Restriction map for
pFN21AE2045
(Circular) (Six-base) MAPSORT of: pFN21AE2045.seq Check: 2315 from: 1 to: 6694
pFN21AE2045 6694 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4504 278
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4037 1937
Size: 2100 4594
Acc65I G'GTAC_C
Cuts at: 4015 4015
Size: 6694
AclI AA'CG_TT
Cuts at: 1563 4821 5194 1563
Size: 3258 373 3063
Fragments arranged by size:
3258 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2558 2768 2792 2933 3980 4757 6141 6409
Size: 926 210 24 141 1047 777 1384 268
Cuts at: 6409 1632
Size: 1917
Fragments arranged by size:
1917 1384 1047 926 777 268 210 141
24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6497
AflIII A'CryG_T
Cuts at: 1203 2226 2370 2569 5593 6570 1203
Size: 1023 144 199 3024 977 1327
Fragments arranged by size:
3024 1327 1023 977 199 144
AgeI A'CCGG_T
Cuts at: 5525 5525
Size: 6694
AhdI GACnn_n'nnGTC
Cuts at: 5423 5423
Size: 6694
AleI CACnn'nnGTG
Cuts at: 1166 3515 1166
Size: 2349 4345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2267 1184
Size: 1083 5611
AlwNI CAG_nnn'CTG
Cuts at: 4437 6009 4437
Size: 1572 5122
ApaI G_GGCC'C
Cuts at: 2261 3214 2261
Size: 953 5741
ApaLI G'TGCA_C
Cuts at: 3610 4751 5907 3610
Size: 1141 1156 4397
Fragments arranged by size:
4397 1156 1141
ApoI r'AATT_y
Cuts at: 1493 2853 3049 3240 4003 4229 4381 6490 1493
Size: 1360 196 191 763 226 152 2109 1697
Fragments arranged by size:
2109 1697 1360 763 226 196 191 152
AseI AT'TA_AT
Cuts at: 160 1032 5248 160
Size: 872 4216 1606
Fragments arranged by size:
4216 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
AvaI C'yCGr_G
Cuts at: 1870 1943 2330 2767 3207 4019 1870
Size: 73 387 437 440 812 4545
Fragments arranged by size:
4545 812 440 437 387 73
BamHI G'GATC_C
Cuts at: 4024 4391 4024
Size: 367 6327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2336 2708 3135 3817 3828
Size: 325 217 627 549 372 427 682 11
Cuts at: 3828 4015 5470 618
Size: 187 1455 1842
Fragments arranged by size:
1842 1455 682 627 549 427 372 325
217 187 11
BanII G_rGCy'C
Cuts at: 1391 2181 2261 2551 2591 2925 3214 4013 1391
Size: 790 80 290 40 334 289 799 4072
Fragments arranged by size:
4072 799 790 334 290 289 80 40
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2613 3595 3761 928
Size: 971 714 982 166 3861
Fragments arranged by size:
3861 982 971 714 166
BbvCI CC'TCA_GC
Cuts at: 3108 3108
Size: 6694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3779 3813 4885 4919 1903
Size: 34 1842 34 1072 34 3678
Fragments arranged by size:
3678 1842 1072 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4586 5796 4586
Size: 1210 5484
BclI T'GATC_A
Cuts at: 1548 2152 2614 1548
Size: 604 462 5628
Fragments arranged by size:
5628 604 462
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 3826 5305
Size: 107 122 71 631 605 364 1790 1479
Cuts at: 5305 136
Size: 1525
Fragments arranged by size:
1790 1525 1479 631 605 364 122 107
71
BglII A'GATC_T
Cuts at: 2415 2619 6689 2415
Size: 204 4070 2420
Fragments arranged by size:
4070 2420 204
BlpI GC'TnA_GC
Cuts at: 3306 4111 3306
Size: 805 5889
Bme1580I G_kGCm'C
Cuts at: 1790 2261 2339 2644 3214 3614 3831 4755 5911
Size: 471 78 305 570 400 217 924 1156
Cuts at: 5911 1790
Size: 2573
Fragments arranged by size:
2573 1156 924 570 471 400 305 217
78
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6694
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4425 5383 442
Size: 416 53 83 399 24 3008 958 1753
Fragments arranged by size:
3008 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2283 2573 2734 2816 5354 1239
Size: 890 154 290 161 82 2538 2579
Fragments arranged by size:
2579 2538 890 290 161 154 82
Bpu10I CC'TnA_GC
Cuts at: 2321 2585 3108 3483 2321
Size: 264 523 375 5532
Fragments arranged by size:
5532 523 375 264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4819 5684 5982 4819
Size: 865 298 5531
Fragments arranged by size:
5531 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5357 882
Size: 617 3858 2219
Fragments arranged by size:
3858 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3609 4485 493
Size: 711 2405 876 2702
Fragments arranged by size:
2702 2405 876 711
BsaBI GATnn'nnATC
Cuts at: 6688 6688
Size: 6694
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4501 4883 5518 275
Size: 53 83 186 936 2968 382 635 1451
Fragments arranged by size:
2968 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5127 5525 5799 5946 6357 5127
Size: 398 274 147 411 5464
Fragments arranged by size:
5464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2132 2162 3273 3303 1178
Size: 30 165 30 729 30 1111 30 4569
Fragments arranged by size:
4569 1111 729 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2156 2159 2522 2889 1186
Size: 967 3 3 363 367 4991
Fragments arranged by size:
4991 967 367 363 3 3
BseYI C'CCAG_C
Cuts at: 2298 2424 2972 3361 3618 3965 5897 6393 2298
Size: 126 548 389 257 347 1932 496 2599
Fragments arranged by size:
2599 1932 548 496 389 347 257 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2090 2273 2324 3050 3134 1163
Size: 573 354 183 51 726 84 4723
Fragments arranged by size:
4723 726 573 354 183 84 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2126 2242 4144 4905 5933 1540
Size: 275 186 125 116 1902 761 1028 2301
Fragments arranged by size:
2301 1902 1028 761 275 186 125 116
BsiHKAI G_wGCw'C
Cuts at: 2591 3263 3379 3614 4755 4840 5911 2591
Size: 672 116 235 1141 85 1071 3374
Fragments arranged by size:
3374 1141 1071 672 235 116 85
BsmI GAATG_Cn'
Cuts at: 4205 4298 4205
Size: 93 6601
BsmBI CGTCTCn'nnnn_
Cuts at: 2722 2722
Size: 6694
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2261 2339 2551 2591 2644 2925
Size: 399 391 80 78 212 40 53 281
Cuts at: 2925 3214 3263 3379 3614 3831 4013 4755 4840
Size: 289 49 116 235 217 182 742 85
Cuts at: 4840 5911 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 391 289 281 235
217 212 182 116 85 80 78 53
49 40
BspEI T'CCGG_A
Cuts at: 6357 6357
Size: 6694
BspHI T'CATG_A
Cuts at: 2155 4583 6313 2155
Size: 2428 1730 2536
Fragments arranged by size:
2536 2428 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4049 844
Size: 3205 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4581 1394
Size: 429 2758 3507
Fragments arranged by size:
3507 2758 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3414 5189 5363 65
Size: 1183 2166 1775 174 1396
Fragments arranged by size:
2166 1775 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2125 5338 5525 1700
Size: 110 315 3213 187 2869
Fragments arranged by size:
3213 2869 315 187 110
BsrGI T'GTAC_A
Cuts at: 96 3280 96
Size: 3184 3510
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6694
BssSI C'ACGA_G
Cuts at: 3068 4754 5766 3068
Size: 1686 1012 3996
Fragments arranged by size:
3996 1686 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6694
BstBI TT'CG_AA
Cuts at: 5498 5498
Size: 6694
BstEII G'GTnAC_C
Cuts at: 1184 2445 1184
Size: 1261 5433
BstXI CCAn_nnnn'nTGG
Cuts at: 3021 3427 3736 3021
Size: 406 309 5979
Fragments arranged by size:
5979 406 309
BstYI r'GATC_y
Cuts at: 1972 2415 2619 3072 4024 4391 4776 4793 6245
Size: 443 204 453 952 367 385 17 1452
Cuts at: 6245 6331 6343 6689 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 952 453 443 385 367 346
204 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2943 3192 513
Size: 552 939 939 249 4015
Fragments arranged by size:
4015 939 939 552 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2443 3100 487
Size: 683 56 1217 657 4081
Fragments arranged by size:
4081 1217 683 657 56
BtsI GCAGTG_nn'
Cuts at: 3261 3612 4210 5004 5024 3261
Size: 351 598 794 20 4931
Fragments arranged by size:
4931 794 598 351 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6659
DraI TTT'AAA
Cuts at: 3998 3998
Size: 6694
DraIII CAC_nnn'GTG
Cuts at: 3103 3597 3103
Size: 494 6200
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5701 809
Size: 507 4385 1802
Fragments arranged by size:
4385 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2123 3726
Size: 54 1425 50 275 18 198 95 1603
Cuts at: 3726 3777 4141 5030 6659 8
Size: 51 364 889 1629 43
Fragments arranged by size:
1629 1603 1425 889 364 275 198 95
54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2123 4141 1537
Size: 275 311 2018 4090
Fragments arranged by size:
4090 2018 311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2507 2630 2772 3452 4624 1353
Size: 698 456 123 142 680 1172 3423
Fragments arranged by size:
3423 1172 698 680 456 142 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 5281 5655 5801 387
Size: 1064 138 528 3164 374 146 1280
Fragments arranged by size:
3164 1280 1064 528 374 146 138
EcoICRI GAG'CTC
Cuts at: 2589 2589
Size: 6694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2283 2558 2573 2734 2768 2792
Size: 393 497 154 275 15 161 34 24
Cuts at: 2792 2816 2933 3980 4757 5354 6141 6409 1239
Size: 24 117 1047 777 597 787 268 1524
Fragments arranged by size:
1524 1047 787 777 597 497 393 275
268 161 154 117 34 24 24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2330
Size: 6694
EcoO109I rG'GnC_Cy
Cuts at: 2037 2257 2258 3355 3478 3664 3854 2037
Size: 220 1 1097 123 186 190 4877
Fragments arranged by size:
4877 1097 220 190 186 123 1
EcoRI G'AATT_C
Cuts at: 2853 4003 4381 2853
Size: 1150 378 5166
Fragments arranged by size:
5166 1150 378
FspI TGC'GCA
Cuts at: 2201 5200 2201
Size: 2999 3695
HaeII r_GCGC'y
Cuts at: 1678 5841 1678
Size: 4163 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2400 2432 2947 2979 3064
Size: 32 135 32 828 32 515 32 85
Cuts at: 3064 3096 5335 5367 5409 5441 1373
Size: 32 2239 32 42 32 2626
Fragments arranged by size:
2626 2239 828 515 135 85 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3575 4038 4284 669
Size: 988 281 1637 463 246 3079
Fragments arranged by size:
3079 1637 988 463 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6694
HpaI GTT'AAC
Cuts at: 4284 4284
Size: 6694
KpnI G_GTAC'C
Cuts at: 4019 4019
Size: 6694
MfeI C'AATT_G
Cuts at: 4293 4293
Size: 6694
MluI A'CGCG_T
Cuts at: 2226 6570 2226
Size: 4344 2350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3005 3085 5808 5992 1305
Size: 1700 80 2723 184 2007
Fragments arranged by size:
2723 2007 1700 184 80
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2030 3728 3779 10
Size: 54 1425 343 198 1698 51 2925
Fragments arranged by size:
2925 1698 1425 343 198 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2249 3515 3734 4652 5011 5170
Size: 648 858 225 1266 219 918 359 159
Cuts at: 5170 518
Size: 2042
Fragments arranged by size:
2042 1266 918 858 648 359 225 219
159
MspA1I CmG'CkG
Cuts at: 1606 2119 2298 3310 3646 3969 4110 4787 5935
Size: 513 179 1012 336 323 141 677 1148
Cuts at: 5935 6180 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1012 677 513 336 323 245
179 141
NaeI GCC'GGC
Cuts at: 2127 2127
Size: 6694
NcoI C'CATG_G
Cuts at: 513 1065 2004 2943 3192 513
Size: 552 939 939 249 4015
Fragments arranged by size:
4015 939 939 552 249
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6694
NgoMIV G'CCGG_C
Cuts at: 2125 2125
Size: 6694
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6694
NotI GC'GGCC_GC
Cuts at: 4141 4141
Size: 6694
NspI r_CATG'y
Cuts at: 1132 1229 2374 3626 4052 5597 1132
Size: 97 1145 1252 426 1545 2229
Fragments arranged by size:
2229 1545 1252 1145 426 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6694
PciI A'CATG_T
Cuts at: 2370 5593 2370
Size: 3223 3471
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2215 3786 1108
Size: 1107 1571 4016
Fragments arranged by size:
4016 1571 1107
PfoI T'CCnGG_A
Cuts at: 2591 2591
Size: 6694
PmeI GTTT'AAAC
Cuts at: 3998 3998
Size: 6694
PmlI CAC'GTG
Cuts at: 3609 3609
Size: 6694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4763 4795 6276 6308 4763
Size: 32 1481 32 5149
Fragments arranged by size:
5149 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3478 3664 3478
Size: 186 6508
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6694
PsiI TTA'TAA
Cuts at: 4264 4264
Size: 6694
PspOMI G'GGCC_C
Cuts at: 2257 3210 2257
Size: 953 5741
PstI C_TGCA'G
Cuts at: 830 2118 2187 2467 2940 3492 4046 830
Size: 1288 69 280 473 552 554 3478
Fragments arranged by size:
3478 1288 554 552 473 280 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6694
PvuII CAG'CTG
Cuts at: 2119 3310 3646 3969 2119
Size: 1191 336 323 4844
Fragments arranged by size:
4844 1191 336 323
SacI G_AGCT'C
Cuts at: 2591 2591
Size: 6694
SalI G'TCGA_C
Cuts at: 1936 4036 1936
Size: 2100 4594
SanDI GG'GwC_CC
Cuts at: 3478 3664 3478
Size: 186 6508
SapI GCTCTTCn'nnn_
Cuts at: 2051 2772 2051
Size: 721 5973
SbfI CC_TGCA'GG
Cuts at: 4046 4046
Size: 6694
ScaI AGT'ACT
Cuts at: 4942 6654 4942
Size: 1712 4982
SexAI A'CCwGG_T
Cuts at: 3099 3099
Size: 6694
SfcI C'TryA_G
Cuts at: 826 1045 2114 2183 2463 2936 3488 4042 5177
Size: 219 1069 69 280 473 552 554 1135
Cuts at: 5177 5858 6049 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1069 681 554 552 473 280
219 191 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 2036 2036
Size: 6694
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6694
SmaI CCC'GGG
Cuts at: 1872 3209 4021 1872
Size: 1337 812 4545
Fragments arranged by size:
4545 1337 812
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4798 5699 5961 820
Size: 197 926 2855 901 262 1553
Fragments arranged by size:
2855 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4485 493
Size: 3992 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6694
SphI G_CATG'C
Cuts at: 1132 3626 4052 1132
Size: 2494 426 3774
Fragments arranged by size:
3774 2494 426
SspI AAT'ATT
Cuts at: 5 52 3845 4618 5
Size: 47 3793 773 2081
Fragments arranged by size:
3793 2081 773 47
StuI AGG'CCT
Cuts at: 1517 3494 3764 1517
Size: 1977 270 4447
Fragments arranged by size:
4447 1977 270
StyI C'CwwG_G
Cuts at: 513 1065 2004 2943 3192 3708 4133 513
Size: 552 939 939 249 516 425 3074
Fragments arranged by size:
3074 939 939 552 516 425 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2290 3555 3715 4721 4738 4891 5076
Size: 1255 710 1265 160 1006 17 153 185
Cuts at: 5076 5521 325
Size: 445 1498
Fragments arranged by size:
1498 1265 1255 1006 710 445 185 160
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3280 4940 6652
Size: 274 80 33 51 1443 1303 1660 1712
Cuts at: 6652 96
Size: 138
Fragments arranged by size:
1712 1660 1443 1303 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2213 2245 6654 6686 2213
Size: 32 4409 32 2221
Fragments arranged by size:
4409 2221 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6694
XbaI T'CTAG_A
Cuts at: 4030 4030
Size: 6694
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2702 2702
Size: 6694
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6694
XmaI C'CCGG_G
Cuts at: 1870 3207 4019 1870
Size: 1337 812 4545
Fragments arranged by size:
4545 1337 812
XmnI GAAnn'nnTTC
Cuts at: 2781 4823 2781
Size: 2042 4652
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4502 276
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacII SfoI SrfI SwaI