Restriction map for
pFN21AE1996
(Circular) (Six-base) MAPSORT of: pFN21AE1996.seq Check: 2506 from: 1 to: 6430
pFN21AE1996 6430 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2858 2858
Size: 6430
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2057 2777 3372 3398 4493 5877 6145 1632
Size: 425 720 595 26 1095 1384 268 1917
Fragments arranged by size:
1917 1384 1095 720 595 425 268 26
AfeI AGC'GCT
Cuts at: 3383 3383
Size: 6430
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 3699 5329 6306 1203
Size: 2496 1630 977 1327
Fragments arranged by size:
2496 1630 1327 977
AgeI A'CCGG_T
Cuts at: 2826 5261 2826
Size: 2435 3995
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2039 3116 1184
Size: 855 1077 4498
Fragments arranged by size:
4498 1077 855
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 3566 3598 2042
Size: 32 1492 32 4874
Fragments arranged by size:
4874 1492 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3328 3376 3616 4173 5745 3328
Size: 48 240 557 1572 4013
Fragments arranged by size:
4013 1572 557 240 48
ApaI G_GGCC'C
Cuts at: 2246 2246
Size: 6430
ApaLI G'TGCA_C
Cuts at: 4487 5643 4487
Size: 1156 5274
ApoI r'AATT_y
Cuts at: 1493 2930 3739 3965 4117 6226 1493
Size: 1437 809 226 152 2109 1697
Fragments arranged by size:
2109 1697 1437 809 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2129 2149 2168 2224 2234 2740 3242
Size: 73 186 20 19 56 10 506 502
Cuts at: 3242 3494 3755 1870
Size: 252 261 4545
Fragments arranged by size:
4545 506 502 261 252 186 73 56
20 19 10
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 2261 2300 2558 3221
Size: 325 217 627 338 136 39 258 663
Cuts at: 3221 3661 3677 3751 5206 618
Size: 440 16 74 1455 1842
Fragments arranged by size:
1842 1455 663 627 440 338 325 258
217 136 74 39 16
BanII G_rGCy'C
Cuts at: 1391 2217 2246 2582 2675 2873 3243 3501 3570
Size: 826 29 336 93 198 370 258 69
Cuts at: 3570 3597 3749 1391
Size: 27 152 4072
Fragments arranged by size:
4072 826 370 336 258 198 152 93
69 29 27
BbeI G_GCGC'C
Cuts at: 2129 2265 2562 3225 3665 3681 2129
Size: 136 297 663 440 16 4878
Fragments arranged by size:
4878 663 440 297 136 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3165 3394 928
Size: 971 1266 229 3964
Fragments arranged by size:
3964 1266 971 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3271 3305 4621 4655 1903
Size: 34 50 34 1250 34 1316 34 3678
Fragments arranged by size:
3678 1316 1250 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2212 3293 5041
Size: 107 122 71 631 605 540 1081 1748
Cuts at: 5041 136
Size: 1525
Fragments arranged by size:
1748 1525 1081 631 605 540 122 107
71
BglII A'GATC_T
Cuts at: 3459 6425 3459
Size: 2966 3464
BlpI GC'TnA_GC
Cuts at: 2072 3596 3847 2072
Size: 1524 251 4655
Fragments arranged by size:
4655 1524 251
Bme1580I G_kGCm'C
Cuts at: 1790 2246 4491 5647 1790
Size: 456 2245 1156 2573
Fragments arranged by size:
2573 2245 1156 456
BmgBI CAC'GTC
Cuts at: 1318 2725 1318
Size: 1407 5023
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3517 4161 5119
Size: 416 53 83 399 24 2100 644 958
Cuts at: 5119 442
Size: 1753
Fragments arranged by size:
2100 1753 958 644 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2794 3478 3682 2794
Size: 684 204 5542
Fragments arranged by size:
5542 684 204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2254 2609 2918 3740 5090 1239
Size: 1015 355 309 822 1350 2579
Fragments arranged by size:
2579 1350 1015 822 355 309
Bpu10I CC'TnA_GC
Cuts at: 3470 3470
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4555 5420 5718 4555
Size: 865 298 5267
Fragments arranged by size:
5267 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3548 5093 882
Size: 617 2049 1545 2219
Fragments arranged by size:
2219 2049 1545 617
BsaAI yAC'GTr
Cuts at: 493 1204 4221 493
Size: 711 3017 2702
Fragments arranged by size:
3017 2702 711
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2126 2262 2404 2559
Size: 53 83 186 936 593 136 142 155
Cuts at: 2559 2765 3222 3662 3678 4237 4619 5254 275
Size: 206 457 440 16 559 382 635 1451
Fragments arranged by size:
1451 936 635 593 559 457 440 382
206 186 155 142 136 83 53 16
BsaWI w'CCGG_w
Cuts at: 2826 4863 5261 5535 5682 6093 2826
Size: 2037 398 274 147 411 3163
Fragments arranged by size:
3163 2037 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3566 3596 1178
Size: 30 165 30 2163 30 4012
Fragments arranged by size:
4012 2163 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2881 2915 2918 3272 3542 3566 1186
Size: 1695 34 3 354 270 24 4050
Fragments arranged by size:
4050 1695 354 270 34 24 3
BseYI C'CCAG_C
Cuts at: 2207 3673 5633 6129 2207
Size: 1466 1960 496 2508
Fragments arranged by size:
2508 1960 1466 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2714 3122 1163
Size: 573 978 408 4471
Fragments arranged by size:
4471 978 573 408
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3525 3880 4641 5669 1540
Size: 275 186 1524 355 761 1028 2301
Fragments arranged by size:
2301 1524 1028 761 355 275 186
BsiHKAI G_wGCw'C
Cuts at: 2456 2873 3243 3501 3597 4491 4576 5647 2456
Size: 417 370 258 96 894 85 1071 3239
Fragments arranged by size:
3239 1071 894 417 370 258 96 85
BsiWI C'GTAC_G
Cuts at: 2940 2940
Size: 6430
BsmI GAATG_Cn'
Cuts at: 2376 3941 4034 2376
Size: 1565 93 4772
Fragments arranged by size:
4772 1565 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2246 2456 2582 2675 2873 3243
Size: 399 427 29 210 126 93 198 370
Cuts at: 3243 3501 3570 3597 3749 4491 4576 5647 1391
Size: 258 69 27 152 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 399 370 258 210
198 152 126 93 85 69 29 27
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 2667 4319 6049 2667
Size: 1652 1730 3048
Fragments arranged by size:
3048 1730 1652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2858 3006 3785 844
Size: 2014 148 779 3489
Fragments arranged by size:
3489 2014 779 148
BsrBI CCG'CTC
Cuts at: 1394 1823 2191 2548 4317 1394
Size: 429 368 357 1769 3507
Fragments arranged by size:
3507 1769 429 368 357
BsrDI GCAATG_nn'
Cuts at: 65 1248 4925 5099 65
Size: 1183 3677 174 1396
Fragments arranged by size:
3677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2029 2561 2826 5074 5261 1700
Size: 110 219 532 265 2248 187 2869
Fragments arranged by size:
2869 2248 532 265 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 2365 2474 3087 3443 4490 5502 2365
Size: 109 613 356 1047 1012 3293
Fragments arranged by size:
3293 1047 1012 613 356 109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 794
Size: 1301 5129
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6430
BstYI r'GATC_y
Cuts at: 1972 2309 2808 3066 3459 3760 4127 4512 4529
Size: 337 499 258 393 301 367 385 17
Cuts at: 4529 5981 6067 6079 6425 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 499 393 385 367 346 337
301 258 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2487 2793 3558 3603 3681 513
Size: 552 1422 306 765 45 78 3262
Fragments arranged by size:
3262 1422 765 552 306 78 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 3269 3946 4740 4760 3269
Size: 677 794 20 4939
Fragments arranged by size:
4939 794 677 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 2508 2646
Size: 54 1425 50 275 18 531 147 138
Cuts at: 2646 3285 3393 3522 3546 3877 4766 6395 8
Size: 639 108 129 24 331 889 1629 43
Fragments arranged by size:
1629 1425 889 639 531 331 275 147
138 129 108 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3522 3877 1537
Size: 275 1710 355 4090
Fragments arranged by size:
4090 1710 355 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 2555 4360 1353
Size: 987 215 1805 3423
Fragments arranged by size:
3423 1805 987 215
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2924 5017 5391 5537 387
Size: 1064 138 1335 2093 374 146 1280
Fragments arranged by size:
2093 1335 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2871 3241 3499 3595 2871
Size: 370 258 96 5706
Fragments arranged by size:
5706 370 258 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2254 2609 2777 2918 3372 3398
Size: 393 425 197 355 168 141 454 26
Cuts at: 3398 3740 4493 5090 5877 6145 1239
Size: 342 753 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 454 425 393 355
342 268 197 168 141 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2693 2759 2693
Size: 66 6364
EcoO109I rG'GnC_Cy
Cuts at: 2172 2243 3295 3373 3648 3715 2172
Size: 71 1052 78 275 67 4887
Fragments arranged by size:
4887 1052 275 78 71 67
EcoRI G'AATT_C
Cuts at: 2930 3739 4117 2930
Size: 809 378 5243
Fragments arranged by size:
5243 809 378
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2129 2265 2562 2894 3225 3385 3665 3681
Size: 451 136 297 332 331 160 280 16
Cuts at: 3681 5577 1678
Size: 1896 2531
Fragments arranged by size:
2531 1896 451 332 331 297 280 160
136 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5071 5103 5145 5177 1373
Size: 32 135 32 3499 32 42 32 2626
Fragments arranged by size:
3499 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2422 3774 4020 669
Size: 988 281 484 1352 246 3079
Fragments arranged by size:
3079 1352 988 484 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KasI G'GCGC_C
Cuts at: 2125 2261 2558 3221 3661 3677 2125
Size: 136 297 663 440 16 4878
Fragments arranged by size:
4878 663 440 297 136 16
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 3699 6306 3699
Size: 2607 3823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2405 5544 5728 1305
Size: 1100 3139 184 2007
Fragments arranged by size:
3139 2007 1100 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2510 3287 3395 10
Size: 54 1425 343 678 777 108 3045
Fragments arranged by size:
3045 1425 777 678 343 108 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3542 4388 4747 4906 518
Size: 648 2376 846 359 159 2042
Fragments arranged by size:
2376 2042 846 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2211 2806 2923 3198 3486 3637 3673
Size: 486 119 595 117 275 288 151 36
Cuts at: 3673 3727 3846 4523 5671 5916 1606
Size: 54 119 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 595 486 288 275 245
151 119 119 117 54 36
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 6430
NarI GG'CG_CC
Cuts at: 2126 2262 2559 3222 3662 3678 2126
Size: 136 297 663 440 16 4878
Fragments arranged by size:
4878 663 440 297 136 16
NcoI C'CATG_G
Cuts at: 513 1065 2487 513
Size: 552 1422 4456
Fragments arranged by size:
4456 1422 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 6430
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 3788 5333 1132
Size: 97 2559 1545 2229
Fragments arranged by size:
2559 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2257 3570 1348
Size: 909 1313 4208
Fragments arranged by size:
4208 1313 909
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3328 1108
Size: 2220 4210
PfoI T'CCnGG_A
Cuts at: 3306 3306
Size: 6430
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3176 3208 3566 3598 4499 4531 6012 6044 3176
Size: 32 358 32 901 32 1481 32 3562
Fragments arranged by size:
3562 1481 901 358 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3373 3715 3373
Size: 342 6088
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2242 2242
Size: 6430
PspXI vC'TCGA_Gb
Cuts at: 3242 3242
Size: 6430
PstI C_TGCA'G
Cuts at: 830 2028 2103 2883 3372 3782 830
Size: 1198 75 780 489 410 3478
Fragments arranged by size:
3478 1198 780 489 410 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2923 2923
Size: 6430
SacI G_AGCT'C
Cuts at: 2873 3243 3501 3597 2873
Size: 370 258 96 5706
Fragments arranged by size:
5706 370 258 96
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SapI GCTCTTCn'nnn_
Cuts at: 2340 2555 2340
Size: 215 6215
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 3188 3248 4678 6390 3188
Size: 60 1430 1712 3228
Fragments arranged by size:
3228 1712 1430 60
SexAI A'CCwGG_T
Cuts at: 2688 3333 2688
Size: 645 5785
SfcI C'TryA_G
Cuts at: 826 1045 2024 2099 2879 3368 3778 4913 5594
Size: 219 979 75 780 489 410 1135 681
Cuts at: 5594 5785 826
Size: 191 1471
Fragments arranged by size:
1471 1135 979 780 681 489 410 219
191 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 3293 3293
Size: 6430
SfoI GGC'GCC
Cuts at: 2127 2263 2560 3223 3663 3679 2127
Size: 136 297 663 440 16 4878
Fragments arranged by size:
4878 663 440 297 136 16
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 2561 1700
Size: 861 5569
SmaI CCC'GGG
Cuts at: 1872 2131 2151 2170 2226 2236 2742 3757 1872
Size: 259 20 19 56 10 506 1015 4545
Fragments arranged by size:
4545 1015 506 259 56 20 19 10
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3242 4534 5435 5697 820
Size: 197 926 1299 1292 901 262 1553
Fragments arranged by size:
1553 1299 1292 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SrfI GCCC'GGGC
Cuts at: 2131 2131
Size: 6430
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 2516 1517
Size: 999 5431
StyI C'CwwG_G
Cuts at: 513 1065 2487 2511 3869 513
Size: 552 1422 24 1358 3074
Fragments arranged by size:
3074 1422 1358 552 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2942 4457 4474 4627 4812 5257 325
Size: 1255 1362 1515 17 153 185 445 1498
Fragments arranged by size:
1515 1498 1362 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2064 3186 3246
Size: 274 80 33 51 1443 87 1122 60
Cuts at: 3246 4676 6388 96
Size: 1430 1712 138
Fragments arranged by size:
1712 1443 1430 1122 274 138 87 80
60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6390 6422 6390
Size: 32 6398
Tth111I GACn'n_nGTC
Cuts at: 1316 2417 2705 3134 3335 1316
Size: 1101 288 429 201 4411
Fragments arranged by size:
4411 1101 429 288 201
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2494 2494
Size: 6430
XhoI C'TCGA_G
Cuts at: 1943 3242 1943
Size: 1299 5131
XmaI C'CCGG_G
Cuts at: 1870 2129 2149 2168 2224 2234 2740 3755 1870
Size: 259 20 19 56 10 506 1015 4545
Fragments arranged by size:
4545 1015 506 259 56 20 19 10
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PsrI RsrII SacII SanDI SwaI