Restriction map for
pFN21AE2003
(Circular) (Six-base) MAPSORT of: pFN21AE2003.seq Check: 5217 from: 1 to: 6439
pFN21AE2003 6439 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2200 3438 4249 278
Size: 53 83 186 936 664 1238 811 2468
Fragments arranged by size:
2468 1238 936 811 664 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2931 3092 3782 1937
Size: 994 161 690 4594
Fragments arranged by size:
4594 994 690 161
Acc65I G'GTAC_C
Cuts at: 3760 3760
Size: 6439
AclI AA'CG_TT
Cuts at: 1563 4566 4939 1563
Size: 3003 373 3063
Fragments arranged by size:
3063 3003 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2188 2423 3090 3349 3661 4502 5886 6154
Size: 556 235 667 259 312 841 1384 268
Cuts at: 6154 1632
Size: 1917
Fragments arranged by size:
1917 1384 841 667 556 312 268 259
235
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6242
AflIII A'CryG_T
Cuts at: 1203 5338 6315 1203
Size: 4135 977 1327
Fragments arranged by size:
4135 1327 977
AgeI A'CCGG_T
Cuts at: 5270 5270
Size: 6439
AhdI GACnn_n'nnGTC
Cuts at: 5168 5168
Size: 6439
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6439
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3015 3047 3015
Size: 32 6407
AlwNI CAG_nnn'CTG
Cuts at: 4182 5754 4182
Size: 1572 4867
ApaLI G'TGCA_C
Cuts at: 4496 5652 4496
Size: 1156 5283
ApoI r'AATT_y
Cuts at: 1493 2249 2715 3748 3974 4126 6235 1493
Size: 756 466 1033 226 152 2109 1697
Fragments arranged by size:
2109 1697 1033 756 466 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4993 160
Size: 872 3961 1606
Fragments arranged by size:
3961 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 3764 1870
Size: 73 69 1752 4545
Fragments arranged by size:
4545 1752 73 69
BamHI G'GATC_C
Cuts at: 3769 4136 3769
Size: 367 6072
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 3760 5215 618
Size: 325 217 627 1277 696 1455 1842
Fragments arranged by size:
1842 1455 1277 696 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 3396 3758 1391
Size: 622 1383 362 4072
Fragments arranged by size:
4072 1383 622 362
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2200 3501 928
Size: 971 301 1301 3866
Fragments arranged by size:
3866 1301 971 301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4630 4664 1903
Size: 34 2693 34 3678
Fragments arranged by size:
3678 2693 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4331 5541 4331
Size: 1210 5229
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6439
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5050 136
Size: 107 122 71 631 605 3378 1525
Fragments arranged by size:
3378 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3613 6434 3613
Size: 2821 3618
BlpI GC'TnA_GC
Cuts at: 3856 3856
Size: 6439
Bme1580I G_kGCm'C
Cuts at: 1790 4500 5656 1790
Size: 2710 1156 2573
Fragments arranged by size:
2710 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6439
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3702 4170 5128
Size: 416 53 83 399 24 2285 468 958
Cuts at: 5128 442
Size: 1753
Fragments arranged by size:
2285 1753 958 468 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6439
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3472 3472
Size: 6439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2355 2741 3395 3410 5099 1239
Size: 1116 386 654 15 1689 2579
Fragments arranged by size:
2579 1689 1116 654 386 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4564 5429 5727 4564
Size: 865 298 5276
Fragments arranged by size:
5276 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5102 882
Size: 617 3603 2219
Fragments arranged by size:
3603 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4230 493
Size: 711 3026 2702
Fragments arranged by size:
3026 2702 711
BsaBI GATnn'nnATC
Cuts at: 6433 6433
Size: 6439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2197 3435 4246 4628
Size: 53 83 186 936 664 1238 811 382
Cuts at: 4628 5263 275
Size: 635 1451
Fragments arranged by size:
1451 1238 936 811 664 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2982 4872 5270 5544 5691 6102 2982
Size: 1890 398 274 147 411 3319
Fragments arranged by size:
3319 1890 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2098 2128 1178
Size: 30 165 30 695 30 5489
Fragments arranged by size:
5489 695 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2423 3206 3239 1186
Size: 1237 783 33 4386
Fragments arranged by size:
4386 1237 783 33
BseYI C'CCAG_C
Cuts at: 3301 3326 5642 6138 3301
Size: 25 2316 496 3602
Fragments arranged by size:
3602 2316 496 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2149 1163
Size: 573 413 5453
Fragments arranged by size:
5453 573 413
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3889 4650 5678 1540
Size: 275 186 1888 761 1028 2301
Fragments arranged by size:
2301 1888 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2013 2796 4500 4585 5656 2013
Size: 783 1704 85 1071 2796
Fragments arranged by size:
2796 1704 1071 783 85
BsmI GAATG_Cn'
Cuts at: 3950 4043 3950
Size: 93 6346
BsmBI CGTCTCn'nnnn_
Cuts at: 2204 2204
Size: 6439
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2796 3396 3758 4500 4585 5656
Size: 399 223 783 600 362 742 85 1071
Cuts at: 5656 1391
Size: 2174
Fragments arranged by size:
2174 1071 783 742 600 399 362 223
85
BspEI T'CCGG_A
Cuts at: 2982 6102 2982
Size: 3120 3319
BspHI T'CATG_A
Cuts at: 4328 6058 4328
Size: 1730 4709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3794 844
Size: 2950 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3323 4326 1394
Size: 429 1500 1003 3507
Fragments arranged by size:
3507 1500 1003 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3740 4934 5108 65
Size: 1183 2492 1194 174 1396
Fragments arranged by size:
2492 1396 1194 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5083 5270 1700
Size: 110 3273 187 2869
Fragments arranged by size:
3273 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6439
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6439
BssSI C'ACGA_G
Cuts at: 4499 5511 4499
Size: 1012 5427
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6439
BstBI TT'CG_AA
Cuts at: 5243 5243
Size: 6439
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6439
BstYI r'GATC_y
Cuts at: 1972 3012 3471 3613 3769 4136 4521 4538 5990
Size: 1040 459 142 156 367 385 17 1452
Cuts at: 5990 6076 6088 6434 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1040 459 385 367 346 156
142 86 17 12
BstZ17I GTA'TAC
Cuts at: 2932 3093 2932
Size: 161 6278
BtgI C'CryG_G
Cuts at: 513 1065 2004 3699 513
Size: 552 939 1695 3253
Fragments arranged by size:
3253 1695 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5700
Fragments arranged by size:
5700 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 2137 2268 3955 4749 4769 2108
Size: 29 131 1687 794 20 3778
Fragments arranged by size:
3778 1687 794 131 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6439
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2056 2091 529
Size: 35 1492 35 4877
Fragments arranged by size:
4877 1492 35 35
DraI TTT'AAA
Cuts at: 3743 3743
Size: 6439
DraIII CAC_nnn'GTG
Cuts at: 3346 3346
Size: 6439
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3123 5446 809
Size: 507 1807 2323 1802
Fragments arranged by size:
2323 1807 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 3003 3886
Size: 54 1425 50 275 18 195 978 883
Cuts at: 3886 4775 6404 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 978 889 883 275 195 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3886 1537
Size: 275 2074 4090
Fragments arranged by size:
4090 2074 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2207 2672 3034 3676 3680 4369 1353
Size: 854 465 362 642 4 689 3423
Fragments arranged by size:
3423 854 689 642 465 362 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2607 5026 5400 5546 387
Size: 1064 138 1018 2419 374 146 1280
Fragments arranged by size:
2419 1280 1064 1018 374 146 138
EcoICRI GAG'CTC
Cuts at: 2011 2011
Size: 6439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2188 2355 2423 2741 3090 3349 3395
Size: 393 556 167 68 318 349 259 46
Cuts at: 3395 3410 3661 4502 5099 5886 6154 1239
Size: 15 251 841 597 787 268 1524
Fragments arranged by size:
1524 841 787 597 556 393 349 318
268 259 251 167 68 46 15
EcoRI G'AATT_C
Cuts at: 2249 2715 3748 4126 2249
Size: 466 1033 378 4562
Fragments arranged by size:
4562 1033 466 378
EcoRV GAT'ATC
Cuts at: 3445 3445
Size: 6439
FspI TGC'GCA
Cuts at: 4945 4945
Size: 6439
HaeII r_GCGC'y
Cuts at: 1678 5586 1678
Size: 3908 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2098 2130 2301 2333 3242
Size: 32 135 32 526 32 171 32 909
Cuts at: 3242 3274 5080 5112 5154 5186 1373
Size: 32 1806 32 42 32 2626
Fragments arranged by size:
2626 1806 909 526 171 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3783 4029 669
Size: 988 281 1845 246 3079
Fragments arranged by size:
3079 1845 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3600 748
Size: 2852 3587
HpaI GTT'AAC
Cuts at: 4029 4029
Size: 6439
KpnI G_GTAC'C
Cuts at: 3764 3764
Size: 6439
MfeI C'AATT_G
Cuts at: 4038 4038
Size: 6439
MluI A'CGCG_T
Cuts at: 6315 6315
Size: 6439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3025 5553 5737 1305
Size: 1720 2528 184 2007
Fragments arranged by size:
2528 2007 1720 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2027 3005 10
Size: 54 1425 343 195 978 3444
Fragments arranged by size:
3444 1425 978 343 195 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2801 4397 4756 4915 518
Size: 648 1635 1596 359 159 2042
Fragments arranged by size:
2042 1635 1596 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3855 4532 5680 5925 1606
Size: 2249 677 1148 245 2120
Fragments arranged by size:
2249 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3699 513
Size: 552 939 1695 3253
Fragments arranged by size:
3253 1695 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6439
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6439
NotI GC'GGCC_GC
Cuts at: 3886 3886
Size: 6439
NspI r_CATG'y
Cuts at: 1132 1229 3388 3797 5342 1132
Size: 97 2159 409 1545 2229
Fragments arranged by size:
2229 2159 1545 409 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6439
PciI A'CATG_T
Cuts at: 5338 5338
Size: 6439
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3537 1108
Size: 2429 4010
PfoI T'CCnGG_A
Cuts at: 3438 3438
Size: 6439
PmeI GTTT'AAAC
Cuts at: 3743 3743
Size: 6439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4508 4540 6021 6053 4508
Size: 32 1481 32 4894
Fragments arranged by size:
4894 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3701 1613
Size: 2088 4351
PsiI TTA'TAA
Cuts at: 4009 4009
Size: 6439
PstI C_TGCA'G
Cuts at: 830 2887 3269 3311 3791 830
Size: 2057 382 42 480 3478
Fragments arranged by size:
3478 2057 480 382 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6439
SacI G_AGCT'C
Cuts at: 2013 2013
Size: 6439
SalI G'TCGA_C
Cuts at: 1936 3781 1936
Size: 1845 4594
SbfI CC_TGCA'GG
Cuts at: 3791 3791
Size: 6439
ScaI AGT'ACT
Cuts at: 4687 6399 4687
Size: 1712 4727
SfcI C'TryA_G
Cuts at: 826 1045 2883 3265 3307 3667 3787 4922 5603
Size: 219 1838 382 42 360 120 1135 681
Cuts at: 5603 5794 826
Size: 191 1471
Fragments arranged by size:
1838 1471 1135 681 382 360 219 191
120 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6439
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6439
SmaI CCC'GGG
Cuts at: 1872 3766 1872
Size: 1894 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2012 4543 5444 5706 820
Size: 197 926 69 2531 901 262 1553
Fragments arranged by size:
2531 1553 926 901 262 197 69
SnaBI TAC'GTA
Cuts at: 493 4230 493
Size: 3737 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6439
SphI G_CATG'C
Cuts at: 1132 3797 1132
Size: 2665 3774
SspI AAT'ATT
Cuts at: 5 52 2956 4363 5
Size: 47 2904 1407 2081
Fragments arranged by size:
2904 2081 1407 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6439
StyI C'CwwG_G
Cuts at: 513 1065 2004 3699 3878 513
Size: 552 939 1695 179 3074
Fragments arranged by size:
3074 1695 939 552 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2310 3552 4466 4483 4636 4821 5266
Size: 1255 730 1242 914 17 153 185 445
Cuts at: 5266 325
Size: 1498
Fragments arranged by size:
1498 1255 1242 914 730 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2441 4685 6397
Size: 274 80 33 51 1443 464 2244 1712
Cuts at: 6397 96
Size: 138
Fragments arranged by size:
2244 1712 1443 464 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3271 3303 6399 6431 3271
Size: 32 3096 32 3279
Fragments arranged by size:
3279 3096 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6439
XbaI T'CTAG_A
Cuts at: 3616 3775 3616
Size: 159 6280
XhoI C'TCGA_G
Cuts at: 1943 2012 1943
Size: 69 6370
XmaI C'CCGG_G
Cuts at: 1870 3764 1870
Size: 1894 4545
XmnI GAAnn'nnTTC
Cuts at: 3141 4568 3141
Size: 1427 5012
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2198 3436 4247 276
Size: 53 83 186 936 664 1238 811 2468
Fragments arranged by size:
2468 1238 936 811 664 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI Bsu36I EcoNI EcoO109I FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI