Restriction map for
pFN21AE2040
(Circular) (Six-base) MAPSORT of: pFN21AE2040.seq Check: 289 from: 1 to: 6658
pFN21AE2040 6658 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4468 278
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3546 3752 4001 1937
Size: 1609 206 249 4594
Fragments arranged by size:
4594 1609 249 206
Acc65I G'GTAC_C
Cuts at: 3979 3979
Size: 6658
AclI AA'CG_TT
Cuts at: 1563 4785 5158 1563
Size: 3222 373 3063
Fragments arranged by size:
3222 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2870 4721 6105 6373 1632
Size: 1238 1851 1384 268 1917
Fragments arranged by size:
1917 1851 1384 1238 268
AfeI AGC'GCT
Cuts at: 2492 2492
Size: 6658
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6461
AflIII A'CryG_T
Cuts at: 1203 3053 5557 6534 1203
Size: 1850 2504 977 1327
Fragments arranged by size:
2504 1850 1327 977
AgeI A'CCGG_T
Cuts at: 5489 5489
Size: 6658
AhdI GACnn_n'nnGTC
Cuts at: 5387 5387
Size: 6658
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6658
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6658
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2352 2384 2352
Size: 32 6626
AlwNI CAG_nnn'CTG
Cuts at: 4401 5973 4401
Size: 1572 5086
ApaI G_GGCC'C
Cuts at: 3049 3049
Size: 6658
ApaLI G'TGCA_C
Cuts at: 4715 5871 4715
Size: 1156 5502
ApoI r'AATT_y
Cuts at: 1493 3967 4193 4345 6454 1493
Size: 2474 226 152 2109 1697
Fragments arranged by size:
2474 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2754 3561 5212 160
Size: 872 1722 807 1651 1606
Fragments arranged by size:
1722 1651 1606 872 807
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
AvaI C'yCGr_G
Cuts at: 1870 1943 3983 1870
Size: 73 2040 4545
Fragments arranged by size:
4545 2040 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2772 2805 2772
Size: 33 6625
BamHI G'GATC_C
Cuts at: 2959 3988 4355 2959
Size: 1029 367 5262
Fragments arranged by size:
5262 1029 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3244 3979 5434 618
Size: 325 217 627 1457 735 1455 1842
Fragments arranged by size:
1842 1457 1455 735 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3049 3317 3977 1391
Size: 1658 268 660 4072
Fragments arranged by size:
4072 1658 660 268
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3488 928
Size: 971 1589 4098
Fragments arranged by size:
4098 1589 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4849 4883 1903
Size: 34 2912 34 3678
Fragments arranged by size:
3678 2912 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3529 4550 5760 3529
Size: 1021 1210 4427
Fragments arranged by size:
4427 1210 1021
BclI T'GATC_A
Cuts at: 1548 3715 3775 1548
Size: 2167 60 4431
Fragments arranged by size:
4431 2167 60
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5269 136
Size: 107 122 71 631 605 3597 1525
Fragments arranged by size:
3597 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6653 6653
Size: 6658
BlpI GC'TnA_GC
Cuts at: 4075 4075
Size: 6658
Bme1580I G_kGCm'C
Cuts at: 1790 3049 3247 4719 5875 1790
Size: 1259 198 1472 1156 2573
Fragments arranged by size:
2573 1472 1259 1156 198
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6658
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2390 4389 5347
Size: 416 53 83 399 24 973 1999 958
Cuts at: 5347 442
Size: 1753
Fragments arranged by size:
1999 1753 973 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3498 5318 1239
Size: 2259 1820 2579
Fragments arranged by size:
2579 2259 1820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3331 4783 5648 5946 3331
Size: 1452 865 298 4043
Fragments arranged by size:
4043 1452 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5321 882
Size: 617 3822 2219
Fragments arranged by size:
3822 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4449 493
Size: 711 3245 2702
Fragments arranged by size:
3245 2702 711
BsaBI GATnn'nnATC
Cuts at: 6652 6652
Size: 6658
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2216 4465 4847 5482
Size: 53 83 186 936 683 2249 382 635
Cuts at: 5482 275
Size: 1451
Fragments arranged by size:
2249 1451 936 683 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2404 2917 5091 5489 5763 5910 6321 2404
Size: 513 2174 398 274 147 411 2741
Fragments arranged by size:
2741 2174 513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2257 2287 2352 2382 3662
Size: 30 165 30 854 30 65 30 1280
Cuts at: 3662 3692 1178
Size: 30 4144
Fragments arranged by size:
4144 1280 854 165 65 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3748 1186
Size: 2562 4096
BseYI C'CCAG_C
Cuts at: 2741 5861 6357 2741
Size: 3120 496 3042
Fragments arranged by size:
3120 3042 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2558 3807 1163
Size: 573 822 1249 4014
Fragments arranged by size:
4014 1249 822 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4108 4869 5897 1540
Size: 275 186 2107 761 1028 2301
Fragments arranged by size:
2301 2107 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3317 4719 4804 5875 3317
Size: 1402 85 1071 4100
Fragments arranged by size:
4100 1402 1071 85
BsmI GAATG_Cn'
Cuts at: 4169 4262 4169
Size: 93 6565
BsmBI CGTCTCn'nnnn_
Cuts at: 3345 3741 3345
Size: 396 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3049 3247 3317 3977 4719 4804 5875
Size: 399 1259 198 70 660 742 85 1071
Cuts at: 5875 1391
Size: 2174
Fragments arranged by size:
2174 1259 1071 742 660 399 198 85
70
BspEI T'CCGG_A
Cuts at: 2404 2917 6321 2404
Size: 513 3404 2741
Fragments arranged by size:
3404 2741 513
BspHI T'CATG_A
Cuts at: 2211 4547 6277 2211
Size: 2336 1730 2592
Fragments arranged by size:
2592 2336 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4013 844
Size: 3169 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4545 1394
Size: 429 2722 3507
Fragments arranged by size:
3507 2722 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5153 5327 65
Size: 1183 3905 174 1396
Fragments arranged by size:
3905 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5302 5489 1700
Size: 110 3492 187 2869
Fragments arranged by size:
3492 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3200 96
Size: 3104 3554
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6658
BssSI C'ACGA_G
Cuts at: 4718 5730 4718
Size: 1012 5646
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6658
BstBI TT'CG_AA
Cuts at: 5462 5462
Size: 6658
BstEII G'GTnAC_C
Cuts at: 1184 2306 2699 1184
Size: 1122 393 5143
Fragments arranged by size:
5143 1122 393
BstYI r'GATC_y
Cuts at: 1972 2959 3988 4355 4740 4757 6209 6295 6307
Size: 987 1029 367 385 17 1452 86 12
Cuts at: 6307 6653 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1029 987 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3753 3753
Size: 6658
Bsu36I CC'TnA_GG
Cuts at: 2990 3866 2990
Size: 876 5782
BtgI C'CryG_G
Cuts at: 513 1065 2004 2556 2841 513
Size: 552 939 552 285 4330
Fragments arranged by size:
4330 939 552 552 285
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2304 487
Size: 683 56 1078 4841
Fragments arranged by size:
4841 1078 683 56
BtsI GCAGTG_nn'
Cuts at: 4174 4968 4988 4174
Size: 794 20 5844
Fragments arranged by size:
5844 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6658
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6623
DraI TTT'AAA
Cuts at: 3962 3962
Size: 6658
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5665 809
Size: 507 4349 1802
Fragments arranged by size:
4349 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4105 4994 6623
Size: 54 1425 50 275 18 2275 889 1629
Cuts at: 6623 8
Size: 43
Fragments arranged by size:
2275 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4105 1537
Size: 275 2293 4090
Fragments arranged by size:
4090 2293 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2346 2394 3408 3417 4588 1353
Size: 993 48 1014 9 1171 3423
Fragments arranged by size:
3423 1171 1014 993 48 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5245 5619 5765 387
Size: 1064 138 435 3221 374 146 1280
Fragments arranged by size:
3221 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 3315 3315
Size: 6658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2870 3498 4721 5318 6105 6373 1239
Size: 393 1238 628 1223 597 787 268 1524
Fragments arranged by size:
1524 1238 1223 787 628 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2348 3046 3441 2348
Size: 698 395 5565
Fragments arranged by size:
5565 698 395
EcoRI G'AATT_C
Cuts at: 3967 4345 3967
Size: 378 6280
FspI TGC'GCA
Cuts at: 5164 5164
Size: 6658
HaeII r_GCGC'y
Cuts at: 1678 2494 5805 1678
Size: 816 3311 2531
Fragments arranged by size:
3311 2531 816
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2944 2976 3389 3421 3430
Size: 32 135 32 1372 32 413 32 9
Cuts at: 3430 3462 3902 3934 5299 5331 5373 5405 1373
Size: 32 440 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1372 1365 440 413 135 42 32
32 32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2952 4002 4248 669
Size: 988 281 1014 1050 246 3079
Fragments arranged by size:
3079 1050 1014 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6658
HpaI GTT'AAC
Cuts at: 4248 4248
Size: 6658
KpnI G_GTAC'C
Cuts at: 3983 3983
Size: 6658
MfeI C'AATT_G
Cuts at: 4257 4257
Size: 6658
MluI A'CGCG_T
Cuts at: 6534 6534
Size: 6658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2797 3882 5772 5956 1305
Size: 1492 1085 1890 184 2007
Fragments arranged by size:
2007 1890 1492 1085 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4836
Fragments arranged by size:
4836 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3095 4616 4975 5134 518
Size: 648 1929 1521 359 159 2042
Fragments arranged by size:
2042 1929 1521 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3692 4074 4751 5899 6144 1606
Size: 2086 382 677 1148 245 2120
Fragments arranged by size:
2120 2086 1148 677 382 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2556 2841 513
Size: 552 939 552 285 4330
Fragments arranged by size:
4330 939 552 552 285
NdeI CA'TA_TG
Cuts at: 387 3151 387
Size: 2764 3894
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6658
NotI GC'GGCC_GC
Cuts at: 4105 4105
Size: 6658
NspI r_CATG'y
Cuts at: 1132 1229 3057 4016 5561 1132
Size: 97 1828 959 1545 2229
Fragments arranged by size:
2229 1828 1545 959 97
PacI TTA_AT'TAA
Cuts at: 2940 2940
Size: 6658
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6658
PciI A'CATG_T
Cuts at: 3053 5557 3053
Size: 2504 4154
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6658
PmeI GTTT'AAAC
Cuts at: 3962 3962
Size: 6658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2352 2384 4727 4759 6240 6272 2352
Size: 32 2343 32 1481 32 2738
Fragments arranged by size:
2738 2343 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2348 3441 2348
Size: 1093 5565
PshAI GACnn'nnGTC
Cuts at: 1613 2484 1613
Size: 871 5787
PsiI TTA'TAA
Cuts at: 4228 4228
Size: 6658
PspOMI G'GGCC_C
Cuts at: 3045 3045
Size: 6658
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2049 2081 2049
Size: 32 6626
PstI C_TGCA'G
Cuts at: 830 2224 2821 3697 4010 830
Size: 1394 597 876 313 3478
Fragments arranged by size:
3478 1394 876 597 313
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6658
SacI G_AGCT'C
Cuts at: 3317 3317
Size: 6658
SalI G'TCGA_C
Cuts at: 1936 4000 1936
Size: 2064 4594
SapI GCTCTTCn'nnn_
Cuts at: 2394 3417 2394
Size: 1023 5635
SbfI CC_TGCA'GG
Cuts at: 4010 4010
Size: 6658
ScaI AGT'ACT
Cuts at: 2067 3197 3799 4906 6618 2067
Size: 1130 602 1107 1712 2107
Fragments arranged by size:
2107 1712 1130 1107 602
SfcI C'TryA_G
Cuts at: 826 1045 2149 2185 2220 2817 3543 3583 3693
Size: 219 1104 36 35 597 726 40 110
Cuts at: 3693 4006 5141 5822 6013 826
Size: 313 1135 681 191 1471
Fragments arranged by size:
1471 1135 1104 726 681 597 313 219
191 110 40 36 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6658
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6658
SmaI CCC'GGG
Cuts at: 1872 3985 1872
Size: 2113 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3310 4762 5663 5925 820
Size: 197 926 1367 1452 901 262 1553
Fragments arranged by size:
1553 1452 1367 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4449 493
Size: 3956 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6658
SphI G_CATG'C
Cuts at: 1132 4016 1132
Size: 2884 3774
SspI AAT'ATT
Cuts at: 5 52 4582 5
Size: 47 4530 2081
Fragments arranged by size:
4530 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6658
StyI C'CwwG_G
Cuts at: 513 1065 2004 2556 2841 4097 513
Size: 552 939 552 285 1256 3074
Fragments arranged by size:
3074 1256 939 552 552 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2409 2668 3622 4685 4702 4855 5040
Size: 1255 829 259 954 1063 17 153 185
Cuts at: 5040 5485 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1063 954 829 445 259 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2065 2590 3195
Size: 274 80 33 51 1443 88 525 605
Cuts at: 3195 3200 3268 3797 4904 6616 96
Size: 5 68 529 1107 1712 138
Fragments arranged by size:
1712 1443 1107 605 529 525 274 138
88 80 68 51 33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2563 2595 3335 3367 6618 6650 2563
Size: 32 740 32 3251 32 2571
Fragments arranged by size:
3251 2571 740 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6658
XbaI T'CTAG_A
Cuts at: 2927 3994 2927
Size: 1067 5591
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6658
XmaI C'CCGG_G
Cuts at: 1870 3983 1870
Size: 2113 4545
XmnI GAAnn'nnTTC
Cuts at: 2836 4787 2836
Size: 1951 4707
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4466 276
Size: 53 83 186 936 2932 2468
Fragments arranged by size:
2932 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PacI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BstXI DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI
PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI