Restriction map for
pFN21AE2050
(Circular) (Six-base) MAPSORT of: pFN21AE2050.seq Check: 8713 from: 1 to: 6718
pFN21AE2050 6718 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4528 278
Size: 53 83 186 936 2992 2468
Fragments arranged by size:
2992 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4061 1937
Size: 2124 4594
Acc65I G'GTAC_C
Cuts at: 4039 4039
Size: 6718
AclI AA'CG_TT
Cuts at: 1563 3751 4845 5218 1563
Size: 2188 1094 373 3063
Fragments arranged by size:
3063 2188 1094 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 2631 2702 3071 4781 6165 6433 1632
Size: 725 274 71 369 1710 1384 268 1917
Fragments arranged by size:
1917 1710 1384 725 369 274 268 71
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6521
AflIII A'CryG_T
Cuts at: 1203 5617 6594 1203
Size: 4414 977 1327
Fragments arranged by size:
4414 1327 977
AgeI A'CCGG_T
Cuts at: 2061 5549 2061
Size: 3488 3230
AhdI GACnn_n'nnGTC
Cuts at: 5447 5447
Size: 6718
AleI CACnn'nnGTG
Cuts at: 1166 2222 1166
Size: 1056 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6718
AlwNI CAG_nnn'CTG
Cuts at: 2039 3908 4461 6033 2039
Size: 1869 553 1572 2724
Fragments arranged by size:
2724 1869 1572 553
ApaLI G'TGCA_C
Cuts at: 2556 4775 5931 2556
Size: 2219 1156 3343
Fragments arranged by size:
3343 2219 1156
ApoI r'AATT_y
Cuts at: 1493 2924 3092 3153 3571 4027 4253 4405 6514
Size: 1431 168 61 418 456 226 152 2109
Cuts at: 6514 1493
Size: 1697
Fragments arranged by size:
2109 1697 1431 456 418 226 168 152
61
AseI AT'TA_AT
Cuts at: 160 1032 5272 160
Size: 872 4240 1606
Fragments arranged by size:
4240 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 4043 1870
Size: 73 1065 1035 4545
Fragments arranged by size:
4545 1065 1035 73
BamHI G'GATC_C
Cuts at: 4048 4415 4048
Size: 367 6351
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4039 5494 618
Size: 325 217 627 2252 1455 1842
Fragments arranged by size:
2252 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3961 4037 1391
Size: 2570 76 4072
Fragments arranged by size:
4072 2570 76
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2306 2646 2667 3280 928
Size: 971 407 340 21 613 4366
Fragments arranged by size:
4366 971 613 407 340 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4909 4943 1903
Size: 34 2972 34 3678
Fragments arranged by size:
3678 2972 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4610 5820 4610
Size: 1210 5508
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6718
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2076 5329 136
Size: 107 122 71 631 605 404 3253 1525
Fragments arranged by size:
3253 1525 631 605 404 122 107 71
BglII A'GATC_T
Cuts at: 2404 2614 6713 2404
Size: 210 4099 2409
Fragments arranged by size:
4099 2409 210
BlpI GC'TnA_GC
Cuts at: 2389 4135 2389
Size: 1746 4972
Bme1580I G_kGCm'C
Cuts at: 1790 2560 4779 5935 1790
Size: 770 2219 1156 2573
Fragments arranged by size:
2573 2219 1156 770
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6718
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4449 5407 442
Size: 416 53 83 399 24 3032 958 1753
Fragments arranged by size:
3032 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6718
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3639 3945 5378 1239
Size: 2400 306 1433 2579
Fragments arranged by size:
2579 2400 1433 306
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2081 3144 4843 5708 6006 2081
Size: 1063 1699 865 298 2793
Fragments arranged by size:
2793 1699 1063 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5381 882
Size: 617 3882 2219
Fragments arranged by size:
3882 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4509 493
Size: 711 3305 2702
Fragments arranged by size:
3305 2702 711
BsaBI GATnn'nnATC
Cuts at: 3105 6712 3105
Size: 3607 3111
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4525 4907 5542 275
Size: 53 83 186 936 2992 382 635 1451
Fragments arranged by size:
2992 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2061 5151 5549 5823 5970 6381 2061
Size: 3090 398 274 147 411 2398
Fragments arranged by size:
3090 2398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 1178
Size: 30 165 30 825 30 5638
Fragments arranged by size:
5638 825 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2453 2747 3218 1186
Size: 1267 294 471 4686
Fragments arranged by size:
4686 1267 471 294
BseYI C'CCAG_C
Cuts at: 3042 4014 5921 6417 3042
Size: 972 1907 496 3343
Fragments arranged by size:
3343 1907 972 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6145
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4168 4929 5957 1540
Size: 275 186 2167 761 1028 2301
Fragments arranged by size:
2301 2167 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2560 4779 4864 5935 2560
Size: 2219 85 1071 3343
Fragments arranged by size:
3343 2219 1071 85
BsmI GAATG_Cn'
Cuts at: 2296 2390 4229 4322 2296
Size: 94 1839 93 4692
Fragments arranged by size:
4692 1839 94 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2560 3961 4037 4779 4864 5935 1391
Size: 399 770 1401 76 742 85 1071 2174
Fragments arranged by size:
2174 1401 1071 770 742 399 85 76
BspEI T'CCGG_A
Cuts at: 6381 6381
Size: 6718
BspHI T'CATG_A
Cuts at: 4607 6337 4607
Size: 1730 4988
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2047 3958 4073 844
Size: 1203 1911 115 3489
Fragments arranged by size:
3489 1911 1203 115
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 4605 1394
Size: 429 190 2592 3507
Fragments arranged by size:
3507 2592 429 190
BsrDI GCAATG_nn'
Cuts at: 65 1248 5213 5387 65
Size: 1183 3965 174 1396
Fragments arranged by size:
3965 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2061 5362 5549 1700
Size: 110 251 3301 187 2869
Fragments arranged by size:
3301 2869 251 187 110
BsrGI T'GTAC_A
Cuts at: 96 2949 96
Size: 2853 3865
BssHII G'CGCG_C
Cuts at: 1925 2089 1925
Size: 164 6554
BssSI C'ACGA_G
Cuts at: 4778 5790 4778
Size: 1012 5706
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2468 794
Size: 1674 5044
BstBI TT'CG_AA
Cuts at: 5522 5522
Size: 6718
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6718
BstXI CCAn_nnnn'nTGG
Cuts at: 3732 3732
Size: 6718
BstYI r'GATC_y
Cuts at: 1972 2404 2614 3487 3721 4048 4415 4800 4817
Size: 432 210 873 234 327 367 385 17
Cuts at: 4817 6269 6355 6367 6713 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 873 432 385 367 346 327
234 210 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3138 513
Size: 552 939 1134 4093
Fragments arranged by size:
4093 1134 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5979
Fragments arranged by size:
5979 683 56
BtsI GCAGTG_nn'
Cuts at: 2400 2547 3898 4234 5028 5048 2400
Size: 147 1351 336 794 20 4070
Fragments arranged by size:
4070 1351 794 336 147 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6718
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2763 2798 3404 3439 529
Size: 35 2199 35 606 35 3808
Fragments arranged by size:
3808 2199 606 35 35 35
DraI TTT'AAA
Cuts at: 4022 4022
Size: 6718
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2837 5725 809
Size: 507 1521 2888 1802
Fragments arranged by size:
2888 1802 1521 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2068 2679 4165
Size: 54 1425 50 275 18 238 611 1486
Cuts at: 4165 5054 6683 8
Size: 889 1629 43
Fragments arranged by size:
1629 1486 1425 889 611 275 238 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4165 1537
Size: 275 2353 4090
Fragments arranged by size:
4090 2353 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4648 1353
Size: 3295 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3403 5305 5679 5825 387
Size: 1064 138 1814 1902 374 146 1280
Fragments arranged by size:
1902 1814 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2357 2631 2702 3071 3639 3945 4781
Size: 393 725 274 71 369 568 306 836
Cuts at: 4781 5378 6165 6433 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 836 787 725 597 568 393 369
306 274 268 71
EcoO109I rG'GnC_Cy
Cuts at: 3807 3807
Size: 6718
EcoRI G'AATT_C
Cuts at: 4027 4405 4027
Size: 378 6340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2957 2957
Size: 6718
FspI TGC'GCA
Cuts at: 5224 5224
Size: 6718
HaeII r_GCGC'y
Cuts at: 1678 5865 1678
Size: 4187 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2394 2426 3524 3556 5359
Size: 32 135 32 822 32 1098 32 1803
Cuts at: 5359 5391 5433 5465 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1803 1098 822 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3629 4062 4308 669
Size: 988 281 1691 433 246 3079
Fragments arranged by size:
3079 1691 988 433 281 246
HindIII A'AGCT_T
Cuts at: 748 2193 2894 2934 748
Size: 1445 701 40 4532
Fragments arranged by size:
4532 1445 701 40
HpaI GTT'AAC
Cuts at: 4308 4308
Size: 6718
KpnI G_GTAC'C
Cuts at: 4043 4043
Size: 6718
MfeI C'AATT_G
Cuts at: 2482 4317 2482
Size: 1835 4883
MluI A'CGCG_T
Cuts at: 6594 6594
Size: 6718
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5832 6016 1305
Size: 4527 184 2007
Fragments arranged by size:
4527 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2070 2681 10
Size: 54 1425 343 238 611 4047
Fragments arranged by size:
4047 1425 611 343 238 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 4676 5035 5194 518
Size: 648 1056 2454 359 159 2042
Fragments arranged by size:
2454 2042 1056 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2578 3122 3421 4134 4811 5959 6204
Size: 416 556 544 299 713 677 1148 245
Cuts at: 6204 1606
Size: 2120
Fragments arranged by size:
2120 1148 713 677 556 544 416 299
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3138 513
Size: 552 939 1134 4093
Fragments arranged by size:
4093 1134 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6718
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6718
NotI GC'GGCC_GC
Cuts at: 4165 4165
Size: 6718
NspI r_CATG'y
Cuts at: 1132 1229 2556 3183 4076 5621 1132
Size: 97 1327 627 893 1545 2229
Fragments arranged by size:
2229 1545 1327 893 627 97
PasI CC'CwG_GG
Cuts at: 1348 3811 1348
Size: 2463 4255
PciI A'CATG_T
Cuts at: 5617 5617
Size: 6718
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3887 1108
Size: 2779 3939
PmeI GTTT'AAAC
Cuts at: 4022 4022
Size: 6718
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4787 4819 6300 6332 4787
Size: 32 1481 32 5173
Fragments arranged by size:
5173 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3807 3807
Size: 6718
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6718
PsiI TTA'TAA
Cuts at: 4288 4288
Size: 6718
PstI C_TGCA'G
Cuts at: 830 2715 3826 3955 4070 830
Size: 1885 1111 129 115 3478
Fragments arranged by size:
3478 1885 1111 129 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6718
PvuII CAG'CTG
Cuts at: 2578 3122 3421 2578
Size: 544 299 5875
Fragments arranged by size:
5875 544 299
SalI G'TCGA_C
Cuts at: 1936 4060 1936
Size: 2124 4594
SanDI GG'GwC_CC
Cuts at: 3807 3807
Size: 6718
SbfI CC_TGCA'GG
Cuts at: 3955 4070 3955
Size: 115 6603
ScaI AGT'ACT
Cuts at: 4966 6678 4966
Size: 1712 5006
SfcI C'TryA_G
Cuts at: 826 1045 2117 2711 2919 3822 3951 4066 5201
Size: 219 1072 594 208 903 129 115 1135
Cuts at: 5201 5882 6073 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1072 903 681 594 219 208
191 129 115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6718
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6718
SmaI CCC'GGG
Cuts at: 1872 4045 1872
Size: 2173 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2096 3159 4822 5723 5985 820
Size: 197 926 153 1063 1663 901 262 1553
Fragments arranged by size:
1663 1553 1063 926 901 262 197 153
SnaBI TAC'GTA
Cuts at: 493 4509 493
Size: 4016 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6718
SphI G_CATG'C
Cuts at: 1132 4076 1132
Size: 2944 3774
SspI AAT'ATT
Cuts at: 5 52 3169 3189 4642 5
Size: 47 3117 20 1453 2081
Fragments arranged by size:
3117 2081 1453 47 20
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6718
StyI C'CwwG_G
Cuts at: 513 1065 2004 2569 3138 4157 513
Size: 552 939 565 569 1019 3074
Fragments arranged by size:
3074 1019 939 569 565 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3886 4745 4762 4915 5100 5545 325
Size: 1255 2306 859 17 153 185 445 1498
Fragments arranged by size:
2306 1498 1255 859 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 2949 4964
Size: 274 80 33 51 1443 291 681 2015
Cuts at: 4964 6676 96
Size: 1712 138
Fragments arranged by size:
2015 1712 1443 681 291 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3285 3317 3589 3621 6678 6710 3285
Size: 32 272 32 3057 32 3293
Fragments arranged by size:
3293 3057 272 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6718
XbaI T'CTAG_A
Cuts at: 4054 4054
Size: 6718
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6718
XmaI C'CCGG_G
Cuts at: 1870 4043 1870
Size: 2173 4545
XmnI GAAnn'nnTTC
Cuts at: 4847 4847
Size: 6718
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4526 276
Size: 53 83 186 936 2992 2468
Fragments arranged by size:
2992 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI