Restriction map for
pFN21AE1982
(Circular) (Six-base) MAPSORT of: pFN21AE1982.seq Check: 5404 from: 1 to: 6379
pFN21AE1982 6379 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3722 1937
Size: 1785 4594
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3231 3353 4442 5826 6094 1632
Size: 1599 122 1089 1384 268 1917
Fragments arranged by size:
1917 1599 1384 1089 268 122
AfeI AGC'GCT
Cuts at: 2028 2028
Size: 6379
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 5278 6255 1203
Size: 4075 977 1327
Fragments arranged by size:
4075 1327 977
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 2468 2763 1166
Size: 1302 295 4782
Fragments arranged by size:
4782 1302 295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2363 2681 1184
Size: 1179 318 4882
Fragments arranged by size:
4882 1179 318
AlwNI CAG_nnn'CTG
Cuts at: 3242 4122 5694 3242
Size: 880 1572 3927
Fragments arranged by size:
3927 1572 880
ApaI G_GGCC'C
Cuts at: 2052 2104 2416 2052
Size: 52 312 6015
Fragments arranged by size:
6015 312 52
ApaLI G'TGCA_C
Cuts at: 3521 4436 5592 3521
Size: 915 1156 4308
Fragments arranged by size:
4308 1156 915
ApoI r'AATT_y
Cuts at: 1493 2848 3434 3688 3914 4066 6175 1493
Size: 1355 586 254 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 586 254 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2751 4933 160
Size: 872 1719 2182 1606
Fragments arranged by size:
2182 1719 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 2103 2277 3704 1870
Size: 73 97 63 174 1427 4545
Fragments arranged by size:
4545 1427 174 97 73 63
BamHI G'GATC_C
Cuts at: 3709 4076 3709
Size: 367 6012
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2154 2234 3215 3700 5155
Size: 325 217 627 367 80 981 485 1455
Cuts at: 5155 618
Size: 1842
Fragments arranged by size:
1842 1455 981 627 485 367 325 217
80
BanII G_rGCy'C
Cuts at: 1391 2052 2066 2104 2416 3698 1391
Size: 661 14 38 312 1282 4072
Fragments arranged by size:
4072 1282 661 312 38 14
BbeI G_GCGC'C
Cuts at: 2158 2238 2158
Size: 80 6299
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2824 3057 3634 928
Size: 971 925 233 577 3673
Fragments arranged by size:
3673 971 925 577 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2462 4271 5481 2462
Size: 1809 1210 3360
Fragments arranged by size:
3360 1809 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2081 4990 136
Size: 107 122 71 631 605 409 2909 1525
Fragments arranged by size:
2909 1525 631 605 409 122 107 71
BglII A'GATC_T
Cuts at: 3249 3283 6374 3249
Size: 34 3091 3254
Fragments arranged by size:
3254 3091 34
BlpI GC'TnA_GC
Cuts at: 3796 3796
Size: 6379
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2104 2416 2742 3525 4440 5596 1790
Size: 262 52 312 326 783 915 1156 2573
Fragments arranged by size:
2573 1156 915 783 326 312 262 52
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4110 5068 442
Size: 416 53 83 399 24 2693 958 1753
Fragments arranged by size:
2693 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2633 1056
Size: 1577 4802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2658 3219 3278 3321 5039 1239
Size: 1419 561 59 43 1718 2579
Fragments arranged by size:
2579 1718 1419 561 59 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4504 5369 5667 4504
Size: 865 298 5216
Fragments arranged by size:
5216 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5042 882
Size: 617 3543 2219
Fragments arranged by size:
3543 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4170 493
Size: 711 2966 2702
Fragments arranged by size:
2966 2702 711
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2069 2155 2235 4186
Size: 53 83 186 936 536 86 80 1951
Cuts at: 4186 4568 5203 275
Size: 382 635 1451
Fragments arranged by size:
1951 1451 936 635 536 382 186 86
83 80 53
BsaWI w'CCGG_w
Cuts at: 4812 5210 5484 5631 6042 4812
Size: 398 274 147 411 5149
Fragments arranged by size:
5149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 3297 3327 1178
Size: 30 165 30 966 30 898 30 4230
Fragments arranged by size:
4230 966 898 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2303 3318 1186
Size: 1117 1015 4247
Fragments arranged by size:
4247 1117 1015
BseYI C'CCAG_C
Cuts at: 2092 2097 2415 3145 5582 6078 2092
Size: 5 318 730 2437 496 2393
Fragments arranged by size:
2437 2393 730 496 318 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2541 1163
Size: 573 805 5001
Fragments arranged by size:
5001 805 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2085 2182 2230 3829 4590 5618
Size: 275 186 84 97 48 1599 761 1028
Cuts at: 5618 1540
Size: 2301
Fragments arranged by size:
2301 1599 1028 761 275 186 97 84
48
BsiHKAI G_wGCw'C
Cuts at: 2687 3525 4440 4525 5596 2687
Size: 838 915 85 1071 3470
Fragments arranged by size:
3470 1071 915 838 85
BsmI GAATG_Cn'
Cuts at: 3301 3890 3983 3301
Size: 589 93 5697
Fragments arranged by size:
5697 589 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2066 2104 2416 2687 2742 3525
Size: 399 262 14 38 312 271 55 783
Cuts at: 3525 3698 4440 4525 5596 1391
Size: 173 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 399 312 271 262
173 85 55 38 14
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3734 844
Size: 2890 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2695 4266 1394
Size: 429 872 1571 3507
Fragments arranged by size:
3507 1571 872 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3204 4874 5048 65
Size: 1183 1956 1670 174 1396
Fragments arranged by size:
1956 1670 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5023 5210 1700
Size: 110 3213 187 2869
Fragments arranged by size:
3213 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3651 96
Size: 3555 2824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2439 3568 794
Size: 1645 1129 3605
Fragments arranged by size:
3605 1645 1129
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2245
Size: 6379
BstYI r'GATC_y
Cuts at: 1972 3249 3283 3709 4076 4461 4478 5930 6016
Size: 1277 34 426 367 385 17 1452 86
Cuts at: 6016 6028 6374 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1277 426 385 367 346 86
34 17 12
Bsu36I CC'TnA_GG
Cuts at: 2270 2270
Size: 6379
BtgI C'CryG_G
Cuts at: 513 1065 2622 2800 2824 2836 3471 513
Size: 552 1557 178 24 12 635 3421
Fragments arranged by size:
3421 1557 635 552 178 24 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 2529 3895 4689 4709 2529
Size: 1366 794 20 4199
Fragments arranged by size:
4199 1366 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6344
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2082 2145 2175
Size: 54 1425 50 275 18 252 63 30
Cuts at: 2175 2821 3358 3826 4715 6344 8
Size: 646 537 468 889 1629 43
Fragments arranged by size:
1629 1425 889 646 537 468 275 252
63 54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2082 3826 1537
Size: 275 270 1744 4090
Fragments arranged by size:
4090 1744 275 270
EarI CTCTTCn'nnn_
Cuts at: 1353 4309 1353
Size: 2956 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2062 4966 5340 5486 387
Size: 1064 138 473 2904 374 146 1280
Fragments arranged by size:
2904 1280 1064 473 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2658 3219 3231 3278 3321 3353 4442
Size: 393 1026 561 12 47 43 32 1089
Cuts at: 4442 5039 5826 6094 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1089 1026 787 597 561 393 268
47 43 32 12
EcoO109I rG'GnC_Cy
Cuts at: 2266 2274 2266
Size: 8 6371
EcoRI G'AATT_C
Cuts at: 3688 4066 3688
Size: 378 6001
EcoRV GAT'ATC
Cuts at: 3495 3495
Size: 6379
FspI TGC'GCA
Cuts at: 3572 4885 3572
Size: 1313 5066
FspAI rTGC'GCAy
Cuts at: 3572 3572
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 2030 2158 2238 5526 1678
Size: 352 128 80 3288 2531
Fragments arranged by size:
3288 2531 352 128 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 3479 3511 5020
Size: 32 135 32 797 32 1078 32 1509
Cuts at: 5020 5052 5094 5126 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1509 1078 797 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3723 3969 669
Size: 988 281 1230 555 246 3079
Fragments arranged by size:
3079 1230 988 555 281 246
HindIII A'AGCT_T
Cuts at: 748 2490 2862 748
Size: 1742 372 4265
Fragments arranged by size:
4265 1742 372
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KasI G'GCGC_C
Cuts at: 2154 2234 2154
Size: 80 6299
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3201 5493 5677 1305
Size: 1896 2292 184 2007
Fragments arranged by size:
2292 2007 1896 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4557
Fragments arranged by size:
4557 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 2515 2663 2763 3578 4337 4696
Size: 648 1302 47 148 100 815 759 359
Cuts at: 4696 4855 518
Size: 159 2042
Fragments arranged by size:
2042 1302 815 759 648 359 159 148
100 47
MspA1I CmG'CkG
Cuts at: 1606 2054 3149 3614 3795 4472 5620 5865 1606
Size: 448 1095 465 181 677 1148 245 2120
Fragments arranged by size:
2120 1148 1095 677 465 448 245 181
NarI GG'CG_CC
Cuts at: 2155 2235 2155
Size: 80 6299
NcoI C'CATG_G
Cuts at: 513 1065 2622 2800 2836 513
Size: 552 1557 178 36 4056
Fragments arranged by size:
4056 1557 552 178 36
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6379
NheI G'CTAG_C
Cuts at: 1052 2629 1052
Size: 1577 4802
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 3737 5282 1132
Size: 97 2508 1545 2229
Fragments arranged by size:
2508 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2059 1348
Size: 711 5668
PciI A'CATG_T
Cuts at: 5278 5278
Size: 6379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2900 1108
Size: 1792 4587
PfoI T'CCnGG_A
Cuts at: 2705 3255 2705
Size: 550 5829
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 5961 5993 4448
Size: 32 1481 32 4834
Fragments arranged by size:
4834 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2266 2274 2266
Size: 8 6371
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6379
PsiI TTA'TAA
Cuts at: 3949 3949
Size: 6379
PspOMI G'GGCC_C
Cuts at: 2048 2100 2412 2048
Size: 52 312 6015
Fragments arranged by size:
6015 312 52
PstI C_TGCA'G
Cuts at: 830 3731 830
Size: 2901 3478
PvuI CG_AT'CG
Cuts at: 2001 2230 2001
Size: 229 6150
PvuII CAG'CTG
Cuts at: 3149 3614 3149
Size: 465 5914
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 4627 6339 4627
Size: 1712 4667
SfcI C'TryA_G
Cuts at: 826 1045 2161 3727 4862 5543 5734 826
Size: 219 1116 1566 1135 681 191 1471
Fragments arranged by size:
1566 1471 1135 1116 681 219 191
SfoI GGC'GCC
Cuts at: 2156 2236 2156
Size: 80 6299
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 2042 2105 2279 3706 1872
Size: 170 63 174 1427 4545
Fragments arranged by size:
4545 1427 174 170 63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4483 5384 5646 820
Size: 197 926 2540 901 262 1553
Fragments arranged by size:
2540 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6379
StyI C'CwwG_G
Cuts at: 513 1065 2622 2788 2800 2836 3090 3818 513
Size: 552 1557 166 12 36 254 728 3074
Fragments arranged by size:
3074 1557 728 552 254 166 36 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3199 4406 4423 4576 4761 5206 325
Size: 1255 1619 1207 17 153 185 445 1498
Fragments arranged by size:
1619 1498 1255 1207 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3651 4625 6337
Size: 274 80 33 51 1443 1674 974 1712
Cuts at: 6337 96
Size: 138
Fragments arranged by size:
1712 1674 1443 974 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3484 3516 6339 6371 3484
Size: 32 2823 32 3492
Fragments arranged by size:
3492 2823 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 2040 2103 2277 3704 1870
Size: 170 63 174 1427 4545
Fragments arranged by size:
4545 1427 174 170 63
XmnI GAAnn'nnTTC
Cuts at: 3641 4508 3641
Size: 867 5512
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI
FseI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI XcmI