Restriction map for
pFN21AE2046
(Circular) (Six-base) MAPSORT of: pFN21AE2046.seq Check: 5923 from: 1 to: 6697
pFN21AE2046 6697 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4507 278
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4040 1937
Size: 2103 4594
Acc65I G'GTAC_C
Cuts at: 4018 4018
Size: 6697
AclI AA'CG_TT
Cuts at: 1563 4824 5197 1563
Size: 3261 373 3063
Fragments arranged by size:
3261 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3145 4760 6144 6412 1632
Size: 1513 1615 1384 268 1917
Fragments arranged by size:
1917 1615 1513 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2059 3797 820
Size: 197 1042 1738 3720
Fragments arranged by size:
3720 1738 1042 197
AflIII A'CryG_T
Cuts at: 1203 5596 6573 1203
Size: 4393 977 1327
Fragments arranged by size:
4393 1327 977
AgeI A'CCGG_T
Cuts at: 5528 5528
Size: 6697
AhdI GACnn_n'nnGTC
Cuts at: 5426 5426
Size: 6697
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2828 1184
Size: 1644 5053
AlwNI CAG_nnn'CTG
Cuts at: 2137 4440 6012 2137
Size: 2303 1572 2822
Fragments arranged by size:
2822 2303 1572
ApaLI G'TGCA_C
Cuts at: 4754 5910 4754
Size: 1156 5541
ApoI r'AATT_y
Cuts at: 1493 2356 2706 2897 3942 4006 4232 4384 6493
Size: 863 350 191 1045 64 226 152 2109
Cuts at: 6493 1493
Size: 1697
Fragments arranged by size:
2109 1697 1045 863 350 226 191 152
64
AseI AT'TA_AT
Cuts at: 160 1032 3684 5251 160
Size: 872 2652 1567 1606
Fragments arranged by size:
2652 1606 1567 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
AvaI C'yCGr_G
Cuts at: 1870 1943 4022 1870
Size: 73 2079 4545
Fragments arranged by size:
4545 2079 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3597 3630 3597
Size: 33 6664
BamHI G'GATC_C
Cuts at: 3848 4027 4394 3848
Size: 179 367 6151
Fragments arranged by size:
6151 367 179
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3792 3914 4018 5473 618
Size: 325 217 627 2005 122 104 1455 1842
Fragments arranged by size:
2005 1842 1455 627 325 217 122 104
BanII G_rGCy'C
Cuts at: 1391 4016 1391
Size: 2625 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2594 928
Size: 971 695 5031
Fragments arranged by size:
5031 971 695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4888 4922 1903
Size: 34 2951 34 3678
Fragments arranged by size:
3678 2951 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2128 4589 5799 2128
Size: 2461 1210 3026
Fragments arranged by size:
3026 2461 1210
BclI T'GATC_A
Cuts at: 1548 2668 1548
Size: 1120 5577
BfrBI ATG'CAT
Cuts at: 3365 3365
Size: 6697
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5308 136
Size: 107 122 71 631 605 3636 1525
Fragments arranged by size:
3636 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6692 6692
Size: 6697
BlpI GC'TnA_GC
Cuts at: 4114 4114
Size: 6697
Bme1580I G_kGCm'C
Cuts at: 1790 3159 4758 5914 1790
Size: 1369 1599 1156 2573
Fragments arranged by size:
2573 1599 1369 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6697
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4428 5386 442
Size: 416 53 83 399 24 3011 958 1753
Fragments arranged by size:
3011 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3134 5357 1239
Size: 1895 2223 2579
Fragments arranged by size:
2579 2223 1895
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2379 3756 4822 5687 5985 2379
Size: 1377 1066 865 298 3091
Fragments arranged by size:
3091 1377 1066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3478 5360 882
Size: 617 1979 1882 2219
Fragments arranged by size:
2219 1979 1882 617
BsaAI yAC'GTr
Cuts at: 493 1204 2889 4488 493
Size: 711 1685 1599 2702
Fragments arranged by size:
2702 1685 1599 711
BsaBI GATnn'nnATC
Cuts at: 6691 6691
Size: 6697
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4504 4886 5521 275
Size: 53 83 186 936 2971 382 635 1451
Fragments arranged by size:
2971 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5130 5528 5802 5949 6360 5130
Size: 398 274 147 411 5467
Fragments arranged by size:
5467 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2621 2651 3107 3137 1178
Size: 30 165 30 1218 30 456 30 4738
Fragments arranged by size:
4738 1218 456 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 2462 3605 1186
Size: 1273 3 1143 4278
Fragments arranged by size:
4278 1273 1143 3
BseYI C'CCAG_C
Cuts at: 3152 5900 6396 3152
Size: 2748 496 3453
Fragments arranged by size:
