Restriction map for
pFN21AE2039
(Circular) (Six-base) MAPSORT of: pFN21AE2039.seq Check: 261 from: 1 to: 6649
pFN21AE2039 6649 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2613 3591 2613
Size: 978 5671
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2136 4459 278
Size: 53 83 186 936 600 2323 2468
Fragments arranged by size:
2468 2323 936 600 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3992 1937
Size: 2055 4594
Acc65I G'GTAC_C
Cuts at: 2979 3970 2979
Size: 991 5658
AclI AA'CG_TT
Cuts at: 1563 4776 5149 1563
Size: 3213 373 3063
Fragments arranged by size:
3213 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2659 2901 2930 4712 6096 6364 1632
Size: 1027 242 29 1782 1384 268 1917
Fragments arranged by size:
1917 1782 1384 1027 268 242 29
AfeI AGC'GCT
Cuts at: 2065 2065
Size: 6649
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6452
AflIII A'CryG_T
Cuts at: 1203 5548 6525 1203
Size: 4345 977 1327
Fragments arranged by size:
4345 1327 977
AgeI A'CCGG_T
Cuts at: 5480 5480
Size: 6649
AhdI GACnn_n'nnGTC
Cuts at: 5378 5378
Size: 6649
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6649
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3074 1184
Size: 1890 4759
AlwNI CAG_nnn'CTG
Cuts at: 4392 5964 4392
Size: 1572 5077
ApaI G_GGCC'C
Cuts at: 2840 2879 3856 2840
Size: 39 977 5633
Fragments arranged by size:
5633 977 39
ApaLI G'TGCA_C
Cuts at: 4706 5862 4706
Size: 1156 5493
ApoI r'AATT_y
Cuts at: 1493 3512 3958 4184 4336 6445 1493
Size: 2019 446 226 152 2109 1697
Fragments arranged by size:
2109 2019 1697 446 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5203 160
Size: 872 4171 1606
Fragments arranged by size:
4171 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
AvaI C'yCGr_G
Cuts at: 1870 1943 2688 2774 2926 3179 3187 3392 3749
Size: 73 745 86 152 253 8 205 357
Cuts at: 3749 3849 3920 3974 1870
Size: 100 71 54 4545
Fragments arranged by size:
4545 745 357 253 205 152 100 86
73 71 54 8
AvrII C'CTAG_G
Cuts at: 2974 2974
Size: 6649
BamHI G'GATC_C
Cuts at: 2656 3979 4346 2656
Size: 1323 367 4959
Fragments arranged by size:
4959 1323 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2483 2711 2979 3938 3970
Size: 325 217 627 696 228 268 959 32
Cuts at: 3970 5425 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 959 696 627 325 268 228
217 32
BanII G_rGCy'C
Cuts at: 1391 2152 2316 2334 2351 2775 2840 2879 2942
Size: 761 164 18 17 424 65 39 63
Cuts at: 2942 3080 3345 3564 3804 3856 3968 1391
Size: 138 265 219 240 52 112 4072
Fragments arranged by size:
4072 761 424 265 240 219 164 138
112 65 63 52 39 18 17
BbeI G_GCGC'C
Cuts at: 3942 3942
Size: 6649
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2554 928
Size: 971 655 5023
Fragments arranged by size:
5023 971 655
BbvCI CC'TCA_GC
Cuts at: 2411 2507 2984 3003 2411
Size: 96 477 19 6057
Fragments arranged by size:
6057 477 96 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2561 2595 3109 3143 3293 3327 4840
Size: 34 624 34 514 34 150 34 1513
Cuts at: 4840 4874 1903
Size: 34 3678
Fragments arranged by size:
3678 1513 624 514 150 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3107 4541 5751 3107
Size: 1434 1210 4005
Fragments arranged by size:
4005 1434 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6649
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2346 2874 5260
Size: 107 122 71 631 605 674 528 2386
Cuts at: 5260 136
Size: 1525
Fragments arranged by size:
2386 1525 674 631 605 528 122 107
71
BglII A'GATC_T
Cuts at: 6644 6644
Size: 6649
BlpI GC'TnA_GC
Cuts at: 3689 4066 3689
Size: 377 6272
Bme1580I G_kGCm'C
Cuts at: 1790 2486 2716 2840 2866 2879 3856 4710 5866
Size: 696 230 124 26 13 977 854 1156
Cuts at: 5866 1790
Size: 2573
Fragments arranged by size:
2573 1156 977 854 696 230 124 26
13
BmgBI CAC'GTC
Cuts at: 1318 3703 1318
Size: 2385 4264
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2155 2278 4380
Size: 416 53 83 399 24 738 123 2102
Cuts at: 4380 5338 442
Size: 958 1753
Fragments arranged by size:
2102 1753 958 738 416 399 123 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6649
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2108 2108
Size: 6649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2187 2188 2466 2636 2826 3929 5309 1239
Size: 948 1 278 170 190 1103 1380 2579
Fragments arranged by size:
2579 1380 1103 948 278 190 170 1
Bpu10I CC'TnA_GC
Cuts at: 2411 2507 2904 2984 3003 3081 2411
Size: 96 397 80 19 78 5979
Fragments arranged by size:
5979 397 96 80 78 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3644 4774 5639 5937 3644
Size: 1130 865 298 4356
Fragments arranged by size:
4356 1130 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3368 5312 882
Size: 617 1869 1944 2219
Fragments arranged by size:
2219 1944 1869 617
BsaAI yAC'GTr
Cuts at: 493 1204 2872 4440 493
Size: 711 1668 1568 2702
Fragments arranged by size:
2702 1668 1568 711
BsaBI GATnn'nnATC
Cuts at: 6643 6643
Size: 6649
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 3939 4456 4838
Size: 53 83 186 936 600 1806 517 382
Cuts at: 4838 5473 275
Size: 635 1451
Fragments arranged by size:
1806 1451 936 635 600 517 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2916 3483 5082 5480 5754 5901 6312 2916
Size: 567 1599 398 274 147 411 3253
Fragments arranged by size:
3253 1599 567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 2634 2664 3536
Size: 30 165 30 966 30 235 30 872
Cuts at: 3536 3566 1178
Size: 30 4261
Fragments arranged by size:
4261 966 872 235 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2246 2566 2867 2870 3020 3206 3938
Size: 988 72 320 301 3 150 186 732
Cuts at: 3938 3941 1186
Size: 3 3894
Fragments arranged by size:
3894 988 732 320 301 186 150 72
3 3
BseYI C'CCAG_C
Cuts at: 2333 2415 2809 2942 2988 3193 5852 6348 2333
Size: 82 394 133 46 205 2659 496 2634
Fragments arranged by size:
2659 2634 496 394 205 133 82 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2126 2346 2450 3563 3710 3788
Size: 573 303 87 220 104 1113 147 78
Cuts at: 3788 1163
Size: 4024
Fragments arranged by size:
4024 1113 573 303 220 147 104 87
78
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4099 4860 5888 1540
Size: 275 186 2098 761 1028 2301
Fragments arranged by size:
2301 2098 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3012 3345 4710 4795 5866 3012
Size: 333 1365 85 1071 3795
Fragments arranged by size:
3795 1365 1071 333 85
BsmI GAATG_Cn'
Cuts at: 4160 4253 4160
Size: 93 6556
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2316 2334 2351 2486 2716 2775
Size: 399 362 164 18 17 135 230 59
Cuts at: 2775 2840 2866 2879 2942 3012 3080 3345 3564
Size: 65 26 13 63 70 68 265 219
Cuts at: 3564 3804 3856 3968 4710 4795 5866 1391
Size: 240 52 112 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 362 265 240 230
219 164 135 112 85 70 68 65
63 59 52 26 18 17 13
BspEI T'CCGG_A
Cuts at: 6312 6312
Size: 6649
BspHI T'CATG_A
Cuts at: 4538 6268 4538
Size: 1730 4919
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2061 2526 2613 2887 3591 4004 844
Size: 1217 465 87 274 704 413 3489
Fragments arranged by size:
3489 1217 704 465 413 274 87
BsrBI CCG'CTC
Cuts at: 1394 1823 2397 2833 3091 4536 1394
Size: 429 574 436 258 1445 3507
Fragments arranged by size:
3507 1445 574 436 429 258
BsrDI GCAATG_nn'
Cuts at: 65 1248 5144 5318 65
Size: 1183 3896 174 1396
Fragments arranged by size:
3896 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 5293 5480 1700
Size: 110 205 3278 187 2869
Fragments arranged by size:
3278 2869 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6649
BssSI C'ACGA_G
Cuts at: 2317 3344 4709 5721 2317
Size: 1027 1365 1012 3245
Fragments arranged by size:
3245 1365 1027 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6649
BstBI TT'CG_AA
Cuts at: 5453 5453
Size: 6649
BstEII G'GTnAC_C
Cuts at: 1184 2434 1184
Size: 1250 5399
BstYI r'GATC_y
Cuts at: 1972 2656 2919 3654 3774 3912 3979 4346 4731
Size: 684 263 735 120 138 67 367 385
Cuts at: 4731 4748 6200 6286 6298 6644 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 735 684 385 367 346 263
138 120 86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2730 3413 2730
Size: 683 5966
BtgI C'CryG_G
Cuts at: 513 1065 2004 2476 2826 3876 513
Size: 552 939 472 350 1050 3286
Fragments arranged by size:
3286 1050 939 552 472 350
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2080 487
Size: 683 56 854 5056
Fragments arranged by size:
5056 854 683 56
BtsI GCAGTG_nn'
Cuts at: 2462 3068 4165 4959 4979 2462
Size: 606 1097 794 20 4132
Fragments arranged by size:
4132 1097 794 606 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6649
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6614
DraI TTT'AAA
Cuts at: 3953 3953
Size: 6649
DraIII CAC_nnn'GTG
Cuts at: 3766 3766
Size: 6649
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2132 2273 5656 809
Size: 507 816 141 3383 1802
Fragments arranged by size:
3383 1802 816 507 141
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2291 2526 2819
Size: 54 1425 50 275 18 461 235 293
Cuts at: 2819 4096 4985 6614 8
Size: 1277 889 1629 43
Fragments arranged by size:
1629 1425 1277 889 461 293 275 235
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4096 1537
Size: 275 2284 4090
Fragments arranged by size:
4090 2284 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2192 3357 4579 1353
Size: 839 1165 1222 3423
Fragments arranged by size:
3423 1222 1165 839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2058 2381 5236 5610 5756 387
Size: 1064 138 469 323 2855 374 146 1280
Fragments arranged by size:
2855 1280 1064 469 374 323 146 138
EcoICRI GAG'CTC
Cuts at: 3343 3343
Size: 6649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2188 2466 2636 2659 2826 2901
Size: 393 555 1 278 170 23 167 75
Cuts at: 2901 2930 3929 4712 5309 6096 6364 1239
Size: 29 999 783 597 787 268 1524
Fragments arranged by size:
1524 999 787 783 597 555 393 278
268 170 167 75 29 23 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2894 3920 2894
Size: 1026 5623
EcoO109I rG'GnC_Cy
Cuts at: 2813 2836 2837 2876 2899 3176 3721 3853 2813
Size: 23 1 39 23 277 545 132 5609
Fragments arranged by size:
5609 545 277 132 39 23 23 1
EcoRI G'AATT_C
Cuts at: 3512 3958 4336 3512
Size: 446 378 5825
Fragments arranged by size:
5825 446 378
EcoRV GAT'ATC
Cuts at: 3211 3211
Size: 6649
FspI TGC'GCA
Cuts at: 5155 5155
Size: 6649
HaeII r_GCGC'y
Cuts at: 1678 2067 3942 5796 1678
Size: 389 1875 1854 2531
Fragments arranged by size:
2531 1875 1854 389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2369 2401 2648
Size: 32 135 32 500 32 265 32 247
Cuts at: 2648 2680 2775 2807 3260 3292 3449 3481 5290
Size: 32 95 32 453 32 157 32 1809
Cuts at: 5290 5322 5364 5396 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1809 500 453 265 247 157 135
95 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3993 4239 669
Size: 988 281 2055 246 3079
Fragments arranged by size:
3079 2055 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6649
HpaI GTT'AAC
Cuts at: 4239 4239
Size: 6649
KasI G'GCGC_C
Cuts at: 3938 3938
Size: 6649
KpnI G_GTAC'C
Cuts at: 2983 3974 2983
Size: 991 5658
MfeI C'AATT_G
Cuts at: 4248 4248
Size: 6649
MluI A'CGCG_T
Cuts at: 6525 6525
Size: 6649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2613 2769 5763 5947 1305
Size: 1308 156 2994 184 2007
Fragments arranged by size:
2994 2007 1308 184 156
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 10
Size: 54 1425 343 696 4131
Fragments arranged by size:
4131 1425 696 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3626 4607 4966 5125 518
Size: 648 2460 981 359 159 2042
Fragments arranged by size:
2460 2042 981 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2337 2415 2478 2988 3002 3832 4065 4742
Size: 731 78 63 510 14 830 233 677
Cuts at: 4742 5890 6135 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 830 731 677 510 245 233
78 63 14
NaeI GCC'GGC
Cuts at: 2017 2017
Size: 6649
NarI GG'CG_CC
Cuts at: 3939 3939
Size: 6649
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5158
Fragments arranged by size:
5158 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6649
NgoMIV G'CCGG_C
Cuts at: 2015 2015
Size: 6649
