Restriction map for
pFN21AE2044
(Circular) (Six-base) MAPSORT of: pFN21AE2044.seq Check: 8103 from: 1 to: 6691
pFN21AE2044 6691 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3158 3550 3158
Size: 392 6299
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4501 278
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3296 4034 1937
Size: 1359 738 4594
Fragments arranged by size:
4594 1359 738
Acc65I G'GTAC_C
Cuts at: 4012 4012
Size: 6691
AclI AA'CG_TT
Cuts at: 1563 4818 5191 1563
Size: 3255 373 3063
Fragments arranged by size:
3255 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3598 4754 6138 6406 1632
Size: 1966 1156 1384 268 1917
Fragments arranged by size:
1966 1917 1384 1156 268
AfeI AGC'GCT
Cuts at: 3611 3611
Size: 6691
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6494
AflIII A'CryG_T
Cuts at: 1203 5590 6567 1203
Size: 4387 977 1327
Fragments arranged by size:
4387 1327 977
AgeI A'CCGG_T
Cuts at: 5522 5522
Size: 6691
AhdI GACnn_n'nnGTC
Cuts at: 3388 5420 3388
Size: 2032 4659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2085 3024 3641 1184
Size: 901 939 617 4234
Fragments arranged by size:
4234 939 901 617
AlwNI CAG_nnn'CTG
Cuts at: 3341 4434 6006 3341
Size: 1093 1572 4026
Fragments arranged by size:
4026 1572 1093
ApaI G_GGCC'C
Cuts at: 2144 2144
Size: 6691
ApaLI G'TGCA_C
Cuts at: 4748 5904 4748
Size: 1156 5535
ApoI r'AATT_y
Cuts at: 1493 2624 4000 4226 4378 6487 1493
Size: 1131 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 1131 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5245 160
Size: 872 4213 1606
Fragments arranged by size:
4213 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
AvaI C'yCGr_G
Cuts at: 1870 1943 2137 2932 3084 3275 3899 4016 1870
Size: 73 194 795 152 191 624 117 4545
Fragments arranged by size:
4545 795 624 194 191 152 117 73
AvrII C'CTAG_G
Cuts at: 2144 2144
Size: 6691
BamHI G'GATC_C
Cuts at: 3178 4021 4388 3178
Size: 843 367 5481
Fragments arranged by size:
5481 843 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2523 2827 3403 3729 3923
Size: 325 217 627 736 304 576 326 194
Cuts at: 3923 4012 5467 618
Size: 89 1455 1842
Fragments arranged by size:
1842 1455 736 627 576 326 325 304
217 194 89
BanII G_rGCy'C
Cuts at: 1391 2144 3091 4010 1391
Size: 753 947 919 4072
Fragments arranged by size:
4072 947 919 753
BbeI G_GCGC'C
Cuts at: 2831 2831
Size: 6691
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3474 3911 928
Size: 971 1575 437 3708
Fragments arranged by size:
3708 1575 971 437
BbvCI CC'TCA_GC
Cuts at: 2508 3334 3584 2508
Size: 826 250 5615
Fragments arranged by size:
5615 826 250
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 4882 4916 1903
Size: 34 1730 34 1181 34 3678
Fragments arranged by size:
3678 1730 1181 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3937 4583 5793 3937
Size: 646 1210 4835
Fragments arranged by size:
4835 1210 646
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6691
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5302 136
Size: 107 122 71 631 605 3630 1525
Fragments arranged by size:
3630 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6686 6686
Size: 6691
BlpI GC'TnA_GC
Cuts at: 2281 3416 4108 2281
Size: 1135 692 4864
Fragments arranged by size:
4864 1135 692
Bme1580I G_kGCm'C
Cuts at: 1790 2144 3406 3740 3928 4752 5908 1790
Size: 354 1262 334 188 824 1156 2573
Fragments arranged by size:
2573 1262 1156 824 354 334 188
BmgBI CAC'GTC
Cuts at: 1318 2997 3358 1318
Size: 1679 361 4651
Fragments arranged by size:
4651 1679 361
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3409 3809 4422
Size: 416 53 83 399 24 1992 400 613
Cuts at: 4422 5380 442
Size: 958 1753
Fragments arranged by size:
1992 1753 958 613 416 400 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 2689 2819 3226 3616 3804 5351 1239
Size: 1385 65 130 407 390 188 1547 2579
Fragments arranged by size:
2579 1547 1385 407 390 188 130 65
Bpu10I CC'TnA_GC
Cuts at: 2246 2508 3334 3584 3674 3930 2246
Size: 262 826 250 90 256 5007
Fragments arranged by size:
5007 826 262 256 250 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4816 5681 5979 4816
Size: 865 298 5528
Fragments arranged by size:
5528 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2477 3503 5354 882
Size: 617 978 1026 1851 2219
Fragments arranged by size:
2219 1851 1026 978 617
BsaAI yAC'GTr
Cuts at: 493 1204 4482 493
Size: 711 3278 2702
Fragments arranged by size:
3278 2702 711
BsaBI GATnn'nnATC
Cuts at: 6685 6685
Size: 6691
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2828 3432 3693 4498
Size: 53 83 186 936 1295 604 261 805
Cuts at: 4498 4880 5515 275
Size: 382 635 1451
Fragments arranged by size:
1451 1295 936 805 635 604 382 261
186 83 53
BsaWI w'CCGG_w
Cuts at: 2113 2526 5124 5522 5796 5943 6354 2113
Size: 413 2598 398 274 147 411 2450
Fragments arranged by size:
2598 2450 413 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2174 2204 2844 2874 1178
Size: 30 165 30 771 30 640 30 4995
Fragments arranged by size:
4995 771 640 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2418 2776 2953 1186
Size: 841 391 358 177 4924
Fragments arranged by size:
4924 841 391 358 177
BseYI C'CCAG_C
Cuts at: 2216 2312 2534 2647 2746 2793 3341 3606 5894
Size: 96 222 113 99 47 548 265 2288
Cuts at: 5894 6390 2216
Size: 496 2517
Fragments arranged by size:
2517 2288 548 496 265 222 113 99
96 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2534 2891 3518 3578 3647 1163
Size: 573 798 357 627 60 69 4207
Fragments arranged by size:
4207 798 627 573 357 69 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4141 4902 5930 1540
Size: 275 186 2140 761 1028 2301
Fragments arranged by size:
2301 2140 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2192 3422 4752 4837 5908 2192
Size: 1230 1330 85 1071 2975
Fragments arranged by size:
2975 1330 1230 1071 85
BsmI GAATG_Cn'
Cuts at: 4202 4295 4202
Size: 93 6598
BsmBI CGTCTCn'nnnn_
Cuts at: 3364 3364
Size: 6691
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2192 3091 3406 3422 3740 3928
Size: 399 354 48 899 315 16 318 188
Cuts at: 3928 4010 4752 4837 5908 1391
Size: 82 742 85 1071 2174
Fragments arranged by size:
2174 1071 899 742 399 354 318 315
188 85 82 48 16
BspEI T'CCGG_A
Cuts at: 2113 6354 2113
Size: 4241 2450
BspHI T'CATG_A
Cuts at: 4580 6310 4580
Size: 1730 4961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3158 3550 3619 4046 844
Size: 2314 392 69 427 3489
Fragments arranged by size:
3489 2314 427 392 69
BsrBI CCG'CTC
Cuts at: 1394 1823 4578 1394
Size: 429 2755 3507
Fragments arranged by size:
3507 2755 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3231 3334 5186 5360 65
Size: 1183 1983 103 1852 174 1396
Fragments arranged by size:
1983 1852 1396 1183 174 103
BsrFI r'CCGG_y
Cuts at: 1700 1810 2276 5335 5522 1700
Size: 110 466 3059 187 2869
Fragments arranged by size:
3059 2869 466 187 110
BsrGI T'GTAC_A
Cuts at: 96 2328 96
Size: 2232 4459
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6691
BssSI C'ACGA_G
Cuts at: 3686 4751 5763 3686
Size: 1065 1012 4614
Fragments arranged by size:
4614 1065 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3496 794
Size: 2702 3989
BstBI TT'CG_AA
Cuts at: 5495 5495
Size: 6691
BstEII G'GTnAC_C
Cuts at: 1184 2017 2389 1184
Size: 833 372 5486
Fragments arranged by size:
5486 833 372
BstYI r'GATC_y
Cuts at: 1972 3178 4021 4388 4773 4790 6242 6328 6340
Size: 1206 843 367 385 17 1452 86 12
Cuts at: 6340 6686 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1206 843 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2385 2568 2721 3918 2385
Size: 183 153 1197 5158
Fragments arranged by size:
5158 1197 183 153
BtgI C'CryG_G
Cuts at: 513 1065 2004 2543 513
Size: 552 939 539 4661
Fragments arranged by size:
4661 939 552 539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5952
Fragments arranged by size:
5952 683 56
BtsI GCAGTG_nn'
Cuts at: 3809 4207 5001 5021 3809
Size: 398 794 20 5479
Fragments arranged by size:
5479 794 398 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6656
DraI TTT'AAA
Cuts at: 3995 3995
Size: 6691
DraIII CAC_nnn'GTG
Cuts at: 3291 3579 3758 3291
Size: 288 179 6224
Fragments arranged by size:
6224 288 179
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2377 5698 809
Size: 507 1061 3321 1802
Fragments arranged by size:
3321 1802 1061 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2457 3077 4138
Size: 54 1425 50 275 18 627 620 1061
Cuts at: 4138 5027 6656 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1061 889 627 620 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4138 1537
Size: 275 2326 4090
Fragments arranged by size:
4090 2326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 2051 3825 4621 1353
Size: 692 6 1774 796 3423
Fragments arranged by size:
3423 1774 796 692 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3757 5278 5652 5798 387
Size: 1064 138 2168 1521 374 146 1280
Fragments arranged by size:
2168 1521 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2624 2689 2819 3226 3598 3616 3804
Size: 393 992 65 130 407 372 18 188
Cuts at: 3804 4754 5351 6138 6406 1239
Size: 950 597 787 268 1524
Fragments arranged by size:
1524 992 950 787 597 407 393 372
268 188 130 65 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2359 3845 2359
Size: 1486 5205
EcoO109I rG'GnC_Cy
Cuts at: 2101 2141 2149 2207 2901 3139 3850 2101
Size: 40 8 58 694 238 711 4942
Fragments arranged by size:
4942 711 694 238 58 40 8
EcoRI G'AATT_C
Cuts at: 2624 4000 4378 2624
Size: 1376 378 4937
Fragments arranged by size:
4937 1376 378
EcoRV GAT'ATC
Cuts at: 2169 2169
Size: 6691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3304 3304
Size: 6691
FspI TGC'GCA
Cuts at: 3270 5197 3270
Size: 1927 4764
HaeII r_GCGC'y
Cuts at: 1678 2070 2260 2831 3613 5838 1678
Size: 392 190 571 782 2225 2531
Fragments arranged by size:
2531 2225 782 571 392 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2114 2140 2146 2178
Size: 32 135 32 536 6 26 6 32
Cuts at: 2178 2570 2602 2844 2876 2911 2943 3374 3406
Size: 392 32 242 32 35 32 431 32
Cuts at: 3406 3409 3441 3880 3912 5332 5364 5406 5438
Size: 3 32 439 32 1420 32 42 32
Cuts at: 5438 1373
Size: 2626
Fragments arranged by size:
2626 1420 536 439 431 392 242 135
42 35 32 32 32 32 32 32
32 32 32 32 32 26 6 6
3
HincII GTy'rAC
Cuts at: 669 1657 1938 4035 4281 669
Size: 988 281 2097 246 3079
Fragments arranged by size:
3079 2097 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6691
HpaI GTT'AAC
Cuts at: 4281 4281
Size: 6691
KasI G'GCGC_C
Cuts at: 2827 2827
Size: 6691
KpnI G_GTAC'C
Cuts at: 4016 4016
Size: 6691
MfeI C'AATT_G
Cuts at: 4290 4290
Size: 6691
MluI A'CGCG_T
Cuts at: 6567 6567
Size: 6691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2328 2659 3031 5805 5989 1305
Size: 1023 331 372 2774 184 2007
Fragments arranged by size:
2774 2007 1023 372 331 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3079 10
Size: 54 1425 343 1247 3622
Fragments arranged by size:
3622 1425 1247 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 3623 4649 5008 5167 518
Size: 648 1278 1179 1026 359 159 2042
Fragments arranged by size:
2042 1278 1179 1026 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2216 2467 2647 2746 2797 3204 3341
Size: 408 202 251 180 99 51 407 137
Cuts at: 3341 3456 3745 4107 4784 5932 6177 1606
Size: 115 289 362 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 408 407 362 289 251
245 202 180 137 115 99 51
NaeI GCC'GGC
Cuts at: 2278 2278
Size: 6691
NarI GG'CG_CC
Cuts at: 2828 2828
Size: 6691
NcoI C'CATG_G
Cuts at: 513 1065 2004 2543 513
Size: 552 939 539 4661
Fragments arranged by size:
4661 939 552 539
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6691
NgoMIV G'CCGG_C
Cuts at: 2276 2276
Size: 6691
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6691
NotI GC'GGCC_GC
Cuts at: 4138 4138
Size: 6691
NspI r_CATG'y
Cuts at: 1132 1229 2869 4049 5594 1132
Size: 97 1640 1180 1545 2229
Fragments arranged by size:
2229 1640 1545 1180 97
PasI CC'CwG_GG
Cuts at: 1348 3661 1348
Size: 2313 4378
PciI A'CATG_T
Cuts at: 5590 5590
Size: 6691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3075 1108
Size: 1967 4724
PfoI T'CCnGG_A
Cuts at: 2666 3825 2666
Size: 1159 5532
PmeI GTTT'AAAC
Cuts at: 3995 3995
Size: 6691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2501 2533 4760 4792 6273 6305 2501
Size: 32 2227 32 1481 32 2887
Fragments arranged by size:
2887 2227 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2101 2149 2207 2901 2101
Size: 48 58 694 5891
Fragments arranged by size:
5891 694 58 48
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6691
PsiI TTA'TAA
Cuts at: 4261 4261
Size: 6691
PspOMI G'GGCC_C
Cuts at: 2140 2140
Size: 6691
PstI C_TGCA'G
Cuts at: 830 2078 2298 2400 3155 3846 4043 830
Size: 1248 220 102 755 691 197 3478
Fragments arranged by size:
3478 1248 755 691 220 197 102
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6691
PvuII CAG'CTG
Cuts at: 2216 2467 2647 2746 2797 3204 3341 2216
Size: 251 180 99 51 407 137 5566
Fragments arranged by size:
5566 407 251 180 137 99 51
SalI G'TCGA_C
Cuts at: 1936 4033 1936
Size: 2097 4594
SanDI GG'GwC_CC
Cuts at: 2207 2207
Size: 6691
SbfI CC_TGCA'GG
Cuts at: 3846 4043 3846
Size: 197 6494
ScaI AGT'ACT
Cuts at: 4939 6651 4939
Size: 1712 4979
SexAI A'CCwGG_T
Cuts at: 2496 2496
Size: 6691
SfcI C'TryA_G
Cuts at: 826 1045 2074 2294 2396 2855 3151 3199 3842
Size: 219 1029 220 102 459 296 48 643
Cuts at: 3842 4039 5174 5855 6046 826
Size: 197 1135 681 191 1471
Fragments arranged by size:
1471 1135 1029 681 643 459 296 220
219 197 191 102 48
SfoI GGC'GCC
Cuts at: 2829 2829
Size: 6691
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6691
SmaI CCC'GGG
Cuts at: 1872 3086 4018 1872
Size: 1214 932 4545
Fragments arranged by size:
4545 1214 932
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3899 4795 5696 5958 820
Size: 197 926 1956 896 901 262 1553
Fragments arranged by size:
1956 1553 926 901 896 262 197
SnaBI TAC'GTA
Cuts at: 493 4482 493
Size: 3989 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6691
SphI G_CATG'C
Cuts at: 1132 4049 1132
Size: 2917 3774
SrfI GCCC'GGGC
Cuts at: 3086 3086
Size: 6691
SspI AAT'ATT
Cuts at: 5 52 4615 5
Size: 47 4563 2081
Fragments arranged by size:
4563 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6691
StyI C'CwwG_G
Cuts at: 513 1065 2004 2144 2543 2656 2889 3697 3706
Size: 552 939 140 399 113 233 808 9
Cuts at: 3706 3957 4130 513
Size: 251 173 3074
Fragments arranged by size:
3074 939 808 552 399 251 233 173
140 113 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2014 2335 2548 3032 4718 4735 4888
Size: 1255 434 321 213 484 1686 17 153
Cuts at: 4888 5073 5518 325
Size: 185 445 1498
Fragments arranged by size:
1686 1498 1255 484 445 434 321 213
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2328 4937 6649
Size: 274 80 33 51 1443 351 2609 1712
Cuts at: 6649 96
Size: 138
Fragments arranged by size:
2609 1712 1443 351 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2158 2190 2855 2887 6651 6683 2158
Size: 32 665 32 3764 32 2166
Fragments arranged by size:
3764 2166 665 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6691
XbaI T'CTAG_A
Cuts at: 4027 4027
Size: 6691
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2978 2011
Size: 967 5724
XhoI C'TCGA_G
Cuts at: 1943 3899 1943
Size: 1956 4735
XmaI C'CCGG_G
Cuts at: 1870 3084 4016 1870
Size: 1214 932 4545
Fragments arranged by size:
4545 1214 932
XmnI GAAnn'nnTTC
Cuts at: 4820 4820
Size: 6691
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4499 276
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BplI BsiWI BstXI BstZ17I
EcoICRI FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SapI SfiI SwaI