3453 2748 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 2911 3635 1163
Size: 573 657 518 724 4225
Fragments arranged by size:
4225 724 657 573 518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4147 4908 5936 1540
Size: 275 186 2146 761 1028 2301
Fragments arranged by size:
2301 2146 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4758 4843 5914 4758
Size: 85 1071 5541
Fragments arranged by size:
5541 1071 85
BsmI GAATG_Cn'
Cuts at: 4208 4301 4208
Size: 93 6604
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3159 4016 4758 4843 5914 1391
Size: 399 1369 857 742 85 1071 2174
Fragments arranged by size:
2174 1369 1071 857 742 399 85
BspEI T'CCGG_A
Cuts at: 6360 6360
Size: 6697
BspHI T'CATG_A
Cuts at: 4586 6316 4586
Size: 1730 4967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4052 844
Size: 3208 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2714 4584 1394
Size: 429 891 1870 3507
Fragments arranged by size:
3507 1870 891 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2805 3411 3525 5192 5366 65
Size: 1183 1557 606 114 1667 174 1396
Fragments arranged by size:
1667 1557 1396 1183 606 174 114
BsrFI r'CCGG_y
Cuts at: 1700 1810 5341 5528 1700
Size: 110 3531 187 2869
Fragments arranged by size:
3531 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6697
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6697
BssSI C'ACGA_G
Cuts at: 4757 5769 4757
Size: 1012 5685
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2089 794
Size: 1295 5402
BstBI TT'CG_AA
Cuts at: 5501 5501
Size: 6697
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6697
BstYI r'GATC_y
Cuts at: 1972 3848 4027 4394 4779 4796 6248 6334 6346
Size: 1876 179 367 385 17 1452 86 12
Cuts at: 6346 6692 1972
Size: 346 1977
Fragments arranged by size:
1977 1876 1452 385 367 346 179 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2878 513
Size: 552 939 874 4332
Fragments arranged by size:
4332 939 874 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5958
Fragments arranged by size:
5958 683 56
BtsI GCAGTG_nn'
Cuts at: 2381 2817 3391 4213 5007 5027 2381
Size: 436 574 822 794 20 4051
Fragments arranged by size:
4051 822 794 574 436 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6697
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6662
DraI TTT'AAA
Cuts at: 4001 4001
Size: 6697
DraIII CAC_nnn'GTG
Cuts at: 3196 3196
Size: 6697
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5704 809
Size: 507 4388 1802
Fragments arranged by size:
4388 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3185 4144 5033
Size: 54 1425 50 275 18 1355 959 889
Cuts at: 5033 6662 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1355 959 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4144 1537
Size: 275 2332 4090
Fragments arranged by size:
4090 2332 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2521 3138 4627 1353
Size: 1168 617 1489 3423
Fragments arranged by size:
3423 1489 1168 617
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5284 5658 5804 387
Size: 1064 138 3695 374 146 1280
Fragments arranged by size:
3695 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3134 3145 4760 5357 6144 6412 1239
Size: 393 1502 11 1615 597 787 268 1524
Fragments arranged by size:
1615 1524 1502 787 597 393 268 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2700 3813 2700
Size: 1113 5584
EcoO109I rG'GnC_Cy
Cuts at: 3856 3856
Size: 6697
EcoRI G'AATT_C
Cuts at: 2706 3942 4006 4384 2706
Size: 1236 64 378 5019
Fragments arranged by size:
5019 1236 378 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2074 2074
Size: 6697
FspI TGC'GCA
Cuts at: 5203 5203
Size: 6697
HaeII r_GCGC'y
Cuts at: 1678 5844 1678
Size: 4166 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2325 2357 2668
Size: 32 135 32 530 32 191 32 311
Cuts at: 2668 2700 2740 2772 3650 3682 5338 5370 5412
Size: 32 40 32 878 32 1656 32 42
Cuts at: 5412 5444 1373
Size: 32 2626
Fragments arranged by size:
2626 1656 878 530 311 191 135 42
40 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3751 4041 4287 669
Size: 988 281 1813 290 246 3079
Fragments arranged by size:
3079 1813 988 290 281 246
HindIII A'AGCT_T
Cuts at: 748 2620 748
Size: 1872 4825
HpaI GTT'AAC
Cuts at: 3751 4287 3751
Size: 536 6161
KpnI G_GTAC'C
Cuts at: 4022 4022
Size: 6697
MfeI C'AATT_G
Cuts at: 4296 4296
Size: 6697
MluI A'CGCG_T
Cuts at: 6573 6573
Size: 6697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2156 2813 2935 5811 5995 1305
Size: 851 657 122 2876 184 2007
Fragments arranged by size:
2876 2007 851 657 184 122
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4875
Fragments arranged by size:
4875 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2799 2810 4655 5014 5173 518
Size: 648 1633 11 1845 359 159 2042
Fragments arranged by size:
2042 1845 1633 648 359 159 11
MspA1I CmG'CkG
Cuts at: 1606 2185 4113 4790 5938 6183 1606
Size: 579 1928 677 1148 245 2120
Fragments arranged by size:
2120 1928 1148 677 579 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2878 513
Size: 552 939 874 4332
Fragments arranged by size:
4332 939 874 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6697
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6697
NotI GC'GGCC_GC
Cuts at: 4144 4144
Size: 6697
NsiI A_TGCA'T
Cuts at: 3367 3367
Size: 6697
NspI r_CATG'y
Cuts at: 1132 1229 4055 5600 1132
Size: 97 2826 1545 2229
Fragments arranged by size:
2826 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6697
PciI A'CATG_T
Cuts at: 5596 5596
Size: 6697
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2594 1108
Size: 1486 5211
PmeI GTTT'AAAC
Cuts at: 4001 4001
Size: 6697
PmlI CAC'GTG
Cuts at: 2889 2889
Size: 6697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2039 2071 4766 4798 6279 6311 2039
Size: 32 2695 32 1481 32 2425
Fragments arranged by size:
2695 2425 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3856 3856
Size: 6697
PshAI GACnn'nnGTC
Cuts at: 1613 3861 1613
Size: 2248 4449
PsiI TTA'TAA
Cuts at: 4267 4267
Size: 6697
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2980 3012 2980
Size: 32 6665
PstI C_TGCA'G
Cuts at: 830 3923 4049 830
Size: 3093 126 3478
Fragments arranged by size:
3478 3093 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6697
PvuII CAG'CTG
Cuts at: 2185 2185
Size: 6697
SalI G'TCGA_C
Cuts at: 1936 4039 1936
Size: 2103 4594
SbfI CC_TGCA'GG
Cuts at: 3923 4049 3923
Size: 126 6571
ScaI AGT'ACT
Cuts at: 4945 6657 4945
Size: 1712 4985
SexAI A'CCwGG_T
Cuts at: 3487 3487
Size: 6697
SfcI C'TryA_G
Cuts at: 826 1045 2271 3031 3919 4045 5180 5861 6052
Size: 219 1226 760 888 126 1135 681 191
Cuts at: 6052 826
Size: 1471
Fragments arranged by size:
1471 1226 1135 888 760 681 219 191
126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6697
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6697
SmaI CCC'GGG
Cuts at: 1872 4024 1872
Size: 2152 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2059 2394 3771 3797 4801 5702
Size: 197 926 116 335 1377 26 1004 901
Cuts at: 5702 5964 820
Size: 262 1553
Fragments arranged by size:
1553 1377 1004 926 901 335 262 197
116 26
SnaBI TAC'GTA
Cuts at: 493 4488 493
Size: 3995 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6697
SphI G_CATG'C
Cuts at: 1132 4055 1132
Size: 2923 3774
SspI AAT'ATT
Cuts at: 5 52 4621 5
Size: 47 4569 2081
Fragments arranged by size:
4569 2081 47
StuI AGG'CCT
Cuts at: 1517 2392 1517
Size: 875 5822
StyI C'CwwG_G
Cuts at: 513 1065 2004 2878 3182 3577 4136 513
Size: 552 939 874 304 395 559 3074
Fragments arranged by size:
3074 939 874 559 552 395 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2811 4724 4741 4894 5079 5524 325
Size: 1255 1231 1913 17 153 185 445 1498
Fragments arranged by size:
1913 1498 1255 1231 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3778 4943 6655
Size: 274 80 33 51 1443 1801 1165 1712
Cuts at: 6655 96
Size: 138
Fragments arranged by size:
1801 1712 1443 1165 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3916 3948 6657 6689 3916
Size: 32 2709 32 3924
Fragments arranged by size:
3924 2709 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6697
XbaI T'CTAG_A
Cuts at: 2861 4033 2861
Size: 1172 5525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2134 2155 2134
Size: 21 6676
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6697
XmaI C'CCGG_G
Cuts at: 1870 4022 1870
Size: 2152 4545
XmnI GAAnn'nnTTC
Cuts at: 2054 3471 4826 2054
Size: 1417 1355 3925
Fragments arranged by size:
3925 1417 1355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4505 276
Size: 53 83 186 936 2971 2468
Fragments arranged by size:
2971 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PspOMI PspXI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI