Restriction map for
pFN21AE2051
(Circular) (Six-base) MAPSORT of: pFN21AE2051.seq Check: 2789 from: 1 to: 6556
pFN21AE2051 6556 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2121 2121
Size: 6556
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3474 3543 3899 1937
Size: 1537 69 356 4594
Fragments arranged by size:
4594 1537 356 69
Acc65I G'GTAC_C
Cuts at: 2247 3877 2247
Size: 1630 4926
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2288 4619 6003 6271 1632
Size: 641 15 2331 1384 268 1917
Fragments arranged by size:
2331 1917 1384 641 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6359
AflIII A'CryG_T
Cuts at: 1203 2592 3202 3546 5455 6432 1203
Size: 1389 610 344 1909 977 1327
Fragments arranged by size:
1909 1389 1327 977 610 344
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2774 1184
Size: 1590 4966
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 6524
AlwNI CAG_nnn'CTG
Cuts at: 2716 2920 4299 5871 2716
Size: 204 1379 1572 3401
Fragments arranged by size:
3401 1572 1379 204
ApaLI G'TGCA_C
Cuts at: 3173 4613 5769 3173
Size: 1440 1156 3960
Fragments arranged by size:
3960 1440 1156
ApoI r'AATT_y
Cuts at: 1493 2376 3865 4091 4243 6352 1493
Size: 883 1489 226 152 2109 1697
Fragments arranged by size:
2109 1697 1489 883 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 2364 2762 2998 3359 3375 3758 3881
Size: 73 421 398 236 361 16 383 123
Cuts at: 3881 1870
Size: 4545
Fragments arranged by size:
4545 421 398 383 361 236 123 73
16
BamHI G'GATC_C
Cuts at: 3886 4253 3886
Size: 367 6189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2247 3877 5332 618
Size: 325 217 627 460 1630 1455 1842
Fragments arranged by size:
1842 1630 1455 627 460 325 217
BanII G_rGCy'C
Cuts at: 1391 2090 3432 3875 1391
Size: 699 1342 443 4072
Fragments arranged by size:
4072 1342 699 443
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2276 2595 2697 3402 928
Size: 971 377 319 102 705 4082
Fragments arranged by size:
4082 971 705 377 319 102
BbvCI CC'TCA_GC
Cuts at: 2075 2075
Size: 6556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3739 3773 4747 4781 1903
Size: 34 50 34 1718 34 974 34 3678
Fragments arranged by size:
3678 1718 974 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2085 3371 5167
Size: 107 122 71 631 605 413 1286 1796
Cuts at: 5167 136
Size: 1525
Fragments arranged by size:
1796 1525 1286 631 605 413 122 107
71
BglII A'GATC_T
Cuts at: 6551 6551
Size: 6556
BlpI GC'TnA_GC
Cuts at: 2816 3783 3973 2816
Size: 967 190 5399
Fragments arranged by size:
5399 967 190
Bme1580I G_kGCm'C
Cuts at: 1790 3177 3303 4617 5773 1790
Size: 1387 126 1314 1156 2573
Fragments arranged by size:
2573 1387 1314 1156 126
BmgBI CAC'GTC
Cuts at: 1318 2170 3088 1318
Size: 852 918 4786
Fragments arranged by size:
4786 918 852
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2198 4287 5245
Size: 416 53 83 399 24 781 2089 958
Cuts at: 5245 442
Size: 1753
Fragments arranged by size:
2089 1753 958 781 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2825 2897 5216 1239
Size: 1586 72 2319 2579
Fragments arranged by size:
2579 2319 1586 72
Bpu10I CC'TnA_GC
Cuts at: 2075 2075
Size: 6556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2372 2485 4681 5546 5844 2372
Size: 113 2196 865 298 3084
Fragments arranged by size:
3084 2196 865 298 113
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5219 882
Size: 617 3720 2219
Fragments arranged by size:
3720 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3205 3304 3547 4347 493
Size: 711 2001 99 243 800 2702
Fragments arranged by size:
2702 2001 800 711 243 99
BsaBI GATnn'nnATC
Cuts at: 6550 6550
Size: 6556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2273 4363 4745 5380
Size: 53 83 186 936 740 2090 382 635
Cuts at: 5380 275
Size: 1451
Fragments arranged by size:
2090 1451 936 740 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3831 4989 5387 5661 5808 6219 3831
Size: 1158 398 274 147 411 4168
Fragments arranged by size:
4168 1158 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2555 2585 3596 3626 1178
Size: 30 165 30 1152 30 1011 30 4108
Fragments arranged by size:
4108 1152 1011 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3521 1186
Size: 2335 4221
BseYI C'CCAG_C
Cuts at: 3651 5759 6255 3651
Size: 2108 496 3952
Fragments arranged by size:
3952 2108 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2734 2818 2890 3326 3335 1163
Size: 573 998 84 72 436 9 4384
Fragments arranged by size:
4384 998 573 436 84 72 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3465 4006 4767 5795 1540
Size: 275 186 1464 541 761 1028 2301
Fragments arranged by size:
2301 1464 1028 761 541 275 186
BsiHKAI G_wGCw'C
Cuts at: 3177 4617 4702 5773 3177
Size: 1440 85 1071 3960
Fragments arranged by size:
3960 1440 1071 85
BsmI GAATG_Cn'
Cuts at: 4067 4160 4067
Size: 93 6463
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 3177 3303 3432 3875 4617 4702
Size: 399 300 1087 126 129 443 742 85
Cuts at: 4702 5773 1391
Size: 1071 2174
Fragments arranged by size:
2174 1087 1071 742 443 399 300 129
126 85
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 2673 4445 6175 2673
Size: 1772 1730 3054
Fragments arranged by size:
3054 1772 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 3911 844
Size: 1277 1790 3489
Fragments arranged by size:
3489 1790 1277
BsrBI CCG'CTC
Cuts at: 1394 1823 2668 2809 2927 4443 1394
Size: 429 845 141 118 1516 3507
Fragments arranged by size:
3507 1516 845 429 141 118
BsrDI GCAATG_nn'
Cuts at: 65 1248 2973 3144 3156 3216 5051 5225 65
Size: 1183 1725 171 12 60 1835 174 1396
Fragments arranged by size:
1835 1725 1396 1183 174 171 60 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 2318 5200 5387 1700
Size: 110 241 267 2882 187 2869
Fragments arranged by size:
2882 2869 267 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6556
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 2081 4616 5628 2081
Size: 2535 1012 3009
Fragments arranged by size:
3009 2535 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3313 794
Size: 2519 4037
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 2020 2776 1184
Size: 836 756 4964
Fragments arranged by size:
4964 836 756
BstXI CCAn_nnnn'nTGG
Cuts at: 2560 2788 2560
Size: 228 6328
BstYI r'GATC_y
Cuts at: 1972 2196 3886 4253 4638 4655 6107 6193 6205
Size: 224 1690 367 385 17 1452 86 12
Cuts at: 6205 6551 1972
Size: 346 1977
Fragments arranged by size:
1977 1690 1452 385 367 346 224 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2339 3574 2339
Size: 1235 5321
BtgI C'CryG_G
Cuts at: 513 1065 2025 2278 2781 3627 3660 3735 513
Size: 552 960 253 503 846 33 75 3334
Fragments arranged by size:
3334 960 846 552 503 253 75 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2337 2573 487
Size: 683 56 1111 236 4470
Fragments arranged by size:
4470 1111 683 236 56
BtsI GCAGTG_nn'
Cuts at: 4072 4866 4886 4072
Size: 794 20 5742
Fragments arranged by size:
5742 794 20
ClaI AT'CG_AT
Cuts at: 1554 2434 2472 2883 1554
Size: 880 38 411 5227
Fragments arranged by size:
5227 880 411 38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6521
DraI TTT'AAA
Cuts at: 3860 3860
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3086 5563 809
Size: 507 1770 2477 1802
Fragments arranged by size:
2477 1802 1770 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2922 2955
Size: 54 1425 50 275 18 198 894 33
Cuts at: 2955 3054 3462 3673 3849 4003 4892 6521 8
Size: 99 408 211 176 154 889 1629 43
Fragments arranged by size:
1629 1425 894 889 408 275 211 198
176 154 99 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3462 4003 1537
Size: 275 1650 541 4090
Fragments arranged by size:
4090 1650 541 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3696 4486 1353
Size: 2343 790 3423
Fragments arranged by size:
3423 2343 790
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3637 5143 5517 5663 387
Size: 1064 138 2048 1506 374 146 1280
Fragments arranged by size:
2048 1506 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2288 2825 2897 4619 5216 6003
Size: 393 641 15 537 72 1722 597 787
Cuts at: 6003 6271 1239
Size: 268 1524
Fragments arranged by size:
1722 1524 787 641 597 537 393 268
72 15
EcoO109I rG'GnC_Cy
Cuts at: 3331 3511 3331
Size: 180 6376
EcoRI G'AATT_C
Cuts at: 3865 4243 3865
Size: 378 6178
EcoRV GAT'ATC
Cuts at: 2506 2506
Size: 6556
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 2749 5703 1678
Size: 1071 2954 2531
Fragments arranged by size:
2954 2531 1071
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3596 3628 5197 5229 5271
Size: 32 135 32 2024 32 1569 32 42
Cuts at: 5271 5303 1373
Size: 32 2626
Fragments arranged by size:
2626 2024 1569 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3667 3900 4146 669
Size: 988 281 1729 233 246 3079
Fragments arranged by size:
3079 1729 988 281 246 233
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6556
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 2251 3881 2251
Size: 1630 4926
MfeI C'AATT_G
Cuts at: 4155 4155
Size: 6556
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3594 5670 5854 1305
Size: 2289 2076 184 2007
Fragments arranged by size:
2289 2076 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 2957 3056 3851 10
Size: 54 1425 343 198 927 99 795 2715
Fragments arranged by size:
2715 1425 927 795 343 198 99 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 2786 3149 4514 4873 5032 518
Size: 648 1425 195 363 1365 359 159 2042
Fragments arranged by size:
2042 1425 1365 648 363 359 195 159
MspA1I CmG'CkG
Cuts at: 1606 2203 2310 3163 3610 3662 3679 3805 3972
Size: 597 107 853 447 52 17 126 167
Cuts at: 3972 4649 5797 6042 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 853 677 597 447 245 167
126 107 52 17
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 6556
NcoI C'CATG_G
Cuts at: 513 1065 3735 513
Size: 552 2670 3334
Fragments arranged by size:
3334 2670 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6556
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 6556
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6556
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 2596 3559 3694 3914 5459 1132
Size: 97 1367 963 135 220 1545 2229
Fragments arranged by size:
2229 1545 1367 963 220 135 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6556
PciI A'CATG_T
Cuts at: 2592 5455 2592
Size: 2863 3693
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2920 3063 3502 1108
Size: 1812 143 439 4162
Fragments arranged by size:
4162 1812 439 143
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PmlI CAC'GTG
Cuts at: 3205 3304 3205
Size: 99 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2555 2587 4625 4657 6138 6170 2555
Size: 32 2038 32 1481 32 2941
Fragments arranged by size:
2941 2038 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3331 3511 3331
Size: 180 6376
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6556
PsiI TTA'TAA
Cuts at: 4126 4126
Size: 6556
PspXI vC'TCGA_Gb
Cuts at: 3758 3758
Size: 6556
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 6524
PstI C_TGCA'G
Cuts at: 830 2102 3525 3908 830
Size: 1272 1423 383 3478
Fragments arranged by size:
3478 1423 1272 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
PvuII CAG'CTG
Cuts at: 2203 3163 3610 3679 3805 2203
Size: 960 447 69 126 4954
Fragments arranged by size:
4954 960 447 126 69
SacII CC_GC'GG
Cuts at: 3663 3663
Size: 6556
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SapI GCTCTTCn'nnn_
Cuts at: 3696 3696
Size: 6556
SbfI CC_TGCA'GG
Cuts at: 3908 3908
Size: 6556
ScaI AGT'ACT
Cuts at: 2228 4804 6516 2228
Size: 2576 1712 2268
Fragments arranged by size:
2576 2268 1712
SexAI A'CCwGG_T
Cuts at: 3495 3495
Size: 6556
SfcI C'TryA_G
Cuts at: 826 1045 2098 3521 3904 5039 5720 5911 826
Size: 219 1053 1423 383 1135 681 191 1471
Fragments arranged by size:
1471 1423 1135 1053 681 383 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3371 3371
Size: 6556
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 2318 1700
Size: 618 5938
SmaI CCC'GGG
Cuts at: 1872 3000 3377 3883 1872
Size: 1128 377 506 4545
Fragments arranged by size:
4545 1128 506 377
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2387 2464 3758 4660 5561 5823
Size: 197 926 444 77 1294 902 901 262
Cuts at: 5823 820
Size: 1553
Fragments arranged by size:
1553 1294 926 902 901 444 262 197
77
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 3559 3914 1132
Size: 2427 355 3774
Fragments arranged by size:
3774 2427 355
SspI AAT'ATT
Cuts at: 5 52 4480 5
Size: 47 4428 2081
Fragments arranged by size:
4428 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6556
StyI C'CwwG_G
Cuts at: 513 1065 3231 3735 3995 513
Size: 552 2166 504 260 3074
Fragments arranged by size:
3074 2166 552 504 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3055 3119 3580 4583 4600 4753 4938
Size: 1255 1475 64 461 1003 17 153 185
Cuts at: 4938 5383 325
Size: 445 1498
Fragments arranged by size:
1498 1475 1255 1003 461 445 185 153
64 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2226 2256 4802
Size: 274 80 33 51 1443 249 30 2546
Cuts at: 4802 6514 96
Size: 1712 138
Fragments arranged by size:
2546 1712 1443 274 249 138 80 51
33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2901 2933 6516 6548 2901
Size: 32 3583 32 2909
Fragments arranged by size:
3583 2909 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6556
XbaI T'CTAG_A
Cuts at: 3892 3892
Size: 6556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2717 2717
Size: 6556
XhoI C'TCGA_G
Cuts at: 1943 3758 1943
Size: 1815 4741
XmaI C'CCGG_G
Cuts at: 1870 2998 3375 3881 1870
Size: 1128 377 506 4545
Fragments arranged by size:
4545 1128 506 377
XmnI GAAnn'nnTTC
Cuts at: 2570 2615 2849 4685 2570
Size: 45 234 1836 4441
Fragments arranged by size:
4441 1836 234 45
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PspOMI
RsrII SacI SanDI SfoI SrfI SwaI