Restriction map for
pFN21AE2043
(Circular) (Six-base) MAPSORT of: pFN21AE2043.seq Check: 2008 from: 1 to: 6691
pFN21AE2043 6691 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3189 3617 3189
Size: 428 6263
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4501 278
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4034 1937
Size: 2097 4594
Acc65I G'GTAC_C
Cuts at: 2691 4012 2691
Size: 1321 5370
AclI AA'CG_TT
Cuts at: 1563 4818 5191 1563
Size: 3255 373 3063
Fragments arranged by size:
3255 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2352 3893 4754 6138 6406 1632
Size: 720 1541 861 1384 268 1917
Fragments arranged by size:
1917 1541 1384 861 720 268
AflII C'TTAA_G
Cuts at: 820 1017 2216 820
Size: 197 1199 5295
Fragments arranged by size:
5295 1199 197
AflIII A'CryG_T
Cuts at: 1203 5590 6567 1203
Size: 4387 977 1327
Fragments arranged by size:
4387 1327 977
AgeI A'CCGG_T
Cuts at: 2421 5522 2421
Size: 3101 3590
AhdI GACnn_n'nnGTC
Cuts at: 3498 5420 3498
Size: 1922 4769
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2910 1184
Size: 1726 4965
AlwNI CAG_nnn'CTG
Cuts at: 2371 2890 4434 6006 2371
Size: 519 1544 1572 3056
Fragments arranged by size:
3056 1572 1544 519
ApaI G_GGCC'C
Cuts at: 2261 3535 3854 2261
Size: 1274 319 5098
Fragments arranged by size:
5098 1274 319
ApaLI G'TGCA_C
Cuts at: 3632 4748 5904 3632
Size: 1116 1156 4419
Fragments arranged by size:
4419 1156 1116
ApoI r'AATT_y
Cuts at: 1493 3240 4000 4226 4378 6487 1493
Size: 1747 760 226 152 2109 1697
Fragments arranged by size:
2109 1747 1697 760 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5245 160
Size: 872 4213 1606
Fragments arranged by size:
4213 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 3567 4016 1870
Size: 73 165 1459 449 4545
Fragments arranged by size:
4545 1459 449 165 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2153 2186 2153
Size: 33 6658
BamHI G'GATC_C
Cuts at: 2975 3007 4021 4388 2975
Size: 32 1014 367 5278
Fragments arranged by size:
5278 1014 367 32
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2208 2336 2691 2708 3135
Size: 325 217 627 421 128 355 17 427
Cuts at: 3135 3664 4012 5467 618
Size: 529 348 1455 1842
Fragments arranged by size:
1842 1455 627 529 427 421 355 348
325 217 128 17
BanII G_rGCy'C
Cuts at: 1391 2261 3360 3535 3854 4010 1391
Size: 870 1099 175 319 156 4072
Fragments arranged by size:
4072 1099 870 319 175 156
BbeI G_GCGC'C
Cuts at: 2712 2712
Size: 6691
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2514 3366 928
Size: 971 615 852 4253
Fragments arranged by size:
4253 971 852 615
BbvCI CC'TCA_GC
Cuts at: 2726 3108 2726
Size: 382 6309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2806 2840 3673 3707 4882 4916 1903
Size: 34 869 34 833 34 1175 34 3678
Fragments arranged by size:
3678 1175 869 833 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4583 5793 4583
Size: 1210 5481
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6691
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5302 136
Size: 107 122 71 631 605 3630 1525
Fragments arranged by size:
3630 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6686 6686
Size: 6691
BlpI GC'TnA_GC
Cuts at: 2649 4108 2649
Size: 1459 5232
Bme1580I G_kGCm'C
Cuts at: 1790 2261 3535 3636 3854 4752 5908 1790
Size: 471 1274 101 218 898 1156 2573
Fragments arranged by size:
2573 1274 1156 898 471 218 101
BmgBI CAC'GTC
Cuts at: 1318 3463 1318
Size: 2145 4546
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2043 3228 4422
Size: 416 53 83 399 24 626 1185 1194
Cuts at: 4422 5380 442
Size: 958 1753
Fragments arranged by size:
1753 1194 1185 958 626 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2751 2816 2828 2942 3321 3743 3752 5351
Size: 1512 65 12 114 379 422 9 1599
Cuts at: 5351 1239
Size: 2579
Fragments arranged by size:
2579 1599 1512 422 379 114 65 12
9
Bpu10I CC'TnA_GC
Cuts at: 2321 2726 3108 3855 2321
Size: 405 382 747 5157
Fragments arranged by size:
5157 747 405 382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 3275 4816 5681 5979 2135
Size: 1140 1541 865 298 2847
Fragments arranged by size:
2847 1541 1140 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 3232 5354 882
Size: 617 516 1217 2122 2219
Fragments arranged by size:
2219 2122 1217 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 3280 4482 493
Size: 711 2076 1202 2702
Fragments arranged by size:
2702 2076 1202 711
BsaBI GATnn'nnATC
Cuts at: 3776 6685 3776
Size: 2909 3782
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2226 2316 2709 3799
Size: 53 83 186 936 693 90 393 1090
Cuts at: 3799 4498 4880 5515 275
Size: 699 382 635 1451
Fragments arranged by size:
1451 1090 936 699 693 635 393 382
186 90 83 53
BsaWI w'CCGG_w
Cuts at: 2164 2421 2616 3921 5124 5522 5796 5943 6354
Size: 257 195 1305 1203 398 274 147 411
Cuts at: 6354 2164
Size: 2501
Fragments arranged by size:
2501 1305 1203 411 398 274 257 195
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2196 2226 3264 3294 1178
Size: 30 165 30 793 30 1038 30 4575
Fragments arranged by size:
4575 1038 793 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3848 3851 3902 1186
Size: 2662 3 51 3975
Fragments arranged by size:
3975 2662 51 3
BseYI C'CCAG_C
Cuts at: 2298 2462 2790 3039 3790 3962 5894 6390 2298
Size: 164 328 249 751 172 1932 496 2599
Fragments arranged by size:
2599 1932 751 496 328 249 172 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2090 2273 3302 3595 3664 1163
Size: 573 354 183 1029 293 69 4190
Fragments arranged by size:
4190 1029 573 354 293 183 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2344 3352 4141 4902 5930 1540
Size: 275 186 343 1008 789 761 1028 2301
Fragments arranged by size:
2301 1028 1008 789 761 343 275 186
BsiHKAI G_wGCw'C
Cuts at: 3263 3636 4752 4837 5908 3263
Size: 373 1116 85 1071 4046
Fragments arranged by size:
4046 1116 1071 373 85
BsmI GAATG_Cn'
Cuts at: 3931 4202 4295 3931
Size: 271 93 6327
Fragments arranged by size:
6327 271 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2261 3263 3360 3535 3636 3854 4010
Size: 399 471 1002 97 175 101 218 156
Cuts at: 4010 4752 4837 5908 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1002 742 471 399 218 175
156 101 97 85
BspEI T'CCGG_A
Cuts at: 3921 6354 3921
Size: 2433 4258
BspHI T'CATG_A
Cuts at: 4580 6310 4580
Size: 1730 4961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3189 3617 3963 4046 844
Size: 2345 428 346 83 3489
Fragments arranged by size:
3489 2345 428 346 83
BsrBI CCG'CTC
Cuts at: 1394 1823 4578 1394
Size: 429 2755 3507
Fragments arranged by size:
3507 2755 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5186 5360 65
Size: 1183 3938 174 1396
Fragments arranged by size:
3938 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 2339 2421 3614 5335 5522 1700
Size: 110 241 288 82 1193 1721 187 2869
Fragments arranged by size:
2869 1721 1193 288 241 187 110 82
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6691
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6691
BssSI C'ACGA_G
Cuts at: 4751 5763 4751
Size: 1012 5679
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6691
BstBI TT'CG_AA
Cuts at: 2826 5495 2826
Size: 2669 4022
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6691
BstXI CCAn_nnnn'nTGG
Cuts at: 2548 2719 3021 3970 2548
Size: 171 302 949 5269
Fragments arranged by size:
5269 949 302 171
BstYI r'GATC_y
Cuts at: 1972 2244 2599 2975 3007 3918 4021 4388 4773
Size: 272 355 376 32 911 103 367 385
Cuts at: 4773 4790 6242 6328 6340 6686 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 911 385 376 367 355 346
272 103 86 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 2364 3861 2364
Size: 1497 5194
BtgI C'CryG_G
Cuts at: 513 1065 2004 2718 2943 513
Size: 552 939 714 225 4261
Fragments arranged by size:
4261 939 714 552 225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5952
Fragments arranged by size:
5952 683 56
BtsI GCAGTG_nn'
Cuts at: 3740 4207 5001 5021 3740
Size: 467 794 20 5410
Fragments arranged by size:
5410 794 467 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6656
DraI TTT'AAA
Cuts at: 3995 3995
Size: 6691
DraIII CAC_nnn'GTG
Cuts at: 3597 3597
Size: 6691
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5698 809
Size: 507 4382 1802
Fragments arranged by size:
4382 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2341 2538
Size: 54 1425 50 275 18 256 255 197
Cuts at: 2538 2550 3030 3123 3345 3616 3984 4138 5027
Size: 12 480 93 222 271 368 154 889
Cuts at: 5027 6656 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 480 368 275 271 256
255 222 197 154 93 54 50 43
18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2341 4138 1537
Size: 275 529 1797 4090
Fragments arranged by size:
4090 1797 529 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2388 2397 4621 1353
Size: 1035 9 2224 3423
Fragments arranged by size:
3423 2224 1035 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5278 5652 5798 387
Size: 1064 138 3689 374 146 1280
Fragments arranged by size:
3689 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2352 2751 2816 2828 2942 3321 3743
Size: 393 720 399 65 12 114 379 422
Cuts at: 3743 3752 3893 4754 5351 6138 6406 1239
Size: 9 141 861 597 787 268 1524
Fragments arranged by size:
1524 861 787 720 597 422 399 393
379 268 141 114 65 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2216 3905 2216
Size: 1689 5002
EcoO109I rG'GnC_Cy
Cuts at: 2037 2257 2258 2359 2368 3531 3851 2037
Size: 220 1 101 9 1163 320 4877
Fragments arranged by size:
4877 1163 320 220 101 9 1
EcoRI G'AATT_C
Cuts at: 4000 4378 4000
Size: 378 6313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3394 3394
Size: 6691
FspI TGC'GCA
Cuts at: 2201 3047 5197 2201
Size: 846 2150 3695
Fragments arranged by size:
3695 2150 846
HaeII r_GCGC'y
Cuts at: 1678 2108 2712 2734 5838 1678
Size: 430 604 22 3104 2531
Fragments arranged by size:
3104 2531 604 430 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3062 3094 3484 3516 3641
Size: 32 135 32 1490 32 390 32 125
Cuts at: 3641 3673 5332 5364 5406 5438 1373
Size: 32 1659 32 42 32 2626
Fragments arranged by size:
2626 1659 1490 390 135 125 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2521 4035 4281 669
Size: 988 281 583 1514 246 3079
Fragments arranged by size:
3079 1514 988 583 281 246
HindIII A'AGCT_T
Cuts at: 748 3164 748
Size: 2416 4275
HpaI GTT'AAC
Cuts at: 4281 4281
Size: 6691
KasI G'GCGC_C
Cuts at: 2708 2708
Size: 6691
KpnI G_GTAC'C
Cuts at: 2695 4016 2695
Size: 1321 5370
MfeI C'AATT_G
Cuts at: 4290 4290
Size: 6691
MluI A'CGCG_T
Cuts at: 6567 6567
Size: 6691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2082 5805 5989 1305
Size: 777 3723 184 2007
Fragments arranged by size:
3723 2007 777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2540 3032 3125 3986 10
Size: 54 1425 343 708 492 93 861 2715
Fragments arranged by size:
2715 1425 861 708 492 343 93 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 3119 4649 5008 5167 518
Size: 648 858 1095 1530 359 159 2042
Fragments arranged by size:
2042 1530 1095 858 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2182 2298 2794 2962 3286 3646 3790
Size: 468 108 116 496 168 324 360 144
Cuts at: 3790 4107 4784 5932 6177 1606
Size: 317 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 496 468 360 324 317
245 168 144 116 108
NarI GG'CG_CC
Cuts at: 2709 2709
Size: 6691
NcoI C'CATG_G
Cuts at: 513 1065 2004 2943 513
Size: 552 939 939 4261
Fragments arranged by size:
4261 939 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6691
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6691
NotI GC'GGCC_GC
Cuts at: 4138 4138
Size: 6691
NspI r_CATG'y
Cuts at: 1132 1229 3943 4049 5594 1132
Size: 97 2714 106 1545 2229
Fragments arranged by size:
2714 2229 1545 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6691
PciI A'CATG_T
Cuts at: 5590 5590
Size: 6691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3039 3783 1108
Size: 1931 744 4016
Fragments arranged by size:
4016 1931 744
PfoI T'CCnGG_A
Cuts at: 2666 2666
Size: 6691
PmeI GTTT'AAAC
Cuts at: 3995 3995
Size: 6691
PmlI CAC'GTG
Cuts at: 3280 3280
Size: 6691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2267 2299 4760 4792 6273 6305 2267
Size: 32 2461 32 1481 32 2653
Fragments arranged by size:
2653 2461 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2359
Size: 6691
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6691
PsiI TTA'TAA
Cuts at: 3170 4261 3170
Size: 1091 5600
PspOMI G'GGCC_C
Cuts at: 2257 3531 3850 2257
Size: 1274 319 5098
Fragments arranged by size:
5098 1274 319
PstI C_TGCA'G
Cuts at: 830 2787 3078 3492 4043 830
Size: 1957 291 414 551 3478
Fragments arranged by size:
3478 1957 551 414 291
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6691
PvuII CAG'CTG
Cuts at: 2074 2794 2962 3286 3646 3790 2074
Size: 720 168 324 360 144 4975
Fragments arranged by size:
4975 720 360 324 168 144
SalI G'TCGA_C
Cuts at: 1936 4033 1936
Size: 2097 4594
SapI GCTCTTCn'nnn_
Cuts at: 2388 2388
Size: 6691
SbfI CC_TGCA'GG
Cuts at: 4043 4043
Size: 6691
ScaI AGT'ACT
Cuts at: 4939 6651 4939
Size: 1712 4979
SexAI A'CCwGG_T
Cuts at: 2064 3494 3573 2064
Size: 1430 79 5182
Fragments arranged by size:
5182 1430 79
SfcI C'TryA_G
Cuts at: 826 1045 2783 2875 3074 3316 3488 4039 5174
Size: 219 1738 92 199 242 172 551 1135
Cuts at: 5174 5855 6046 826
Size: 681 191 1471
Fragments arranged by size:
1738 1471 1135 681 551 242 219 199
191 172 92
SfoI GGC'GCC
Cuts at: 2710 2710
Size: 6691
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6691
SmaI CCC'GGG
Cuts at: 1872 4018 1872
Size: 2146 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 2216 3290 4795 5696 5958
Size: 197 926 207 66 1074 1505 901 262
Cuts at: 5958 820
Size: 1553
Fragments arranged by size:
1553 1505 1074 926 901 262 207 197
66
SnaBI TAC'GTA
Cuts at: 493 4482 493
Size: 3989 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6691
SphI G_CATG'C
Cuts at: 1132 4049 1132
Size: 2917 3774
SspI AAT'ATT
Cuts at: 5 52 4615 5
Size: 47 4563 2081
Fragments arranged by size:
4563 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6691
StyI C'CwwG_G
Cuts at: 513 1065 2004 2541 2943 3708 4130 513
Size: 552 939 537 402 765 422 3074
Fragments arranged by size:
3074 939 765 552 537 422 402
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2290 3031 3555 3652 4718 4735 4888
Size: 1255 710 741 524 97 1066 17 153
Cuts at: 4888 5073 5518 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1066 741 710 524 445 185
153 97 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4937 6649 96
Size: 274 80 33 51 1443 2960 1712 138
Fragments arranged by size:
2960 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3264 3296 6651 6683 3264
Size: 32 3355 32 3272
Fragments arranged by size:
3355 3272 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2276 1316
Size: 960 5731
XbaI T'CTAG_A
Cuts at: 2602 4027 2602
Size: 1425 5266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2702 3220 2702
Size: 518 6173
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6691
XmaI C'CCGG_G
Cuts at: 1870 4016 1870
Size: 2146 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 4820 3878
Size: 942 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4499 276
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI RsrII SacI SacII SanDI
SfiI SrfI SwaI