Restriction map for
pFN21AE2023
(Circular) (Six-base) MAPSORT of: pFN21AE2023.seq Check: 8715 from: 1 to: 6502
pFN21AE2023 6502 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3674 3845 1937
Size: 1737 171 4594
Fragments arranged by size:
4594 1737 171
Acc65I G'GTAC_C
Cuts at: 3823 3823
Size: 6502
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3475 3572 4565 5949 6217 1632
Size: 1843 97 993 1384 268 1917
Fragments arranged by size:
1917 1843 1384 993 268 97
AfeI AGC'GCT
Cuts at: 2777 2996 2777
Size: 219 6283
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 2647 5401 6378 1203
Size: 1444 2754 977 1327
Fragments arranged by size:
2754 1444 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 2417 5231 2417
Size: 2814 3688
AleI CACnn'nnGTG
Cuts at: 1166 3492 3795 1166
Size: 2326 303 3873
Fragments arranged by size:
3873 2326 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3777 1184
Size: 2593 3909
AlwNI CAG_nnn'CTG
Cuts at: 2218 3277 3617 3742 3757 4245 5817 2218
Size: 1059 340 125 15 488 1572 2903
Fragments arranged by size:
2903 1572 1059 488 340 125 15
ApaI G_GGCC'C
Cuts at: 2862 3154 3255 3392 2862
Size: 292 101 137 5972
Fragments arranged by size:
5972 292 137 101
ApaLI G'TGCA_C
Cuts at: 4559 5715 4559
Size: 1156 5346
ApoI r'AATT_y
Cuts at: 1493 2066 3811 4037 4189 6298 1493
Size: 573 1745 226 152 2109 1697
Fragments arranged by size:
2109 1745 1697 573 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 3316 3396 3537 3827 1870
Size: 73 1373 80 141 290 4545
Fragments arranged by size:
4545 1373 290 141 80 73
BamHI G'GATC_C
Cuts at: 3832 4199 3832
Size: 367 6135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2345 3823 5278 618
Size: 325 217 627 306 252 1478 1455 1842
Fragments arranged by size:
1842 1478 1455 627 325 306 252 217
BanII G_rGCy'C
Cuts at: 1391 2862 3015 3049 3154 3255 3392 3403 3438
Size: 1471 153 34 105 101 137 11 35
Cuts at: 3438 3597 3611 3629 3821 1391
Size: 159 14 18 192 4072
Fragments arranged by size:
4072 1471 192 159 153 137 105 101
35 34 18 14 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2458 928
Size: 971 559 4972
Fragments arranged by size:
4972 971 559
BbvCI CC'TCA_GC
Cuts at: 2124 2316 2124
Size: 192 6310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3199 3233 4693 4727 1903
Size: 34 1262 34 1460 34 3678
Fragments arranged by size:
3678 1460 1262 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4394 5604 4394
Size: 1210 5292
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2091 3633 5113
Size: 107 122 71 631 605 419 1542 1480
Cuts at: 5113 136
Size: 1525
Fragments arranged by size:
1542 1525 1480 631 605 419 122 107
71
BglII A'GATC_T
Cuts at: 6497 6497
Size: 6502
BlpI GC'TnA_GC
Cuts at: 3919 3919
Size: 6502
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2580 2862 3154 3255 3392 4563 5719
Size: 306 484 282 292 101 137 1171 1156
Cuts at: 5719 1790
Size: 2573
Fragments arranged by size:
2573 1171 1156 484 306 292 282 137
101
BmgBI CAC'GTC
Cuts at: 1318 2575 1318
Size: 1257 5245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2855 3085 3593
Size: 416 53 83 399 24 1438 230 508
Cuts at: 3593 3789 4233 5191 442
Size: 196 444 958 1753
Fragments arranged by size:
1753 1438 958 508 444 416 399 230
196 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6502
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2149 2485 3790 2149
Size: 336 1305 4861
Fragments arranged by size:
4861 1305 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2387 2528 2569 2630 3353 5162 1239
Size: 1148 141 41 61 723 1809 2579
Fragments arranged by size:
2579 1809 1148 723 141 61 41
Bpu10I CC'TnA_GC
Cuts at: 2124 2316 2124
Size: 192 6310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4627 5492 5790 4627
Size: 865 298 5339
Fragments arranged by size:
5339 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2404 5165 882
Size: 617 905 2761 2219
Fragments arranged by size:
2761 2219 905 617
BsaAI yAC'GTr
Cuts at: 493 1204 4293 493
Size: 711 3089 2702
Fragments arranged by size:
3089 2702 711
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2223 4309 4691 5326
Size: 53 83 186 936 690 2086 382 635
Cuts at: 5326 275
Size: 1451
Fragments arranged by size:
2086 1451 936 690 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2169 2302 4935 5333 5607 5754 6165 2169
Size: 133 2633 398 274 147 411 2506
Fragments arranged by size:
2633 2506 411 398 274 147 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6277
Fragments arranged by size:
6277 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 2801 3020 3381 3384 1186
Size: 955 660 219 361 3 4304
Fragments arranged by size:
4304 955 660 361 219 3
BseYI C'CCAG_C
Cuts at: 2176 2218 2248 2861 2991 3145 3439 3750 3786
Size: 42 30 613 130 154 294 311 36
Cuts at: 3786 5705 6201 2176
Size: 1919 496 2477
Fragments arranged by size:
2477 1919 613 496 311 294 154 130
42 36 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3145 3623 1163
Size: 573 1409 478 4042
Fragments arranged by size:
4042 1409 573 478
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3952 4713 5741 1540
Size: 275 186 1951 761 1028 2301
Fragments arranged by size:
2301 1951 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2907 3015 3438 4563 4648 5719 2907
Size: 108 423 1125 85 1071 3690
Fragments arranged by size:
3690 1125 1071 423 108 85
BsiWI C'GTAC_G
Cuts at: 3291 3291
Size: 6502
BsmI GAATG_Cn'
Cuts at: 4013 4106 4013
Size: 93 6409
BsmBI CGTCTCn'nnnn_
Cuts at: 3301 3301
Size: 6502
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2580 2862 2907 3015 3049 3154
Size: 399 306 484 282 45 108 34 105
Cuts at: 3154 3255 3392 3403 3438 3597 3611 3629 3821
Size: 101 137 11 35 159 14 18 192
Cuts at: 3821 4563 4648 5719 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 399 306 282 192
159 137 108 105 101 85 45 35
34 18 14 11
BspEI T'CCGG_A
Cuts at: 2169 6165 2169
Size: 3996 2506
BspHI T'CATG_A
Cuts at: 4391 6121 4391
Size: 1730 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3857 844
Size: 3013 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2284 2512 2761 2791 2977 4389 1394
Size: 429 461 228 249 30 186 1412 3507
Fragments arranged by size:
3507 1412 461 429 249 228 186 30
BsrDI GCAATG_nn'
Cuts at: 65 1248 4997 5171 65
Size: 1183 3749 174 1396
Fragments arranged by size:
3749 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2850 3069 5146 5333 1700
Size: 110 1040 219 2077 187 2869
Fragments arranged by size:
2869 2077 1040 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6502
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 4562 5574 4562
Size: 1012 5490
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2599 794
Size: 1805 4697
BstBI TT'CG_AA
Cuts at: 5306 5306
Size: 6502
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6502
BstYI r'GATC_y
Cuts at: 1972 2196 2622 3428 3768 3832 4199 4584 4601
Size: 224 426 806 340 64 367 385 17
Cuts at: 4601 6053 6139 6151 6497 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 806 426 385 367 346 340
224 86 64 17 12
BstZ17I GTA'TAC
Cuts at: 3675 3675
Size: 6502
Bsu36I CC'TnA_GG
Cuts at: 2352 2352
Size: 6502
BtgI C'CryG_G
Cuts at: 513 1065 2190 2333 2449 3474 3629 513
Size: 552 1125 143 116 1025 155 3386
Fragments arranged by size:
3386 1125 1025 552 155 143 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5763
Fragments arranged by size:
5763 683 56
BtsI GCAGTG_nn'
Cuts at: 2499 3128 3241 3563 4018 4812 4832 2499
Size: 629 113 322 455 794 20 4169
Fragments arranged by size:
4169 794 629 455 322 113 20
ClaI AT'CG_AT
Cuts at: 1554 2424 1554
Size: 870 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2880 2915 529
Size: 35 2316 35 4116
Fragments arranged by size:
4116 2316 35 35
DraI TTT'AAA
Cuts at: 3806 3806
Size: 6502
DraIII CAC_nnn'GTG
Cuts at: 3709 3709
Size: 6502
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2439 2978
Size: 54 1425 50 275 18 288 321 539
Cuts at: 2978 3067 3466 3484 3651 3949 4838 6467 8
Size: 89 399 18 167 298 889 1629 43
Fragments arranged by size:
1629 1425 889 539 399 321 298 288
275 167 89 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3949 1537
Size: 275 2137 4090
Fragments arranged by size:
4090 2137 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 4432 1353
Size: 1026 2053 3423
Fragments arranged by size:
3423 2053 1026
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3013 3436 3013
Size: 423 6079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2528 2569 2630 3353 3475 3572
Size: 393 755 141 41 61 723 122 97
Cuts at: 3572 4565 5162 5949 6217 1239
Size: 993 597 787 268 1524
Fragments arranged by size:
1524 993 787 755 723 597 393 268
141 122 97 61 41
EcoNI CCTnn'n_nnAGG
Cuts at: 2929 3653 3710 2929
Size: 724 57 5721
Fragments arranged by size:
5721 724 57
EcoO109I rG'GnC_Cy
Cuts at: 3150 3168 3374 3150
Size: 18 206 6278
Fragments arranged by size:
6278 206 18
EcoRI G'AATT_C
Cuts at: 3811 4189 3811
Size: 378 6124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2482 2179
Size: 303 6199
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 2293 2779 2998 3730 5649 1678
Size: 615 486 219 732 1919 2531
Fragments arranged by size:
2531 1919 732 615 486 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2403 2435 3420 3452 3533
Size: 32 135 32 831 32 985 32 81
Cuts at: 3533 3565 5143 5175 5217 5249 1373
Size: 32 1578 32 42 32 2626
Fragments arranged by size:
2626 1578 985 831 135 81 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3846 4092 669
Size: 988 281 1908 246 3079
Fragments arranged by size:
3079 1908 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6502
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KpnI G_GTAC'C
Cuts at: 3827 3827
Size: 6502
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 2647 6378 2647
Size: 3731 2771
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2754 3327 5616 5800 1305
Size: 1449 573 2289 184 2007
Fragments arranged by size:
2289 2007 1449 573 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 3486 3653 10
Size: 54 1425 343 609 1045 167 2859
Fragments arranged by size:
2859 1425 1045 609 343 167 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 3492 3795 4460 4819 4978 518
Size: 648 1365 961 303 665 359 159 2042
Fragments arranged by size:
2042 1365 961 665 648 359 303 159
MspA1I CmG'CkG
Cuts at: 1606 2123 2335 3757 3918 4595 5743 5988 1606
Size: 517 212 1422 161 677 1148 245 2120
Fragments arranged by size:
2120 1422 1148 677 517 245 212 161
NaeI GCC'GGC
Cuts at: 2852 3071 2852
Size: 219 6283
NcoI C'CATG_G
Cuts at: 513 1065 2449 3474 513
Size: 552 1384 1025 3541
Fragments arranged by size:
3541 1384 1025 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6502
NgoMIV G'CCGG_C
Cuts at: 2850 3069 2850
Size: 219 6283
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6502
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NspI r_CATG'y
Cuts at: 1132 1229 3860 5405 1132
Size: 97 2631 1545 2229
Fragments arranged by size:
2631 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3164 3611 1348
Size: 1816 447 4239
Fragments arranged by size:
4239 1816 447
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3261 3617 3757 1108
Size: 2153 356 140 3853
Fragments arranged by size:
3853 2153 356 140
PfoI T'CCnGG_A
Cuts at: 2034 2546 3741 2034
Size: 512 1195 4795
Fragments arranged by size:
4795 1195 512
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 6084 6116 4571
Size: 32 1481 32 4957
Fragments arranged by size:
4957 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3168 3168
Size: 6502
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PspOMI G'GGCC_C
Cuts at: 2858 3150 3251 3388 2858
Size: 292 101 137 5972
Fragments arranged by size:
5972 292 137 101
PstI C_TGCA'G
Cuts at: 830 2151 2214 3854 830
Size: 1321 63 1640 3478
Fragments arranged by size:
3478 1640 1321 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 3757 3757
Size: 6502
SacI G_AGCT'C
Cuts at: 3015 3438 3015
Size: 423 6079
SacII CC_GC'GG
Cuts at: 2336 2336
Size: 6502
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SanDI GG'GwC_CC
Cuts at: 3168 3168
Size: 6502
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 3545 4750 6462 3545
Size: 1205 1712 3585
Fragments arranged by size:
3585 1712 1205
SexAI A'CCwGG_T
Cuts at: 3705 3705
Size: 6502
SfcI C'TryA_G
Cuts at: 826 1045 2147 2210 3415 3850 4985 5666 5857
Size: 219 1102 63 1205 435 1135 681 191
Cuts at: 5857 826
Size: 1471
Fragments arranged by size:
1471 1205 1135 1102 681 435 219 191
63
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 3829 1872
Size: 1957 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3537 4606 5507 5769 820
Size: 197 926 1594 1069 901 262 1553
Fragments arranged by size:
1594 1553 1069 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 2132 1517
Size: 615 5887
StyI C'CwwG_G
Cuts at: 513 1065 2099 2449 2752 3474 3654 3941 513
Size: 552 1034 350 303 722 180 287 3074
Fragments arranged by size:
3074 1034 722 552 350 303 287 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2240 2642 2985 3467 3589 4529 4546
Size: 1255 660 402 343 482 122 940 17
Cuts at: 4546 4699 4884 5329 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 940 660 482 445 402 343
185 153 122 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2802 3543 4748
Size: 274 80 33 51 1443 825 741 1205
Cuts at: 4748 6460 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1205 825 741 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6462 6494 6462
Size: 32 6470
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6502
XbaI T'CTAG_A
Cuts at: 3838 3838
Size: 6502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2245 2245
Size: 6502
XhoI C'TCGA_G
Cuts at: 1943 3537 1943
Size: 1594 4908
XmaI C'CCGG_G
Cuts at: 1870 3827 1870
Size: 1957 4545
XmnI GAAnn'nnTTC
Cuts at: 2777 4631 2777
Size: 1854 4648
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BstXI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SapI SfiI SfoI SrfI
SwaI