Restriction map for
pFN21AE2015
(Circular) (Six-base) MAPSORT of: pFN21AE2015.seq Check: 3784 from: 1 to: 6481
pFN21AE2015 6481 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2741 3824 1937
Size: 804 1083 4594
Fragments arranged by size:
4594 1083 804
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2871 4544 5928 6196 1632
Size: 1239 1673 1384 268 1917
Fragments arranged by size:
1917 1673 1384 1239 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6284
AflIII A'CryG_T
Cuts at: 1203 5380 6357 1203
Size: 4177 977 1327
Fragments arranged by size:
4177 1327 977
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 2359 5210 2359
Size: 2851 3630
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6481
AlwNI CAG_nnn'CTG
Cuts at: 2675 2687 4224 5796 2675
Size: 12 1537 1572 3360
Fragments arranged by size:
3360 1572 1537 12
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 2635 2924 2972 3124 3790 4016 4168 6277
Size: 1142 289 48 152 666 226 152 2109
Cuts at: 6277 1493
Size: 1697
Fragments arranged by size:
2109 1697 1142 666 289 226 152 152
48
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 3806 1870
Size: 73 1863 4545
Fragments arranged by size:
4545 1863 73
BamHI G'GATC_C
Cuts at: 3811 4178 3811
Size: 367 6114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3802 5257 618
Size: 325 217 627 2015 1455 1842
Fragments arranged by size:
2015 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2234 3800 1391
Size: 843 1566 4072
Fragments arranged by size:
4072 1566 843
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2804 2971 928
Size: 971 905 167 4438
Fragments arranged by size:
4438 971 905 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4672 4706 1903
Size: 34 2735 34 3678
Fragments arranged by size:
3678 2735 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4373 5583 4373
Size: 1210 5271
BclI T'GATC_A
Cuts at: 1548 2371 2410 3760 1548
Size: 823 39 1350 4269
Fragments arranged by size:
4269 1350 823 39
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3591 5092 136
Size: 107 122 71 631 605 1919 1501 1525
Fragments arranged by size:
1919 1525 1501 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6476 6476
Size: 6481
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 4542 5698 1790
Size: 2752 1156 2573
Fragments arranged by size:
2752 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4212 5170 442
Size: 416 53 83 399 24 2795 958 1753
Fragments arranged by size:
2795 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 5141 1239
Size: 910 2992 2579
Fragments arranged by size:
2992 2579 910
Bpu10I CC'TnA_GC
Cuts at: 2892 2892
Size: 6481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4606 5471 5769 4606
Size: 865 298 5318
Fragments arranged by size:
5318 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3051 3237 3417 3462 3486 3597 3627
Size: 617 1552 186 180 45 24 111 30
Cuts at: 3627 3678 3702 5144 882
Size: 51 24 1442 2219
Fragments arranged by size:
2219 1552 1442 617 186 180 111 51
45 30 24 24
BsaAI yAC'GTr
Cuts at: 493 1204 4272 493
Size: 711 3068 2702
Fragments arranged by size:
3068 2702 711
BsaBI GATnn'nnATC
Cuts at: 2376 6475 2376
Size: 4099 2382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4288 4670 5305 275
Size: 53 83 186 936 2755 382 635 1451
Fragments arranged by size:
2755 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2801 4914 5312 5586 5733 6144 2801
Size: 2113 398 274 147 411 3138
Fragments arranged by size:
3138 2113 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2723 2753 1178
Size: 30 165 30 1320 30 4906
Fragments arranged by size:
4906 1320 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2565 2997 3192 3420 3435 3444 3459 3480
Size: 1379 432 195 228 15 9 15 21
Cuts at: 3480 3513 3525 3534 3546 3555 3567 3576 3588
Size: 33 12 9 12 9 12 9 12
Cuts at: 3588 3615 3669 3696 3720 1186
Size: 27 54 27 24 3947
Fragments arranged by size:
3947 1379 432 228 195 54 33 27
27 24 21 15 15 12 12 12
12 9 9 9 9
BseYI C'CCAG_C
Cuts at: 5684 6180 5684
Size: 496 5985
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5908
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3931 4692 5720 1540
Size: 275 186 1930 761 1028 2301
Fragments arranged by size:
2301 1930 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2234 2311 4542 4627 5698 2234
Size: 77 2231 85 1071 3017
Fragments arranged by size:
3017 2231 1071 85 77
BsmI GAATG_Cn'
Cuts at: 3992 4085 3992
Size: 93 6388
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2311 3800 4542 4627 5698 1391
Size: 399 444 77 1489 742 85 1071 2174
Fragments arranged by size:
2174 1489 1071 742 444 399 85 77
BspEI T'CCGG_A
Cuts at: 6144 6144
Size: 6481
BspHI T'CATG_A
Cuts at: 2976 4370 6100 2976
Size: 1394 1730 3357
Fragments arranged by size:
3357 1730 1394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3836 844
Size: 2992 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4368 1394
Size: 429 2545 3507
Fragments arranged by size:
3507 2545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3148 4976 5150 65
Size: 1183 1900 1828 174 1396
Fragments arranged by size:
1900 1828 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5125 5312 1700
Size: 110 3315 187 2869
Fragments arranged by size:
3315 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6481
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 4541 5553 4541
Size: 1012 5469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3138 794
Size: 2344 4137
BstBI TT'CG_AA
Cuts at: 5285 5285
Size: 6481
BstEII G'GTnAC_C
Cuts at: 1184 2615 1184
Size: 1431 5050
BstXI CCAn_nnnn'nTGG
Cuts at: 2087 2087
Size: 6481
BstYI r'GATC_y
Cuts at: 1972 3811 4178 4563 4580 6032 6118 6130 6476
Size: 1839 367 385 17 1452 86 12 346
Cuts at: 6476 1972
Size: 1977
Fragments arranged by size:
1977 1839 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3172 3172
Size: 6481
BtgI C'CryG_G
Cuts at: 513 1065 2620 2929 3013 3607 513
Size: 552 1555 309 84 594 3387
Fragments arranged by size:
3387 1555 594 552 309 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5742
Fragments arranged by size:
5742 683 56
BtsI GCAGTG_nn'
Cuts at: 3625 3997 4791 4811 3625
Size: 372 794 20 5295
Fragments arranged by size:
5295 794 372 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 2767 3785 2767
Size: 1018 5463
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3928 4817 6446
Size: 54 1425 50 275 18 2098 889 1629
Cuts at: 6446 8
Size: 43
Fragments arranged by size:
2098 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3928 1537
Size: 275 2116 4090
Fragments arranged by size:
4090 2116 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2299 2581 4411 1353
Size: 946 282 1830 3423
Fragments arranged by size:
3423 1830 946 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5068 5442 5588 387
Size: 1064 138 3479 374 146 1280
Fragments arranged by size:
3479 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2232 2232
Size: 6481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2871 4544 5141 5928 6196 1239
Size: 393 517 722 1673 597 787 268 1524
Fragments arranged by size:
1673 1524 787 722 597 517 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2598 2598
Size: 6481
EcoO109I rG'GnC_Cy
Cuts at: 2591 2591
Size: 6481
EcoRI G'AATT_C
Cuts at: 2924 2972 3124 3790 4168 2924
Size: 48 152 666 378 5237
Fragments arranged by size:
5237 666 378 152 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2328 2328
Size: 6481
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 5628 1678
Size: 3950 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2384 2416 2669
Size: 32 135 32 773 32 7 32 253
Cuts at: 2669 2701 2723 2755 5122 5154 5196 5228 1373
Size: 32 22 32 2367 32 42 32 2626
Fragments arranged by size:
2626 2367 773 253 135 42 32 32
32 32 32 32 32 32 22 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2339 3825 4071 669
Size: 988 281 401 1486 246 3079
Fragments arranged by size:
3079 1486 988 401 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6481
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 4080 4080
Size: 6481
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 5595 5779 1305
Size: 684 3606 184 2007
Fragments arranged by size:
3606 2007 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4659
Fragments arranged by size:
4659 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2945 4439 4798 4957 518
Size: 648 1779 1494 359 159 2042
Fragments arranged by size:
2042 1779 1494 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2157 2622 3303 3318 3897 4574 5722 5967
Size: 551 465 681 15 579 677 1148 245
Cuts at: 5967 1606
Size: 2120
Fragments arranged by size:
2120 1148 681 677 579 551 465 245
15
NcoI C'CATG_G
Cuts at: 513 1065 2929 513
Size: 552 1864 4065
Fragments arranged by size:
4065 1864 552
NdeI CA'TA_TG
Cuts at: 387 2350 2389 387
Size: 1963 39 4479
Fragments arranged by size:
4479 1963 39
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 3839 5384 1132
Size: 97 2610 1545 2229
Fragments arranged by size:
2610 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 5380 5380
Size: 6481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2333 2907 1108
Size: 1225 574 4682
Fragments arranged by size:
4682 1225 574
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 6063 6095 4550
Size: 32 1481 32 4936
Fragments arranged by size:
4936 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2591 2591
Size: 6481
PshAI GACnn'nnGTC
Cuts at: 1613 2334 3165 1613
Size: 721 831 4929
Fragments arranged by size:
4929 831 721
PsiI TTA'TAA
Cuts at: 4051 4051
Size: 6481
PstI C_TGCA'G
Cuts at: 830 3833 830
Size: 3003 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
SacI G_AGCT'C
Cuts at: 2234 2234
Size: 6481
SacII CC_GC'GG
Cuts at: 2623 2623
Size: 6481
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SapI GCTCTTCn'nnn_
Cuts at: 2299 2299
Size: 6481
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 4729 6441 4729
Size: 1712 4769
SfcI C'TryA_G
Cuts at: 826 1045 2152 2428 2470 2742 3217 3313 3751
Size: 219 1107 276 42 272 475 96 438
Cuts at: 3751 3829 4964 5645 5836 826
Size: 78 1135 681 191 1471
Fragments arranged by size:
1471 1135 1107 681 475 438 276 272
219 191 96 78 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4585 5486 5748 820
Size: 197 926 2642 901 262 1553
Fragments arranged by size:
2642 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SspI AAT'ATT
Cuts at: 5 52 4405 5
Size: 47 4353 2081
Fragments arranged by size:
4353 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6481
StyI C'CwwG_G
Cuts at: 513 1065 2080 2296 2929 3920 513
Size: 552 1015 216 633 991 3074
Fragments arranged by size:
3074 1015 991 633 552 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3261 3441 3486 3510 3651 3702 3726
Size: 1255 1681 180 45 24 141 51 24
Cuts at: 3726 4508 4525 4678 4863 5308 325
Size: 782 17 153 185 445 1498
Fragments arranged by size:
1681 1498 1255 782 445 185 180 153
141 51 45 24 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4727 6439 96
Size: 274 80 33 51 1443 2750 1712 138
Fragments arranged by size:
2750 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6441 6473 6441
Size: 32 6449
Tth111I GACn'n_nGTC
Cuts at: 1316 2178 1316
Size: 862 5619
XbaI T'CTAG_A
Cuts at: 3817 3817
Size: 6481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 2330
Size: 6481
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6481
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 3124 4610 3124
Size: 1486 4995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SanDI SexAI SfiI SfoI SrfI SwaI