Restriction map for
pFN21AE2004
(Circular) (Six-base) MAPSORT of: pFN21AE2004.seq Check: 3574 from: 1 to: 6442
pFN21AE2004 6442 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3583 3785 1937
Size: 1646 202 4594
Fragments arranged by size:
4594 1646 202
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 3741 4569 4942 1563
Size: 2178 828 373 3063
Fragments arranged by size:
3063 2178 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2341 2810 2937 3674 4505 5889 6157 1632
Size: 709 469 127 737 831 1384 268 1917
Fragments arranged by size:
1917 1384 831 737 709 469 268 127
AfeI AGC'GCT
Cuts at: 2507 2507
Size: 6442
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 2244 5341 6318 1203
Size: 1041 3097 977 1327
Fragments arranged by size:
3097 1327 1041 977
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 2072 3572 1166
Size: 906 1500 4036
Fragments arranged by size:
4036 1500 906
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2090 1184
Size: 906 5536
AlwNI CAG_nnn'CTG
Cuts at: 3070 3487 3607 4185 5757 3070
Size: 417 120 578 1572 3755
Fragments arranged by size:
3755 1572 578 417 120
ApaI G_GGCC'C
Cuts at: 2232 2981 3443 2232
Size: 749 462 5231
Fragments arranged by size:
5231 749 462
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 3751 3977 4129 6238 1493
Size: 2258 226 152 2109 1697
Fragments arranged by size:
2258 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 2224 2281 3767 1870
Size: 73 281 57 1486 4545
Fragments arranged by size:
4545 1486 281 73 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2838 2871 2838
Size: 33 6409
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2277 3074 3763 5218 618
Size: 325 217 627 490 797 689 1455 1842
Fragments arranged by size:
1842 1455 797 689 627 490 325 217
BanII G_rGCy'C
Cuts at: 1391 2043 2232 2604 2981 3264 3419 3443 3761
Size: 652 189 372 377 283 155 24 318
Cuts at: 3761 1391
Size: 4072
Fragments arranged by size:
4072 652 377 372 318 283 189 155
24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3031 928
Size: 971 1132 4339
Fragments arranged by size:
4339 1132 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4633 4667 1903
Size: 34 2696 34 3678
Fragments arranged by size:
3678 2696 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2839 4334 5544 2839
Size: 1495 1210 3737
Fragments arranged by size:
3737 1495 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6442
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2018 2418 5053
Size: 107 122 71 631 605 346 400 2635
Cuts at: 5053 136
Size: 1525
Fragments arranged by size:
2635 1525 631 605 400 346 122 107
71
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 3263 3611 3859 3263
Size: 348 248 5846
Fragments arranged by size:
5846 348 248
Bme1580I G_kGCm'C
Cuts at: 1790 2232 2280 2981 3079 3443 4503 5659 1790
Size: 442 48 701 98 364 1060 1156 2573
Fragments arranged by size:
2573 1156 1060 701 442 364 98 48
BmgBI CAC'GTC
Cuts at: 1318 3145 1318
Size: 1827 4615
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4173 5131 442
Size: 416 53 83 399 24 2756 958 1753
Fragments arranged by size:
2756 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3010 3010
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2588 5102 1239
Size: 1349 2514 2579
Fragments arranged by size:
2579 2514 1349
Bpu10I CC'TnA_GC
Cuts at: 2390 3674 2390
Size: 1284 5158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2079 2603 3509 4567 5432 5730 2079
Size: 524 906 1058 865 298 2791
Fragments arranged by size:
2791 1058 906 865 524 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2300 5105 882
Size: 617 801 2805 2219
Fragments arranged by size:
2805 2219 801 617
BsaAI yAC'GTr
Cuts at: 493 1204 3274 3292 4233 493
Size: 711 2070 18 941 2702
Fragments arranged by size:
2702 2070 941 711 18
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4249 4631 5266 275
Size: 53 83 186 936 2716 382 635 1451
Fragments arranged by size:
2716 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2382 2538 4875 5273 5547 5694 6105 2382
Size: 156 2337 398 274 147 411 2719
Fragments arranged by size:
2719 2337 411 398 274 156 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3281 3311 1178
Size: 30 165 30 1878 30 4309
Fragments arranged by size:
4309 1878 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2936 2939 2983 3248 3289 3545 3647 1186
Size: 1750 3 44 265 41 256 102 3981
Fragments arranged by size:
3981 1750 265 256 102 44 41 3
BseYI C'CCAG_C
Cuts at: 2339 2349 2572 2898 5645 6141 2339
Size: 10 223 326 2747 496 2640
Fragments arranged by size:
