Restriction map for
pFN21AE2041
(Circular) (Six-base) MAPSORT of: pFN21AE2041.seq Check: 3867 from: 1 to: 6664
pFN21AE2041 6664 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4474 278
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2670 4007 1937
Size: 733 1337 4594
Fragments arranged by size:
4594 1337 733
Acc65I G'GTAC_C
Cuts at: 2082 3985 2082
Size: 1903 4761
AclI AA'CG_TT
Cuts at: 1563 3615 4791 5164 1563
Size: 2052 1176 373 3063
Fragments arranged by size:
3063 2052 1176 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 2474 4727 6111 6379 1632
Size: 494 348 2253 1384 268 1917
Fragments arranged by size:
2253 1917 1384 494 348 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6467
AflIII A'CryG_T
Cuts at: 1203 3624 5563 6540 1203
Size: 2421 1939 977 1327
Fragments arranged by size:
2421 1939 1327 977
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6664
AlwNI CAG_nnn'CTG
Cuts at: 4407 5979 4407
Size: 1572 5092
ApaLI G'TGCA_C
Cuts at: 4721 5877 4721
Size: 1156 5508
ApoI r'AATT_y
Cuts at: 1493 2467 2626 2748 2801 2887 3773 3973 4199
Size: 974 159 122 53 86 886 200 226
Cuts at: 4199 4351 6460 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 974 886 226 200 159 152
122 86 53
AseI AT'TA_AT
Cuts at: 160 1032 5218 160
Size: 872 4186 1606
Fragments arranged by size:
4186 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 3989 1870
Size: 73 2046 4545
Fragments arranged by size:
4545 2046 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2074 2107 2817 2850 2074
Size: 33 710 33 5888
Fragments arranged by size:
5888 710 33 33
BamHI G'GATC_C
Cuts at: 3994 4361 3994
Size: 367 6297
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2082 3985 5440 618
Size: 325 217 627 295 1903 1455 1842
Fragments arranged by size:
1903 1842 1455 627 325 295 217
BanII G_rGCy'C
Cuts at: 1391 3983 1391
Size: 2592 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3936 928
Size: 971 2037 3656
Fragments arranged by size:
3656 2037 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4855 4889 1903
Size: 34 2918 34 3678
Fragments arranged by size:
3678 2918 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3397 4556 5766 3397
Size: 1159 1210 4295
Fragments arranged by size:
4295 1210 1159
BclI T'GATC_A
Cuts at: 1548 2067 3745 1548
Size: 519 1678 4467
Fragments arranged by size:
4467 1678 519
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5275 136
Size: 107 122 71 631 605 3603 1525
Fragments arranged by size:
3603 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2039 3707 6659 2039
Size: 1668 2952 2044
Fragments arranged by size:
2952 2044 1668
BlpI GC'TnA_GC
Cuts at: 4081 4081
Size: 6664
Bme1580I G_kGCm'C
Cuts at: 1790 4725 5881 1790
Size: 2935 1156 2573
Fragments arranged by size:
2935 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6664
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4395 5353 442
Size: 416 53 83 399 24 2978 958 1753
Fragments arranged by size:
2978 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5324 1239
Size: 4085 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2399 4789 5654 5952 2399
Size: 2390 865 298 3111
Fragments arranged by size:
3111 2390 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2573 2867 2949 3213 5327 882
Size: 617 1074 294 82 264 2114 2219
Fragments arranged by size:
2219 2114 1074 617 294 264 82
BsaAI yAC'GTr
Cuts at: 493 1204 3126 4455 493
Size: 711 1922 1329 2702
Fragments arranged by size:
2702 1922 1329 711
BsaBI GATnn'nnATC
Cuts at: 3200 3706 6658 3200
Size: 506 2952 3206
Fragments arranged by size:
3206 2952 506
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4471 4853 5488 275
Size: 53 83 186 936 2938 382 635 1451
Fragments arranged by size:
2938 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5097 5495 5769 5916 6327 5097
Size: 398 274 147 411 5434
Fragments arranged by size:
5434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6439
Fragments arranged by size:
6439 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3147 3321 3330 1186
