Restriction map for
pFN21AE2021
(Circular) (Six-base) MAPSORT of: pFN21AE2021.seq Check: 9466 from: 1 to: 6502
pFN21AE2021 6502 bp
With 182 enzymes: SgfI *
July 6, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2303 3845 1937
Size: 366 1542 4594
Fragments arranged by size:
4594 1542 366
Acc65I G'GTAC_C
Cuts at: 3823 3823
Size: 6502
AclI AA'CG_TT
Cuts at: 1563 3300 4629 5002 1563
Size: 1737 1329 373 3063
Fragments arranged by size:
3063 1737 1329 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2550 2833 4565 5949 6217 1632
Size: 918 283 1732 1384 268 1917
Fragments arranged by size:
1917 1732 1384 918 283 268
AfeI AGC'GCT
Cuts at: 2331 2331
Size: 6502
AflII C'TTAA_G
Cuts at: 820 1017 3137 820
Size: 197 2120 4185
Fragments arranged by size:
4185 2120 197
AflIII A'CryG_T
Cuts at: 1203 5401 6378 1203
Size: 4198 977 1327
Fragments arranged by size:
4198 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 3634 5231 3634
Size: 1597 4905
AleI CACnn'nnGTG
Cuts at: 1166 2748 1166
Size: 1582 4920
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 6470
AlwNI CAG_nnn'CTG
Cuts at: 4245 5817 4245
Size: 1572 4930
ApaI G_GGCC'C
Cuts at: 3079 3079
Size: 6502
ApaLI G'TGCA_C
Cuts at: 2741 4559 5715 2741
Size: 1818 1156 3528
Fragments arranged by size:
3528 1818 1156
ApoI r'AATT_y
Cuts at: 1493 2368 3811 4037 4189 6298 1493
Size: 875 1443 226 152 2109 1697
Fragments arranged by size:
2109 1697 1443 875 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 3072 3827 1870
Size: 73 1129 755 4545
Fragments arranged by size:
4545 1129 755 73
AvrII C'CTAG_G
Cuts at: 3547 3547
Size: 6502
BamHI G'GATC_C
Cuts at: 2046 2487 2905 3543 3832 4199 2046
Size: 441 418 638 289 367 4349
Fragments arranged by size:
4349 638 441 418 367 289
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3823 5278 618
Size: 325 217 627 2036 1455 1842
Fragments arranged by size:
2036 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3079 3821 1391
Size: 1688 742 4072
Fragments arranged by size:
4072 1688 742
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2028 2390 3369 928
Size: 971 129 362 979 4061
Fragments arranged by size:
4061 979 971 362 129
BbvCI CC'TCA_GC
Cuts at: 2171 2591 3500 2171
Size: 420 909 5173
Fragments arranged by size:
5173 909 420
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4693 4727 1903
Size: 34 2756 34 3678
Fragments arranged by size:
3678 2756 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4394 5604 4394
Size: 1210 5292
BclI T'GATC_A
Cuts at: 1548 3010 1548
Size: 1462 5040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3770 5113 136
Size: 107 122 71 631 605 2098 1343 1525
Fragments arranged by size:
2098 1525 1343 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2839 6497 2839
Size: 3658 2844
BlpI GC'TnA_GC
Cuts at: 3919 3919
Size: 6502
Bme1580I G_kGCm'C
Cuts at: 1790 2603 2745 3079 4563 5719 1790
Size: 813 142 334 1484 1156 2573
Fragments arranged by size:
2573 1484 1156 813 334 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3362 3760 4233
Size: 416 53 83 399 24 1945 398 473
Cuts at: 4233 5191 442
Size: 958 1753
Fragments arranged by size:
1945 1753 958 473 416 399 398 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6502
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 6502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2877 3761 5162 1239
Size: 1638 884 1401 2579
Fragments arranged by size:
2579 1638 1401 884
Bpu10I CC'TnA_GC
Cuts at: 2171 2591 3500 2171
Size: 420 909 5173
Fragments arranged by size:
5173 909 420
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2743 2864 4627 5492 5790 2743
Size: 121 1763 865 298 3455
Fragments arranged by size:
3455 1763 865 298 121
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2347 5165 882
Size: 617 848 2818 2219
Fragments arranged by size:
2818 2219 848 617
BsaAI yAC'GTr
Cuts at: 493 1204 2125 4293 493
Size: 711 921 2168 2702
Fragments arranged by size:
2702 2168 921 711
BsaBI GATnn'nnATC
Cuts at: 2910 6496 2910
Size: 3586 2916
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 4309 4691 5326
Size: 53 83 186 936 486 2290 382 635
Cuts at: 5326 275
Size: 1451
Fragments arranged by size:
2290 1451 936 635 486 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4935 5333 5607 5754 6165 4935
Size: 398 274 147 411 5272
Fragments arranged by size:
5272 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3245 3275 3773 3803 1178
Size: 30 165 30 1842 30 498 30 3877
Fragments arranged by size:
3877 1842 498 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 3683 3710 1186
Size: 973 1524 27 3978
Fragments arranged by size:
3978 1524 973 27
BseYI C'CCAG_C
Cuts at: 5705 6201 5705
Size: 496 6006
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2135 1163
Size: 573 399 5530
Fragments arranged by size:
5530 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2142 3952 4713 5741 1540
Size: 275 186 141 1810 761 1028 2301
Fragments arranged by size:
2301 1810 1028 761 275 186 141
BsiHKAI G_wGCw'C
Cuts at: 2745 4563 4648 5719 2745
Size: 1818 85 1071 3528
Fragments arranged by size:
3528 1818 1071 85
BsmI GAATG_Cn'
Cuts at: 2293 4013 4106 2293
Size: 1720 93 4689
Fragments arranged by size:
4689 1720 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2603 2745 3079 3821 4563 4648 5719
Size: 399 813 142 334 742 742 85 1071
Cuts at: 5719 1391
Size: 2174
Fragments arranged by size:
2174 1071 813 742 742 399 334 142
85
BspEI T'CCGG_A
Cuts at: 6165 6165
Size: 6502
BspHI T'CATG_A
Cuts at: 2269 4391 6121 2269
Size: 2122 1730 2650
Fragments arranged by size:
2650 2122 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3790 3857 844
Size: 2946 67 3489
Fragments arranged by size:
3489 2946 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4389 1394
Size: 429 2566 3507
Fragments arranged by size:
3507 2566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2469 3288 3594 4997 5171 65
Size: 1183 1221 819 306 1403 174 1396
Fragments arranged by size:
1403 1396 1221 1183 819 306 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 5146 5333 1700
Size: 110 245 3091 187 2869
Fragments arranged by size:
3091 2869 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 2538 96
Size: 2442 4060
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 2952 4562 5574 2952
Size: 1610 1012 3880
Fragments arranged by size:
3880 1610 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6502
BstBI TT'CG_AA
Cuts at: 5306 5306
Size: 6502
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6502
BstYI r'GATC_y
Cuts at: 1972 2046 2487 2839 2905 3543 3832 4199 4584
Size: 74 441 352 66 638 289 367 385
Cuts at: 4584 4601 6053 6139 6151 6497 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 638 441 385 367 352 346
289 86 74 66 17 12
BstZ17I GTA'TAC
Cuts at: 2304 2304
Size: 6502
Bsu36I CC'TnA_GG
Cuts at: 2042 2042
Size: 6502
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 2142 2307 3026 3678 513
Size: 552 939 84 54 165 719 652 3337
Fragments arranged by size:
3337 939 719 652 552 165 84 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5763
Fragments arranged by size:
5763 683 56
BtsI GCAGTG_nn'
Cuts at: 2603 3791 4018 4812 4832 2603
Size: 1188 227 794 20 4273
Fragments arranged by size:
4273 1188 794 227 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6467
DraI TTT'AAA
Cuts at: 3806 3806
Size: 6502
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 3949 4838
Size: 54 1425 50 275 18 309 1810 889
Cuts at: 4838 6467 8
Size: 1629 43
Fragments arranged by size:
1810 1629 1425 889 309 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2139 3949 1537
Size: 275 327 1810 4090
Fragments arranged by size:
4090 1810 327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4432 1353
Size: 3079 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2550 2833 2877 3761 4565 5162 5949
Size: 393 918 283 44 884 804 597 787
Cuts at: 5949 6217 1239
Size: 268 1524
Fragments arranged by size:
1524 918 884 804 787 597 393 283
268 44
EcoO109I rG'GnC_Cy
Cuts at: 3076 3736 3076
Size: 660 5842
EcoRI G'AATT_C
Cuts at: 3811 4189 3811
Size: 378 6124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2358 2358
Size: 6502
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 2333 2563 5649 1678
Size: 655 230 3086 2531
Fragments arranged by size:
3086 2531 655 230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2648 2680 2696 2728 2780
Size: 32 135 32 1076 32 16 32 52
Cuts at: 2780 2812 2890 2922 3620 3652 5143 5175 5217
Size: 32 78 32 698 32 1491 32 42
Cuts at: 5217 5249 1373
Size: 32 2626
Fragments arranged by size:
2626 1491 1076 698 135 78 52 42
32 32 32 32 32 32 32 32
32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3846 4092 669
Size: 988 281 1908 246 3079
Fragments arranged by size:
3079 1908 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2830 748
Size: 2082 4420
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KpnI G_GTAC'C
Cuts at: 3827 3827
Size: 6502
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 2883 3288 5616 5800 1305
Size: 876 702 405 2328 184 2007
Fragments arranged by size:
2328 2007 876 702 405 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4680
Fragments arranged by size:
4680 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2672 2748 4460 4819 4978 518
Size: 648 1506 76 1712 359 159 2042
Fragments arranged by size:
2042 1712 1506 648 359 159 76
MspA1I CmG'CkG
Cuts at: 1606 2083 2090 2144 3106 3918 4595 5743 5988
Size: 477 7 54 962 812 677 1148 245
Cuts at: 5988 1606
Size: 2120
Fragments arranged by size:
2120 1148 962 812 677 477 245 54
7
NcoI C'CATG_G
Cuts at: 513 1065 2004 2307 513
Size: 552 939 303 4708
Fragments arranged by size:
4708 939 552 303
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6502
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6502
NotI GC'GGCC_GC
Cuts at: 2139 3949 2139
Size: 1810 4692
NspI r_CATG'y
Cuts at: 1132 1229 3020 3799 3860 5405 1132
Size: 97 1791 779 61 1545 2229
Fragments arranged by size:
2229 1791 1545 779 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6502
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2242 1108
Size: 1134 5368
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PmlI CAC'GTG
Cuts at: 2125 2125
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3773 3805 4571 4603 6084 6116 3773
Size: 32 766 32 1481 32 4159
Fragments arranged by size:
4159 1481 766 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3736 3736
Size: 6502
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PspOMI G'GGCC_C
Cuts at: 3075 3075
Size: 6502
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2764 2796 2764
Size: 32 6470
PstI C_TGCA'G
Cuts at: 830 3854 830
Size: 3024 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 3106 3106
Size: 6502
SacII CC_GC'GG
Cuts at: 2091 2145 2091
Size: 54 6448
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 4750 6462 4750
Size: 1712 4790
SfcI C'TryA_G
Cuts at: 826 1045 3055 3850 4985 5666 5857 826
Size: 219 2010 795 1135 681 191 1471
Fragments arranged by size:
2010 1471 1135 795 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 3074 3829 1872
Size: 1202 755 4545
Fragments arranged by size:
4545 1202 755
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2758 2879 3137 4606 5507 5769
Size: 197 926 815 121 258 1469 901 262
Cuts at: 5769 820
Size: 1553
Fragments arranged by size:
1553 1469 926 901 815 262 258 197
121
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3020 3799 3860 1132
Size: 1888 779 61 3774
Fragments arranged by size:
3774 1888 779 61
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6502
StyI C'CwwG_G
Cuts at: 513 1065 2004 2307 2983 3093 3547 3941 513
Size: 552 939 303 676 110 454 394 3074
Fragments arranged by size:
3074 939 676 552 454 394 303 110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3019 4529 4546 4699 4884 5329 325
Size: 1255 1439 1510 17 153 185 445 1498
Fragments arranged by size:
1510 1498 1439 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2538 2844 4748
Size: 274 80 33 51 1443 561 306 1904
Cuts at: 4748 6460 96
Size: 1712 138
Fragments arranged by size:
1904 1712 1443 561 306 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6462 6494 6462
Size: 32 6470
Tth111I GACn'n_nGTC
Cuts at: 1316 3530 1316
Size: 2214 4288
XbaI T'CTAG_A
Cuts at: 3640 3838 3640
Size: 198 6304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6502
XmaI C'CCGG_G
Cuts at: 1870 3072 3827 1870
Size: 1202 755 4545
Fragments arranged by size:
4545 1202 755
XmnI GAAnn'nnTTC
Cuts at: 4631 4631
Size: 6502
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI
DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspXI RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI