Restriction map for
pFN21AE1992
(Circular) (Six-base) MAPSORT of: pFN21AE1992.seq Check: 5920 from: 1 to: 6418
pFN21AE1992 6418 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4228 278
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3761 1937
Size: 1824 4594
Acc65I G'GTAC_C
Cuts at: 3288 3739 3288
Size: 451 5967
AclI AA'CG_TT
Cuts at: 1563 3219 4545 4918 1563
Size: 1656 1326 373 3063
Fragments arranged by size:
3063 1656 1326 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4481 5865 6133 1632
Size: 2849 1384 268 1917
Fragments arranged by size:
2849 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2483 820
Size: 197 1466 4755
Fragments arranged by size:
4755 1466 197
AflIII A'CryG_T
Cuts at: 1203 2070 3079 3081 3105 5317 6294 1203
Size: 867 1009 2 24 2212 977 1327
Fragments arranged by size:
2212 1327 1009 977 867 24 2
AgeI A'CCGG_T
Cuts at: 5249 5249
Size: 6418
AhdI GACnn_n'nnGTC
Cuts at: 5147 5147
Size: 6418
AleI CACnn'nnGTG
Cuts at: 1166 2420 1166
Size: 1254 5164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6418
AlwNI CAG_nnn'CTG
Cuts at: 2682 4161 5733 2682
Size: 1479 1572 3367
Fragments arranged by size:
3367 1572 1479
ApaLI G'TGCA_C
Cuts at: 4475 5631 4475
Size: 1156 5262
ApoI r'AATT_y
Cuts at: 1493 3184 3727 3953 4105 6214 1493
Size: 1691 543 226 152 2109 1697
Fragments arranged by size:
2109 1697 1691 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2772 4972 160
Size: 872 1740 2200 1606
Fragments arranged by size:
2200 1740 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2742 3112 3743 1870
Size: 73 134 665 370 631 4545
Fragments arranged by size:
4545 665 631 370 134 73
BamHI G'GATC_C
Cuts at: 3748 4115 3748
Size: 367 6051
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3288 3511 3739 5194
Size: 325 217 627 330 1171 223 228 1455
Cuts at: 5194 618
Size: 1842
Fragments arranged by size:
1842 1455 1171 627 330 325 228 223
217
BanII G_rGCy'C
Cuts at: 1391 2560 2712 3521 3737 1391
Size: 1169 152 809 216 4072
Fragments arranged by size:
4072 1169 809 216 152
BbeI G_GCGC'C
Cuts at: 2121 2121
Size: 6418
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5447
BbvCI CC'TCA_GC
Cuts at: 3596 3596
Size: 6418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4609 4643 1903
Size: 34 50 34 2588 34 3678
Fragments arranged by size:
3678 2588 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4310 5520 4310
Size: 1210 5208
BclI T'GATC_A
Cuts at: 1548 2427 1548
Size: 879 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2453 5029 136
Size: 107 122 71 631 605 781 2576 1525
Fragments arranged by size:
2576 1525 781 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3015 6413 3015
Size: 3398 3020
BlpI GC'TnA_GC
Cuts at: 3835 3835
Size: 6418
Bme1580I G_kGCm'C
Cuts at: 1790 3514 4479 5635 1790
Size: 1724 965 1156 2573
Fragments arranged by size:
2573 1724 1156 965
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6418
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2888 2975 4149
Size: 416 53 83 399 24 1471 87 1174
Cuts at: 4149 5107 442
Size: 958 1753
Fragments arranged by size:
1753 1471 1174 958 416 399 87 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6418
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2630 3604 2630
Size: 974 5444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2205 2726 3071 5078 1239
Size: 966 521 345 2007 2579
Fragments arranged by size:
2579 2007 966 521 345
Bpu10I CC'TnA_GC
Cuts at: 2865 3254 3596 3653 2865
Size: 389 342 57 5630
Fragments arranged by size:
5630 389 342 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4543 5408 5706 4543
Size: 865 298 5255
Fragments arranged by size:
5255 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2204 5081 882
Size: 617 705 2877 2219
Fragments arranged by size:
2877 2219 705 617
BsaAI yAC'GTr
Cuts at: 493 1204 3082 3604 4209 493
Size: 711 1878 522 605 2702
Fragments arranged by size:
2702 1878 711 605 522
BsaBI GATnn'nnATC
Cuts at: 6412 6412
Size: 6418
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2118 2861 4225 4607
Size: 53 83 186 936 585 743 1364 382
Cuts at: 4607 5242 275
Size: 635 1451
Fragments arranged by size:
1451 1364 936 743 635 585 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3087 4851 5249 5523 5670 6081 3087
Size: 1764 398 274 147 411 3424
Fragments arranged by size:
3424 1764 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2203 2233 1178
Size: 30 165 30 800 30 5363
Fragments arranged by size:
5363 800 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3015 1186
Size: 1829 4589
BseYI C'CCAG_C
Cuts at: 2053 3431 5621 6117 2053
Size: 1378 2190 496 2354
Fragments arranged by size:
2354 2190 1378 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3085 1163
Size: 573 1349 4496
Fragments arranged by size:
4496 1349 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3868 4629 5657 1540
Size: 275 186 1867 761 1028 2301
Fragments arranged by size:
2301 1867 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2700 4479 4564 5635 2700
Size: 1779 85 1071 3483
Fragments arranged by size:
3483 1779 1071 85
BsmI GAATG_Cn'
Cuts at: 3929 4022 3929
Size: 93 6325
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2560 2700 2712 3514 3521 3737 4479
Size: 399 770 140 12 802 7 216 742
Cuts at: 4479 4564 5635 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 802 770 742 399 216 140
85 12 7
BspEI T'CCGG_A
Cuts at: 3087 6081 3087
Size: 2994 3424
BspHI T'CATG_A
Cuts at: 4307 6037 4307
Size: 1730 4688
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3706 3717 3773 844
Size: 2862 11 56 3489
Fragments arranged by size:
3489 2862 56 11
BsrBI CCG'CTC
Cuts at: 1394 1823 2283 3645 4305 1394
Size: 429 460 1362 660 3507
Fragments arranged by size:
3507 1362 660 460 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2188 2545 4913 5087 65
Size: 1183 940 357 2368 174 1396
Fragments arranged by size:
2368 1396 1183 940 357 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2120 2410 5062 5249 1700
Size: 110 310 290 2652 187 2869
Fragments arranged by size:
2869 2652 310 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6418
BssSI C'ACGA_G
Cuts at: 4478 5490 4478
Size: 1012 5406
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3618 794
Size: 2824 3594
BstBI TT'CG_AA
Cuts at: 2341 5222 2341
Size: 2881 3537
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6418
BstXI CCAn_nnnn'nTGG
Cuts at: 2422 2422
Size: 6418
BstYI r'GATC_y
Cuts at: 1972 2404 3015 3748 4115 4500 4517 5969 6055
Size: 432 611 733 367 385 17 1452 86
Cuts at: 6055 6067 6413 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 733 611 432 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2547 2547
Size: 6418
BtgI C'CryG_G
Cuts at: 513 1065 2469 513
Size: 552 1404 4462
Fragments arranged by size:
4462 1404 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2044 487
Size: 683 56 818 4861
Fragments arranged by size:
4861 818 683 56
BtsI GCAGTG_nn'
Cuts at: 2399 2680 3041 3127 3207 3695 3934 4728 4748
Size: 281 361 86 80 488 239 794 20
Cuts at: 4748 2399
Size: 4069
Fragments arranged by size:
4069 794 488 361 281 239 86 80
20
ClaI AT'CG_AT
Cuts at: 1554 2763 1554
Size: 1209 5209
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6383
DraI TTT'AAA
Cuts at: 3722 3722
Size: 6418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5425 809
Size: 507 4109 1802
Fragments arranged by size:
4109 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2237 2412
Size: 54 1425 50 275 18 267 140 175
Cuts at: 2412 3636 3865 4754 6383 8
Size: 1224 229 889 1629 43
Fragments arranged by size:
1629 1425 1224 889 275 267 229 175
140 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3865 1537
Size: 275 2053 4090
Fragments arranged by size:
4090 2053 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 4348 1353
Size: 831 2164 3423
Fragments arranged by size:
3423 2164 831
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2744 5005 5379 5525 387
Size: 1064 138 1155 2261 374 146 1280
Fragments arranged by size:
2261 1280 1155 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2205 2726 3071 4481 5078 5865 6133
Size: 393 573 521 345 1410 597 787 268
Cuts at: 6133 1239
Size: 1524
Fragments arranged by size:
1524 1410 787 597 573 521 393 345
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3713 3713
Size: 6418
EcoO109I rG'GnC_Cy
Cuts at: 2293 2659 2877 3348 3649 2293
Size: 366 218 471 301 5062
Fragments arranged by size:
5062 471 366 301 218
EcoRI G'AATT_C
Cuts at: 3727 4105 3727
Size: 378 6040
FspI TGC'GCA
Cuts at: 4924 4924
Size: 6418
HaeII r_GCGC'y
Cuts at: 1678 2017 2121 5565 1678
Size: 339 104 3444 2531
Fragments arranged by size:
3444 2531 339 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2203 2235 5059 5091 5133
Size: 32 135 32 631 32 2824 32 42
Cuts at: 5133 5165 1373
Size: 32 2626
Fragments arranged by size:
2824 2626 631 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3762 4008 669
Size: 988 281 1824 246 3079
Fragments arranged by size:
3079 1824 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2256 2336 748
Size: 1508 80 4830
Fragments arranged by size:
4830 1508 80
HpaI GTT'AAC
Cuts at: 4008 4008
Size: 6418
KasI G'GCGC_C
Cuts at: 2117 2117
Size: 6418
KpnI G_GTAC'C
Cuts at: 3292 3743 3292
Size: 451 5967
MfeI C'AATT_G
Cuts at: 4017 4017
Size: 6418
MluI A'CGCG_T
Cuts at: 6294 6294
Size: 6418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2245 3388 5532 5716 1305
Size: 940 1143 2144 184 2007
Fragments arranged by size:
2144 2007 1143 940 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2239 10
Size: 54 1425 343 407 4189
Fragments arranged by size:
4189 1425 407 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2181 2420 2522 2774 2984 4376 4735
Size: 648 1015 239 102 252 210 1392 359
Cuts at: 4735 4894 518
Size: 159 2042
Fragments arranged by size:
2042 1392 1015 648 359 252 239 210
159 102
MspA1I CmG'CkG
Cuts at: 1606 2386 3270 3834 4511 5659 5904 1606
Size: 780 884 564 677 1148 245 2120
Fragments arranged by size:
2120 1148 884 780 677 564 245
NaeI GCC'GGC
Cuts at: 2122 2412 2122
Size: 290 6128
NarI GG'CG_CC
Cuts at: 2118 2118
Size: 6418
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5866
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6418
NgoMIV G'CCGG_C
Cuts at: 2120 2410 2120
Size: 290 6128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6418
NotI GC'GGCC_GC
Cuts at: 3865 3865
Size: 6418
NspI r_CATG'y
Cuts at: 1132 1229 2074 3776 5321 1132
Size: 97 845 1702 1545 2229
Fragments arranged by size:
2229 1702 1545 845 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6418
PciI A'CATG_T
Cuts at: 2070 5317 2070
Size: 3247 3171
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6418
PfoI T'CCnGG_A
Cuts at: 2670 2715 2670
Size: 45 6373
PmeI GTTT'AAAC
Cuts at: 3722 3722
Size: 6418
PmlI CAC'GTG
Cuts at: 3082 3604 3082
Size: 522 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3005 3037 4487 4519 6000 6032 3005
Size: 32 1450 32 1481 32 3391
Fragments arranged by size:
3391 1481 1450 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2659 2877 2293
Size: 366 218 5834
Fragments arranged by size:
5834 366 218
PshAI GACnn'nnGTC
Cuts at: 1613 2276 2379 2471 1613
Size: 663 103 92 5560
Fragments arranged by size:
5560 663 103 92
PsiI TTA'TAA
Cuts at: 3527 3988 3527
Size: 461 5957
PstI C_TGCA'G
Cuts at: 830 3537 3714 3770 830
Size: 2707 177 56 3478
Fragments arranged by size:
3478 2707 177 56
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6418
PvuII CAG'CTG
Cuts at: 3270 3270
Size: 6418
SalI G'TCGA_C
Cuts at: 1936 3760 1936
Size: 1824 4594
SbfI CC_TGCA'GG
Cuts at: 3770 3770
Size: 6418
ScaI AGT'ACT
Cuts at: 4666 6378 4666
Size: 1712 4706
SexAI A'CCwGG_T
Cuts at: 2319 2319
Size: 6418
SfcI C'TryA_G
Cuts at: 826 1045 2533 3533 3710 3766 4901 5582 5773
Size: 219 1488 1000 177 56 1135 681 191
Cuts at: 5773 826
Size: 1471
Fragments arranged by size:
1488 1471 1135 1000 681 219 191 177
56
SfoI GGC'GCC
Cuts at: 2119 2119
Size: 6418
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6418
SmaI CCC'GGG
Cuts at: 1872 3114 3745 1872
Size: 1242 631 4545
Fragments arranged by size:
4545 1242 631
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 4522 5423 5685 820
Size: 197 926 540 2039 901 262 1553
Fragments arranged by size:
2039 1553 926 901 540 262 197
SnaBI TAC'GTA
Cuts at: 493 4209 493
Size: 3716 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6418
SphI G_CATG'C
Cuts at: 1132 3776 1132
Size: 2644 3774
SspI AAT'ATT
Cuts at: 5 52 3235 4342 5
Size: 47 3183 1107 2081
Fragments arranged by size:
3183 2081 1107 47
StuI AGG'CCT
Cuts at: 1517 2613 1517
Size: 1096 5322
StyI C'CwwG_G
Cuts at: 513 1065 2655 3857 513
Size: 552 1590 1202 3074
Fragments arranged by size:
3074 1590 1202 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2038 2770 4445 4462 4615 4800 5245
Size: 1255 458 732 1675 17 153 185 445
Cuts at: 5245 325
Size: 1498
Fragments arranged by size:
1675 1498 1255 732 458 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2073 4664 6376
Size: 274 80 33 51 1443 96 2591 1712
Cuts at: 6376 96
Size: 138
Fragments arranged by size:
2591 1712 1443 274 138 96 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6378 6410 6378
Size: 32 6386
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6418
XbaI T'CTAG_A
Cuts at: 3754 3754
Size: 6418
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6418
XmaI C'CCGG_G
Cuts at: 1870 3112 3743 1870
Size: 1242 631 4545
Fragments arranged by size:
4545 1242 631
XmnI GAAnn'nnTTC
Cuts at: 4547 4547
Size: 6418
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4226 276
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI XcmI