Restriction map for
pFN21AE2033
(Circular) (Six-base) MAPSORT of: pFN21AE2033.seq Check: 3547 from: 1 to: 6577
pFN21AE2033 6577 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2682 2682
Size: 6577
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4387 278
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2384 3920 1937
Size: 447 1536 4594
Fragments arranged by size:
4594 1536 447
Acc65I G'GTAC_C
Cuts at: 3898 3898
Size: 6577
AclI AA'CG_TT
Cuts at: 1563 4704 5077 1563
Size: 3141 373 3063
Fragments arranged by size:
3141 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3426 4640 6024 6292 1632
Size: 1794 1214 1384 268 1917
Fragments arranged by size:
1917 1794 1384 1214 268
AfeI AGC'GCT
Cuts at: 3625 3625
Size: 6577
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6380
AflIII A'CryG_T
Cuts at: 1203 5476 6453 1203
Size: 4273 977 1327
Fragments arranged by size:
4273 1327 977
AgeI A'CCGG_T
Cuts at: 5408 5408
Size: 6577
AhdI GACnn_n'nnGTC
Cuts at: 5306 5306
Size: 6577
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6577
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6577
AlwNI CAG_nnn'CTG
Cuts at: 3118 3199 3662 3703 4320 5892 3118
Size: 81 463 41 617 1572 3803
Fragments arranged by size:
3803 1572 617 463 81 41
ApaI G_GGCC'C
Cuts at: 3549 3549
Size: 6577
ApaLI G'TGCA_C
Cuts at: 4634 5790 4634
Size: 1156 5421
ApoI r'AATT_y
Cuts at: 1493 3886 4112 4264 6373 1493
Size: 2393 226 152 2109 1697
Fragments arranged by size:
2393 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5131 160
Size: 872 4099 1606
Fragments arranged by size:
4099 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
AvaI C'yCGr_G
Cuts at: 1870 1943 2150 3902 1870
Size: 73 207 1752 4545
Fragments arranged by size:
4545 1752 207 73
BamHI G'GATC_C
Cuts at: 3907 4274 3907
Size: 367 6210
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2146 2965 3169 3898 5353
Size: 325 217 627 359 819 204 729 1455
Cuts at: 5353 618
Size: 1842
Fragments arranged by size:
1842 1455 819 729 627 359 325 217
204
BanII G_rGCy'C
Cuts at: 1391 2486 2505 3549 3866 3896 1391
Size: 1095 19 1044 317 30 4072
Fragments arranged by size:
4072 1095 1044 317 30 19
BbeI G_GCGC'C
Cuts at: 3173 3173
Size: 6577
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2123 928
Size: 971 224 5382
Fragments arranged by size:
5382 971 224
BbvCI CC'TCA_GC
Cuts at: 3264 3264
Size: 6577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3821 3855 4768 4802 1903
Size: 34 1884 34 913 34 3678
Fragments arranged by size:
3678 1884 913 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4469 5679 4469
Size: 1210 5367
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6577
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3480 3507 5188
Size: 107 122 71 631 605 1808 27 1681
Cuts at: 5188 136
Size: 1525
Fragments arranged by size:
1808 1681 1525 631 605 122 107 71
27
BglII A'GATC_T
Cuts at: 6572 6572
Size: 6577
BlpI GC'TnA_GC
Cuts at: 2088 3200 3994 2088
Size: 1112 794 4671
Fragments arranged by size:
4671 1112 794
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2968 3549 4638 5794 1790
Size: 361 817 581 1089 1156 2573
Fragments arranged by size:
2573 1156 1089 817 581 361
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6577
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3063 3479 4308
Size: 416 53 83 399 24 1646 416 829
Cuts at: 4308 5266 442
Size: 958 1753
Fragments arranged by size:
1753 1646 958 829 416 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6577
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 3450 2176
Size: 1274 5303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2489 3420 3729 5237 1239
Size: 1250 931 309 1508 2579
Fragments arranged by size:
2579 1508 1250 931 309
Bpu10I CC'TnA_GC
Cuts at: 2465 3264 2465
Size: 799 5778
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2500 4702 5567 5865 2500
Size: 2202 865 298 3212
Fragments arranged by size:
3212 2202 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5240 882
Size: 617 3741 2219
Fragments arranged by size:
3741 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4368 493
Size: 711 3164 2702
Fragments arranged by size:
3164 2702 711
BsaBI GATnn'nnATC
Cuts at: 2549 6571 2549
Size: 4022 2555
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2381 3170 4384 4766
Size: 53 83 186 936 848 789 1214 382
Cuts at: 4766 5401 275
Size: 635 1451
Fragments arranged by size:
1451 1214 936 848 789 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5010 5408 5682 5829 6240 5010
Size: 398 274 147 411 5347
Fragments arranged by size:
5347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6352
Fragments arranged by size:
6352 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2893 1186
Size: 964 743 4870
Fragments arranged by size:
4870 964 743
BseYI C'CCAG_C
Cuts at: 3144 3295 3457 3859 3867 5780 6276 3144
Size: 151 162 402 8 1913 496 3445
Fragments arranged by size:
3445 1913 496 402 162 151 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3207 3612 3716 1163
Size: 573 1471 405 104 4024
Fragments arranged by size:
4024 1471 573 405 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4027 4788 5816 1540
Size: 275 186 2026 761 1028 2301
Fragments arranged by size:
2301 2026 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2486 2505 4638 4723 5794 2486
Size: 19 2133 85 1071 3269
Fragments arranged by size:
3269 2133 1071 85 19
BsmI GAATG_Cn'
Cuts at: 2851 4088 4181 2851
Size: 1237 93 5247
Fragments arranged by size:
5247 1237 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2486 2505 2968 3549 3866 3896
Size: 399 361 335 19 463 581 317 30
Cuts at: 3896 4638 4723 5794 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 581 463 399 361 335
317 85 30 19
BspEI T'CCGG_A
Cuts at: 6240 6240
Size: 6577
BspHI T'CATG_A
Cuts at: 4466 6196 4466
Size: 1730 4847
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2682 3932 844
Size: 1838 1250 3489
Fragments arranged by size:
3489 1838 1250
BsrBI CCG'CTC
Cuts at: 1394 1823 4464 1394
Size: 429 2641 3507
Fragments arranged by size:
3507 2641 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2359 3052 3363 3783 5072 5246 65
Size: 1183 1111 693 311 420 1289 174 1396
Fragments arranged by size:
1396 1289 1183 1111 693 420 311 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3587 5221 5408 1700
Size: 110 1777 1634 187 2869
Fragments arranged by size:
2869 1777 1634 187 110
BsrGI T'GTAC_A
Cuts at: 96 2817 96
Size: 2721 3856
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6577
BssSI C'ACGA_G
Cuts at: 3643 4637 5649 3643
Size: 994 1012 4571
Fragments arranged by size:
4571 1012 994
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3118 3313 3694 794
Size: 2324 195 381 3677
Fragments arranged by size:
3677 2324 381 195
BstBI TT'CG_AA
Cuts at: 2189 2591 5381 2189
Size: 402 2790 3385
Fragments arranged by size:
3385 2790 402
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6577
BstYI r'GATC_y
Cuts at: 1972 3375 3907 4274 4659 4676 6128 6214 6226
Size: 1403 532 367 385 17 1452 86 12
Cuts at: 6226 6572 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1403 532 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2328 2328
Size: 6577
BtgI C'CryG_G
Cuts at: 513 1065 2004 2047 2358 2886 2960 3603 513
Size: 552 939 43 311 528 74 643 3487
Fragments arranged by size:
3487 939 643 552 528 311 74 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5838
Fragments arranged by size:
5838 683 56
BtsI GCAGTG_nn'
Cuts at: 4093 4887 4907 4093
Size: 794 20 5763
Fragments arranged by size:
5763 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6577
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6542
DraI TTT'AAA
Cuts at: 3881 3881
Size: 6577
DraIII CAC_nnn'GTG
Cuts at: 3635 3635
Size: 6577
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5584 809
Size: 507 4268 1802
Fragments arranged by size:
4268 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2532 3284 4024
Size: 54 1425 50 275 18 702 752 740
Cuts at: 4024 4913 6542 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 752 740 702 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4024 1537
Size: 275 2212 4090
Fragments arranged by size:
4090 2212 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4507 1353
Size: 3154 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5164 5538 5684 387
Size: 1064 138 3575 374 146 1280
Fragments arranged by size:
3575 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2484 2503 2484
Size: 19 6558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2489 3420 3426 3729 4640 5237 6024
Size: 393 857 931 6 303 911 597 787
Cuts at: 6024 6292 1239
Size: 268 1524
Fragments arranged by size:
1524 931 911 857 787 597 393 303
268 6
EcoO109I rG'GnC_Cy
Cuts at: 3473 3500 3509 3536 3554 3608 3700 3811 3473
Size: 27 9 27 18 54 92 111 6239
Fragments arranged by size:
6239 111 92 54 27 27 18 9
EcoRI G'AATT_C
Cuts at: 3886 4264 3886
Size: 378 6199
FspI TGC'GCA
Cuts at: 5083 5083
Size: 6577
HaeII r_GCGC'y
Cuts at: 1678 3173 3627 5724 1678
Size: 1495 454 2097 2531
Fragments arranged by size:
2531 2097 1495 454
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2737 2769 3193 3225 3304
Size: 32 135 32 1165 32 424 32 79
Cuts at: 3304 3317 3336 3349 3819 3851 5218 5250 5292
Size: 13 19 13 470 32 1367 32 42
Cuts at: 5292 5324 1373
Size: 32 2626
Fragments arranged by size:
2626 1367 1165 470 424 135 79 42
32 32 32 32 32 32 32 19
13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2385 3921 4167 669
Size: 988 281 447 1536 246 3079
Fragments arranged by size:
3079 1536 988 447 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6577
HpaI GTT'AAC
Cuts at: 4167 4167
Size: 6577
KasI G'GCGC_C
Cuts at: 3169 3169
Size: 6577
KpnI G_GTAC'C
Cuts at: 3902 3902
Size: 6577
MfeI C'AATT_G
Cuts at: 2642 4176 2642
Size: 1534 5043
MluI A'CGCG_T
Cuts at: 6453 6453
Size: 6577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5691 5875 1305
Size: 4386 184 2007
Fragments arranged by size:
4386 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 10
Size: 54 1425 343 702 4053
Fragments arranged by size:
4053 1425 702 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4535 4894 5053 518
Size: 648 3369 359 159 2042
Fragments arranged by size:
3369 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2049 2360 3148 3247 3268 3727 3993
Size: 422 21 311 788 99 21 459 266
Cuts at: 3993 4670 5818 6063 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 788 677 459 422 311 266
245 99 21 21
NarI GG'CG_CC
Cuts at: 3170 3170
Size: 6577
NcoI C'CATG_G
Cuts at: 513 1065 2004 2960 513
Size: 552 939 956 4130
Fragments arranged by size:
4130 956 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6577
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6577
NotI GC'GGCC_GC
Cuts at: 4024 4024
Size: 6577
NruI TCG'CGA
Cuts at: 2943 2943
Size: 6577
NspI r_CATG'y
Cuts at: 1132 1229 2587 2818 3112 3935 5480 1132
Size: 97 1358 231 294 823 1545 2229
Fragments arranged by size:
2229 1545 1358 823 294 231 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6577
PciI A'CATG_T
Cuts at: 5476 5476
Size: 6577
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2560 2961 1108
Size: 1452 401 4724
Fragments arranged by size:
4724 1452 401
PmeI GTTT'AAAC
Cuts at: 3881 3881
Size: 6577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4646 4678 6159 6191 4646
Size: 32 1481 32 5032
Fragments arranged by size:
5032 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3554 3554
Size: 6577
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6577
PsiI TTA'TAA
Cuts at: 4147 4147
Size: 6577
PspOMI G'GGCC_C
Cuts at: 3545 3545
Size: 6577
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2356 2388 2356
Size: 32 6545
PstI C_TGCA'G
Cuts at: 830 3273 3309 3571 3929 830
Size: 2443 36 262 358 3478
Fragments arranged by size:
3478 2443 358 262 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6577
PvuII CAG'CTG
Cuts at: 3148 3268 3727 3148
Size: 120 459 5998
Fragments arranged by size:
5998 459 120
SacI G_AGCT'C
Cuts at: 2486 2505 2486
Size: 19 6558
SacII CC_GC'GG
Cuts at: 2050 2361 2050
Size: 311 6266
SalI G'TCGA_C
Cuts at: 1936 2383 3919 1936
Size: 447 1536 4594
Fragments arranged by size:
4594 1536 447
SbfI CC_TGCA'GG
Cuts at: 3929 3929
Size: 6577
ScaI AGT'ACT
Cuts at: 4825 6537 4825
Size: 1712 4865
SfcI C'TryA_G
Cuts at: 826 1045 3269 3305 3389 3468 3477 3513 3567
Size: 219 2224 36 84 79 9 36 54
Cuts at: 3567 3925 5060 5741 5932 826
Size: 358 1135 681 191 1471
Fragments arranged by size:
2224 1471 1135 681 358 219 191 84
79 54 36 36 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3480 3507 3480
Size: 27 6550
SfoI GGC'GCC
Cuts at: 3171 3171
Size: 6577
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6577
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6577
SmaI CCC'GGG
Cuts at: 1872 3904 1872
Size: 2032 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2479 4681 5582 5844 820
Size: 197 926 536 2202 901 262 1553
Fragments arranged by size:
2202 1553 926 901 536 262 197
SnaBI TAC'GTA
Cuts at: 493 4368 493
Size: 3875 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6577
SphI G_CATG'C
Cuts at: 1132 2587 3112 3935 1132
Size: 1455 525 823 3774
Fragments arranged by size:
3774 1455 823 525
SspI AAT'ATT
Cuts at: 5 52 4501 5
Size: 47 4449 2081
Fragments arranged by size:
4449 2081 47
StuI AGG'CCT
Cuts at: 1517 2463 1517
Size: 946 5631
StyI C'CwwG_G
Cuts at: 513 1065 2004 2065 2960 4016 513
Size: 552 939 61 895 1056 3074
Fragments arranged by size:
3074 1056 939 895 552 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4604 4621 4774 4959 5404 325
Size: 1255 3024 17 153 185 445 1498
Fragments arranged by size:
3024 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2817 4823 6535
Size: 274 80 33 51 1443 840 2006 1712
Cuts at: 6535 96
Size: 138
Fragments arranged by size:
2006 1712 1443 840 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6537 6569 6537
Size: 32 6545
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6577
XbaI T'CTAG_A
Cuts at: 2776 3913 2776
Size: 1137 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6577
XmaI C'CCGG_G
Cuts at: 1870 3902 1870
Size: 2032 4545
XmnI GAAnn'nnTTC
Cuts at: 2195 4706 2195
Size: 2511 4066
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4385 276
Size: 53 83 186 936 2851 2468
Fragments arranged by size:
2851 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NruI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV
NsiI PacI PfoI PmlI PspXI RsrII SanDI SapI
SexAI SrfI SwaI XcmI