Restriction map for
pFN21AE2016
(Circular) (Six-base) MAPSORT of: pFN21AE2016.seq Check: 5483 from: 1 to: 6484
pFN21AE2016 6484 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2220 2220
Size: 6484
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2322 2340 4294 278
Size: 53 83 186 936 786 18 1954 2468
Fragments arranged by size:
2468 1954 936 786 186 83 53 18
AccI GT'mk_AC
Cuts at: 1937 2046 3827 1937
Size: 109 1781 4594
Fragments arranged by size:
4594 1781 109
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3065 3245 3422 4547 5931 6199 1632
Size: 1433 180 177 1125 1384 268 1917
Fragments arranged by size:
1917 1433 1384 1125 268 180 177
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 2830 3158 5383 6360 1203
Size: 1627 328 2225 977 1327
Fragments arranged by size:
2225 1627 1327 977 328
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2322 1184
Size: 1138 5346
AlwNI CAG_nnn'CTG
Cuts at: 2452 3043 3223 3535 3572 4227 5799 2452
Size: 591 180 312 37 655 1572 3137
Fragments arranged by size:
3137 1572 655 591 312 180 37
ApaI G_GGCC'C
Cuts at: 2240 2916 3742 2240
Size: 676 826 4982
Fragments arranged by size:
4982 826 676
ApaLI G'TGCA_C
Cuts at: 4541 5697 4541
Size: 1156 5328
ApoI r'AATT_y
Cuts at: 1493 2849 3793 4019 4171 6280 1493
Size: 1356 944 226 152 2109 1697
Fragments arranged by size:
2109 1697 1356 944 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5038 160
Size: 872 4006 1606
Fragments arranged by size:
4006 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 3447 3809 1870
Size: 73 91 1413 362 4545
Fragments arranged by size:
4545 1413 362 91 73
AvrII C'CTAG_G
Cuts at: 3367 3367
Size: 6484
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2312 3805 5260 618
Size: 325 217 627 252 273 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 273 252 217
BanII G_rGCy'C
Cuts at: 1391 2240 2586 2916 3153 3209 3742 3803 1391
Size: 849 346 330 237 56 533 61 4072
Fragments arranged by size:
4072 849 533 346 330 237 61 56
BbeI G_GCGC'C
Cuts at: 2043 2316 2043
Size: 273 6211
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5513
BbvCI CC'TCA_GC
Cuts at: 3474 3474
Size: 6484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2235 5095 136
Size: 107 122 71 631 605 563 2860 1525
Fragments arranged by size:
2860 1525 631 605 563 122 107 71
BglII A'GATC_T
Cuts at: 2779 6479 2779
Size: 3700 2784
BlpI GC'TnA_GC
Cuts at: 2244 3216 3686 3901 2244
Size: 972 470 215 4827
Fragments arranged by size:
4827 972 470 215
Bme1580I G_kGCm'C
Cuts at: 1790 2240 2577 2916 3742 4545 5701 1790
Size: 450 337 339 826 803 1156 2573
Fragments arranged by size:
2573 1156 826 803 450 339 337
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6484
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2245 4215 5173
Size: 416 53 83 399 24 828 1970 958
Cuts at: 5173 442
Size: 1753
Fragments arranged by size:
1970 1753 958 828 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2957 3032 3076 5144 1239
Size: 1013 705 75 44 2068 2579
Fragments arranged by size:
2579 2068 1013 705 75 44
Bpu10I CC'TnA_GC
Cuts at: 2805 3474 2805
Size: 669 5815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3020 4609 5474 5772 3020
Size: 1589 865 298 3732
Fragments arranged by size:
3732 1589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2117 2643 2954 5147 882
Size: 617 618 526 311 2193 2219
Fragments arranged by size:
2219 2193 618 617 526 311
BsaAI yAC'GTr
Cuts at: 493 1204 3679 4275 493
Size: 711 2475 596 2702
Fragments arranged by size:
2702 2475 711 596
BsaBI GATnn'nnATC
Cuts at: 2981 6478 2981
Size: 3497 2987
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 2129 2313 2319
Size: 53 83 186 936 507 89 184 6
Cuts at: 2319 2337 4291 4673 5308 275
Size: 18 1954 382 635 1451
Fragments arranged by size:
1954 1451 936 635 507 382 186 184
89 83 53 18 6
BsaWI w'CCGG_w
Cuts at: 2103 4917 5315 5589 5736 6147 2103
Size: 2814 398 274 147 411 2440
Fragments arranged by size:
2814 2440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3135 3165 1178
Size: 30 165 30 1732 30 4497
Fragments arranged by size:
4497 1732 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2972 3079 3467 1186
Size: 1786 107 388 4203
Fragments arranged by size:
4203 1786 388 107
BseYI C'CCAG_C
Cuts at: 5687 6183 5687
Size: 496 5988
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2684 1163
Size: 573 549 399 4963
Fragments arranged by size:
4963 573 549 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2108 3934 4695 5723 1540
Size: 275 186 107 1826 761 1028 2301
Fragments arranged by size:
2301 1826 1028 761 275 186 107
BsiHKAI G_wGCw'C
Cuts at: 2029 2375 3153 3209 3687 4545 4630 5701 2029
Size: 346 778 56 478 858 85 1071 2812
Fragments arranged by size:
2812 1071 858 778 478 346 85 56
BsmI GAATG_Cn'
Cuts at: 3756 3995 4088 3756
Size: 239 93 6152
Fragments arranged by size:
6152 239 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2240 2375 2577 2586 2916 3153
Size: 399 239 211 135 202 9 330 237
Cuts at: 3153 3209 3687 3742 3803 4545 4630 5701 1391
Size: 56 478 55 61 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 478 399 330 239 237
211 202 135 85 61 56 55 9
BspEI T'CCGG_A
Cuts at: 6147 6147
Size: 6484
BspHI T'CATG_A
Cuts at: 4373 6103 4373
Size: 1730 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 3129 3839 844
Size: 1376 909 710 3489
Fragments arranged by size:
3489 1376 909 710
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 4371 1394
Size: 429 1828 720 3507
Fragments arranged by size:
3507 1828 720 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4979 5153 65
Size: 1183 3731 174 1396
Fragments arranged by size:
3731 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2650 3521 3630 5128 5315 1700
Size: 110 840 871 109 1498 187 2869
Fragments arranged by size:
2869 1498 871 840 187 110 109
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6484
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2491 2653 794
Size: 1697 162 4625
Fragments arranged by size:
4625 1697 162
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6484
BstXI CCAn_nnnn'nTGG
Cuts at: 3344 3344
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 2779 3033 3814 4181 4566 4583 6035 6121
Size: 807 254 781 367 385 17 1452 86
Cuts at: 6121 6133 6479 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 807 781 385 367 346 254
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2577 2628 2668 3618 513
Size: 552 1512 51 40 950 3379
Fragments arranged by size:
3379 1512 950 552 51 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5745
Fragments arranged by size:
5745 683 56
BtsI GCAGTG_nn'
Cuts at: 4000 4794 4814 4000
Size: 794 20 5670
Fragments arranged by size:
5670 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 3788 3788
Size: 6484
DraIII CAC_nnn'GTG
Cuts at: 2716 3682 3696 2716
Size: 966 14 5504
Fragments arranged by size:
5504 966 14
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5491 809
Size: 507 4175 1802
Fragments arranged by size:
4175 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2631 2979
Size: 54 1425 50 275 18 264 537 348
Cuts at: 2979 3425 3931 4820 6449 8
Size: 446 506 889 1629 43
Fragments arranged by size:
1629 1425 889 537 506 446 348 275
264 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3931 1537
Size: 275 2119 4090
Fragments arranged by size:
4090 2119 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2783 3066 3197 4414 1353
Size: 1430 283 131 1217 3423
Fragments arranged by size:
3423 1430 1217 283 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2984 3089 5071 5445 5591 387
Size: 1064 138 1395 105 1982 374 146 1280
Fragments arranged by size:
1982 1395 1280 1064 374 146 138 105
EcoICRI GAG'CTC
Cuts at: 3151 3207 3151
Size: 56 6428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2957 3032 3065 3076 3245 3422
Size: 393 620 705 75 33 11 169 177
Cuts at: 3422 4547 5144 5931 6199 1239
Size: 1125 597 787 268 1524
Fragments arranged by size:
1524 1125 787 705 620 597 393 268
177 169 75 33 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2744 2744
Size: 6484
EcoO109I rG'GnC_Cy
Cuts at: 2053 2236 3049 3229 3364 3739 3775 2053
Size: 183 813 180 135 375 36 4762
Fragments arranged by size:
4762 813 375 183 180 135 36
EcoRI G'AATT_C
Cuts at: 2849 3793 4171 2849
Size: 944 378 5162
Fragments arranged by size:
5162 944 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2392 2890 2392
Size: 498 5986
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 2043 2316 2676 5631 1678
Size: 365 273 360 2955 2531
Fragments arranged by size:
2955 2531 365 360 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 3135 3167 5125
Size: 32 135 32 456 32 1075 32 1958
Cuts at: 5125 5157 5199 5231 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1958 1075 456 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2436 3828 4074 669
Size: 988 281 498 1392 246 3079
Fragments arranged by size:
3079 1392 988 498 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6484
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KasI G'GCGC_C
Cuts at: 2039 2312 2039
Size: 273 6211
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 4083 4083
Size: 6484
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2839 5598 5782 1305
Size: 1534 2759 184 2007
Fragments arranged by size:
2759 2007 1534 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2981 3427 10
Size: 54 1425 343 1149 446 3067
Fragments arranged by size:
3067 1425 1149 446 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2304 2844 3342 4442 4801 4960 518
Size: 648 1138 540 498 1100 359 159 2042
Fragments arranged by size:
2042 1138 1100 648 540 498 359 159
MspA1I CmG'CkG
Cuts at: 1606 2134 2508 2517 2918 3040 3400 3506 3620
Size: 528 374 9 401 122 360 106 114
Cuts at: 3620 3900 4577 5725 5970 1606
Size: 280 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 528 401 374 360 280
245 122 114 106 9
NaeI GCC'GGC
Cuts at: 3523 3632 3523
Size: 109 6375
NarI GG'CG_CC
Cuts at: 2040 2313 2040
Size: 273 6211
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5932
NdeI CA'TA_TG
Cuts at: 387 3136 387
Size: 2749 3735
NgoMIV G'CCGG_C
Cuts at: 3521 3630 3521
Size: 109 6375
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NspI r_CATG'y
Cuts at: 1132 1229 3162 3842 5387 1132
Size: 97 1933 680 1545 2229
Fragments arranged by size:
2229 1933 1545 680 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 3158 5383 3158
Size: 2225 4259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2452 3307 1108
Size: 1344 855 4285
Fragments arranged by size:
4285 1344 855
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PmlI CAC'GTG
Cuts at: 3679 3679
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3049 3364 3049
Size: 315 6169
PshAI GACnn'nnGTC
Cuts at: 1613 3557 1613
Size: 1944 4540
PsiI TTA'TAA
Cuts at: 4054 4054
Size: 6484
PspOMI G'GGCC_C
Cuts at: 2236 2912 3738 2236
Size: 676 826 4982
Fragments arranged by size:
4982 826 676
PstI C_TGCA'G
Cuts at: 830 2217 3639 3836 830
Size: 1387 1422 197 3478
Fragments arranged by size:
3478 1422 1387 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2508 2517 3040 3400 2508
Size: 9 523 360 5592
Fragments arranged by size:
5592 523 360 9
SacI G_AGCT'C
Cuts at: 3153 3209 3153
Size: 56 6428
SacII CC_GC'GG
Cuts at: 3621 3621
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SapI GCTCTTCn'nnn_
Cuts at: 2783 3197 2783
Size: 414 6070
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SexAI A'CCwGG_T
Cuts at: 3534 3534
Size: 6484
SfcI C'TryA_G
Cuts at: 826 1045 2213 3635 3832 4967 5648 5839 826
Size: 219 1168 1422 197 1135 681 191 1471
Fragments arranged by size:
1471 1422 1168 1135 681 219 197 191
SfoI GGC'GCC
Cuts at: 2041 2314 2041
Size: 273 6211
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2999 4588 5489 5751 820
Size: 197 926 1056 1589 901 262 1553
Fragments arranged by size:
1589 1553 1056 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 3842 1132
Size: 2710 3774
SspI AAT'ATT
Cuts at: 5 52 4408 5
Size: 47 4356 2081
Fragments arranged by size:
4356 2081 47
StuI AGG'CCT
Cuts at: 1517 3317 1517
Size: 1800 4684
StyI C'CwwG_G
Cuts at: 513 1065 2015 3367 3923 513
Size: 552 950 1352 556 3074
Fragments arranged by size:
3074 1352 950 556 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3728 4511 4528 4681 4866 5311 325
Size: 1255 2148 783 17 153 185 445 1498
Fragments arranged by size:
2148 1498 1255 783 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2466 4730 6442
Size: 274 80 33 51 1443 489 2264 1712
Cuts at: 6442 96
Size: 138
Fragments arranged by size:
2264 1712 1443 489 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3407 3439 3471 3503 6444 6476 3407
Size: 32 32 32 2941 32 3415
Fragments arranged by size:
3415 2941 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3080 1316
Size: 1764 4720
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2453 2453
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 4613 4613
Size: 6484
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2320 2338 4292 276
Size: 53 83 186 936 786 18 1954 2468
Fragments arranged by size:
2468 1954 936 786 186 83 53 18
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI
PfoI PspXI PsrI RsrII SanDI SfiI SrfI SwaI