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6649
NotI GC'GGCC_GC
Cuts at: 4096 4096
Size: 6649
NspI r_CATG'y
Cuts at: 1132 1229 3232 4007 5552 1132
Size: 97 2003 775 1545 2229
Fragments arranged by size:
2229 2003 1545 775 97
PasI CC'CwG_GG
Cuts at: 1348 2344 1348
Size: 996 5653
PciI A'CATG_T
Cuts at: 5548 5548
Size: 6649
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6649
PfoI T'CCnGG_A
Cuts at: 2652 3444 3522 3603 2652
Size: 792 78 81 5698
Fragments arranged by size:
5698 792 81 78
PmeI GTTT'AAAC
Cuts at: 3953 3953
Size: 6649
PmlI CAC'GTG
Cuts at: 2872 2872
Size: 6649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4718 4750 6231 6263 4718
Size: 32 1481 32 5104
Fragments arranged by size:
5104 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2899 3176 2899
Size: 277 6372
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6649
PsiI TTA'TAA
Cuts at: 4219 4219
Size: 6649
PspOMI G'GGCC_C
Cuts at: 2836 2875 3852 2836
Size: 39 977 5633
Fragments arranged by size:
5633 977 39
PstI C_TGCA'G
Cuts at: 830 2610 2721 2895 3063 4001 830
Size: 1780 111 174 168 938 3478
Fragments arranged by size:
3478 1780 938 174 168 111
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6649
PvuII CAG'CTG
Cuts at: 2337 2415 2988 3002 2337
Size: 78 573 14 5984
Fragments arranged by size:
5984 573 78 14
SacI G_AGCT'C
Cuts at: 3345 3345
Size: 6649
SacII CC_GC'GG
Cuts at: 2479 2479
Size: 6649
SalI G'TCGA_C
Cuts at: 1936 3991 1936
Size: 2055 4594
SanDI GG'GwC_CC
Cuts at: 3176 3176
Size: 6649
SbfI CC_TGCA'GG
Cuts at: 4001 4001
Size: 6649
ScaI AGT'ACT
Cuts at: 4897 6609 4897
Size: 1712 4937
SexAI A'CCwGG_T
Cuts at: 2424 2424
Size: 6649
SfcI C'TryA_G
Cuts at: 826 1045 2020 2154 2606 2717 2891 3059 3997
Size: 219 975 134 452 111 174 168 938
Cuts at: 3997 5132 5813 6004 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 975 938 681 452 219 191
174 168 134 111
SfoI GGC'GCC
Cuts at: 3940 3940
Size: 6649
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6649
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6649
SmaI CCC'GGG
Cuts at: 1872 3189 3851 3976 1872
Size: 1317 662 125 4545
Fragments arranged by size:
4545 1317 662 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3659 4753 5654 5916 820
Size: 197 926 1716 1094 901 262 1553
Fragments arranged by size:
1716 1553 1094 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4440 493
Size: 3947 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6649
SphI G_CATG'C
Cuts at: 1132 4007 1132
Size: 2875 3774
SspI AAT'ATT
Cuts at: 5 52 4573 5
Size: 47 4521 2081
Fragments arranged by size:
4521 2081 47
StuI AGG'CCT
Cuts at: 1517 2409 2960 1517
Size: 892 551 5206
Fragments arranged by size:
5206 892 551
StyI C'CwwG_G
Cuts at: 513 1065 2004 2974 4088 513
Size: 552 939 970 1114 3074
Fragments arranged by size:
3074 1114 970 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2097 2192 4676 4693 4846 5031 5476
Size: 1255 517 95 2484 17 153 185 445
Cuts at: 5476 325
Size: 1498
Fragments arranged by size:
2484 1498 1255 517 445 185 153 95
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4895 6607 96
Size: 274 80 33 51 1443 2918 1712 138
Fragments arranged by size:
2918 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2454 2486 2634 2666 3536 3568 6609 6641 2454
Size: 32 148 32 870 32 3041 32 2462
Fragments arranged by size:
3041 2462 870 148 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6649
XbaI T'CTAG_A
Cuts at: 3985 3985
Size: 6649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2412 3607 2412
Size: 1195 5454
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6649
XmaI C'CCGG_G
Cuts at: 1870 3187 3849 3974 1870
Size: 1317 662 125 4545
Fragments arranged by size:
4545 1317 662 125
XmnI GAAnn'nnTTC
Cuts at: 4778 4778
Size: 6649
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2134 4457 276
Size: 53 83 186 936 600 2323 2468
Fragments arranged by size:
2468 2323 936 600 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BsmBI BstXI BstZ17I
FalI FseI FspAI NruI NsiI PacI PspXI PsrI
RsrII SapI SfiI SrfI SwaI