Restriction map for
pFN21AE2026
(Circular) (Six-base) MAPSORT of: pFN21AE2026.seq Check: 7778 from: 1 to: 6532
pFN21AE2026 6532 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2209 2389 2209
Size: 180 6352
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3324 4342 278
Size: 53 83 186 936 1788 1018 2468
Fragments arranged by size:
2468 1788 1018 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3875 1937
Size: 1938 4594
Acc65I G'GTAC_C
Cuts at: 3853 3853
Size: 6532
AclI AA'CG_TT
Cuts at: 1563 4659 5032 1563
Size: 3096 373 3063
Fragments arranged by size:
3096 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2333 3712 4595 5979 6247 1632
Size: 701 1379 883 1384 268 1917
Fragments arranged by size:
1917 1384 1379 883 701 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6335
AflIII A'CryG_T
Cuts at: 1203 5431 6408 1203
Size: 4228 977 1327
Fragments arranged by size:
4228 1327 977
AgeI A'CCGG_T
Cuts at: 5363 5363
Size: 6532
AhdI GACnn_n'nnGTC
Cuts at: 5261 5261
Size: 6532
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3539 1184
Size: 2355 4177
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 6500
AlwNI CAG_nnn'CTG
Cuts at: 2247 4275 5847 2247
Size: 2028 1572 2932
Fragments arranged by size:
2932 2028 1572
ApaI G_GGCC'C
Cuts at: 2668 3661 2668
Size: 993 5539
ApaLI G'TGCA_C
Cuts at: 2921 4589 5745 2921
Size: 1668 1156 3708
Fragments arranged by size:
3708 1668 1156
ApoI r'AATT_y
Cuts at: 1493 3353 3841 4067 4219 6328 1493
Size: 1860 488 226 152 2109 1697
Fragments arranged by size:
2109 1860 1697 488 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5086 160
Size: 872 4054 1606
Fragments arranged by size:
4054 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
AvaI C'yCGr_G
Cuts at: 1870 1943 3857 1870
Size: 73 1914 4545
Fragments arranged by size:
4545 1914 73
BamHI G'GATC_C
Cuts at: 3862 4229 3862
Size: 367 6165
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2012 3853 5308 618
Size: 325 217 627 225 1841 1455 1842
Fragments arranged by size:
1842 1841 1455 627 325 225 217
BanII G_rGCy'C
Cuts at: 1391 2469 2668 3661 3851 1391
Size: 1078 199 993 190 4072
Fragments arranged by size:
4072 1078 993 199 190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5561
BbvCI CC'TCA_GC
Cuts at: 2639 3386 2639
Size: 747 5785
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2341 2375 3475 3509 4723 4757 1903
Size: 34 404 34 1100 34 1214 34 3678
Fragments arranged by size:
3678 1214 1100 404 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4424 5634 4424
Size: 1210 5322
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6532
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2965 5143 136
Size: 107 122 71 631 605 1293 2178 1525
Fragments arranged by size:
2178 1525 1293 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6527 6527
Size: 6532
BlpI GC'TnA_GC
Cuts at: 2295 3086 3949 2295
Size: 791 863 4878
Fragments arranged by size:
4878 863 791
Bme1580I G_kGCm'C
Cuts at: 1790 2668 2925 3661 4593 5749 1790
Size: 878 257 736 932 1156 2573
Fragments arranged by size:
2573 1156 932 878 736 257
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6532
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4263 5221 442
Size: 416 53 83 399 24 2846 958 1753
Fragments arranged by size:
2846 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6532
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 3181 3253 3535 2524
Size: 657 72 282 5521
Fragments arranged by size:
5521 657 282 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2483 2603 3047 3245 3448 3695 3748 3756
Size: 1244 120 444 198 203 247 53 8
Cuts at: 3756 5192 1239
Size: 1436 2579
Fragments arranged by size:
2579 1436 1244 444 247 203 198 120
53 8
Bpu10I CC'TnA_GC
Cuts at: 2639 2753 3386 2639
Size: 114 633 5785
Fragments arranged by size:
5785 633 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2762 2912 4657 5522 5820 2762
Size: 150 1745 865 298 3474
Fragments arranged by size:
3474 1745 865 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2777 2801 2834 5195 882
Size: 617 1278 24 33 2361 2219
Fragments arranged by size:
2361 2219 1278 617 33 24
BsaAI yAC'GTr
Cuts at: 493 1204 2692 4323 493
Size: 711 1488 1631 2702
Fragments arranged by size:
2702 1631 1488 711
BsaBI GATnn'nnATC
Cuts at: 3114 6526 3114
Size: 3412 3120
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2875 3321 4339 4721
Size: 53 83 186 936 1342 446 1018 382
Cuts at: 4721 5356 275
Size: 635 1451
Fragments arranged by size:
1451 1342 1018 936 635 446 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3309 4965 5363 5637 5784 6195 3309
Size: 1656 398 274 147 411 3646
Fragments arranged by size:
3646 1656 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2132 2162 2237 2267 3290
Size: 30 165 30 729 30 75 30 1023
Cuts at: 3290 3320 1178
Size: 30 4390
Fragments arranged by size:
4390 1023 729 165 75 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2140 3044 3242 3452 3800 3803 1186
Size: 874 80 904 198 210 348 3 3915
Fragments arranged by size:
3915 904 874 348 210 198 80 3
BseYI C'CCAG_C
Cuts at: 2263 2974 5735 6231 2263
Size: 711 2761 496 2564
Fragments arranged by size:
2761 2564 711 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2020 2545 2645 3669 1163
Size: 573 284 525 100 1024 4026
Fragments arranged by size:
4026 1024 573 525 284 100
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3982 4743 5771 1540
Size: 275 186 1981 761 1028 2301
Fragments arranged by size:
2301 1981 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2925 3282 4593 4678 5749 2925
Size: 357 1311 85 1071 3708
Fragments arranged by size:
3708 1311 1071 357 85
BsmI GAATG_Cn'
Cuts at: 4043 4136 4043
Size: 93 6439
BsmBI CGTCTCn'nnnn_
Cuts at: 2617 2617
Size: 6532
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2469 2668 2925 3282 3661 3851 4593
Size: 399 679 199 257 357 379 190 742
Cuts at: 4593 4678 5749 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 679 399 379 357 257
199 190 85
BspEI T'CCGG_A
Cuts at: 3309 6195 3309
Size: 2886 3646
BspHI T'CATG_A
Cuts at: 2124 2631 4421 6151 2124
Size: 507 1790 1730 2505
Fragments arranged by size:
2505 1790 1730 507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2209 2389 3090 3887 844
Size: 1365 180 701 797 3489
Fragments arranged by size:
3489 1365 797 701 180
BsrBI CCG'CTC
Cuts at: 1394 1823 2587 4419 1394
Size: 429 764 1832 3507
Fragments arranged by size:
3507 1832 764 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5027 5201 65
Size: 1183 3779 174 1396
Fragments arranged by size:
3779 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2871 5176 5363 1700
Size: 110 1061 2305 187 2869
Fragments arranged by size:
2869 2305 1061 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6532
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6532
BssSI C'ACGA_G
Cuts at: 3190 3269 4592 5604 3190
Size: 79 1323 1012 4118
Fragments arranged by size:
4118 1323 1012 79
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2110 794
Size: 1316 5216
BstBI TT'CG_AA
Cuts at: 5336 5336
Size: 6532
BstEII G'GTnAC_C
Cuts at: 1184 3043 1184
Size: 1859 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 2476
Size: 6532
BstYI r'GATC_y
Cuts at: 1972 2250 2520 3777 3862 4229 4614 4631 6083
Size: 278 270 1257 85 367 385 17 1452
Cuts at: 6083 6169 6181 6527 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 278 270
86 85 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2056 2469 3039 3367 513
Size: 552 939 52 413 570 328 3678
Fragments arranged by size:
3678 939 570 552 413 328 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5793
Fragments arranged by size:
5793 683 56
BtsI GCAGTG_nn'
Cuts at: 2745 2954 3544 3626 4048 4842 4862 2745
Size: 209 590 82 422 794 20 4415
Fragments arranged by size:
4415 794 590 422 209 82 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6532
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6497
DraI TTT'AAA
Cuts at: 3836 3836
Size: 6532
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2576 5539 809
Size: 507 1260 2963 1802
Fragments arranged by size:
2963 1802 1260 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 3036 3056
Size: 54 1425 50 275 18 333 873 20
Cuts at: 3056 3334 3979 4868 6497 8
Size: 278 645 889 1629 43
Fragments arranged by size:
1629 1425 889 873 645 333 278 275
54 50 43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3979 1537
Size: 275 2167 4090
Fragments arranged by size:
4090 2167 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 4462 1353
Size: 1253 1856 3423
Fragments arranged by size:
3423 1856 1253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2245 2495 2711 5119 5493 5639
Size: 1064 138 656 250 216 2408 374 146
Cuts at: 5639 387
Size: 1280
Fragments arranged by size:
2408 1280 1064 656 374 250 216 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2483 2603 3047 3245 3448 3695
Size: 393 701 150 120 444 198 203 247
Cuts at: 3695 3712 3748 3756 4595 5192 5979 6247 1239
Size: 17 36 8 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 701 597 444 393 268
247 203 198 150 120 36 17 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 2528 2027
Size: 501 6031
EcoO109I rG'GnC_Cy
Cuts at: 2098 2665 2749 2932 3265 3400 3580 3657 2098
Size: 567 84 183 333 135 180 77 4973
Fragments arranged by size:
4973 567 333 183 180 135 84 77
EcoRI G'AATT_C
Cuts at: 3353 3841 4219 3353
Size: 488 378 5666
Fragments arranged by size:
5666 488 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 6532
FspI TGC'GCA
Cuts at: 2114 5038 2114
Size: 2924 3608
FspAI rTGC'GCAy
Cuts at: 2114 2114
Size: 6532
HaeII r_GCGC'y
Cuts at: 1678 3393 5679 1678
Size: 1715 2286 2531
Fragments arranged by size:
2531 2286 1715
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3620 3652 5173 5205 5247
Size: 32 135 32 2048 32 1521 32 42
Cuts at: 5247 5279 1373
Size: 32 2626
Fragments arranged by size:
2626 2048 1521 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3876 4122 669
Size: 988 281 1938 246 3079
Fragments arranged by size:
3079 1938 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6532
HpaI GTT'AAC
Cuts at: 4122 4122
Size: 6532
KpnI G_GTAC'C
Cuts at: 3857 3857
Size: 6532
MfeI C'AATT_G
Cuts at: 4131 4131
Size: 6532
MluI A'CGCG_T
Cuts at: 6408 6408
Size: 6532
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5646 5830 1305
Size: 4341 184 2007
Fragments arranged by size:
4341 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3038 3336 10
Size: 54 1425 343 1206 298 3206
Fragments arranged by size:
3206 1425 1206 343 298 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2732 4490 4849 5008 518
Size: 648 1566 1758 359 159 2042
Fragments arranged by size:
2042 1758 1566 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2107 2158 2267 2410 2662 2713 2950
Size: 452 49 51 109 143 252 51 237
Cuts at: 2950 3142 3154 3948 4625 5773 6018 1606
Size: 192 12 794 677 1148 245 2120
Fragments arranged by size:
2120 1148 794 677 452 252 245 237
192 143 109 51 51 49 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 2469 3039 513
Size: 552 939 465 570 4006
Fragments arranged by size:
4006 939 570 552 465
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6532
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6532
NotI GC'GGCC_GC
Cuts at: 3979 3979
Size: 6532
NspI r_CATG'y
Cuts at: 1132 1229 2119 3890 5435 1132
Size: 97 890 1771 1545 2229
Fragments arranged by size:
2229 1771 1545 890 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6532
PciI A'CATG_T
Cuts at: 5431 5431
Size: 6532
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6532
PfoI T'CCnGG_A
Cuts at: 2084 2192 2580 3024 3507 2084
Size: 108 388 444 483 5109
Fragments arranged by size:
5109 483 444 388 108
PmeI GTTT'AAAC
Cuts at: 3836 3836
Size: 6532
PmlI CAC'GTG
Cuts at: 2692 2692
Size: 6532
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 4601 4633 6114 6146 3290
Size: 32 1279 32 1481 32 3676
Fragments arranged by size:
3676 1481 1279 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2098 2932 3400 2098
Size: 834 468 5230
Fragments arranged by size:
5230 834 468
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6532
PsiI TTA'TAA
Cuts at: 4102 4102
Size: 6532
PspOMI G'GGCC_C
Cuts at: 2664 3657 2664
Size: 993 5539
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 6500
PstI C_TGCA'G
Cuts at: 830 2220 2718 2949 3153 3884 830
Size: 1390 498 231 204 731 3478
Fragments arranged by size:
3478 1390 731 498 231 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6532
PvuII CAG'CTG
Cuts at: 2107 2158 2713 2950 3142 2107
Size: 51 555 237 192 5497
Fragments arranged by size:
5497 555 237 192 51
SacII CC_GC'GG
Cuts at: 2059 2059
Size: 6532
SalI G'TCGA_C
Cuts at: 1936 3874 1936
Size: 1938 4594
SanDI GG'GwC_CC
Cuts at: 2098 2098
Size: 6532
SbfI CC_TGCA'GG
Cuts at: 2220 3884 2220
Size: 1664 4868
ScaI AGT'ACT
Cuts at: 4780 6492 4780
Size: 1712 4820
SexAI A'CCwGG_T
Cuts at: 2235 2934 2235
Size: 699 5833
SfcI C'TryA_G
Cuts at: 826 1045 2216 2708 2714 2945 3149 3880 5015
Size: 219 1171 492 6 231 204 731 1135
Cuts at: 5015 5696 5887 826
Size: 681 191 1471
Fragments arranged by size:
1471 1171 1135 731 681 492 231 219
204 191 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6532
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6532
SmaI CCC'GGG
Cuts at: 1872 3859 1872
Size: 1987 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2777 2927 4636 5537 5799 820
Size: 197 926 834 150 1709 901 262 1553
Fragments arranged by size:
1709 1553 926 901 834 262 197 150
SnaBI TAC'GTA
Cuts at: 493 4323 493
Size: 3830 2702
SpeI A'CTAG_T
Cuts at: 152 2839 152
Size: 2687 3845
SphI G_CATG'C
Cuts at: 1132 3890 1132
Size: 2758 3774
SspI AAT'ATT
Cuts at: 5 52 4456 5
Size: 47 4404 2081
Fragments arranged by size:
4404 2081 47
StuI AGG'CCT
Cuts at: 1517 3769 1517
Size: 2252 4280
StyI C'CwwG_G
Cuts at: 513 1065 2004 2469 3039 3971 513
Size: 552 939 465 570 932 3074
Fragments arranged by size:
3074 939 932 570 552 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2611 2906 3715 4559 4576 4729 4914
Size: 1255 1031 295 809 844 17 153 185
Cuts at: 4914 5359 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1031 844 809 445 295 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3064 4778 6490
Size: 274 80 33 51 1443 1087 1714 1712
Cuts at: 6490 96
Size: 138
Fragments arranged by size:
1714 1712 1443 1087 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2670 2702 6492 6524 2670
Size: 32 3790 32 2678
Fragments arranged by size:
3790 2678 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2372 1316
Size: 1056 5476
XbaI T'CTAG_A
Cuts at: 3868 3868
Size: 6532
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3253 3365 3416 3472 3253
Size: 112 51 56 6313
Fragments arranged by size:
6313 112 56 51
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6532
XmaI C'CCGG_G
Cuts at: 1870 3857 1870
Size: 1987 4545
XmnI GAAnn'nnTTC
Cuts at: 4661 4661
Size: 6532
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3322 4340 276
Size: 53 83 186 936 1788 1018 2468
Fragments arranged by size:
2468 1788 1018 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI RsrII SacI SapI
SfiI SfoI SrfI SwaI