2747 2640 496 326 223 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2050 2063 2750 3296 1163
Size: 573 314 13 687 546 4309
Fragments arranged by size:
4309 687 573 546 314 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2841 3892 4653 5681 1540
Size: 275 186 840 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 840 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2604 2662 2836 3297 4503 4588 5659 2043
Size: 561 58 174 461 1206 85 1071 2826
Fragments arranged by size:
2826 1206 1071 561 461 174 85 58
BsmI GAATG_Cn'
Cuts at: 2014 2157 3953 4046 2014
Size: 143 1796 93 4410
Fragments arranged by size:
4410 1796 143 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3135 3135
Size: 6442
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2232 2280 2604 2662 2836 2981
Size: 399 253 189 48 324 58 174 145
Cuts at: 2981 3079 3264 3297 3419 3443 3761 4503 4588
Size: 98 185 33 122 24 318 742 85
Cuts at: 4588 5659 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 324 318 253 189
185 174 145 122 98 85 58 48
33 24
BspEI T'CCGG_A
Cuts at: 2382 6105 2382
Size: 3723 2719
BspHI T'CATG_A
Cuts at: 2529 4331 6061 2529
Size: 1802 1730 2910
Fragments arranged by size:
2910 1802 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3055 3797 844
Size: 2211 742 3489
Fragments arranged by size:
3489 2211 742
BsrBI CCG'CTC
Cuts at: 1394 1823 2721 4329 1394
Size: 429 898 1608 3507
Fragments arranged by size:
3507 1608 898 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4937 5111 65
Size: 1183 3689 174 1396
Fragments arranged by size:
3689 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2259 2805 5086 5273 1700
Size: 110 449 546 2281 187 2869
Fragments arranged by size:
2869 2281 546 449 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 4502 5514 4502
Size: 1012 5430
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6442
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 3403 1184
Size: 2219 4223
BstXI CCAn_nnnn'nTGG
Cuts at: 2533 2533
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 2541 3279 3772 4139 4524 4541 5993 6079
Size: 569 738 493 367 385 17 1452 86
Cuts at: 6079 6091 6437 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 738 569 493 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2044 3192 3705 513
Size: 552 979 1148 513 3250
Fragments arranged by size:
3250 1148 979 552 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2715 487
Size: 683 56 1489 4214
Fragments arranged by size:
4214 1489 683 56
BtsI GCAGTG_nn'
Cuts at: 3958 4752 4772 3958
Size: 794 20 5628
Fragments arranged by size:
5628 794 20
ClaI AT'CG_AT
Cuts at: 1554 2595 1554
Size: 1041 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DraIII CAC_nnn'GTG
Cuts at: 2657 3358 2657
Size: 701 5741
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3688 5449 809
Size: 507 2372 1761 1802
Fragments arranged by size:
2372 1802 1761 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2410 2490
Size: 54 1425 50 275 18 292 288 80
Cuts at: 2490 3889 4778 6407 8
Size: 1399 889 1629 43
Fragments arranged by size:
1629 1425 1399 889 292 288 275 80
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3889 1537
Size: 275 2077 4090
Fragments arranged by size:
4090 2077 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 2139 2360 2877 2913 4372 1353
Size: 713 73 221 517 36 1459 3423
Fragments arranged by size:
3423 1459 713 517 221 73 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2153 2357 5029 5403 5549 387
Size: 1064 138 564 204 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 564 374 204 146 138
EcoICRI GAG'CTC
Cuts at: 2041 2602 2041
Size: 561 5881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2341 2588 2810 2937 3674 4505 5102
Size: 393 709 247 222 127 737 831 597
Cuts at: 5102 5889 6157 1239
Size: 787 268 1524
Fragments arranged by size:
1524 831 787 737 709 597 393 268
247 222 127
EcoNI CCTnn'n_nnAGG
Cuts at: 3527 3527
Size: 6442
EcoO109I rG'GnC_Cy
Cuts at: 2228 2420 2977 3067 3440 2228
Size: 192 557 90 373 5230
Fragments arranged by size:
5230 557 373 192 90
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
FspI TGC'GCA
Cuts at: 2184 4948 2184
Size: 2764 3678
FspAI rTGC'GCAy
Cuts at: 2184 2184
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 2509 2618 5589 1678
Size: 831 109 2971 2531
Fragments arranged by size:
2971 2531 831 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2155 2187 3362 3394 5083
Size: 32 135 32 583 32 1175 32 1689
Cuts at: 5083 5115 5157 5189 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1689 1175 583 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3584 3786 4032 669
Size: 988 281 1646 202 246 3079
Fragments arranged by size:
3079 1646 988 281 246 202
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2153 2220 2861 5556 5740 1305
Size: 848 67 641 2695 184 2007
Fragments arranged by size:
2695 2007 848 641 184 67
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2124 2492 10
Size: 54 1425 343 292 368 3960
Fragments arranged by size:
3960 1425 368 343 292 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 2162 2528 2531 3572 4400 4759
Size: 648 906 90 366 3 1041 828 359
Cuts at: 4759 4918 518
Size: 159 2042
Fragments arranged by size:
2042 1041 906 828 648 366 359 159
90 3
MspA1I CmG'CkG
Cuts at: 1606 3858 4535 5683 5928 1606
Size: 2252 677 1148 245 2120
Fragments arranged by size:
2252 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 2807 2807
Size: 6442
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5890
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NgoMIV G'CCGG_C
Cuts at: 2805 2805
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 2248 3094 3800 5345 1132
Size: 97 1019 846 706 1545 2229
Fragments arranged by size:
2229 1545 1019 846 706 97
PasI CC'CwG_GG
Cuts at: 1348 2974 1348
Size: 1626 4816
PciI A'CATG_T
Cuts at: 2244 5341 2244
Size: 3097 3345
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3358 1108
Size: 2250 4192
PfoI T'CCnGG_A
Cuts at: 2823 2823
Size: 6442
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PmlI CAC'GTG
Cuts at: 3274 3292 3274
Size: 18 6424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3067 3067
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PspOMI G'GGCC_C
Cuts at: 2228 2977 3439 2228
Size: 749 462 5231
Fragments arranged by size:
5231 749 462
PstI C_TGCA'G
Cuts at: 830 2275 3430 3794 830
Size: 1445 1155 364 3478
Fragments arranged by size:
3478 1445 1155 364
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
RsrII CG'GwC_CG
Cuts at: 3709 3709
Size: 6442
SacI G_AGCT'C
Cuts at: 2043 2604 2043
Size: 561 5881
SalI G'TCGA_C
Cuts at: 1936 3582 3784 1936
Size: 1646 202 4594
Fragments arranged by size:
4594 1646 202
SapI GCTCTTCn'nnn_
Cuts at: 2360 2360
Size: 6442
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 3174 4690 6402 3174
Size: 1516 1712 3214
Fragments arranged by size:
3214 1712 1516
SexAI A'CCwGG_T
Cuts at: 3120 3120
Size: 6442
SfcI C'TryA_G
Cuts at: 826 1045 2271 3420 3426 3790 4925 5606 5797
Size: 219 1226 1149 6 364 1135 681 191
Cuts at: 5797 826
Size: 1471
Fragments arranged by size:
1471 1226 1149 1135 681 364 219 191
6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2418 2418
Size: 6442
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 2226 2283 3769 1872
Size: 354 57 1486 4545
Fragments arranged by size:
4545 1486 354 57
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2094 2618 3524 4546 5447 5709
Size: 197 926 151 524 906 1022 901 262
Cuts at: 5709 820
Size: 1553
Fragments arranged by size:
1553 1022 926 906 901 524 262 197
151
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 3094 3800 1132
Size: 1962 706 3774
Fragments arranged by size:
3774 1962 706
SspI AAT'ATT
Cuts at: 5 52 4366 5
Size: 47 4314 2081
Fragments arranged by size:
4314 2081 47
StuI AGG'CCT
Cuts at: 1517 2375 3239 1517
Size: 858 864 4720
Fragments arranged by size:
4720 864 858
StyI C'CwwG_G
Cuts at: 513 1065 2850 3570 3881 513
Size: 552 1785 720 311 3074
Fragments arranged by size:
3074 1785 720 552 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2828 2855 3062 3447 3611 4469 4486
Size: 1255 1248 27 207 385 164 858 17
Cuts at: 4486 4639 4824 5269 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1248 858 445 385 207 185
164 153 27 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2951 3172 4688
Size: 274 80 33 51 1443 974 221 1516
Cuts at: 4688 6400 96
Size: 1712 138
Fragments arranged by size:
1712 1516 1443 974 274 221 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3281 3313 3686 3718 6402 6434 3281
Size: 32 373 32 2684 32 3289
Fragments arranged by size:
3289 2684 373 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3152 3641 1316
Size: 1836 489 4117
Fragments arranged by size:
4117 1836 489
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3190 3190
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 2224 2281 3767 1870
Size: 354 57 1486 4545
Fragments arranged by size:
4545 1486 354 57
XmnI GAAnn'nnTTC
Cuts at: 3174 4571 3174
Size: 1397 5045
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbeI BbvCI BfrBI BsiWI
BstZ17I Bsu36I EcoRV FalI FseI KasI NarI NruI
NsiI PacI PspXI PsrI PvuII SacII SanDI SfoI
SrfI SwaI