Restriction map for
pFN21AE1997
(Circular) (Six-base) MAPSORT of: pFN21AE1997.seq Check: 6401 from: 1 to: 7141
pFN21AE1997 7141 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2065 2065
Size: 7141
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4283 4951 278
Size: 53 83 186 936 2747 668 2468
Fragments arranged by size:
2747 2468 936 668 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3146 4484 1937
Size: 1209 1338 4594
Fragments arranged by size:
4594 1338 1209
Acc65I G'GTAC_C
Cuts at: 4462 4462
Size: 7141
AclI AA'CG_TT
Cuts at: 1563 5268 5641 1563
Size: 3705 373 3063
Fragments arranged by size:
3705 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4371 5204 6588 6856 1632
Size: 2739 833 1384 268 1917
Fragments arranged by size:
2739 1917 1384 833 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6944
AflIII A'CryG_T
Cuts at: 1203 6040 7017 1203
Size: 4837 977 1327
Fragments arranged by size:
4837 1327 977
AgeI A'CCGG_T
Cuts at: 5972 5972
Size: 7141
AhdI GACnn_n'nnGTC
Cuts at: 5870 5870
Size: 7141
AleI CACnn'nnGTG
Cuts at: 1166 2324 1166
Size: 1158 5983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7141
AlwNI CAG_nnn'CTG
Cuts at: 2626 3713 4884 6456 2626
Size: 1087 1171 1572 3311
Fragments arranged by size:
3311 1572 1171 1087
ApaI G_GGCC'C
Cuts at: 2088 2088
Size: 7141
ApaLI G'TGCA_C
Cuts at: 5198 6354 5198
Size: 1156 5985
ApoI r'AATT_y
Cuts at: 1493 2542 3804 4450 4676 4828 6937 1493
Size: 1049 1262 646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1262 1049 646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3252 5695 160
Size: 872 2220 2443 1606
Fragments arranged by size:
2443 2220 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
AvaI C'yCGr_G
Cuts at: 1870 1943 4466 1870
Size: 73 2523 4545
Fragments arranged by size:
4545 2523 73
BamHI G'GATC_C
Cuts at: 2265 4471 4838 2265
Size: 2206 367 4568
Fragments arranged by size:
4568 2206 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2038 2093 2135 3320 4271
Size: 325 217 627 251 55 42 1185 951
Cuts at: 4271 4462 5917 618
Size: 191 1455 1842
Fragments arranged by size:
1842 1455 1185 951 627 325 251 217
191 55 42
BanII G_rGCy'C
Cuts at: 1391 2088 3223 4460 1391
Size: 697 1135 1237 4072
Fragments arranged by size:
4072 1237 1135 697
BbeI G_GCGC'C
Cuts at: 2042 2097 2042
Size: 55 7086
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2420 3412 3542 4344 928
Size: 971 521 992 130 802 3725
Fragments arranged by size:
3725 992 971 802 521 130
BbvCI CC'TCA_GC
Cuts at: 2600 2600
Size: 7141
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2041 2075 5332 5366 1903
Size: 34 104 34 3257 34 3678
Fragments arranged by size:
3678 3257 104 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5033 6243 5033
Size: 1210 5931
BclI T'GATC_A
Cuts at: 1548 3526 1548
Size: 1978 5163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2282 4378 5752
Size: 107 122 71 631 605 610 2096 1374
Cuts at: 5752 136
Size: 1525
Fragments arranged by size:
2096 1525 1374 631 610 605 122 107
71
BglII A'GATC_T
Cuts at: 7136 7136
Size: 7141
BlpI GC'TnA_GC
Cuts at: 3101 4558 3101
Size: 1457 5684
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2138 4274 5202 6358 1790
Size: 298 50 2136 928 1156 2573
Fragments arranged by size:
2573 2136 1156 928 298 50
BmgBI CAC'GTC
Cuts at: 1318 2695 3786 1318
Size: 1377 1091 4673
Fragments arranged by size:
4673 1377 1091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2024 3184 3723
Size: 416 53 83 399 24 607 1160 539
Cuts at: 3723 4171 4872 5830 442
Size: 448 701 958 1753
Fragments arranged by size:
1753 1160 958 701 607 539 448 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2044 2603 4408 5801 1239
Size: 805 559 1805 1393 2579
Fragments arranged by size:
2579 1805 1393 805 559
Bpu10I CC'TnA_GC
Cuts at: 2600 2600
Size: 7141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5266 6131 6429 5266
Size: 865 298 5978
Fragments arranged by size:
5978 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 2798 5804 882
Size: 617 600 699 3006 2219
Fragments arranged by size:
3006 2219 699 617 600
BsaAI yAC'GTr
Cuts at: 493 1204 2236 3043 4932 493
Size: 711 1032 807 1889 2702
Fragments arranged by size:
2702 1889 1032 807 711
BsaBI GATnn'nnATC
Cuts at: 2870 3128 3612 4006 7135 2870
Size: 258 484 394 3129 2876
Fragments arranged by size:
3129 2876 484 394 258
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2039 2094 4280 4948
Size: 53 83 186 936 506 55 2186 668
Cuts at: 4948 5330 5965 275
Size: 382 635 1451
Fragments arranged by size:
2186 1451 936 668 635 506 382 186
83 55 53
BsaWI w'CCGG_w
Cuts at: 3261 5574 5972 6246 6393 6804 3261
Size: 2313 398 274 147 411 3598
Fragments arranged by size:
3598 2313 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6916
Fragments arranged by size:
6916 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 2210 2429 4066 1186
Size: 855 169 219 1637 4261
Fragments arranged by size:
4261 1637 855 219 169
BseYI C'CCAG_C
Cuts at: 4235 6344 6840 4235
Size: 2109 496 4536
Fragments arranged by size:
4536 2109 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 1163
Size: 573 522 6046
Fragments arranged by size:
6046 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4591 5352 6380 1540
Size: 275 186 2590 761 1028 2301
Fragments arranged by size:
2590 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4119 5202 5287 6358 4119
Size: 1083 85 1071 4902
Fragments arranged by size:
4902 1083 1071 85
BsmI GAATG_Cn'
Cuts at: 2591 4652 4745 2591
Size: 2061 93 4987
Fragments arranged by size:
4987 2061 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2138 3223 4119 4274 4460 5202
Size: 399 298 50 1085 896 155 186 742
Cuts at: 5202 5287 6358 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1085 1071 896 742 399 298 186
155 85 50
BspEI T'CCGG_A
Cuts at: 3261 6804 3261
Size: 3543 3598
BspHI T'CATG_A
Cuts at: 3090 5030 6760 3090
Size: 1940 1730 3471
Fragments arranged by size:
3471 1940 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2065 2634 4496 844
Size: 1221 569 1862 3489
Fragments arranged by size:
3489 1862 1221 569
BsrBI CCG'CTC
Cuts at: 1394 1823 5028 1394
Size: 429 3205 3507
Fragments arranged by size:
3507 3205 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2415 3030 3297 5636 5810 65
Size: 1183 1167 615 267 2339 174 1396
Fragments arranged by size:
2339 1396 1183 1167 615 267 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2202 2462 3921 5785 5972 1700
Size: 110 392 260 1459 1864 187 2869
Fragments arranged by size:
2869 1864 1459 392 260 187 110
BsrGI T'GTAC_A
Cuts at: 96 2763 2952 96
Size: 2667 189 4285
Fragments arranged by size:
4285 2667 189
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7141
BssSI C'ACGA_G
Cuts at: 3164 5201 6213 3164
Size: 2037 1012 4092
Fragments arranged by size:
4092 2037 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3014 794
Size: 2220 4921
BstBI TT'CG_AA
Cuts at: 5945 5945
Size: 7141
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7141
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 3703 2455
Size: 1248 5893
BstYI r'GATC_y
Cuts at: 1972 2241 2265 4471 4838 5223 5240 6692 6778
Size: 269 24 2206 367 385 17 1452 86
Cuts at: 6778 6790 7136 1972
Size: 12 346 1977
Fragments arranged by size:
2206 1977 1452 385 367 346 269 86
24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2088 2130 3150 4376 513
Size: 552 939 84 42 1020 1226 3278
Fragments arranged by size:
3278 1226 1020 939 552 84 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6402
Fragments arranged by size:
6402 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 3012 3112 4657 5451 5471 2042
Size: 970 100 1545 794 20 3712
Fragments arranged by size:
3712 1545 970 794 100 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7141
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7106
DraI TTT'AAA
Cuts at: 4445 4445
Size: 7141
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3567 6148 809
Size: 507 2251 2581 1802
Fragments arranged by size:
2581 2251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2283 4222 4588
Size: 54 1425 50 275 18 453 1939 366
Cuts at: 4588 5477 7106 8
Size: 889 1629 43
Fragments arranged by size:
1939 1629 1425 889 453 366 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4588 1537
Size: 275 2776 4090
Fragments arranged by size:
4090 2776 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5071 1353
Size: 3718 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2117 5728 6102 6248 387
Size: 1064 138 528 3611 374 146 1280
Fragments arranged by size:
3611 1280 1064 528 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2044 2603 4371 4408 5204 5801 6588
Size: 393 412 559 1768 37 796 597 787
Cuts at: 6588 6856 1239
Size: 268 1524
Fragments arranged by size:
1768 1524 796 787 597 559 412 393
268 37
EcoNI CCTnn'n_nnAGG
Cuts at: 3242 3242
Size: 7141
EcoO109I rG'GnC_Cy
Cuts at: 2084 2084
Size: 7141
EcoRI G'AATT_C
Cuts at: 2542 4450 4828 2542
Size: 1908 378 4855
Fragments arranged by size:
4855 1908 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 7141
FspI TGC'GCA
Cuts at: 5647 5647
Size: 7141
HaeII r_GCGC'y
Cuts at: 1678 2042 2097 4144 6288 1678
Size: 364 55 2047 2144 2531
Fragments arranged by size:
2531 2144 2047 364 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 3309 3341 5782
Size: 32 135 32 809 32 896 32 2441
Cuts at: 5782 5814 5856 5888 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2441 896 809 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4485 4731 669
Size: 988 281 2547 246 3079
Fragments arranged by size:
3079 2547 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3623 3677 4028 748
Size: 2875 54 351 3861
Fragments arranged by size:
3861 2875 351 54
HpaI GTT'AAC
Cuts at: 4731 4731
Size: 7141
KasI G'GCGC_C
Cuts at: 2038 2093 2038
Size: 55 7086
KpnI G_GTAC'C
Cuts at: 4466 4466
Size: 7141
MfeI C'AATT_G
Cuts at: 4740 4740
Size: 7141
MluI A'CGCG_T
Cuts at: 7017 7017
Size: 7141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2335 6255 6439 1305
Size: 1030 3920 184 2007
Fragments arranged by size:
3920 2007 1030 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2285 10
Size: 54 1425 343 453 4866
Fragments arranged by size:
4866 1425 453 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 2558 2697 2813 3095 3134 3140
Size: 648 1158 234 139 116 282 39 6
Cuts at: 3140 3701 5099 5458 5617 518
Size: 561 1398 359 159 2042
Fragments arranged by size:
2042 1398 1158 648 561 359 282 234
159 139 116 39 6
MspA1I CmG'CkG
Cuts at: 1606 2651 2978 4557 5234 6382 6627 1606
Size: 1045 327 1579 677 1148 245 2120
Fragments arranged by size:
2120 1579 1148 1045 677 327 245
NaeI GCC'GGC
Cuts at: 2204 2464 3923 2204
Size: 260 1459 5422
Fragments arranged by size:
5422 1459 260
NarI GG'CG_CC
Cuts at: 2039 2094 2039
Size: 55 7086
NcoI C'CATG_G
Cuts at: 513 1065 2004 2130 513
Size: 552 939 126 5524
Fragments arranged by size:
5524 939 552 126
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7141
NgoMIV G'CCGG_C
Cuts at: 2202 2462 3921 2202
Size: 260 1459 5422
Fragments arranged by size:
5422 1459 260
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7141
NotI GC'GGCC_GC
Cuts at: 4588 4588
Size: 7141
NspI r_CATG'y
Cuts at: 1132 1229 3695 4499 6044 1132
Size: 97 2466 804 1545 2229
Fragments arranged by size:
2466 2229 1545 804 97
PasI CC'CwG_GG
Cuts at: 1348 3223 1348
Size: 1875 5266
PciI A'CATG_T
Cuts at: 6040 6040
Size: 7141
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7141
PfoI T'CCnGG_A
Cuts at: 2176 2176
Size: 7141
PmeI GTTT'AAAC
Cuts at: 4445 4445
Size: 7141
PmlI CAC'GTG
Cuts at: 2236 2236
Size: 7141
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5210 5242 6723 6755 5210
Size: 32 1481 32 5596
Fragments arranged by size:
5596 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3245 1613
Size: 1632 5509
PsiI TTA'TAA
Cuts at: 2348 4711 2348
Size: 2363 4778
PspOMI G'GGCC_C
Cuts at: 2084 2084
Size: 7141
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2756 2788 2756
Size: 32 7109
PstI C_TGCA'G
Cuts at: 830 2076 4493 830
Size: 1246 2417 3478
Fragments arranged by size:
3478 2417 1246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7141
PvuII CAG'CTG
Cuts at: 2651 2978 2651
Size: 327 6814
SalI G'TCGA_C
Cuts at: 1936 4483 1936
Size: 2547 4594
SbfI CC_TGCA'GG
Cuts at: 2076 4493 2076
Size: 2417 4724
ScaI AGT'ACT
Cuts at: 3086 3727 5389 7101 3086
Size: 641 1662 1712 3126
Fragments arranged by size:
3126 1712 1662 641
SfcI C'TryA_G
Cuts at: 826 1045 2072 4292 4489 5624 6305 6496 826
Size: 219 1027 2220 197 1135 681 191 1471
Fragments arranged by size:
2220 1471 1135 1027 681 219 197 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2282 2282
Size: 7141
SfoI GGC'GCC
Cuts at: 2040 2095 2040
Size: 55 7086
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7141
SgrAI Cr'CCGG_yG
Cuts at: 1700 3921 1700
Size: 2221 4920
SmaI CCC'GGG
Cuts at: 1872 4468 1872
Size: 2596 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5245 6146 6408 820
Size: 197 926 3302 901 262 1553
Fragments arranged by size:
3302 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3043 4932 493
Size: 2550 1889 2702
Fragments arranged by size:
2702 2550 1889
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7141
SphI G_CATG'C
Cuts at: 1132 4499 1132
Size: 3367 3774
SspI AAT'ATT
Cuts at: 5 52 5065 5
Size: 47 5013 2081
Fragments arranged by size:
5013 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7141
StyI C'CwwG_G
Cuts at: 513 1065 2004 2130 3903 4580 513
Size: 552 939 126 1773 677 3074
Fragments arranged by size:
3074 1773 939 677 552 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2461 2927 3128 3215 4289 5168 5185
Size: 1255 881 466 201 87 1074 879 17
Cuts at: 5185 5338 5523 5968 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1074 881 879 466 445 201
185 153 87 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2763 2952 3084
Size: 274 80 33 51 1443 786 189 132
Cuts at: 3084 3725 5387 7099 96
Size: 641 1662 1712 138
Fragments arranged by size:
1712 1662 1443 786 641 274 189 138
132 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3968 4000 7101 7133 3968
Size: 32 3101 32 3976
Fragments arranged by size:
3976 3101 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3870 1316
Size: 2554 4587
XbaI T'CTAG_A
Cuts at: 4477 4477
Size: 7141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2019 4383 2011
Size: 8 2364 4769
Fragments arranged by size:
4769 2364 8
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7141
XmaI C'CCGG_G
Cuts at: 1870 4466 1870
Size: 2596 4545
XmnI GAAnn'nnTTC
Cuts at: 2985 5270 2985
Size: 2285 4856
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4281 4949 276
Size: 53 83 186 936 2747 668 2468
Fragments arranged by size:
2747 2468 936 668 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NruI NsiI PacI PpuMI PspXI RsrII SacI SacII
SanDI SapI SexAI SrfI SwaI