Restriction map for
pFN21AE2053
(Circular) (Six-base) MAPSORT of: pFN21AE2053.seq Check: 9387 from: 1 to: 6769
pFN21AE2053 6769 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4579 278
Size: 53 83 186 936 3043 2468
Fragments arranged by size:
3043 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4112 1937
Size: 2175 4594
Acc65I G'GTAC_C
Cuts at: 4090 4090
Size: 6769
AclI AA'CG_TT
Cuts at: 1563 4896 5269 1563
Size: 3333 373 3063
Fragments arranged by size:
3333 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2160 2256 4832 6216 6484 1632
Size: 528 96 2576 1384 268 1917
Fragments arranged by size:
2576 1917 1384 528 268 96
AfeI AGC'GCT
Cuts at: 3393 3393
Size: 6769
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6572
AflIII A'CryG_T
Cuts at: 1203 2995 5668 6645 1203
Size: 1792 2673 977 1327
Fragments arranged by size:
2673 1792 1327 977
AgeI A'CCGG_T
Cuts at: 5600 5600
Size: 6769
AhdI GACnn_n'nnGTC
Cuts at: 3635 5498 3635
Size: 1863 4906
AleI CACnn'nnGTG
Cuts at: 1166 3476 1166
Size: 2310 4459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6769
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2621 2653 2621
Size: 32 6737
AlwNI CAG_nnn'CTG
Cuts at: 4512 6084 4512
Size: 1572 5197
ApaI G_GGCC'C
Cuts at: 3251 3975 3251
Size: 724 6045
ApaLI G'TGCA_C
Cuts at: 4826 5982 4826
Size: 1156 5613
ApoI r'AATT_y
Cuts at: 1493 3941 4078 4304 4456 6565 1493
Size: 2448 137 226 152 2109 1697
Fragments arranged by size:
2448 2109 1697 226 152 137
AseI AT'TA_AT
Cuts at: 160 1032 3399 5323 160
Size: 872 2367 1924 1606
Fragments arranged by size:
2367 1924 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6769
AvaI C'yCGr_G
Cuts at: 1870 1943 4094 1870
Size: 73 2151 4545
Fragments arranged by size:
4545 2151 73
BamHI G'GATC_C
Cuts at: 3326 3922 4099 4466 3326
Size: 596 177 367 5629
Fragments arranged by size:
5629 596 367 177
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3659 4090 5545 618
Size: 325 217 627 1872 431 1455 1842
Fragments arranged by size:
1872 1842 1455 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 3251 3975 3996 4088 1391
Size: 1860 724 21 92 4072
Fragments arranged by size:
4072 1860 724 92 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2901 3123 3355 4040 928
Size: 971 1002 222 232 685 3657
Fragments arranged by size:
3657 1002 971 685 232 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4960 4994 1903
Size: 34 3023 34 3678
Fragments arranged by size:
3678 3023 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4661 5871 4661
Size: 1210 5559
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6769
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3657 5380 136
Size: 107 122 71 631 605 1985 1723 1525
Fragments arranged by size:
1985 1723 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6764 6764
Size: 6769
BlpI GC'TnA_GC
Cuts at: 2049 4186 2049
Size: 2137 4632
Bme1580I G_kGCm'C
Cuts at: 1790 3251 3975 4830 5986 1790
Size: 1461 724 855 1156 2573
Fragments arranged by size:
2573 1461 1156 855 724
BmgBI CAC'GTC
Cuts at: 1318 2996 1318
Size: 1678 5091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2097 3418 3611
Size: 416 53 83 399 24 680 1321 193
Cuts at: 3611 3970 4500 5458 442
Size: 359 530 958 1753
Fragments arranged by size:
1753 1321 958 680 530 416 399 359
193 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6769
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2820 2820
Size: 6769
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 3882 5429 1239
Size: 1067 1576 1547 2579
Fragments arranged by size:
2579 1576 1547 1067
Bpu10I CC'TnA_GC
Cuts at: 3519 3519
Size: 6769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2587 3734 4894 5759 6057 2587
Size: 1147 1160 865 298 3299
Fragments arranged by size:
3299 1160 1147 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5432 882
Size: 617 3933 2219
Fragments arranged by size:
3933 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4560 493
Size: 711 3356 2702
Fragments arranged by size:
3356 2702 711
BsaBI GATnn'nnATC
Cuts at: 6763 6763
Size: 6769
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4576 4958 5593 275
Size: 53 83 186 936 3043 382 635 1451
Fragments arranged by size:
3043 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2622 5202 5600 5874 6021 6432 2622
Size: 2580 398 274 147 411 2959
Fragments arranged by size:
2959 2580 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 2236 2266 2456
Size: 30 165 30 657 30 146 30 190
Cuts at: 2456 2486 2621 2651 3625 3655 1178
Size: 30 135 30 974 30 4292
Fragments arranged by size:
4292 974 657 190 165 146 135 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2275 3179 3207 3879 1186
Size: 1089 904 28 672 4076
Fragments arranged by size:
4076 1089 904 672 28
BseYI C'CCAG_C
Cuts at: 3228 3244 3711 5972 6468 3228
Size: 16 467 2261 496 3529
Fragments arranged by size:
3529 2261 496 467 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2532 2975 3500 1163
Size: 573 498 298 443 525 4432
Fragments arranged by size:
4432 573 525 498 443 298
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4219 4980 6008 1540
Size: 275 186 2218 761 1028 2301
Fragments arranged by size:
2301 2218 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3131 4830 4915 5986 3131
Size: 1699 85 1071 3914
Fragments arranged by size:
3914 1699 1071 85
BsmI GAATG_Cn'
Cuts at: 2411 3084 4280 4373 2411
Size: 673 1196 93 4807
Fragments arranged by size:
4807 1196 673 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3643 3643
Size: 6769
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3131 3251 3975 3996 4088 4830 4915
Size: 399 1341 120 724 21 92 742 85
Cuts at: 4915 5986 1391
Size: 1071 2174
Fragments arranged by size:
2174 1341 1071 742 724 399 120 92
85 21
BspEI T'CCGG_A
Cuts at: 6432 6432
Size: 6769
BspHI T'CATG_A
Cuts at: 4658 6388 4658
Size: 1730 5039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4124 844
Size: 3280 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4656 1394
Size: 429 2833 3507
Fragments arranged by size:
3507 2833 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3040 3591 5264 5438 65
Size: 1183 1792 551 1673 174 1396
Fragments arranged by size:
1792 1673 1396 1183 551 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5413 5600 1700
Size: 110 3603 187 2869
Fragments arranged by size:
3603 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3916 96
Size: 3820 2949
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6769
BssSI C'ACGA_G
Cuts at: 4829 5841 4829
Size: 1012 5757
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6769
BstBI TT'CG_AA
Cuts at: 5573 5573
Size: 6769
BstEII G'GTnAC_C
Cuts at: 1184 2650 3762 1184
Size: 1466 1112 4191
Fragments arranged by size:
4191 1466 1112
BstYI r'GATC_y
Cuts at: 1972 2070 2619 2872 3326 3922 4099 4466 4851
Size: 98 549 253 454 596 177 367 385
Cuts at: 4851 4868 6320 6406 6418 6764 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 596 549 454 385 367 346
253 177 98 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2091 2889 2091
Size: 798 5971
BtgI C'CryG_G
Cuts at: 513 1065 3377 513
Size: 552 2312 3905
Fragments arranged by size:
3905 2312 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6030
Fragments arranged by size:
6030 683 56
BtsI GCAGTG_nn'
Cuts at: 2536 2544 4285 5079 5099 2536
Size: 8 1741 794 20 4206
Fragments arranged by size:
4206 1741 794 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6769
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6734
DraI TTT'AAA
Cuts at: 4073 4073
Size: 6769
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5776 809
Size: 507 4460 1802
Fragments arranged by size:
4460 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4216 5105 6734
Size: 54 1425 50 275 18 2386 889 1629
Cuts at: 6734 8
Size: 43
Fragments arranged by size:
2386 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4216 1537
Size: 275 2404 4090
Fragments arranged by size:
4090 2404 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2289 2349 3670 3722 4699 1353
Size: 936 60 1321 52 977 3423
Fragments arranged by size:
3423 1321 977 936 60 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5356 5730 5876 387
Size: 1064 138 3767 374 146 1280
Fragments arranged by size:
3767 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 2256 2306 3882 4832 5429 6216
Size: 393 528 96 50 1576 950 597 787
Cuts at: 6216 6484 1239
Size: 268 1524
Fragments arranged by size:
1576 1524 950 787 597 528 393 268
96 50
EcoO109I rG'GnC_Cy
Cuts at: 3629 3691 3906 3971 3972 3984 3629
Size: 62 215 65 1 12 6414
Fragments arranged by size:
6414 215 65 62 12 1
EcoRI G'AATT_C
Cuts at: 4078 4456 4078
Size: 378 6391
EcoRV GAT'ATC
Cuts at: 2812 2812
Size: 6769
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 6769
FspI TGC'GCA
Cuts at: 5275 5275
Size: 6769
HaeII r_GCGC'y
Cuts at: 1678 3395 5916 1678
Size: 1717 2521 2531
Fragments arranged by size:
2531 2521 1717
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2554 2586 2950
Size: 32 135 32 488 32 462 32 364
Cuts at: 2950 2982 3409 3441 3499 3531 3621 3653 5410
Size: 32 427 32 58 32 90 32 1757
Cuts at: 5410 5442 5484 5516 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1757 488 462 427 364 135 90
58 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3068 4021 4113 4359 669
Size: 988 281 1130 953 92 246 3079
Fragments arranged by size:
3079 1130 988 953 281 246 92
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6769
HpaI GTT'AAC
Cuts at: 4359 4359
Size: 6769
KpnI G_GTAC'C
Cuts at: 4094 4094
Size: 6769
MfeI C'AATT_G
Cuts at: 2286 4368 2286
Size: 2082 4687
MluI A'CGCG_T
Cuts at: 6645 6645
Size: 6769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2607 3693 5883 6067 1305
Size: 1302 1086 2190 184 2007
Fragments arranged by size:
2190 2007 1302 1086 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4947
Fragments arranged by size:
4947 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3476 4727 5086 5245 518
Size: 648 2310 1251 359 159 2042
Fragments arranged by size:
2310 2042 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2687 2696 3953 4185 4862 6010 6255 1606
Size: 1081 9 1257 232 677 1148 245 2120
Fragments arranged by size:
2120 1257 1148 1081 677 245 232 9
NcoI C'CATG_G
Cuts at: 513 1065 3377 513
Size: 552 2312 3905
Fragments arranged by size:
3905 2312 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6769
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6769
NotI GC'GGCC_GC
Cuts at: 4216 4216
Size: 6769
NspI r_CATG'y
Cuts at: 1132 1229 3527 4127 5672 1132
Size: 97 2298 600 1545 2229
Fragments arranged by size:
2298 2229 1545 600 97
PasI CC'CwG_GG
Cuts at: 1348 2771 1348
Size: 1423 5346
PciI A'CATG_T
Cuts at: 5668 5668
Size: 6769
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2647 3278 3383 1108
Size: 1539 631 105 4494
Fragments arranged by size:
4494 1539 631 105
PfoI T'CCnGG_A
Cuts at: 3538 3538
Size: 6769
PmeI GTTT'AAAC
Cuts at: 4073 4073
Size: 6769
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2621 2653 4838 4870 6351 6383 2621
Size: 32 2185 32 1481 32 3007
Fragments arranged by size:
3007 2185 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3629 3691 3984 3629
Size: 62 293 6414
Fragments arranged by size:
6414 293 62
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6769
PsiI TTA'TAA
Cuts at: 4339 4339
Size: 6769
PspOMI G'GGCC_C
Cuts at: 3247 3971 3247
Size: 724 6045
PstI C_TGCA'G
Cuts at: 830 4121 830
Size: 3291 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6769
PvuII CAG'CTG
Cuts at: 2687 3953 2687
Size: 1266 5503
SalI G'TCGA_C
Cuts at: 1936 4111 1936
Size: 2175 4594
SanDI GG'GwC_CC
Cuts at: 3629 3629
Size: 6769
SapI GCTCTTCn'nnn_
Cuts at: 2349 2349
Size: 6769
SbfI CC_TGCA'GG
Cuts at: 4121 4121
Size: 6769
ScaI AGT'ACT
Cuts at: 3407 5017 6729 3407
Size: 1610 1712 3447
Fragments arranged by size:
3447 1712 1610
SfcI C'TryA_G
Cuts at: 826 1045 3768 4117 5252 5933 6124 826
Size: 219 2723 349 1135 681 191 1471
Fragments arranged by size:
2723 1471 1135 681 349 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6769
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6769
SmaI CCC'GGG
Cuts at: 1872 4096 1872
Size: 2224 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2602 3749 4873 5774 6036 820
Size: 197 926 659 1147 1124 901 262 1553
Fragments arranged by size:
1553 1147 1124 926 901 659 262 197
SnaBI TAC'GTA
Cuts at: 493 4560 493
Size: 4067 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6769
SphI G_CATG'C
Cuts at: 1132 4127 1132
Size: 2995 3774
SspI AAT'ATT
Cuts at: 5 52 2489 2785 4693 5
Size: 47 2437 296 1908 2081
Fragments arranged by size:
2437 2081 1908 296 47
StuI AGG'CCT
Cuts at: 1517 3830 1517
Size: 2313 4456
StyI C'CwwG_G
Cuts at: 513 1065 3377 4208 513
Size: 552 2312 831 3074
Fragments arranged by size:
3074 2312 831 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2183 3220 3400 4796 4813 4966 5151
Size: 1255 603 1037 180 1396 17 153 185
Cuts at: 5151 5596 325
Size: 445 1498
Fragments arranged by size:
1498 1396 1255 1037 603 445 185 180
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3405 3916 5015
Size: 274 80 33 51 1443 1428 511 1099
Cuts at: 5015 6727 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1428 1099 511 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2236 2268 3625 3657 6729 6761 2236
Size: 32 1357 32 3072 32 2244
Fragments arranged by size:
3072 2244 1357 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6769
XbaI T'CTAG_A
Cuts at: 4105 4105
Size: 6769
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6769
XmaI C'CCGG_G
Cuts at: 1870 4094 1870
Size: 2224 4545
XmnI GAAnn'nnTTC
Cuts at: 2636 4898 2636
Size: 2262 4507
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4577 276
Size: 53 83 186 936 3043 2468
Fragments arranged by size:
3043 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI
SwaI XcmI