Restriction map for
pFN21AE1991
(Circular) (Six-base) MAPSORT of: pFN21AE1991.seq Check: 9083 from: 1 to: 6412
pFN21AE1991 6412 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4222 278
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3141 3338 3755 1937
Size: 1204 197 417 4594
Fragments arranged by size:
4594 1204 417 197
Acc65I G'GTAC_C
Cuts at: 3733 3733
Size: 6412
AclI AA'CG_TT
Cuts at: 1563 4539 4912 1563
Size: 2976 373 3063
Fragments arranged by size:
3063 2976 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 3076 3156 3555 4475 5859 6127 1632
Size: 654 790 80 399 920 1384 268 1917
Fragments arranged by size:
1917 1384 920 790 654 399 268 80
AflII C'TTAA_G
Cuts at: 820 1017 2987 820
Size: 197 1970 4245
Fragments arranged by size:
4245 1970 197
AflIII A'CryG_T
Cuts at: 1203 2511 2807 5311 6288 1203
Size: 1308 296 2504 977 1327
Fragments arranged by size:
2504 1327 1308 977 296
AgeI A'CCGG_T
Cuts at: 5243 5243
Size: 6412
AhdI GACnn_n'nnGTC
Cuts at: 5141 5141
Size: 6412
AleI CACnn'nnGTG
Cuts at: 1166 2248 1166
Size: 1082 5330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6412
AlwNI CAG_nnn'CTG
Cuts at: 4155 5727 4155
Size: 1572 4840
ApaI G_GGCC'C
Cuts at: 3357 3357
Size: 6412
ApaLI G'TGCA_C
Cuts at: 4469 5625 4469
Size: 1156 5256
ApoI r'AATT_y
Cuts at: 1493 2519 3047 3617 3629 3689 3721 3947 4099
Size: 1026 528 570 12 60 32 226 152
Cuts at: 4099 6208 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1026 570 528 226 152 60
32 12
AseI AT'TA_AT
Cuts at: 160 1032 2940 4966 160
Size: 872 1908 2026 1606
Fragments arranged by size:
2026 1908 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
AvaI C'yCGr_G
Cuts at: 1870 1943 2828 3737 1870
Size: 73 885 909 4545
Fragments arranged by size:
4545 909 885 73
BamHI G'GATC_C
Cuts at: 3742 4109 3742
Size: 367 6045
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3733 5188 618
Size: 325 217 627 1946 1455 1842
Fragments arranged by size:
1946 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3357 3464 3731 1391
Size: 1966 107 267 4072
Fragments arranged by size:
4072 1966 267 107
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4603 4637 1903
Size: 34 2666 34 3678
Fragments arranged by size:
3678 2666 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2053 2348 2807 4304 5514 2053
Size: 295 459 1497 1210 2951
Fragments arranged by size:
2951 1497 1210 459 295
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5023 136
Size: 107 122 71 631 605 3351 1525
Fragments arranged by size:
3351 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6407 6407
Size: 6412
BlpI GC'TnA_GC
Cuts at: 2478 3829 2478
Size: 1351 5061
Bme1580I G_kGCm'C
Cuts at: 1790 3357 4473 5629 1790
Size: 1567 1116 1156 2573
Fragments arranged by size:
2573 1567 1156 1116
BmgBI CAC'GTC
Cuts at: 1318 2512 1318
Size: 1194 5218
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4143 5101 442
Size: 416 53 83 399 24 2726 958 1753
Fragments arranged by size:
2726 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3396 3440 5072 1239
Size: 2157 44 1632 2579
Fragments arranged by size:
2579 2157 1632 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3014 4537 5402 5700 3014
Size: 1523 865 298 3726
Fragments arranged by size:
3726 1523 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5075 882
Size: 617 3576 2219
Fragments arranged by size:
3576 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4203 493
Size: 711 2999 2702
Fragments arranged by size:
2999 2702 711
BsaBI GATnn'nnATC
Cuts at: 2715 3313 6406 2715
Size: 598 3093 2721
Fragments arranged by size:
3093 2721 598
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4219 4601 5236 275
Size: 53 83 186 936 2686 382 635 1451
Fragments arranged by size:
2686 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4845 5243 5517 5664 6075 4845
Size: 398 274 147 411 5182
Fragments arranged by size:
5182 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2865 2895 3225 3255 1178
Size: 30 165 30 1462 30 330 30 4335
Fragments arranged by size:
4335 1462 330 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2403 2628 1186
Size: 1217 225 4970
Fragments arranged by size:
4970 1217 225
BseYI C'CCAG_C
Cuts at: 5615 6111 5615
Size: 496 5916
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3130 1163
Size: 573 1394 4445
Fragments arranged by size:
4445 1394 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3862 4623 5651 1540
Size: 275 186 1861 761 1028 2301
Fragments arranged by size:
2301 1861 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4473 4558 5629 4473
Size: 85 1071 5256
Fragments arranged by size:
5256 1071 85
BsmI GAATG_Cn'
Cuts at: 3923 4016 3923
Size: 93 6319
BsmBI CGTCTCn'nnnn_
Cuts at: 2502 2502
Size: 6412
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3357 3464 3731 4473 4558 5629 1391
Size: 399 1567 107 267 742 85 1071 2174
Fragments arranged by size:
2174 1567 1071 742 399 267 107 85
BspEI T'CCGG_A
Cuts at: 6075 6075
Size: 6412
BspHI T'CATG_A
Cuts at: 4301 6031 4301
Size: 1730 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3767 844
Size: 2923 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4299 1394
Size: 429 2476 3507
Fragments arranged by size:
3507 2476 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2916 4907 5081 65
Size: 1183 1668 1991 174 1396
Fragments arranged by size:
1991 1668 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5056 5243 1700
Size: 110 3246 187 2869
Fragments arranged by size:
3246 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2332 96
Size: 2236 4176
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6412
BssSI C'ACGA_G
Cuts at: 4472 5484 4472
Size: 1012 5400
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2217 794
Size: 1423 4989
BstBI TT'CG_AA
Cuts at: 5216 5216
Size: 6412
BstEII G'GTnAC_C
Cuts at: 1184 2235 2586 1184
Size: 1051 351 5010
Fragments arranged by size:
5010 1051 351
BstYI r'GATC_y
Cuts at: 1972 3517 3742 4109 4494 4511 5963 6049 6061
Size: 1545 225 367 385 17 1452 86 12
Cuts at: 6061 6407 1972
Size: 346 1977
Fragments arranged by size:
1977 1545 1452 385 367 346 225 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2092 513
Size: 552 1027 4833
Fragments arranged by size:
4833 1027 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3659 487
Size: 683 56 2433 3240
Fragments arranged by size:
3240 2433 683 56
BtsI GCAGTG_nn'
Cuts at: 3928 4722 4742 3928
Size: 794 20 5598
Fragments arranged by size:
5598 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2564 2599 2773 2808 529
Size: 35 2000 35 174 35 4133
Fragments arranged by size:
4133 2000 174 35 35 35
DraI TTT'AAA
Cuts at: 3716 3716
Size: 6412
DraIII CAC_nnn'GTG
Cuts at: 2072 3329 2072
Size: 1257 5155
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5419 809
Size: 507 4103 1802
Fragments arranged by size:
4103 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3859 4748 6377
Size: 54 1425 50 275 18 2029 889 1629
Cuts at: 6377 8
Size: 43
Fragments arranged by size:
2029 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3859 1537
Size: 275 2047 4090
Fragments arranged by size:
4090 2047 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4342 1353
Size: 2989 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4999 5373 5519 387
Size: 1064 138 3410 374 146 1280
Fragments arranged by size:
3410 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 3076 3156 3396 3440 3555 4475
Size: 393 654 790 80 240 44 115 920
Cuts at: 4475 5072 5859 6127 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 920 790 787 654 597 393 268
240 115 80 44
EcoO109I rG'GnC_Cy
Cuts at: 3354 3354
Size: 6412
EcoRI G'AATT_C
Cuts at: 3617 3721 4099 3617
Size: 104 378 5930
Fragments arranged by size:
5930 378 104
EcoRV GAT'ATC
Cuts at: 3651 3651
Size: 6412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2084 2084
Size: 6412
FspI TGC'GCA
Cuts at: 4918 4918
Size: 6412
HaeII r_GCGC'y
Cuts at: 1678 5559 1678
Size: 3881 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2865 2897 3371 3403 5053
Size: 32 135 32 1293 32 474 32 1650
Cuts at: 5053 5085 5127 5159 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1650 1293 474 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3420 3756 4002 669
Size: 988 281 1482 336 246 3079
Fragments arranged by size:
3079 1482 988 336 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6412
HpaI GTT'AAC
Cuts at: 4002 4002
Size: 6412
KpnI G_GTAC'C
Cuts at: 3737 3737
Size: 6412
MfeI C'AATT_G
Cuts at: 4011 4011
Size: 6412
MluI A'CGCG_T
Cuts at: 6288 6288
Size: 6412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2485 2530 2624 5526 5710 1305
Size: 1180 45 94 2902 184 2007
Fragments arranged by size:
2902 2007 1180 184 94 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4590
Fragments arranged by size:
4590 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2248 3508 4370 4729 4888 518
Size: 648 1082 1260 862 359 159 2042
Fragments arranged by size:
2042 1260 1082 862 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3828 4505 5653 5898 1606
Size: 2222 677 1148 245 2120
Fragments arranged by size:
2222 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2092 513
Size: 552 1027 4833
Fragments arranged by size:
4833 1027 552
NdeI CA'TA_TG
Cuts at: 387 2420 387
Size: 2033 4379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6412
NotI GC'GGCC_GC
Cuts at: 3859 3859
Size: 6412
NspI r_CATG'y
Cuts at: 1132 1229 2220 2607 2811 3480 3770 5315 1132
Size: 97 991 387 204 669 290 1545 2229
Fragments arranged by size:
2229 1545 991 669 387 290 204 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6412
PciI A'CATG_T
Cuts at: 2807 5311 2807
Size: 2504 3908
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6412
PmeI GTTT'AAAC
Cuts at: 3716 3716
Size: 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4481 4513 5994 6026 4481
Size: 32 1481 32 4867
Fragments arranged by size:
4867 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6412
PsiI TTA'TAA
Cuts at: 2121 2543 2729 3982 2121
Size: 422 186 1253 4551
Fragments arranged by size:
4551 1253 422 186
PspOMI G'GGCC_C
Cuts at: 3353 3353
Size: 6412
PspXI vC'TCGA_Gb
Cuts at: 2828 2828
Size: 6412
PstI C_TGCA'G
Cuts at: 830 2531 3764 830
Size: 1701 1233 3478
Fragments arranged by size:
3478 1701 1233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6412
SalI G'TCGA_C
Cuts at: 1936 3754 1936
Size: 1818 4594
SbfI CC_TGCA'GG
Cuts at: 3764 3764
Size: 6412
ScaI AGT'ACT
Cuts at: 4660 6372 4660
Size: 1712 4700
SfcI C'TryA_G
Cuts at: 826 1045 2142 2527 3760 4895 5576 5767 826
Size: 219 1097 385 1233 1135 681 191 1471
Fragments arranged by size:
1471 1233 1135 1097 681 385 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6412
SmaI CCC'GGG
Cuts at: 1872 3739 1872
Size: 1867 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2828 2987 3029 4516 5417 5679
Size: 197 926 885 159 42 1487 901 262
Cuts at: 5679 820
Size: 1553
Fragments arranged by size:
1553 1487 926 901 885 262 197 159
42
SnaBI TAC'GTA
Cuts at: 493 4203 493
Size: 3710 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6412
SphI G_CATG'C
Cuts at: 1132 3770 1132
Size: 2638 3774
SspI AAT'ATT
Cuts at: 5 52 4336 5
Size: 47 4284 2081
Fragments arranged by size:
4284 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6412
StyI C'CwwG_G
Cuts at: 513 1065 2092 3851 513
Size: 552 1027 1759 3074
Fragments arranged by size:
3074 1759 1027 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2571 4439 4456 4609 4794 5239 325
Size: 1255 991 1868 17 153 185 445 1498
Fragments arranged by size:
1868 1498 1255 991 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2332 4658 6370
Size: 274 80 33 51 1443 355 2326 1712
Cuts at: 6370 96
Size: 138
Fragments arranged by size:
2326 1712 1443 355 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6372 6404 6372
Size: 32 6380
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6412
XbaI T'CTAG_A
Cuts at: 3062 3748 3062
Size: 686 5726
XhoI C'TCGA_G
Cuts at: 1943 2828 1943
Size: 885 5527
XmaI C'CCGG_G
Cuts at: 1870 3737 1870
Size: 1867 4545
XmnI GAAnn'nnTTC
Cuts at: 2263 4541 2263
Size: 2278 4134
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4220 276
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI