Restriction map for
pFN21AE2011
(Circular) (Six-base) MAPSORT of: pFN21AE2011.seq Check: 1690 from: 1 to: 6475
pFN21AE2011 6475 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4285 278
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2928 3818 1937
Size: 991 890 4594
Fragments arranged by size:
4594 991 890
Acc65I G'GTAC_C
Cuts at: 3596 3796 3596
Size: 200 6275
AclI AA'CG_TT
Cuts at: 1563 4602 4975 1563
Size: 3039 373 3063
Fragments arranged by size:
3063 3039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 2837 3331 4538 5922 6190 1632
Size: 494 711 494 1207 1384 268 1917
Fragments arranged by size:
1917 1384 1207 711 494 494 268
AfeI AGC'GCT
Cuts at: 2651 3368 2651
Size: 717 5758
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6278
AflIII A'CryG_T
Cuts at: 1203 3579 5374 6351 1203
Size: 2376 1795 977 1327
Fragments arranged by size:
2376 1795 1327 977
AgeI A'CCGG_T
Cuts at: 2542 5306 2542
Size: 2764 3711
AhdI GACnn_n'nnGTC
Cuts at: 5204 5204
Size: 6475
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3287 1184
Size: 2103 4372
AlwNI CAG_nnn'CTG
Cuts at: 2254 3298 4218 5790 2254
Size: 1044 920 1572 2939
Fragments arranged by size:
2939 1572 1044 920
ApaLI G'TGCA_C
Cuts at: 2439 4532 5688 2439
Size: 2093 1156 3226
Fragments arranged by size:
3226 2093 1156
ApoI r'AATT_y
Cuts at: 1493 3784 4010 4162 6271 1493
Size: 2291 226 152 2109 1697
Fragments arranged by size:
2291 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5029 160
Size: 872 3997 1606
Fragments arranged by size:
3997 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
AvaI C'yCGr_G
Cuts at: 1870 1943 2717 3014 3800 1870
Size: 73 774 297 786 4545
Fragments arranged by size:
4545 786 774 297 73
BamHI G'GATC_C
Cuts at: 3805 4172 3805
Size: 367 6108
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3596 3796 5251 618
Size: 325 217 627 1809 200 1455 1842
Fragments arranged by size:
1842 1809 1455 627 325 217 200
BanII G_rGCy'C
Cuts at: 1391 3101 3521 3794 1391
Size: 1710 420 273 4072
Fragments arranged by size:
4072 1710 420 273
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 2910 928
Size: 971 240 771 4493
Fragments arranged by size:
4493 971 771 240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4666 4700 1903
Size: 34 2729 34 3678
Fragments arranged by size:
3678 2729 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4367 5577 4367
Size: 1210 5265
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6475
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3042 5086 136
Size: 107 122 71 631 605 1370 2044 1525
Fragments arranged by size:
2044 1525 1370 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2935 3610 6470 2935
Size: 675 2860 2940
Fragments arranged by size:
2940 2860 675
BlpI GC'TnA_GC
Cuts at: 3892 3892
Size: 6475
Bme1580I G_kGCm'C
Cuts at: 1790 2443 3047 4536 5692 1790
Size: 653 604 1489 1156 2573
Fragments arranged by size:
2573 1489 1156 653 604
BmgBI CAC'GTC
Cuts at: 1318 3433 1318
Size: 2115 4360
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2306 3118 4206
Size: 416 53 83 399 24 889 812 1088
Cuts at: 4206 5164 442
Size: 958 1753
Fragments arranged by size:
1753 1088 958 889 812 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6475
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3244 3469 3244
Size: 225 6250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2268 2501 3048 3230 3641 5135 1239
Size: 1016 13 233 547 182 411 1494 2579
Fragments arranged by size:
2579 1494 1016 547 411 233 182 13
Bpu10I CC'TnA_GC
Cuts at: 3095 3095
Size: 6475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4600 5465 5763 4600
Size: 865 298 5312
Fragments arranged by size:
5312 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2386 2759 3015 5138 882
Size: 617 887 373 256 2123 2219
Fragments arranged by size:
2219 2123 887 617 373 256
BsaAI yAC'GTr
Cuts at: 493 1204 4266 493
Size: 711 3062 2702
Fragments arranged by size:
3062 2702 711
BsaBI GATnn'nnATC
Cuts at: 6469 6469
Size: 6475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3468 4282 4664 5299
Size: 53 83 186 936 1935 814 382 635
Cuts at: 5299 275
Size: 1451
Fragments arranged by size:
1935 1451 936 814 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2542 3631 3764 4908 5306 5580 5727 6138 2542
Size: 1089 133 1144 398 274 147 411 2879
Fragments arranged by size:
2879 1144 1089 411 398 274 147 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2366 2396 3209 3224 3239
Size: 30 165 30 963 30 813 15 15
Cuts at: 3239 3254 3269 3485 3515 1178
Size: 15 15 216 30 4138
Fragments arranged by size:
4138 963 813 216 165 30 30 30
30 15 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2408 2870 2873 3203 3233 3260 3326 1186
Size: 1222 462 3 330 30 27 66 4335
Fragments arranged by size:
4335 1222 462 330 66 30 27 3
BseYI C'CCAG_C
Cuts at: 3298 3704 5678 6174 3298
Size: 406 1974 496 3599
Fragments arranged by size:
3599 1974 496 406
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5902
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3037 3166 3925 4686 5714 1540
Size: 275 186 1036 129 759 761 1028 2301
Fragments arranged by size:
2301 1036 1028 761 759 275 186 129
BsiHKAI G_wGCw'C
Cuts at: 2443 4536 4621 5692 2443
Size: 2093 85 1071 3226
Fragments arranged by size:
3226 2093 1071 85
BsmI GAATG_Cn'
Cuts at: 3566 3986 4079 3566
Size: 420 93 5962
Fragments arranged by size:
5962 420 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3206 3206
Size: 6475
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2443 3047 3101 3521 3794 4536 4621
Size: 399 653 604 54 420 273 742 85
Cuts at: 4621 5692 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 653 604 420 399 273
85 54
BspEI T'CCGG_A
Cuts at: 3631 6138 3631
Size: 2507 3968
BspHI T'CATG_A
Cuts at: 2799 4364 6094 2799
Size: 1565 1730 3180
Fragments arranged by size:
3180 1730 1565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3830 844
Size: 2986 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2863 4362 1394
Size: 429 1040 1499 3507
Fragments arranged by size:
3507 1499 1040 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4970 5144 65
Size: 1183 3722 174 1396
Fragments arranged by size:
3722 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2542 5119 5306 1700
Size: 110 732 2577 187 2869
Fragments arranged by size:
2869 2577 732 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6475
BssSI C'ACGA_G
Cuts at: 2453 4535 5547 2453
Size: 2082 1012 3381
Fragments arranged by size:
3381 2082 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6475
BstBI TT'CG_AA
Cuts at: 5279 5279
Size: 6475
BstEII G'GTnAC_C
Cuts at: 1184 2545 2810 1184
Size: 1361 265 4849
Fragments arranged by size:
4849 1361 265
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6475
BstYI r'GATC_y
Cuts at: 1972 2712 2886 2935 2982 3009 3610 3805 4172
Size: 740 174 49 47 27 601 195 367
Cuts at: 4172 4557 4574 6026 6112 6124 6470 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 740 601 385 367 346 195
174 86 49 47 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2462 2462
Size: 6475
BtgI C'CryG_G
Cuts at: 513 1065 2004 2262 2625 2691 3031 3160 3513
Size: 552 939 258 363 66 340 129 353
Cuts at: 3513 3533 513
Size: 20 3455
Fragments arranged by size:
3455 939 552 363 353 340 258 129
66 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5736
Fragments arranged by size:
5736 683 56
BtsI GCAGTG_nn'
Cuts at: 3991 4785 4805 3991
Size: 794 20 5661
Fragments arranged by size:
5661 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6440
DraI TTT'AAA
Cuts at: 3779 3779
Size: 6475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3203 5482 809
Size: 507 1887 2279 1802
Fragments arranged by size:
2279 1887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2368 2971
Size: 54 1425 50 275 18 195 343 603
Cuts at: 2971 3034 3163 3922 4811 6440 8
Size: 63 129 759 889 1629 43
Fragments arranged by size:
1629 1425 889 759 603 343 275 195
129 63 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3034 3163 3922 1537
Size: 275 1222 129 759 4090
Fragments arranged by size:
4090 1222 759 275 129
EarI CTCTTCn'nnn_
Cuts at: 1353 2657 3671 4405 1353
Size: 1304 1014 734 3423
Fragments arranged by size:
3423 1304 1014 734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2076 5062 5436 5582 387
Size: 1064 138 487 2986 374 146 1280
Fragments arranged by size:
2986 1280 1064 487 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2255 2268 2501 2837 3048 3230
Size: 393 494 129 13 233 336 211 182
Cuts at: 3230 3331 3641 4538 5135 5922 6190 1239
Size: 101 310 897 597 787 268 1524
Fragments arranged by size:
1524 897 787 597 494 393 336 310
268 233 211 182 129 101 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2720 3346 2720
Size: 626 5849
EcoO109I rG'GnC_Cy
Cuts at: 3070 3112 3205 3070
Size: 42 93 6340
Fragments arranged by size:
6340 93 42
EcoRI G'AATT_C
Cuts at: 3784 4162 3784
Size: 378 6097
FspI TGC'GCA
Cuts at: 4981 4981
Size: 6475
HaeII r_GCGC'y
Cuts at: 1678 2653 3370 5622 1678
Size: 975 717 2252 2531
Fragments arranged by size:
2531 2252 975 717
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 3012 3044 3134
Size: 32 135 32 794 32 614 32 90
Cuts at: 3134 3166 3239 3271 3485 3517 5116 5148 5190
Size: 32 73 32 214 32 1599 32 42
Cuts at: 5190 5222 1373
Size: 32 2626
Fragments arranged by size:
2626 1599 794 614 214 135 90 73
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2728 2746 3819 4065 669
Size: 988 281 790 18 1073 246 3079
Fragments arranged by size:
3079 1073 988 790 281 246 18
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6475
HpaI GTT'AAC
Cuts at: 4065 4065
Size: 6475
KpnI G_GTAC'C
Cuts at: 3600 3800 3600
Size: 200 6275
MfeI C'AATT_G
Cuts at: 4074 4074
Size: 6475
MluI A'CGCG_T
Cuts at: 6351 6351
Size: 6475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2511 3366 5589 5773 1305
Size: 1206 855 2223 184 2007
Fragments arranged by size:
2223 2007 1206 855 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 2370 2973 10
Size: 54 1425 343 195 343 603 3512
Fragments arranged by size:
3512 1425 603 343 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4433 4792 4951 518
Size: 648 3267 359 159 2042
Fragments arranged by size:
3267 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3033 3162 3891 4568 5716 5961 1606
Size: 1427 129 729 677 1148 245 2120
Fragments arranged by size:
2120 1427 1148 729 677 245 129
NcoI C'CATG_G
Cuts at: 513 1065 2004 2691 3533 513
Size: 552 939 687 842 3455
Fragments arranged by size:
3455 939 842 687 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6475
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6475
NotI GC'GGCC_GC
Cuts at: 3163 3922 3163
Size: 759 5716
NspI r_CATG'y
Cuts at: 1132 1229 2500 3833 5378 1132
Size: 97 1271 1333 1545 2229
Fragments arranged by size:
2229 1545 1333 1271 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6475
PciI A'CATG_T
Cuts at: 5374 5374
Size: 6475
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 3534 1108
Size: 1269 1157 4049
Fragments arranged by size:
4049 1269 1157
PfoI T'CCnGG_A
Cuts at: 2253 3207 2253
Size: 954 5521
PmeI GTTT'AAAC
Cuts at: 3779 3779
Size: 6475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4544 4576 6057 6089 4544
Size: 32 1481 32 4930
Fragments arranged by size:
4930 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3070 3205 3070
Size: 135 6340
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6475
PsiI TTA'TAA
Cuts at: 4045 4045
Size: 6475
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 6443
PstI C_TGCA'G
Cuts at: 830 2168 3325 3827 830
Size: 1338 1157 502 3478
Fragments arranged by size:
3478 1338 1157 502
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6475
SacII CC_GC'GG
Cuts at: 3034 3163 3034
Size: 129 6346
SalI G'TCGA_C
Cuts at: 1936 3817 1936
Size: 1881 4594
SbfI CC_TGCA'GG
Cuts at: 3827 3827
Size: 6475
ScaI AGT'ACT
Cuts at: 4723 6435 4723
Size: 1712 4763
SexAI A'CCwGG_T
Cuts at: 2376 2376
Size: 6475
SfcI C'TryA_G
Cuts at: 826 1045 2164 2405 3321 3823 4958 5639 5830
Size: 219 1119 241 916 502 1135 681 191
Cuts at: 5830 826
Size: 1471
Fragments arranged by size:
1471 1135 1119 916 681 502 241 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6475
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6475
SmaI CCC'GGG
Cuts at: 1872 3802 1872
Size: 1930 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 4579 5480 5742 820
Size: 197 926 774 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 774 262 197
SnaBI TAC'GTA
Cuts at: 493 4266 493
Size: 3773 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6475
SphI G_CATG'C
Cuts at: 1132 3833 1132
Size: 2701 3774
SspI AAT'ATT
Cuts at: 5 52 4399 5
Size: 47 4347 2081
Fragments arranged by size:
4347 2081 47
StuI AGG'CCT
Cuts at: 1517 2227 1517
Size: 710 5765
StyI C'CwwG_G
Cuts at: 513 1065 2004 2691 2769 3115 3533 3914 513
Size: 552 939 687 78 346 418 381 3074
Fragments arranged by size:
3074 939 687 552 418 381 346 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4502 4519 4672 4857 5302 325
Size: 1255 2922 17 153 185 445 1498
Fragments arranged by size:
2922 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3225 3420 4721
Size: 274 80 33 51 1443 1248 195 1301
Cuts at: 4721 6433 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1301 1248 274 195 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2433 2465 2765 2797 3224 3256 6435 6467 2433
Size: 32 300 32 427 32 3179 32 2441
Fragments arranged by size:
3179 2441 427 300 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2378 1316
Size: 1062 5413
XbaI T'CTAG_A
Cuts at: 3811 3811
Size: 6475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2230 2230
Size: 6475
XhoI C'TCGA_G
Cuts at: 1943 2717 1943
Size: 774 5701
XmaI C'CCGG_G
Cuts at: 1870 3800 1870
Size: 1930 4545
XmnI GAAnn'nnTTC
Cuts at: 4604 4604
Size: 6475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4283 276
Size: 53 83 186 936 2749 2468
Fragments arranged by size:
2749 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SacII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PvuII RsrII SacI SanDI SapI
SfiI SfoI SrfI SwaI