Restriction map for
pFN21AE2005
(Circular) (Six-base) MAPSORT of: pFN21AE2005.seq Check: 6575 from: 1 to: 6448
pFN21AE2005 6448 bp
With 182 enzymes: SgfI *
March 28, 2012 12:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2032 3592 3749 2032
Size: 1560 157 4731
Fragments arranged by size:
4731 1560 157
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3177 4258 278
Size: 53 83 186 936 1641 1081 2468
Fragments arranged by size:
2468 1641 1081 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 3791 1937
Size: 883 971 4594
Fragments arranged by size:
4594 971 883
Acc65I G'GTAC_C
Cuts at: 3769 3769
Size: 6448
AclI AA'CG_TT
Cuts at: 1563 4575 4948 1563
Size: 3012 373 3063
Fragments arranged by size:
3063 3012 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2570 4511 5895 6163 1632
Size: 938 1941 1384 268 1917
Fragments arranged by size:
1941 1917 1384 938 268
AfeI AGC'GCT
Cuts at: 2678 2678
Size: 6448
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6251
AflIII A'CryG_T
Cuts at: 1203 2652 5347 6324 1203
Size: 1449 2695 977 1327
Fragments arranged by size:
2695 1449 1327 977
AgeI A'CCGG_T
Cuts at: 5279 5279
Size: 6448
AhdI GACnn_n'nnGTC
Cuts at: 5177 5177
Size: 6448
AleI CACnn'nnGTG
Cuts at: 1166 3374 3743 1166
Size: 2208 369 3871
Fragments arranged by size:
3871 2208 369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6448
AlwNI CAG_nnn'CTG
Cuts at: 4191 5763 4191
Size: 1572 4876
ApaI G_GGCC'C
Cuts at: 2021 2564 2021
Size: 543 5905
ApaLI G'TGCA_C
Cuts at: 2287 4505 5661 2287
Size: 2218 1156 3074
Fragments arranged by size:
3074 2218 1156
ApoI r'AATT_y
Cuts at: 1493 3757 3983 4135 6244 1493
Size: 2264 226 152 2109 1697
Fragments arranged by size:
2264 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5002 160
Size: 872 3970 1606
Fragments arranged by size:
3970 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 2089 2481 2488 2564 2587 2780
Size: 73 71 75 392 7 76 23 193
Cuts at: 2780 3247 3773 1870
Size: 467 526 4545
Fragments arranged by size:
4545 526 467 392 193 76 75 73
71 23 7
BamHI G'GATC_C
Cuts at: 3778 4145 3778
Size: 367 6081
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2452 2855 3769 5224 618
Size: 325 217 627 665 403 914 1455 1842
Fragments arranged by size:
1842 1455 914 665 627 403 325 217
BanII G_rGCy'C
Cuts at: 1391 2021 2054 2478 2489 2564 3144 3342 3767
Size: 630 33 424 11 75 580 198 425
Cuts at: 3767 1391
Size: 4072
Fragments arranged by size:
4072 630 580 425 424 198 75 33
11
BbeI G_GCGC'C
Cuts at: 2456 2859 2456
Size: 403 6045
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2932 3104 3293 928
Size: 971 1033 172 189 4083
Fragments arranged by size:
4083 1033 971 189 172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2119 2153 4639 4673 1903
Size: 34 50 34 98 34 2486 34 3678
Fragments arranged by size:
3678 2486 98 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4340 5550 4340
Size: 1210 5238
BclI T'GATC_A
Cuts at: 1548 3654 1548
Size: 2106 4342
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2521 2705 3089
Size: 107 122 71 631 605 849 184 384
Cuts at: 3089 5059 136
Size: 1970 1525
Fragments arranged by size:
1970 1525 849 631 605 384 184 122
107 71
BglII A'GATC_T
Cuts at: 3510 3525 6443 3510
Size: 15 2918 3515
Fragments arranged by size:
3515 2918 15
BlpI GC'TnA_GC
Cuts at: 3865 3865
Size: 6448
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2291 2564 4509 5665 1790
Size: 231 270 273 1945 1156 2573
Fragments arranged by size:
2573 1945 1156 273 270 231
BmgBI CAC'GTC
Cuts at: 1318 2653 3234 1318
Size: 1335 581 4532
Fragments arranged by size:
4532 1335 581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3724 4179 5137
Size: 416 53 83 399 24 2307 455 958
Cuts at: 5137 442
Size: 1753
Fragments arranged by size:
2307 1753 958 455 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 6448
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2680 2710 2936 3335 5108 1239
Size: 1441 30 226 399 1773 2579
Fragments arranged by size:
2579 1773 1441 399 226 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 4573 5438 5736 2333
Size: 2240 865 298 3045
Fragments arranged by size:
3045 2240 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2305 5111 882
Size: 617 806 2806 2219
Fragments arranged by size:
2806 2219 806 617
BsaAI yAC'GTr
Cuts at: 493 1204 2605 3160 4239 493
Size: 711 1401 555 1079 2702
Fragments arranged by size:
2702 1401 1079 711 555
BsaBI GATnn'nnATC
Cuts at: 6442 6442
Size: 6448
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2453 2856 3174 4255
Size: 53 83 186 936 920 403 318 1081
Cuts at: 4255 4637 5272 275
Size: 382 635 1451
Fragments arranged by size:
1451 1081 936 920 635 403 382 318
186 83 53
BsaWI w'CCGG_w
Cuts at: 3273 4881 5279 5553 5700 6111 3273
Size: 1608 398 274 147 411 3610
Fragments arranged by size:
3610 1608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6223
Fragments arranged by size:
6223 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6448
BseYI C'CCAG_C
Cuts at: 3684 5651 6147 3684
Size: 1967 496 3985
Fragments arranged by size:
3985 1967 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2620 2914 3628 1163
Size: 573 884 294 714 3983
Fragments arranged by size:
3983 884 714 573 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2516 2878 3898 4659 5687 1540
Size: 275 186 515 362 1020 761 1028 2301
Fragments arranged by size:
2301 1028 1020 761 515 362 275 186
BsiHKAI G_wGCw'C
Cuts at: 2270 2291 2357 2544 2745 2924 3144 3201 4509
Size: 21 66 187 201 179 220 57 1308
Cuts at: 4509 4594 5665 2270
Size: 85 1071 3053
Fragments arranged by size:
3053 1308 1071 220 201 187 179 85
66 57 21
BsmI GAATG_Cn'
Cuts at: 3959 4052 3959
Size: 93 6355
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2054 2270 2291 2357 2478 2489
Size: 399 231 33 216 21 66 121 11
Cuts at: 2489 2544 2564 2745 2924 3144 3201 3342 3767
Size: 55 20 181 179 220 57 141 425
Cuts at: 3767 4509 4594 5665 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 425 399 231 220 216
181 179 141 121 85 66 57 55
33 21 20 11
BspEI T'CCGG_A
Cuts at: 3273 6111 3273
Size: 2838 3610
BspHI T'CATG_A
Cuts at: 4337 6067 4337
Size: 1730 4718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2032 2287 3592 3749 3803 844
Size: 1188 255 1305 157 54 3489
Fragments arranged by size:
3489 1305 1188 255 157 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2632 4335 1394
Size: 429 809 1703 3507
Fragments arranged by size:
3507 1703 809 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4943 5117 65
Size: 1183 3695 174 1396
Fragments arranged by size:
3695 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2852 2924 3017 3188 3364 3643 5092
Size: 110 1042 72 93 171 176 279 1449
Cuts at: 5092 5279 1700
Size: 187 2869
Fragments arranged by size:
2869 1449 1042 279 187 176 171 110
93 72
BsrGI T'GTAC_A
Cuts at: 96 3354 96
Size: 3258 3190
BssHII G'CGCG_C
Cuts at: 1925 2532 2625 1925
Size: 607 93 5748
Fragments arranged by size:
5748 607 93
BssSI C'ACGA_G
Cuts at: 2053 2177 2210 3035 4508 5520 2053
Size: 124 33 825 1473 1012 2981
Fragments arranged by size:
2981 1473 1012 825 124 33
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2871 2928 794
Size: 2077 57 4314
Fragments arranged by size:
4314 2077 57
BstBI TT'CG_AA
Cuts at: 5252 5252
Size: 6448
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6448
BstXI CCAn_nnnn'nTGG
Cuts at: 2080 2170 3745 2080
Size: 90 1575 4783
Fragments arranged by size:
4783 1575 90
BstYI r'GATC_y
Cuts at: 1972 2118 3423 3510 3525 3720 3778 4145 4530
Size: 146 1305 87 15 195 58 367 385
Cuts at: 4530 4547 5999 6085 6097 6443 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1305 385 367 346 195 146
87 86 58 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 2073 2242 2826 3504 3706 513
Size: 552 1008 169 584 678 202 3255
Fragments arranged by size:
3255 1008 678 584 552 202 169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2851 487
Size: 683 56 1625 4084
Fragments arranged by size:
4084 1625 683 56
BtsI GCAGTG_nn'
Cuts at: 2691 3964 4758 4778 2691
Size: 1273 794 20 4361
Fragments arranged by size:
4361 1273 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6413
DraI TTT'AAA
Cuts at: 3752 3752
Size: 6448
DraIII CAC_nnn'GTG
Cuts at: 2605 2605
Size: 6448
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3589 5455 809
Size: 507 2273 1866 1802
Fragments arranged by size:
2273 1866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2245 2513 2697
Size: 54 1425 50 275 18 415 268 184
Cuts at: 2697 2887 2967 3012 3366 3486 3645 3895 4784
Size: 190 80 45 354 120 159 250 889
Cuts at: 4784 6413 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 415 354 275 268 250
190 184 159 120 80 54 50 45
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2513 3895 1537
Size: 275 701 1382 4090
Fragments arranged by size:
4090 1382 701 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 2852 3347 3623 4378 1353
Size: 1253 246 495 276 755 3423
Fragments arranged by size:
3423 1253 755 495 276 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2831 5035 5409 5555 387
Size: 1064 138 1242 2204 374 146 1280
Fragments arranged by size:
2204 1280 1242 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3142 3142
Size: 6448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2570 2680 2710 2936 3335 4511 5108
Size: 393 938 110 30 226 399 1176 597
Cuts at: 5108 5895 6163 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1176 938 787 597 399 393 268
226 110 30
EcoO109I rG'GnC_Cy
Cuts at: 2018 2560 2561 2018
Size: 542 1 5905
Fragments arranged by size:
5905 542 1
EcoRI G'AATT_C
Cuts at: 3757 4135 3757
Size: 378 6070
FspI TGC'GCA
Cuts at: 3122 4954 3122
Size: 1832 4616
HaeII r_GCGC'y
Cuts at: 1678 2014 2089 2456 2680 2733 2788 2859 5595
Size: 336 75 367 224 53 55 71 2736
Cuts at: 5595 1678
Size: 2531
Fragments arranged by size:
2736 2531 367 336 224 75 71 55
53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5089 5121 5163 5195 1373
Size: 32 135 32 3517 32 42 32 2626
Fragments arranged by size:
3517 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3178 3792 4038 669
Size: 988 281 1240 614 246 3079
Fragments arranged by size:
3079 1240 988 614 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6448
HpaI GTT'AAC
Cuts at: 4038 4038
Size: 6448
KasI G'GCGC_C
Cuts at: 2452 2855 2452
Size: 403 6045
KpnI G_GTAC'C
Cuts at: 3773 3773
Size: 6448
MfeI C'AATT_G
Cuts at: 4047 4047
Size: 6448
MluI A'CGCG_T
Cuts at: 6324 6324
Size: 6448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5562 5746 1305
Size: 4257 184 2007
Fragments arranged by size:
4257 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2699 2969 10
Size: 54 1425 343 867 270 3489
Fragments arranged by size:
3489 1425 867 343 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2078 3374 3743 4406 4765 4924 518
Size: 648 912 1296 369 663 359 159 2042
Fragments arranged by size:
2042 1296 912 663 648 369 359 159
MspA1I CmG'CkG
Cuts at: 1606 2224 2506 2521 2548 2886 3558 3663 3864
Size: 618 282 15 27 338 672 105 201
Cuts at: 3864 4541 5689 5934 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 672 618 338 282 245
201 105 27 15
NaeI GCC'GGC
Cuts at: 2854 3019 3366 2854
Size: 165 347 5936
Fragments arranged by size:
5936 347 165
NarI GG'CG_CC
Cuts at: 2453 2856 2453
Size: 403 6045
NcoI C'CATG_G
Cuts at: 513 1065 2073 513
Size: 552 1008 4888
Fragments arranged by size:
4888 1008 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6448
NgoMIV G'CCGG_C
Cuts at: 2852 3017 3364 2852
Size: 165 347 5936
Fragments arranged by size:
5936 347 165
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6448
NotI GC'GGCC_GC
Cuts at: 3895 3895
Size: 6448
NspI r_CATG'y
Cuts at: 1132 1229 3806 5351 1132
Size: 97 2577 1545 2229
Fragments arranged by size:
2577 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6448
PciI A'CATG_T
Cuts at: 5347 5347
Size: 6448
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 1108
Size: 1497 4951
PfoI T'CCnGG_A
Cuts at: 2904 2904
Size: 6448
PmeI GTTT'AAAC
Cuts at: 3752 3752
Size: 6448
PmlI CAC'GTG
Cuts at: 2605 2605
Size: 6448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4517 4549 6030 6062 4517
Size: 32 1481 32 4903
Fragments arranged by size:
4903 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6448
PsiI TTA'TAA
Cuts at: 4018 4018
Size: 6448
PspOMI G'GGCC_C
Cuts at: 2017 2560 2017
Size: 543 5905
PstI C_TGCA'G
Cuts at: 830 2043 3591 3800 830
Size: 1213 1548 209 3478
Fragments arranged by size:
3478 1548 1213 209
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6448
SacI G_AGCT'C
Cuts at: 3144 3144
Size: 6448
SalI G'TCGA_C
Cuts at: 1936 3790 1936
Size: 1854 4594
SapI GCTCTTCn'nnn_
Cuts at: 2606 3347 3623 2606
Size: 741 276 5431
Fragments arranged by size:
5431 741 276
SbfI CC_TGCA'GG
Cuts at: 2043 3800 2043
Size: 1757 4691
ScaI AGT'ACT
Cuts at: 4696 6408 4696
Size: 1712 4736
SexAI A'CCwGG_T
Cuts at: 3002 3219 3002
Size: 217 6231
SfcI C'TryA_G
Cuts at: 826 1045 2039 3587 3796 4931 5612 5803 826
Size: 219 994 1548 209 1135 681 191 1471
Fragments arranged by size:
1548 1471 1135 994 681 219 209 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2521 2521
Size: 6448
SfoI GGC'GCC
Cuts at: 2454 2857 2454
Size: 403 6045
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6448
SgrAI Cr'CCGG_yG
Cuts at: 1700 2852 1700
Size: 1152 5296
SmaI CCC'GGG
Cuts at: 1872 2016 2091 2483 2566 2589 3775 1872
Size: 144 75 392 83 23 1186 4545
Fragments arranged by size:
4545 1186 392 144 83 75 23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 4552 5453 5715 820
Size: 197 926 405 2204 901 262 1553
Fragments arranged by size:
2204 1553 926 901 405 262 197
SnaBI TAC'GTA
Cuts at: 493 4239 493
Size: 3746 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6448
SphI G_CATG'C
Cuts at: 1132 3806 1132
Size: 2674 3774
SrfI GCCC'GGGC
Cuts at: 2016 2091 2016
Size: 75 6373
SspI AAT'ATT
Cuts at: 5 52 4372 5
Size: 47 4320 2081
Fragments arranged by size:
4320 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6448
StyI C'CwwG_G
Cuts at: 513 1065 2073 3887 513
Size: 552 1008 1814 3074
Fragments arranged by size:
3074 1814 1008 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2974 4475 4492 4645 4830 5275 325
Size: 1255 1394 1501 17 153 185 445 1498
Fragments arranged by size:
1501 1498 1394 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 3354 4694
Size: 274 80 33 51 1443 678 699 1340
Cuts at: 4694 6406 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1340 699 678 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6408 6440 6408
Size: 32 6416
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6448
XbaI T'CTAG_A
Cuts at: 3784 3784
Size: 6448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 3109 3502 2071
Size: 1038 393 5017
Fragments arranged by size:
5017 1038 393
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6448
XmaI C'CCGG_G
Cuts at: 1870 2014 2089 2481 2564 2587 3773 1870
Size: 144 75 392 83 23 1186 4545
Fragments arranged by size:
4545 1186 392 144 83 75 23
XmnI GAAnn'nnTTC
Cuts at: 4577 4577
Size: 6448
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3175 4256 276
Size: 53 83 186 936 1641 1081 2468
Fragments arranged by size:
2468 1641 1081 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PpuMI PspXI PsrI PvuII RsrII
SacII SanDI SwaI