Restriction map for
pFN21AE2032
(Circular) (Six-base) MAPSORT of: pFN21AE2032.seq Check: 2595 from: 1 to: 6571
pFN21AE2032 6571 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3127 3565 3127
Size: 438 6133
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4381 278
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3914 1937
Size: 1977 4594
Acc65I G'GTAC_C
Cuts at: 3892 3892
Size: 6571
AclI AA'CG_TT
Cuts at: 1563 3546 4698 5071 1563
Size: 1983 1152 373 3063
Fragments arranged by size:
3063 1983 1152 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3409 3428 3545 3851 4634 6018 6286 1632
Size: 1777 19 117 306 783 1384 268 1917
Fragments arranged by size:
1917 1777 1384 783 306 268 117 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6374
AflIII A'CryG_T
Cuts at: 1203 3645 3817 5470 6447 1203
Size: 2442 172 1653 977 1327
Fragments arranged by size:
2442 1653 1327 977 172
AgeI A'CCGG_T
Cuts at: 5402 5402
Size: 6571
AhdI GACnn_n'nnGTC
Cuts at: 3719 5300 3719
Size: 1581 4990
AleI CACnn'nnGTG
Cuts at: 1166 3818 1166
Size: 2652 3919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6571
AlwNI CAG_nnn'CTG
Cuts at: 3169 4314 5886 3169
Size: 1145 1572 3854
Fragments arranged by size:
3854 1572 1145
ApaLI G'TGCA_C
Cuts at: 2069 4628 5784 2069
Size: 2559 1156 2856
Fragments arranged by size:
2856 2559 1156
ApoI r'AATT_y
Cuts at: 1493 2467 3880 4106 4258 6367 1493
Size: 974 1413 226 152 2109 1697
Fragments arranged by size:
2109 1697 1413 974 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5125 160
Size: 872 4093 1606
Fragments arranged by size:
4093 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
AvaI C'yCGr_G
Cuts at: 1870 1943 2292 2995 3896 1870
Size: 73 349 703 901 4545
Fragments arranged by size:
4545 901 703 349 73
BamHI G'GATC_C
Cuts at: 3627 3901 4268 3627
Size: 274 367 5930
Fragments arranged by size:
5930 367 274
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2673 3892 5347 618
Size: 325 217 627 886 1219 1455 1842
Fragments arranged by size:
1842 1455 1219 886 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2700 3069 3107 3734 3890 1391
Size: 1309 369 38 627 156 4072
Fragments arranged by size:
4072 1309 627 369 156 38
BbeI G_GCGC'C
Cuts at: 2677 2677
Size: 6571
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5600
BbvCI CC'TCA_GC
Cuts at: 2097 2331 2097
Size: 234 6337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4762 4796 1903
Size: 34 2825 34 3678
Fragments arranged by size:
3678 2825 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3485 3834 4463 5673 3485
Size: 349 629 1210 4383
Fragments arranged by size:
4383 1210 629 349
BclI T'GATC_A
Cuts at: 1548 3856 1548
Size: 2308 4263
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2219 5182 136
Size: 107 122 71 631 605 547 2963 1525
Fragments arranged by size:
2963 1525 631 605 547 122 107 71
BglII A'GATC_T
Cuts at: 3342 6566 3342
Size: 3224 3347
BlpI GC'TnA_GC
Cuts at: 3988 3988
Size: 6571
Bme1580I G_kGCm'C
Cuts at: 1790 2073 4632 5788 1790
Size: 283 2559 1156 2573
Fragments arranged by size:
2573 2559 1156 283
BmgBI CAC'GTC
Cuts at: 1318 2014 2196 3534 1318
Size: 696 182 1338 4355
Fragments arranged by size:
4355 1338 696 182
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3558 4302 5260
Size: 416 53 83 399 24 2141 744 958
Cuts at: 5260 442
Size: 1753
Fragments arranged by size:
2141 1753 958 744 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6571
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2307 2307
Size: 6571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2192 2246 2593 3065 3467 5231 1239
Size: 863 90 54 347 472 402 1764 2579
Fragments arranged by size:
2579 1764 863 472 402 347 90 54
Bpu10I CC'TnA_GC
Cuts at: 2097 2331 2738 2097
Size: 234 407 5930
Fragments arranged by size:
5930 407 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4696 5561 5859 4696
Size: 865 298 5408
Fragments arranged by size:
5408 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5234 882
Size: 617 3735 2219
Fragments arranged by size:
3735 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4362 493
Size: 711 3158 2702
Fragments arranged by size:
3158 2702 711
BsaBI GATnn'nnATC
Cuts at: 3347 3632 6565 3347
Size: 285 2933 3353
Fragments arranged by size:
3353 2933 285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2288 2674 4378 4760
Size: 53 83 186 936 755 386 1704 382
Cuts at: 4760 5395 275
Size: 635 1451
Fragments arranged by size:
1704 1451 936 755 635 386 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3150 5004 5402 5676 5823 6234 3150
Size: 1854 398 274 147 411 3487
Fragments arranged by size:
3487 1854 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2165 2180 2195 2210 2682
Size: 30 165 30 762 15 15 15 472
Cuts at: 2682 2712 2804 2834 3158 3188 1178
Size: 30 92 30 324 30 4561
Fragments arranged by size:
4561 762 472 324 165 92 30 30
30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2085 2165 2204 2222 2416 2548 2825 2894
Size: 899 80 39 18 194 132 277 69
Cuts at: 2894 2906 2909 1186
Size: 12 3 4848
Fragments arranged by size:
4848 899 277 194 132 80 69 39
18 12 3
BseYI C'CCAG_C
Cuts at: 3201 3513 5774 6270 3201
Size: 312 2261 496 3502
Fragments arranged by size:
3502 2261 496 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 2789 1163
Size: 573 482 571 4945
Fragments arranged by size:
4945 573 571 482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2111 3605 4021 4782 5810 1540
Size: 275 186 110 1494 416 761 1028 2301
Fragments arranged by size:
2301 1494 1028 761 416 275 186 110
BsiHKAI G_wGCw'C
Cuts at: 2073 3069 4632 4717 5788 2073
Size: 996 1563 85 1071 2856
Fragments arranged by size:
2856 1563 1071 996 85
BsmI GAATG_Cn'
Cuts at: 2549 4082 4175 2549
Size: 1533 93 4945
Fragments arranged by size:
4945 1533 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2186 3458 3524 3772 2186
Size: 1272 66 248 4985
Fragments arranged by size:
4985 1272 248 66
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2700 3069 3107 3734 3890 4632
Size: 399 283 627 369 38 627 156 742
Cuts at: 4632 4717 5788 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 627 627 399 369 283
156 85 38
BspEI T'CCGG_A
Cuts at: 3150 6234 3150
Size: 3084 3487
BspHI T'CATG_A
Cuts at: 3723 4460 6190 3723
Size: 737 1730 4104
Fragments arranged by size:
4104 1730 737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3127 3565 3594 3926 844
Size: 2283 438 29 332 3489
Fragments arranged by size:
3489 2283 438 332 29
BsrBI CCG'CTC
Cuts at: 1394 1823 3601 4458 1394
Size: 429 1778 857 3507
Fragments arranged by size:
3507 1778 857 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3390 5066 5240 65
Size: 1183 2142 1676 174 1396
Fragments arranged by size:
2142 1676 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3604 5215 5402 1700
Size: 110 1794 1611 187 2869
Fragments arranged by size:
2869 1794 1611 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6571
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6571
BssSI C'ACGA_G
Cuts at: 4631 5643 4631
Size: 1012 5559
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2388 3142 794
Size: 1594 754 4223
Fragments arranged by size:
4223 1594 754
BstBI TT'CG_AA
Cuts at: 5375 5375
Size: 6571
BstEII G'GTnAC_C
Cuts at: 1184 2683 1184
Size: 1499 5072
BstYI r'GATC_y
Cuts at: 1972 3342 3627 3901 4268 4653 4670 6122 6208
Size: 1370 285 274 367 385 17 1452 86
Cuts at: 6208 6220 6566 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1370 385 367 346 285 274
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2235 2496 2886 513
Size: 552 939 231 261 390 4198
Fragments arranged by size:
4198 939 552 390 261 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2600 487
Size: 683 56 1374 4458
Fragments arranged by size:
4458 1374 683 56
BtsI GCAGTG_nn'
Cuts at: 2645 3220 4087 4881 4901 2645
Size: 575 867 794 20 4315
Fragments arranged by size:
4315 867 794 575 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6571
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6536
DraI TTT'AAA
Cuts at: 3875 3875
Size: 6571
DraIII CAC_nnn'GTG
Cuts at: 3445 3445
Size: 6571
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3203 5578 809
Size: 507 1887 2375 1802
Fragments arranged by size:
2375 1887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2563 2832 3602
Size: 54 1425 50 275 18 733 269 770
Cuts at: 3602 4018 4907 6536 8
Size: 416 889 1629 43
Fragments arranged by size:
1629 1425 889 770 733 416 275 269
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3602 4018 1537
Size: 275 1790 416 4090
Fragments arranged by size:
4090 1790 416 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3149 3722 4501 1353
Size: 1796 573 779 3423
Fragments arranged by size:
3423 1796 779 573
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3815 5158 5532 5678 387
Size: 1064 138 2226 1343 374 146 1280
Fragments arranged by size:
2226 1343 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3067 3067
Size: 6571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2192 2246 2593 3065 3409 3428
Size: 393 470 90 54 347 472 344 19
Cuts at: 3428 3467 3545 3851 4634 5231 6018 6286 1239
Size: 39 78 306 783 597 787 268 1524
Fragments arranged by size:
1524 787 783 597 472 470 393 347
344 306 268 90 78 54 39 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3672 3672
Size: 6571
EcoO109I rG'GnC_Cy
Cuts at: 2501 2734 2985 3205 3713 2501
Size: 233 251 220 508 5359
Fragments arranged by size:
5359 508 251 233 220
EcoRI G'AATT_C
Cuts at: 3880 4258 3880
Size: 378 6193
EcoRV GAT'ATC
Cuts at: 3327 3327
Size: 6571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3751 3751
Size: 6571
FspI TGC'GCA
Cuts at: 2159 3278 5077 2159
Size: 1119 1799 3653
Fragments arranged by size:
3653 1799 1119
FspAI rTGC'GCAy
Cuts at: 3278 3278
Size: 6571
HaeII r_GCGC'y
Cuts at: 1678 2677 5718 1678
Size: 999 3041 2531
Fragments arranged by size:
3041 2531 999
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2744 2776 2906 2938 3023
Size: 32 135 32 1172 32 130 32 85
Cuts at: 3023 3055 3705 3737 5212 5244 5286 5318 1373
Size: 32 650 32 1475 32 42 32 2626
Fragments arranged by size:
2626 1475 1172 650 135 130 85 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3915 4161 669
Size: 988 281 1977 246 3079
Fragments arranged by size:
3079 1977 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2300 748
Size: 1552 5019
HpaI GTT'AAC
Cuts at: 4161 4161
Size: 6571
KasI G'GCGC_C
Cuts at: 2673 2673
Size: 6571
KpnI G_GTAC'C
Cuts at: 3896 3896
Size: 6571
MfeI C'AATT_G
Cuts at: 4170 4170
Size: 6571
MluI A'CGCG_T
Cuts at: 6447 6447
Size: 6571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3315 5685 5869 1305
Size: 2010 2370 184 2007
Fragments arranged by size:
2370 2010 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2565 10
Size: 54 1425 343 733 4016
Fragments arranged by size:
4016 1425 733 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3818 4529 4888 5047 518
Size: 648 2652 711 359 159 2042
Fragments arranged by size:
2652 2042 711 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2122 2765 2773 3201 3747 3987 4664
Size: 423 93 643 8 428 546 240 677
Cuts at: 4664 5812 6057 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 643 546 428 423 245
240 93 8
NarI GG'CG_CC
Cuts at: 2674 2674
Size: 6571
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5080
Fragments arranged by size:
5080 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6571
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6571
NotI GC'GGCC_GC
Cuts at: 4018 4018
Size: 6571
NspI r_CATG'y
Cuts at: 1132 1229 2084 2313 2363 3127 3649 3821 3929
Size: 97 855 229 50 764 522 172 108
Cuts at: 3929 5474 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 855 764 522 229 172 108
97 50
PasI CC'CwG_GG
Cuts at: 1348 3240 1348
Size: 1892 4679
PciI A'CATG_T
Cuts at: 3645 3817 5470 3645
Size: 172 1653 4746
Fragments arranged by size:
4746 1653 172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6571
PfoI T'CCnGG_A
Cuts at: 3069 3069
Size: 6571
PmeI GTTT'AAAC
Cuts at: 3875 3875
Size: 6571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6153 6185 4640
Size: 32 1481 32 5026
Fragments arranged by size:
5026 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2501 2985 3205 3713 2501
Size: 484 220 508 5359
Fragments arranged by size:
5359 508 484 220
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6571
PsiI TTA'TAA
Cuts at: 4141 4141
Size: 6571
PstI C_TGCA'G
Cuts at: 830 3576 3923 830
Size: 2746 347 3478
Fragments arranged by size:
3478 2746 347
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6571
PvuII CAG'CTG
Cuts at: 2029 2765 2773 2029
Size: 736 8 5827
Fragments arranged by size:
5827 736 8
SacI G_AGCT'C
Cuts at: 3069 3069
Size: 6571
SalI G'TCGA_C
Cuts at: 1936 3913 1936
Size: 1977 4594
SanDI GG'GwC_CC
Cuts at: 2501 3713 2501
Size: 1212 5359
SapI GCTCTTCn'nnn_
Cuts at: 3722 3722
Size: 6571
SbfI CC_TGCA'GG
Cuts at: 3576 3923 3576
Size: 347 6224
ScaI AGT'ACT
Cuts at: 4819 6531 4819
Size: 1712 4859
SexAI A'CCwGG_T
Cuts at: 3299 3299
Size: 6571
SfcI C'TryA_G
Cuts at: 826 1045 2459 2517 2580 3572 3919 5054 5735
Size: 219 1414 58 63 992 347 1135 681
Cuts at: 5735 5926 826
Size: 191 1471
Fragments arranged by size:
1471 1414 1135 992 681 347 219 191
63 58
SfoI GGC'GCC
Cuts at: 2675 2675
Size: 6571
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6571
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6571
SmaI CCC'GGG
Cuts at: 1872 3898 1872
Size: 2026 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2292 4675 5576 5838 820
Size: 197 926 349 2383 901 262 1553
Fragments arranged by size:
2383 1553 926 901 349 262 197
SnaBI TAC'GTA
Cuts at: 493 4362 493
Size: 3869 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6571
SphI G_CATG'C
Cuts at: 1132 3929 1132
Size: 2797 3774
SspI AAT'ATT
Cuts at: 5 52 4495 5
Size: 47 4443 2081
Fragments arranged by size:
4443 2081 47
StuI AGG'CCT
Cuts at: 1517 2213 1517
Size: 696 5875
StyI C'CwwG_G
Cuts at: 513 1065 2004 2730 3131 4010 513
Size: 552 939 726 401 879 3074
Fragments arranged by size:
3074 939 879 726 552 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4598 4615 4768 4953 5398 325
Size: 1255 3018 17 153 185 445 1498
Fragments arranged by size:
3018 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 4817 6529
Size: 274 80 33 51 1443 285 2555 1712
Cuts at: 6529 96
Size: 138
Fragments arranged by size:
2555 1712 1443 285 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2165 2180 2197 2212 2428 2460 2682 2714 2804
Size: 15 17 15 216 32 222 32 90
Cuts at: 2804 2836 3158 3190 3807 3839 6531 6563 2165
Size: 32 322 32 617 32 2692 32 2173
Fragments arranged by size:
2692 2173 617 322 222 216 90 32
32 32 32 32 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6571
XbaI T'CTAG_A
Cuts at: 3907 3907
Size: 6571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2143 2143
Size: 6571
XhoI C'TCGA_G
Cuts at: 1943 2292 1943
Size: 349 6222
XmaI C'CCGG_G
Cuts at: 1870 3896 1870
Size: 2026 4545
XmnI GAAnn'nnTTC
Cuts at: 4700 4700
Size: 6571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4379 276
Size: 53 83 186 936 2845 2468
Fragments arranged by size:
2845 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BstXI BstZ17I Bsu36I FseI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI
SrfI SwaI