2626
Fragments arranged by size:
2626 2043 593 517 211 157 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3924 4170 669
Size: 988 281 1986 246 3079
Fragments arranged by size:
3079 1986 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6580
HpaI GTT'AAC
Cuts at: 4170 4170
Size: 6580
KpnI G_GTAC'C
Cuts at: 3905 3905
Size: 6580
MfeI C'AATT_G
Cuts at: 4179 4179
Size: 6580
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3189 5694 5878 1305
Size: 1884 2505 184 2007
Fragments arranged by size:
2505 2007 1884 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2273 3111 10
Size: 54 1425 343 441 838 3479
Fragments arranged by size:
3479 1425 838 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2237 4538 4897 5056 518
Size: 648 960 111 2301 359 159 2042
Fragments arranged by size:
2301 2042 960 648 359 159 111
MspA1I CmG'CkG
Cuts at: 1606 2068 3772 3996 4673 5821 6066 1606
Size: 462 1704 224 677 1148 245 2120
Fragments arranged by size:
2120 1704 1148 677 462 245 224
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6028
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 3938 5483 1132
Size: 97 2709 1545 2229
Fragments arranged by size:
2709 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3604 1348
Size: 2256 4324
PciI A'CATG_T
Cuts at: 5479 5479
Size: 6580
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3847 1108
Size: 2739 3841
PfoI T'CCnGG_A
Cuts at: 3627 3627
Size: 6580
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2872 2872
Size: 6580
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 4150 4150
Size: 6580
PstI C_TGCA'G
Cuts at: 830 3663 3705 3932 830
Size: 2833 42 227 3478
Fragments arranged by size:
3478 2833 227 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
PvuII CAG'CTG
Cuts at: 3772 3772
Size: 6580
SacI G_AGCT'C
Cuts at: 3756 3756
Size: 6580
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 4828 6540 4828
Size: 1712 4868
SfcI C'TryA_G
Cuts at: 826 1045 3659 3701 3928 5063 5744 5935 826
Size: 219 2614 42 227 1135 681 191 1471
Fragments arranged by size:
2614 1471 1135 681 227 219 191 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 2672 3215 3907 1872
Size: 800 543 692 4545
Fragments arranged by size:
4545 800 692 543
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2434 3821 4684 5585 5847 820
Size: 197 926 491 1387 863 901 262 1553
Fragments arranged by size:
1553 1387 926 901 863 491 262 197
SnaBI TAC'GTA
Cuts at: 493 4371 493
Size: 3878 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6580
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SrfI GCCC'GGGC
Cuts at: 2672 2672
Size: 6580
SspI AAT'ATT
Cuts at: 5 52 4504 5
Size: 47 4452 2081
Fragments arranged by size:
4452 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6580
StyI C'CwwG_G
Cuts at: 513 1065 2616 2976 3546 4019 513
Size: 552 1551 360 570 473 3074
Fragments arranged by size:
3074 1551 570 552 473 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2817 3493 4607 4624 4777 4962 5407
Size: 1255 1237 676 1114 17 153 185 445
Cuts at: 5407 325
Size: 1498
Fragments arranged by size:
1498 1255 1237 1114 676 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2534 4826 6538
Size: 274 80 33 51 1443 557 2292 1712
Cuts at: 6538 96
Size: 138
Fragments arranged by size:
2292 1712 1443 557 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6540 6572 6540
Size: 32 6548
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6580
XbaI T'CTAG_A
Cuts at: 3916 3916
Size: 6580
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3058 3058
Size: 6580
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6580
XmaI C'CCGG_G
Cuts at: 1870 2670 3213 3905 1870
Size: 800 543 692 4545
Fragments arranged by size:
4545 800 692 543
XmnI GAAnn'nnTTC
Cuts at: 4709 4709
Size: 6580
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4388 276
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SwaI