Restriction map for
pFN21AE1988
(Circular) (Six-base) MAPSORT of: pFN21AE1988.seq Check: 5216 from: 1 to: 6397
pFN21AE1988 6397 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4207 278
Size: 53 83 186 936 2671 2468
Fragments arranged by size:
2671 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3138 3740 1937
Size: 1201 602 4594
Fragments arranged by size:
4594 1201 602
Acc65I G'GTAC_C
Cuts at: 3718 3718
Size: 6397
AclI AA'CG_TT
Cuts at: 1563 4524 4897 1563
Size: 2961 373 3063
Fragments arranged by size:
3063 2961 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4460 5844 6112 1632
Size: 2828 1384 268 1917
Fragments arranged by size:
2828 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6200
AflIII A'CryG_T
Cuts at: 1203 5296 6273 1203
Size: 4093 977 1327
Fragments arranged by size:
4093 1327 977
AgeI A'CCGG_T
Cuts at: 5228 5228
Size: 6397
AhdI GACnn_n'nnGTC
Cuts at: 5126 5126
Size: 6397
AleI CACnn'nnGTG
Cuts at: 1166 2066 1166
Size: 900 5497
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6397
AlwNI CAG_nnn'CTG
Cuts at: 2626 4140 5712 2626
Size: 1514 1572 3311
Fragments arranged by size:
3311 1572 1514
ApaLI G'TGCA_C
Cuts at: 4454 5610 4454
Size: 1156 5241
ApoI r'AATT_y
Cuts at: 1493 2219 2670 3281 3706 3932 4084 6193 1493
Size: 726 451 611 425 226 152 2109 1697
Fragments arranged by size:
2109 1697 726 611 451 425 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4951 160
Size: 872 3919 1606
Fragments arranged by size:
3919 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6397
AvaI C'yCGr_G
Cuts at: 1870 1943 2265 2508 3722 1870
Size: 73 322 243 1214 4545
Fragments arranged by size:
4545 1214 322 243 73
BamHI G'GATC_C
Cuts at: 3727 4094 3727
Size: 367 6030
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3658 3718 5173 618
Size: 325 217 627 1871 60 1455 1842
Fragments arranged by size:
1871 1842 1455 627 325 217 60
BanII G_rGCy'C
Cuts at: 1391 2029 3212 3716 1391
Size: 638 1183 504 4072
Fragments arranged by size:
4072 1183 638 504
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 928
Size: 971 402 5024
Fragments arranged by size:
5024 971 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4588 4622 1903
Size: 34 2651 34 3678
Fragments arranged by size:
3678 2651 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4289 5499 4289
Size: 1210 5187
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6397
BfrBI ATG'CAT
Cuts at: 2365 2365
Size: 6397
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5008 136
Size: 107 122 71 631 605 3336 1525
Fragments arranged by size:
3336 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2470 2512 6392 2470
Size: 42 3880 2475
Fragments arranged by size:
3880 2475 42
BlpI GC'TnA_GC
Cuts at: 3814 3814
Size: 6397
Bme1580I G_kGCm'C
Cuts at: 1790 3661 4458 5614 1790
Size: 1871 797 1156 2573
Fragments arranged by size:
2573 1871 1156 797
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6397
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4128 5086 442
Size: 416 53 83 399 24 2711 958 1753
Fragments arranged by size:
2711 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6397
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2873 2908 5057 1239
Size: 1076 558 35 2149 2579
Fragments arranged by size:
2579 2149 1076 558 35
Bpu10I CC'TnA_GC
Cuts at: 3288 3288
Size: 6397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4522 5387 5685 4522
Size: 865 298 5234
Fragments arranged by size:
5234 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2511 5060 882
Size: 617 1012 2549 2219
Fragments arranged by size:
2549 2219 1012 617
BsaAI yAC'GTr
Cuts at: 493 1204 4188 493
Size: 711 2984 2702
Fragments arranged by size:
2984 2702 711
BsaBI GATnn'nnATC
Cuts at: 6391 6391
Size: 6397
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3141 4204 4586 5221
Size: 53 83 186 936 1608 1063 382 635
Cuts at: 5221 275
Size: 1451
Fragments arranged by size:
1608 1451 1063 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4830 5228 5502 5649 6060 4830
Size: 398 274 147 411 5167
Fragments arranged by size:
5167 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3497 3527 1178
Size: 30 165 30 2094 30 4048
Fragments arranged by size:
4048 2094 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2364 3008 3098 3505 3621 3654 3669 1186
Size: 1178 644 90 407 116 33 15 3914
Fragments arranged by size:
3914 1178 644 407 116 90 33 15
BseYI C'CCAG_C
Cuts at: 3581 5600 6096 3581
Size: 2019 496 3882
Fragments arranged by size:
3882 2019 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2156 2721 2868 3028 1163
Size: 573 420 565 147 160 4532
Fragments arranged by size:
4532 573 565 420 160 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3847 4608 5636 1540
Size: 275 186 1846 761 1028 2301
Fragments arranged by size:
2301 1846 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 4458 4543 5614 2073
Size: 2385 85 1071 2856
Fragments arranged by size:
2856 2385 1071 85
BsmI GAATG_Cn'
Cuts at: 3253 3908 4001 3253
Size: 655 93 5649
Fragments arranged by size:
5649 655 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2073 3212 3661 3716 4458 4543
Size: 399 239 44 1139 449 55 742 85
Cuts at: 4543 5614 1391
Size: 1071 2174
Fragments arranged by size:
2174 1139 1071 742 449 399 239 85
55 44
BspEI T'CCGG_A
Cuts at: 6060 6060
Size: 6397
BspHI T'CATG_A
Cuts at: 4286 6016 4286
Size: 1730 4667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3752 844
Size: 2908 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2020 4284 1394
Size: 429 197 2264 3507
Fragments arranged by size:
3507 2264 429 197
BsrDI GCAATG_nn'
Cuts at: 65 1248 2439 4892 5066 65
Size: 1183 1191 2453 174 1396
Fragments arranged by size:
2453 1396 1191 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5041 5228 1700
Size: 110 3231 187 2869
Fragments arranged by size:
3231 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6397
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6397
BssSI C'ACGA_G
Cuts at: 4457 5469 4457
Size: 1012 5385
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3248 794
Size: 2454 3943
BstBI TT'CG_AA
Cuts at: 5201 5201
Size: 6397
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6397
BstYI r'GATC_y
Cuts at: 1972 2470 2512 3727 4094 4479 4496 5948 6034
Size: 498 42 1215 367 385 17 1452 86
Cuts at: 6034 6046 6392 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1215 498 385 367 346 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 513
Size: 552 939 6 4900
Fragments arranged by size:
4900 939 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2962 487
Size: 683 56 1736 3922
Fragments arranged by size:
3922 1736 683 56
BtsI GCAGTG_nn'
Cuts at: 3562 3913 4707 4727 3562
Size: 351 794 20 5232
Fragments arranged by size:
5232 794 351 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6397
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6362
DraI TTT'AAA
Cuts at: 3701 3701
Size: 6397
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5404 809
Size: 507 4088 1802
Fragments arranged by size:
4088 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2058 2973
Size: 54 1425 50 275 18 177 51 915
Cuts at: 2973 3128 3844 4733 6362 8
Size: 155 716 889 1629 43
Fragments arranged by size:
1629 1425 915 889 716 275 177 155
54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3844 1537
Size: 275 2032 4090
Fragments arranged by size:
4090 2032 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4327 1353
Size: 2974 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 2142 4984 5358 5504 387
Size: 1064 138 441 112 2842 374 146 1280
Fragments arranged by size:
2842 1280 1064 441 374 146 138 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2873 2908 4460 5057 5844 6112
Size: 393 683 558 35 1552 597 787 268
Cuts at: 6112 1239
Size: 1524
Fragments arranged by size:
1552 1524 787 683 597 558 393 268
35
EcoO109I rG'GnC_Cy
Cuts at: 2767 3064 3379 2767
Size: 297 315 5785
Fragments arranged by size:
5785 315 297
EcoRI G'AATT_C
Cuts at: 3281 3706 4084 3281
Size: 425 378 5594
Fragments arranged by size:
5594 425 378
FspI TGC'GCA
Cuts at: 4903 4903
Size: 6397
HaeII r_GCGC'y
Cuts at: 1678 2855 3445 3588 5544 1678
Size: 1177 590 143 1956 2531
Fragments arranged by size:
2531 1956 1177 590 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2292 2324 2897 2929 3392
Size: 32 135 32 720 32 573 32 463
Cuts at: 3392 3424 5038 5070 5112 5144 1373
Size: 32 1614 32 42 32 2626
Fragments arranged by size:
2626 1614 720 573 463 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3741 3987 669
Size: 988 281 1803 246 3079
Fragments arranged by size:
3079 1803 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6397
HpaI GTT'AAC
Cuts at: 3987 3987
Size: 6397
KpnI G_GTAC'C
Cuts at: 3722 3722
Size: 6397
MfeI C'AATT_G
Cuts at: 2523 3996 2523
Size: 1473 4924
MluI A'CGCG_T
Cuts at: 6273 6273
Size: 6397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2767 3148 3213 5511 5695 1305
Size: 1462 381 65 2298 184 2007
Fragments arranged by size:
2298 2007 1462 381 184 65
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2060 2975 3130 10
Size: 54 1425 343 177 51 915 155 3277
Fragments arranged by size:
3277 1425 915 343 177 155 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2181 2360 4355 4714 4873 518
Size: 648 900 115 179 1995 359 159 2042
Fragments arranged by size:
2042 1995 900 648 359 179 159 115
MspA1I CmG'CkG
Cuts at: 1606 2566 2681 3813 4490 5638 5883 1606
Size: 960 115 1132 677 1148 245 2120
Fragments arranged by size:
2120 1148 1132 960 677 245 115
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4906
Fragments arranged by size:
4906 939 552
NdeI CA'TA_TG
Cuts at: 387 2696 387
Size: 2309 4088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6397
NotI GC'GGCC_GC
Cuts at: 3844 3844
Size: 6397
NsiI A_TGCA'T
Cuts at: 2367 2367
Size: 6397
NspI r_CATG'y
Cuts at: 1132 1229 2365 2750 3755 5300 1132
Size: 97 1136 385 1005 1545 2229
Fragments arranged by size:
2229 1545 1136 1005 385 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6397
PciI A'CATG_T
Cuts at: 5296 5296
Size: 6397
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6397
PmeI GTTT'AAAC
Cuts at: 3701 3701
Size: 6397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4466 4498 5979 6011 4466
Size: 32 1481 32 4852
Fragments arranged by size:
4852 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2767 3064 3379 2767
Size: 297 315 5785
Fragments arranged by size:
5785 315 297
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6397
PsiI TTA'TAA
Cuts at: 3967 3967
Size: 6397
PstI C_TGCA'G
Cuts at: 830 2777 3749 830
Size: 1947 972 3478
Fragments arranged by size:
3478 1947 972
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6397
PvuII CAG'CTG
Cuts at: 2566 2681 2566
Size: 115 6282
SalI G'TCGA_C
Cuts at: 1936 3739 1936
Size: 1803 4594
SbfI CC_TGCA'GG
Cuts at: 3749 3749
Size: 6397
ScaI AGT'ACT
Cuts at: 4645 6357 4645
Size: 1712 4685
SfcI C'TryA_G
Cuts at: 826 1045 2773 3745 4880 5561 5752 826
Size: 219 1728 972 1135 681 191 1471
Fragments arranged by size:
1728 1471 1135 972 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6397
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6397
SmaI CCC'GGG
Cuts at: 1872 3724 1872
Size: 1852 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2508 4501 5402 5664 820
Size: 197 926 565 1993 901 262 1553
Fragments arranged by size:
1993 1553 926 901 565 262 197
SnaBI TAC'GTA
Cuts at: 493 4188 493
Size: 3695 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6397
SphI G_CATG'C
Cuts at: 1132 3755 1132
Size: 2623 3774
SspI AAT'ATT
Cuts at: 5 52 4321 5
Size: 47 4269 2081
Fragments arranged by size:
4269 2081 47
StuI AGG'CCT
Cuts at: 1517 2319 3688 1517
Size: 802 1369 4226
Fragments arranged by size:
4226 1369 802
StyI C'CwwG_G
Cuts at: 513 1065 2004 3683 3836 513
Size: 552 939 1679 153 3074
Fragments arranged by size:
3074 1679 939 552 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2785 4424 4441 4594 4779 5224 325
Size: 1255 1205 1639 17 153 185 445 1498
Fragments arranged by size:
1639 1498 1255 1205 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 4643 6355
Size: 274 80 33 51 1443 162 2504 1712
Cuts at: 6355 96
Size: 138
Fragments arranged by size:
2504 1712 1443 274 162 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6357 6389 6357
Size: 32 6365
Tth111I GACn'n_nGTC
Cuts at: 1316 2765 2795 1316
Size: 1449 30 4918
Fragments arranged by size:
4918 1449 30
XbaI T'CTAG_A
Cuts at: 3733 3733
Size: 6397
XhoI C'TCGA_G
Cuts at: 1943 2508 1943
Size: 565 5832
XmaI C'CCGG_G
Cuts at: 1870 3722 1870
Size: 1852 4545
XmnI GAAnn'nnTTC
Cuts at: 2549 4526 2549
Size: 1977 4420
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4205 276
Size: 53 83 186 936 2671 2468
Fragments arranged by size:
2671 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI