Restriction map for
pFN21AE1990
(Circular) (Six-base) MAPSORT of: pFN21AE1990.seq Check: 3332 from: 1 to: 6406
pFN21AE1990 6406 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3036 3749 1937
Size: 1099 713 4594
Fragments arranged by size:
4594 1099 713
Acc65I G'GTAC_C
Cuts at: 3727 3727
Size: 6406
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2279 2468 2663 4469 5853 6121 1632
Size: 647 189 195 1806 1384 268 1917
Fragments arranged by size:
1917 1806 1384 647 268 195 189
AfeI AGC'GCT
Cuts at: 2309 2309
Size: 6406
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 2463 5305 6282 1203
Size: 1260 2842 977 1327
Fragments arranged by size:
2842 1327 1260 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AlwNI CAG_nnn'CTG
Cuts at: 2395 3280 3322 4149 5721 2395
Size: 885 42 827 1572 3080
Fragments arranged by size:
3080 1572 885 827 42
ApaI G_GGCC'C
Cuts at: 2094 2200 3547 3621 2094
Size: 106 1347 74 4879
Fragments arranged by size:
4879 1347 106 74
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 3593 3715 3941 4093 6202 1493
Size: 2100 122 226 152 2109 1697
Fragments arranged by size:
2109 2100 1697 226 152 122
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2648 3731 1870
Size: 73 705 1083 4545
Fragments arranged by size:
4545 1083 705 73
BamHI G'GATC_C
Cuts at: 3674 3736 4103 3674
Size: 62 367 5977
Fragments arranged by size:
5977 367 62
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3460 3727 5182 618
Size: 325 217 627 1673 267 1455 1842
Fragments arranged by size:
1842 1673 1455 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 2094 2200 2402 2454 2792 3239 3305 3547
Size: 703 106 202 52 338 447 66 242
Cuts at: 3547 3621 3725 1391
Size: 74 104 4072
Fragments arranged by size:
4072 703 447 338 242 202 106 104
74 66 52
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5435
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2498 2532 4597 4631 1903
Size: 34 50 34 477 34 2065 34 3678
Fragments arranged by size:
3678 2065 477 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2195 2948 5017
Size: 107 122 71 631 605 523 753 2069
Cuts at: 5017 136
Size: 1525
Fragments arranged by size:
2069 1525 753 631 605 523 122 107
71
BglII A'GATC_T
Cuts at: 2487 3351 6401 2487
Size: 864 3050 2492
Fragments arranged by size:
3050 2492 864
BlpI GC'TnA_GC
Cuts at: 2075 2396 3823 2075
Size: 321 1427 4658
Fragments arranged by size:
4658 1427 321
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2200 2476 3465 3547 3621 4467 5623
Size: 304 106 276 989 82 74 846 1156
Cuts at: 5623 1790
Size: 2573
Fragments arranged by size:
2573 1156 989 846 304 276 106 82
74
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2139 2396 3438
Size: 416 53 83 399 24 722 257 1042
Cuts at: 3438 3459 4137 5095 442
Size: 21 678 958 1753
Fragments arranged by size:
1753 1042 958 722 678 416 399 257
83 53 24 21
BmtI G_CTAG'C
Cuts at: 1056 2348 2561 1056
Size: 1292 213 4901
Fragments arranged by size:
4901 1292 213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3158 3173 3239 3417 5066 1239
Size: 1919 15 66 178 1649 2579
Fragments arranged by size:
2579 1919 1649 178 66 15
Bpu10I CC'TnA_GC
Cuts at: 2349 2349
Size: 6406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3253 4531 5396 5694 3253
Size: 1278 865 298 3965
Fragments arranged by size:
3965 1278 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3626 5069 882
Size: 617 2127 1443 2219
Fragments arranged by size:
2219 2127 1443 617
BsaAI yAC'GTr
Cuts at: 493 1204 4197 493
Size: 711 2993 2702
Fragments arranged by size:
2993 2702 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2575 2619 4213
Size: 53 83 186 936 498 544 44 1594
Cuts at: 4213 4595 5230 275
Size: 382 635 1451
Fragments arranged by size:
1594 1451 936 635 544 498 382 186
83 53 44
BsaWI w'CCGG_w
Cuts at: 2859 4839 5237 5511 5658 6069 2859
Size: 1980 398 274 147 411 3196
Fragments arranged by size:
3196 1980 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2366 2396 1178
Size: 30 165 30 963 30 5188
Fragments arranged by size:
5188 963 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2189 2450 2587 2690 2791 3644 1186
Size: 1003 261 137 103 101 853 3948
Fragments arranged by size:
3948 1003 853 261 137 103 101
BseYI C'CCAG_C
Cuts at: 2153 2193 2230 2337 2665 2746 3620 3660 5609
Size: 40 37 107 328 81 874 40 1949
Cuts at: 5609 6105 2153
Size: 496 2454
Fragments arranged by size:
2454 1949 874 496 328 107 81 40
40 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3067 3239 3551 1163
Size: 573 1331 172 312 4018
Fragments arranged by size:
4018 1331 573 312 172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2416 2459 3856 4617 5645 1540
Size: 275 186 415 43 1397 761 1028 2301
Fragments arranged by size:
2301 1397 1028 761 415 275 186 43
BsiHKAI G_wGCw'C
Cuts at: 2879 3305 4467 4552 5623 2879
Size: 426 1162 85 1071 3662
Fragments arranged by size:
3662 1162 1071 426 85
BsmI GAATG_Cn'
Cuts at: 3917 4010 3917
Size: 93 6313
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2200 2402 2454 2476 2792 2879
Size: 399 304 106 202 52 22 316 87
Cuts at: 2879 3239 3305 3465 3547 3621 3725 4467 4552
Size: 360 66 160 82 74 104 742 85
Cuts at: 4552 5623 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 316 304 202
160 106 104 87 85 82 74 66
52 22
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2934 3761 844
Size: 2090 827 3489
Fragments arranged by size:
3489 2090 827
BsrBI CCG'CTC
Cuts at: 1394 1823 2212 4293 1394
Size: 429 389 2081 3507
Fragments arranged by size:
3507 2081 429 389
BsrDI GCAATG_nn'
Cuts at: 65 1248 3055 4901 5075 65
Size: 1183 1807 1846 174 1396
Fragments arranged by size:
1846 1807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2898 3258 5050 5237 1700
Size: 110 1088 360 1792 187 2869
Fragments arranged by size:
2869 1792 1088 360 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 2519 1925
Size: 594 5812
BssSI C'ACGA_G
Cuts at: 2623 4466 5478 2623
Size: 1843 1012 3551
Fragments arranged by size:
3551 1843 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6406
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6406
BstXI CCAn_nnnn'nTGG
Cuts at: 2026 3451 2026
Size: 1425 4981
BstYI r'GATC_y
Cuts at: 1972 2319 2487 2994 3283 3351 3674 3736 4103
Size: 347 168 507 289 68 323 62 367
Cuts at: 4103 4488 4505 5957 6043 6055 6401 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 507 385 367 347 346 323
289 168 86 68 62 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2113 513
Size: 552 1048 4806
Fragments arranged by size:
4806 1048 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 3922 4716 4736 3922
Size: 794 20 5592
Fragments arranged by size:
5592 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3032 5413 809
Size: 507 1716 2381 1802
Fragments arranged by size:
2381 1802 1716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2325 2445
Size: 54 1425 50 275 18 357 138 120
Cuts at: 2445 2456 2900 2955 3260 3853 4742 6371 8
Size: 11 444 55 305 593 889 1629 43
Fragments arranged by size:
1629 1425 889 593 444 357 305 275
138 120 55 54 50 43 18 11
EagI C'GGCC_G
Cuts at: 1537 1812 2456 3853 1537
Size: 275 644 1397 4090
Fragments arranged by size:
4090 1397 644 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2858 3342 4336 1353
Size: 1505 484 994 3423
Fragments arranged by size:
3423 1505 994 484
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2726 2794 3110 4993 5367 5513
Size: 1064 138 1137 68 316 1883 374 146
Cuts at: 5513 387
Size: 1280
Fragments arranged by size:
1883 1280 1137 1064 374 316 146 138
68
EcoICRI GAG'CTC
Cuts at: 3303 3303
Size: 6406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2468 2663 3158 3173 3239 3417
Size: 393 647 189 195 495 15 66 178
Cuts at: 3417 4469 5066 5853 6121 1239
Size: 1052 597 787 268 1524
Fragments arranged by size:
1524 1052 787 647 597 495 393 268
195 189 178 66 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 3074 2261
Size: 813 5593
EcoO109I rG'GnC_Cy
Cuts at: 2090 2091 2197 2224 2705 3061 3543 3559 3577
Size: 1 106 27 481 356 482 16 18
Cuts at: 3577 3617 2090
Size: 40 4879
Fragments arranged by size:
4879 482 481 356 106 40 27 18
16 1
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3247 3247
Size: 6406
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 2311 2396 3152 3206 5553 1678
Size: 633 85 756 54 2347 2531
Fragments arranged by size:
2531 2347 756 633 85 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 2952 2984 5047
Size: 32 135 32 794 32 554 32 2063
Cuts at: 5047 5079 5121 5153 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2063 794 554 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 3996 669
Size: 988 281 1812 246 3079
Fragments arranged by size:
3079 1812 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 3731 3731
Size: 6406
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5520 5704 1305
Size: 4215 184 2007
Fragments arranged by size:
4215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2189 2957 10
Size: 54 1425 343 357 768 3459
Fragments arranged by size:
3459 1425 768 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4364 4723 4882 518
Size: 648 3198 359 159 2042
Fragments arranged by size:
3198 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2115 2193 2230 2539 2665 2689 3540 3604
Size: 509 78 37 309 126 24 851 64
Cuts at: 3604 3822 4499 5647 5892 1606
Size: 218 677 1148 245 2120
Fragments arranged by size:
2120 1148 851 677 509 309 245 218
126 78 64 37 24
NaeI GCC'GGC
Cuts at: 2900 2900
Size: 6406
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5854
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NgoMIV G'CCGG_C
Cuts at: 2898 2898
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 2344 2557 1052
Size: 1292 213 4901
Fragments arranged by size:
4901 1292 213
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 2467 2986 3764 5309 1132
Size: 97 1238 519 778 1545 2229
Fragments arranged by size:
2229 1545 1238 778 519 97
PasI CC'CwG_GG
Cuts at: 1348 2095 1348
Size: 747 5659
PciI A'CATG_T
Cuts at: 2463 5305 2463
Size: 2842 3564
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3322 1108
Size: 2214 4192
PfoI T'CCnGG_A
Cuts at: 3069 3069
Size: 6406
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2224 2705 3577 2224
Size: 481 872 5053
Fragments arranged by size:
5053 872 481
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PspOMI G'GGCC_C
Cuts at: 2090 2196 3543 3617 2090
Size: 106 1347 74 4879
Fragments arranged by size:
4879 1347 106 74
PstI C_TGCA'G
Cuts at: 830 3198 3210 3603 3758 830
Size: 2368 12 393 155 3478
Fragments arranged by size:
3478 2368 393 155 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2193 2689 3604 2193
Size: 496 915 4995
Fragments arranged by size:
4995 915 496
SacI G_AGCT'C
Cuts at: 3305 3305
Size: 6406
SacII CC_GC'GG
Cuts at: 2116 2116
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SapI GCTCTTCn'nnn_
Cuts at: 2858 2858
Size: 6406
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 2912 4654 6366 2912
Size: 1742 1712 2952
Fragments arranged by size:
2952 1742 1712
SexAI A'CCwGG_T
Cuts at: 3039 3579 3039
Size: 540 5866
SfcI C'TryA_G
Cuts at: 826 1045 3194 3206 3599 3754 4889 5570 5761
Size: 219 2149 12 393 155 1135 681 191
Cuts at: 5761 826
Size: 1471
Fragments arranged by size:
2149 1471 1135 681 393 219 191 155
12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2195 2195
Size: 6406
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 2650 3733 1872
Size: 778 1083 4545
Fragments arranged by size:
4545 1083 778
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3232 4510 5411 5673 820
Size: 197 926 1289 1278 901 262 1553
Fragments arranged by size:
1553 1289 1278 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 2300 2423 3012 3507 1517
Size: 783 123 589 495 4416
Fragments arranged by size:
4416 783 589 495 123
StyI C'CwwG_G
Cuts at: 513 1065 2818 2869 2946 3613 3845 513
Size: 552 1753 51 77 667 232 3074
Fragments arranged by size:
3074 1753 667 552 232 77 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2657 3437 4433 4450 4603 4788 5233
Size: 1255 1077 780 996 17 153 185 445
Cuts at: 5233 325
Size: 1498
Fragments arranged by size:
1498 1255 1077 996 780 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2910 4652 6364
Size: 274 80 33 51 1443 933 1742 1712
Cuts at: 6364 96
Size: 138
Fragments arranged by size:
1742 1712 1443 933 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6366 6398 6366
Size: 32 6374
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2044 2044
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 2648 3731 1870
Size: 778 1083 4545
Fragments arranged by size:
4545 1083 778
XmnI GAAnn'nnTTC
Cuts at: 2267 4535 2267
Size: 2268 4138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI RsrII SanDI SfoI SrfI SwaI