Restriction map for
pFN21AE1983
(Circular) (Six-base) MAPSORT of: pFN21AE1983.seq Check: 8272 from: 1 to: 6382
pFN21AE1983 6382 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4192 278
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3725 1937
Size: 1788 4594
Acc65I G'GTAC_C
Cuts at: 3703 3703
Size: 6382
AclI AA'CG_TT
Cuts at: 1563 4509 4882 1563
Size: 2946 373 3063
Fragments arranged by size:
3063 2946 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2288 2433 4445 5829 6097 1632
Size: 656 145 2012 1384 268 1917
Fragments arranged by size:
2012 1917 1384 656 268 145
AfeI AGC'GCT
Cuts at: 2286 2286
Size: 6382
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6185
AflIII A'CryG_T
Cuts at: 1203 5281 6258 1203
Size: 4078 977 1327
Fragments arranged by size:
4078 1327 977
AgeI A'CCGG_T
Cuts at: 5213 5213
Size: 6382
AhdI GACnn_n'nnGTC
Cuts at: 5111 5111
Size: 6382
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2612 1184
Size: 1428 4954
AlwNI CAG_nnn'CTG
Cuts at: 2585 4125 5697 2585
Size: 1540 1572 3270
Fragments arranged by size:
3270 1572 1540
ApaLI G'TGCA_C
Cuts at: 4439 5595 4439
Size: 1156 5226
ApoI r'AATT_y
Cuts at: 1493 3109 3492 3691 3917 4069 6178 1493
Size: 1616 383 199 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 383 226 199 152
AseI AT'TA_AT
Cuts at: 160 1032 4936 160
Size: 872 3904 1606
Fragments arranged by size:
3904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
AvaI C'yCGr_G
Cuts at: 1870 1943 3707 1870
Size: 73 1764 4545
Fragments arranged by size:
4545 1764 73
BamHI G'GATC_C
Cuts at: 3712 4079 3712
Size: 367 6015
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3703 5158 618
Size: 325 217 627 1916 1455 1842
Fragments arranged by size:
1916 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2328 2616 3210 3701 1391
Size: 937 288 594 491 4072
Fragments arranged by size:
4072 937 594 491 288
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3261 928
Size: 971 1362 4049
Fragments arranged by size:
4049 1362 971
BbvCI CC'TCA_GC
Cuts at: 2521 2521
Size: 6382
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4573 4607 1903
Size: 34 2636 34 3678
Fragments arranged by size:
3678 2636 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4274 5484 4274
Size: 1210 5172
BclI T'GATC_A
Cuts at: 1548 3102 3397 1548
Size: 1554 295 4533
Fragments arranged by size:
4533 1554 295
BfrBI ATG'CAT
Cuts at: 3647 3647
Size: 6382
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4993 136
Size: 107 122 71 631 605 3321 1525
Fragments arranged by size:
3321 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6377 6377
Size: 6382
BlpI GC'TnA_GC
Cuts at: 3338 3799 3338
Size: 461 5921
Bme1580I G_kGCm'C
Cuts at: 1790 4443 5599 1790
Size: 2653 1156 2573
Fragments arranged by size:
2653 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6382
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2493 4113 5071
Size: 416 53 83 399 24 1076 1620 958
Cuts at: 5071 442
Size: 1753
Fragments arranged by size:
1753 1620 1076 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2342 2342
Size: 6382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 2366 2687 2764 3386 5042 1239
Size: 1070 57 321 77 622 1656 2579
Fragments arranged by size:
2579 1656 1070 622 321 77 57
Bpu10I CC'TnA_GC
Cuts at: 2521 2626 3072 3224 2521
Size: 105 446 152 5679
Fragments arranged by size:
5679 446 152 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4507 5372 5670 4507
Size: 865 298 5219
Fragments arranged by size:
5219 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2170 2503 2841 2961 3454 5045 882
Size: 617 671 333 338 120 493 1591 2219
Fragments arranged by size:
2219 1591 671 617 493 338 333 120
BsaAI yAC'GTr
Cuts at: 493 1204 3517 4173 493
Size: 711 2313 656 2702
Fragments arranged by size:
2702 2313 711 656
BsaBI GATnn'nnATC
Cuts at: 6376 6376
Size: 6382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 4189 4571 5206
Size: 53 83 186 936 573 2083 382 635
Cuts at: 5206 275
Size: 1451
Fragments arranged by size:
2083 1451 936 635 573 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3258 4815 5213 5487 5634 6045 3258
Size: 1557 398 274 147 411 3595
Fragments arranged by size:
3595 1557 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2483 2513 2821 2851 1178
Size: 30 165 30 1080 30 308 30 4709
Fragments arranged by size:
4709 1080 308 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 2086 2206 2336 2903 3038 3098 3164
Size: 888 12 120 130 567 135 60 66
Cuts at: 3164 1186
Size: 4404
Fragments arranged by size:
4404 888 567 135 130 120 66 60
12
BseYI C'CCAG_C
Cuts at: 2066 2460 2630 2690 5585 6081 2066
Size: 394 170 60 2895 496 2367
Fragments arranged by size:
2895 2367 496 394 170 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2879 1163
Size: 573 1143 4666
Fragments arranged by size:
4666 1143 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3832 4593 5621 1540
Size: 275 186 1831 761 1028 2301
Fragments arranged by size:
2301 1831 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2328 2616 3210 4443 4528 5599 2328
Size: 288 594 1233 85 1071 3111
Fragments arranged by size:
3111 1233 1071 594 288 85
BsmI GAATG_Cn'
Cuts at: 3893 3986 3893
Size: 93 6289
BsmBI CGTCTCn'nnnn_
Cuts at: 2432 2432
Size: 6382
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2328 2616 3210 3701 4443 4528 5599
Size: 399 538 288 594 491 742 85 1071
Cuts at: 5599 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 594 538 491 399 288
85
BspEI T'CCGG_A
Cuts at: 6045 6045
Size: 6382
BspHI T'CATG_A
Cuts at: 4271 6001 4271
Size: 1730 4652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3358 3737 844
Size: 2514 379 3489
Fragments arranged by size:
3489 2514 379
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 2052 3007 4269 1394
Size: 429 205 24 955 1262 3507
Fragments arranged by size:
3507 1262 955 429 205 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 4877 5051 65
Size: 1183 3629 174 1396
Fragments arranged by size:
3629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2303 2859 3231 5026 5213 1700
Size: 110 493 556 372 1795 187 2869
Fragments arranged by size:
2869 1795 556 493 372 187 110
BsrGI T'GTAC_A
Cuts at: 96 2407 96
Size: 2311 4071
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6382
BssSI C'ACGA_G
Cuts at: 2036 4442 5454 2036
Size: 2406 1012 2964
Fragments arranged by size:
2964 2406 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2379 794
Size: 1585 4797
BstBI TT'CG_AA
Cuts at: 2176 5186 2176
Size: 3010 3372
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6382
BstXI CCAn_nnnn'nTGG
Cuts at: 2665 2665
Size: 6382
BstYI r'GATC_y
Cuts at: 1972 2223 2672 3300 3712 4079 4464 4481 5933
Size: 251 449 628 412 367 385 17 1452
Cuts at: 5933 6019 6031 6377 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 628 449 412 385 367 346
251 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2100 2676 2829 2850 3188 3354
Size: 552 939 96 576 153 21 338 166
Cuts at: 3354 513
Size: 3541
Fragments arranged by size:
3541 939 576 552 338 166 153 96
21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5643
Fragments arranged by size:
5643 683 56
BtsI GCAGTG_nn'
Cuts at: 2699 3182 3898 4692 4712 2699
Size: 483 716 794 20 4369
Fragments arranged by size:
4369 794 716 483 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6347
DraI TTT'AAA
Cuts at: 3686 3686
Size: 6382
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2069 3574 5389 809
Size: 507 753 1505 1815 1802
Fragments arranged by size:
1815 1802 1505 753 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2088 2208
Size: 54 1425 50 275 18 177 81 120
Cuts at: 2208 2301 2478 2907 3213 3233 3829 4718 6347
Size: 93 177 429 306 20 596 889 1629
Cuts at: 6347 8
Size: 43
Fragments arranged by size:
1629 1425 889 596 429 306 275 177
177 120 93 81 54 50 43 20
18
EagI C'GGCC_G
Cuts at: 1537 1812 3829 1537
Size: 275 2017 4090
Fragments arranged by size:
4090 2017 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2774 2783 2871 2935 3354 3415 4312 1353
Size: 1421 9 88 64 419 61 897 3423
Fragments arranged by size:
3423 1421 897 419 88 64 61 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2703 4969 5343 5489 387
Size: 1064 138 1114 2266 374 146 1280
Fragments arranged by size:
2266 1280 1114 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2326 2614 3208 2326
Size: 288 594 5500
Fragments arranged by size:
5500 594 288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2288 2309 2366 2433 2687 2764 3386
Size: 393 656 21 57 67 254 77 622
Cuts at: 3386 4445 5042 5829 6097 1239
Size: 1059 597 787 268 1524
Fragments arranged by size:
1524 1059 787 656 622 597 393 268
254 77 67 57 21
EcoO109I rG'GnC_Cy
Cuts at: 2396 2396
Size: 6382
EcoRI G'AATT_C
Cuts at: 3691 4069 3691
Size: 378 6004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2394 2394
Size: 6382
FspI TGC'GCA
Cuts at: 4888 4888
Size: 6382
HaeII r_GCGC'y
Cuts at: 1678 2288 2906 3158 5529 1678
Size: 610 618 252 2371 2531
Fragments arranged by size:
2531 2371 618 610 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 2483 2515 3285
Size: 32 135 32 592 32 287 32 770
Cuts at: 3285 3317 5023 5055 5097 5129 1373
Size: 32 1706 32 42 32 2626
Fragments arranged by size:
2626 1706 770 592 287 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3726 3972 669
Size: 988 281 1788 246 3079
Fragments arranged by size:
3079 1788 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3323 3449 748
Size: 2575 126 3681
Fragments arranged by size:
3681 2575 126
HpaI GTT'AAC
Cuts at: 3972 3972
Size: 6382
KpnI G_GTAC'C
Cuts at: 3707 3707
Size: 6382
MfeI C'AATT_G
Cuts at: 3981 3981
Size: 6382
MluI A'CGCG_T
Cuts at: 6258 6258
Size: 6382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1995 2415 5496 5680 1305
Size: 690 420 3081 184 2007
Fragments arranged by size:
3081 2007 690 420 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 2480 2909 10
Size: 54 1425 343 378 270 429 3483
Fragments arranged by size:
3483 1425 429 378 343 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3193 4340 4699 4858 518
Size: 648 2027 1147 359 159 2042
Fragments arranged by size:
2042 2027 1147 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2102 2525 3798 4475 5623 5868 1606
Size: 496 423 1273 677 1148 245 2120
Fragments arranged by size:
2120 1273 1148 677 496 423 245
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 6382
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4891
Fragments arranged by size:
4891 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6382
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 6382
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6382
NotI GC'GGCC_GC
Cuts at: 3829 3829
Size: 6382
NsiI A_TGCA'T
Cuts at: 3649 3649
Size: 6382
NspI r_CATG'y
Cuts at: 1132 1229 3647 3740 5285 1132
Size: 97 2418 93 1545 2229
Fragments arranged by size:
2418 2229 1545 97 93
PasI CC'CwG_GG
Cuts at: 1348 2215 1348
Size: 867 5515
PciI A'CATG_T
Cuts at: 5281 5281
Size: 6382
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6382
PmeI GTTT'AAAC
Cuts at: 3686 3686
Size: 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4451 4483 5964 5996 4451
Size: 32 1481 32 4837
Fragments arranged by size:
4837 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2396 2396
Size: 6382
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6382
PsiI TTA'TAA
Cuts at: 3952 3952
Size: 6382
PstI C_TGCA'G
Cuts at: 830 3734 830
Size: 2904 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6382
PvuII CAG'CTG
Cuts at: 2525 2525
Size: 6382
SacI G_AGCT'C
Cuts at: 2328 2616 3210 2328
Size: 288 594 5500
Fragments arranged by size:
5500 594 288
SacII CC_GC'GG
Cuts at: 2103 2103
Size: 6382
SalI G'TCGA_C
Cuts at: 1936 3724 1936
Size: 1788 4594
SapI GCTCTTCn'nnn_
Cuts at: 3354 3354
Size: 6382
SbfI CC_TGCA'GG
Cuts at: 3734 3734
Size: 6382
ScaI AGT'ACT
Cuts at: 4630 6342 4630
Size: 1712 4670
SexAI A'CCwGG_T
Cuts at: 2493 2493
Size: 6382
SfcI C'TryA_G
Cuts at: 826 1045 3730 4865 5546 5737 826
Size: 219 2685 1135 681 191 1471
Fragments arranged by size:
2685 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6382
SmaI CCC'GGG
Cuts at: 1872 3709 1872
Size: 1837 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4486 5387 5649 820
Size: 197 926 2543 901 262 1553
Fragments arranged by size:
2543 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4173 493
Size: 3680 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6382
SphI G_CATG'C
Cuts at: 1132 3740 1132
Size: 2608 3774
SspI AAT'ATT
Cuts at: 5 52 4306 5
Size: 47 4254 2081
Fragments arranged by size:
4254 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6382
StyI C'CwwG_G
Cuts at: 513 1065 2004 2475 2721 3821 513
Size: 552 939 471 246 1100 3074
Fragments arranged by size:
3074 1100 939 552 471 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2437 2734 2842 4409 4426 4579 4764
Size: 1255 857 297 108 1567 17 153 185
Cuts at: 4764 5209 325
Size: 445 1498
Fragments arranged by size:
1567 1498 1255 857 445 297 185 153
108 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2407 3618 4628
Size: 274 80 33 51 1443 430 1211 1010
Cuts at: 4628 6340 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1211 1010 430 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2821 2853 6342 6374 2821
Size: 32 3489 32 2829
Fragments arranged by size:
3489 2829 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2278 2495 1316
Size: 962 217 5203
Fragments arranged by size:
5203 962 217
XbaI T'CTAG_A
Cuts at: 3718 3718
Size: 6382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2627 2627
Size: 6382
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6382
XmaI C'CCGG_G
Cuts at: 1870 3707 1870
Size: 1837 4545
XmnI GAAnn'nnTTC
Cuts at: 3266 4511 3266
Size: 1245 5137
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4190 276
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI
NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SanDI SfiI SfoI SrfI SwaI