Size: 1961 174 9 4520
Fragments arranged by size:
4520 1961 174 9
BseYI C'CCAG_C
Cuts at: 5867 6363 5867
Size: 496 6168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2669 1163
Size: 573 933 5158
Fragments arranged by size:
5158 933 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4114 4875 5903 1540
Size: 275 186 2113 761 1028 2301
Fragments arranged by size:
2301 2113 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4725 4810 5881 4725
Size: 85 1071 5508
Fragments arranged by size:
5508 1071 85
BsmI GAATG_Cn'
Cuts at: 4175 4268 4175
Size: 93 6571
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3983 4725 4810 5881 1391
Size: 399 2193 742 85 1071 2174
Fragments arranged by size:
2193 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6327 6327
Size: 6664
BspHI T'CATG_A
Cuts at: 3211 3244 4553 6283 3211
Size: 33 1309 1730 3592
Fragments arranged by size:
3592 1730 1309 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2691 4019 844
Size: 1847 1328 3489
Fragments arranged by size:
3489 1847 1328
BsrBI CCG'CTC
Cuts at: 1394 1823 4551 1394
Size: 429 2728 3507
Fragments arranged by size:
3507 2728 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5159 5333 65
Size: 1183 3911 174 1396
Fragments arranged by size:
3911 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5308 5495 1700
Size: 110 3498 187 2869
Fragments arranged by size:
3498 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3661 3696 96
Size: 3565 35 3064
Fragments arranged by size:
3565 3064 35
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6664
BssSI C'ACGA_G
Cuts at: 3471 4724 5736 3471
Size: 1253 1012 4399
Fragments arranged by size:
4399 1253 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6664
BstBI TT'CG_AA
Cuts at: 3584 5468 3584
Size: 1884 4780
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6664
BstXI CCAn_nnnn'nTGG
Cuts at: 3856 3856
Size: 6664
BstYI r'GATC_y
Cuts at: 1972 2039 2637 3029 3572 3707 3994 4361 4746
Size: 67 598 392 543 135 287 367 385
Cuts at: 4746 4763 6215 6301 6313 6659 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 598 543 392 385 367 346
287 135 86 67 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2857 3566 3765 513
Size: 552 939 853 709 199 3412
Fragments arranged by size:
3412 939 853 709 552 199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5925
Fragments arranged by size:
5925 683 56
BtsI GCAGTG_nn'
Cuts at: 4180 4974 4994 4180
Size: 794 20 5850
Fragments arranged by size:
5850 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6629
DraI TTT'AAA
Cuts at: 3900 3968 3900
Size: 68 6596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5671 809
Size: 507 4355 1802
Fragments arranged by size:
4355 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4111 5000 6629
Size: 54 1425 50 275 18 2281 889 1629
Cuts at: 6629 8
Size: 43
Fragments arranged by size:
2281 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4111 1537
Size: 275 2299 4090
Fragments arranged by size:
4090 2299 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 3393 4594 1353
Size: 873 1167 1201 3423
Fragments arranged by size:
3423 1201 1167 873
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5251 5625 5771 387
Size: 1064 138 3662 374 146 1280
Fragments arranged by size:
3662 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2474 4727 5324 6111 6379 1239
Size: 393 494 348 2253 597 787 268 1524
Fragments arranged by size:
2253 1524 787 597 494 393 348 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2578 2578
Size: 6664
EcoRI G'AATT_C
Cuts at: 2626 2801 3973 4351 2626
Size: 175 1172 378 4939
Fragments arranged by size:
4939 1172 378 175
EcoRV GAT'ATC
Cuts at: 2868 2868
Size: 6664
FspI TGC'GCA
Cuts at: 5170 5170
Size: 6664
HaeII r_GCGC'y
Cuts at: 1678 3423 5811 1678
Size: 1745 2388 2531
Fragments arranged by size:
2531 2388 1745
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2954 2986 3300 3332 5305
Size: 32 135 32 1382 32 314 32 1973
Cuts at: 5305 5337 5379 5411 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1973 1382 314 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2836 4008 4254 669
Size: 988 281 898 1172 246 3079
Fragments arranged by size:
3079 1172 988 898 281 246
HindIII A'AGCT_T
Cuts at: 748 3842 748
Size: 3094 3570
HpaI GTT'AAC
Cuts at: 4254 4254
Size: 6664
KpnI G_GTAC'C
Cuts at: 2086 3989 2086
Size: 1903 4761
MfeI C'AATT_G
Cuts at: 4263 4263
Size: 6664
MluI A'CGCG_T
Cuts at: 6540 6540
Size: 6664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2184 3505 5778 5962 1305
Size: 879 1321 2273 184 2007
Fragments arranged by size:
2273 2007 1321 879 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4842
Fragments arranged by size:
4842 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3810 3822 3924 4622 4981 5140 518
Size: 648 2644 12 102 698 359 159 2042
Fragments arranged by size:
2644 2042 698 648 359 159 102 12
MspA1I CmG'CkG
Cuts at: 1606 4080 4757 5905 6150 1606
Size: 2474 677 1148 245 2120
Fragments arranged by size:
2474 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2857 3566 513
Size: 552 939 853 709 3611
Fragments arranged by size:
3611 939 853 709 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6664
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6664
NotI GC'GGCC_GC
Cuts at: 4111 4111
Size: 6664
NspI r_CATG'y
Cuts at: 1132 1229 3628 4022 5567 1132
Size: 97 2399 394 1545 2229
Fragments arranged by size:
2399 2229 1545 394 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6664
PciI A'CATG_T
Cuts at: 3624 5563 3624
Size: 1939 4725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3400 1108
Size: 2292 4372
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 6246 6278 4733
Size: 32 1481 32 5119
Fragments arranged by size:
5119 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6664
PsiI TTA'TAA
Cuts at: 4234 4234
Size: 6664
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2385 2417 2385
Size: 32 6632
PstI C_TGCA'G
Cuts at: 830 4016 830
Size: 3186 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6664
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SapI GCTCTTCn'nnn_
Cuts at: 2226 2226
Size: 6664
SbfI CC_TGCA'GG
Cuts at: 4016 4016
Size: 6664
ScaI AGT'ACT
Cuts at: 4912 6624 4912
Size: 1712 4952
SfcI C'TryA_G
Cuts at: 826 1045 4012 5147 5828 6019 826
Size: 219 2967 1135 681 191 1471
Fragments arranged by size:
2967 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6664
SmaI CCC'GGG
Cuts at: 1872 3991 1872
Size: 2119 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2378 4768 5669 5931 820
Size: 197 926 435 2390 901 262 1553
Fragments arranged by size:
2390 1553 926 901 435 262 197
SnaBI TAC'GTA
Cuts at: 493 4455 493
Size: 3962 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6664
SphI G_CATG'C
Cuts at: 1132 4022 1132
Size: 2890 3774
SspI AAT'ATT
Cuts at: 5 52 4588 5
Size: 47 4536 2081
Fragments arranged by size:
4536 2081 47
StuI AGG'CCT
Cuts at: 1517 2384 3756 1517
Size: 867 1372 4425
Fragments arranged by size:
4425 1372 867
StyI C'CwwG_G
Cuts at: 513 1065 2004 2857 3566 3958 4103 513
Size: 552 939 853 709 392 145 3074
Fragments arranged by size:
3074 939 853 709 552 392 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4691 4708 4861 5046 5491 325
Size: 1255 3111 17 153 185 445 1498
Fragments arranged by size:
3111 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3661 3696 4910
Size: 274 80 33 51 1443 1684 35 1214
Cuts at: 4910 6622 96
Size: 1712 138
Fragments arranged by size:
1712 1684 1443 1214 274 138 80 51
35 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6624 6656 6624
Size: 32 6632
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6664
XbaI T'CTAG_A
Cuts at: 4000 4000
Size: 6664
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6664
XmaI C'CCGG_G
Cuts at: 1870 3989 1870
Size: 2119 4545
XmnI GAAnn'nnTTC
Cuts at: 2626 3511 3774 4793 2626
Size: 885 263 1019 4497
Fragments arranged by size:
4497 1019 885 263
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4472 276
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI