Restriction map for
pFN21AE1980
(Circular) (Six-base) MAPSORT of: pFN21AE1980.seq Check: 1789 from: 1 to: 6376
pFN21AE1980 6376 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2727 3209 3719 1937
Size: 790 482 510 4594
Fragments arranged by size:
4594 790 510 482
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2095 3302 3558 4439 5823 6091 1632
Size: 463 1207 256 881 1384 268 1917
Fragments arranged by size:
1917 1384 1207 881 463 268 256
AflII C'TTAA_G
Cuts at: 820 1017 2897 820
Size: 197 1880 4299
Fragments arranged by size:
4299 1880 197
AflIII A'CryG_T
Cuts at: 1203 2633 5275 6252 1203
Size: 1430 2642 977 1327
Fragments arranged by size:
2642 1430 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AlwNI CAG_nnn'CTG
Cuts at: 4119 5691 4119
Size: 1572 4804
ApaI G_GGCC'C
Cuts at: 3435 3435
Size: 6376
ApaLI G'TGCA_C
Cuts at: 4433 5589 4433
Size: 1156 5220
ApoI r'AATT_y
Cuts at: 1493 2456 2624 3685 3911 4063 6172 1493
Size: 963 168 1061 226 152 2109 1697
Fragments arranged by size:
2109 1697 1061 963 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 4930 160
Size: 872 3898 1606
Fragments arranged by size:
3898 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 3003 3701 1870
Size: 73 1060 698 4545
Fragments arranged by size:
4545 1060 698 73
BamHI G'GATC_C
Cuts at: 2605 3706 4073 2605
Size: 1101 367 4908
Fragments arranged by size:
4908 1101 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3697 5152 618
Size: 325 217 627 1910 1455 1842
Fragments arranged by size:
1910 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3183 3435 3695 1391
Size: 1792 252 260 4072
Fragments arranged by size:
4072 1792 260 252
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 2214 2440 2970 3300 928
Size: 971 213 102 226 530 330 4004
Fragments arranged by size:
4004 971 530 330 226 213 102
BbvCI CC'TCA_GC
Cuts at: 2403 2403
Size: 6376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4567 4601 1903
Size: 34 50 34 2546 34 3678
Fragments arranged by size:
3678 2546 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 3222 1548
Size: 1674 4702
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4987 136
Size: 107 122 71 631 605 3315 1525
Fragments arranged by size:
3315 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6371 6371
Size: 6376
BlpI GC'TnA_GC
Cuts at: 3793 3793
Size: 6376
Bme1580I G_kGCm'C
Cuts at: 1790 3435 4437 5593 1790
Size: 1645 1002 1156 2573
Fragments arranged by size:
2573 1645 1156 1002
BmgBI CAC'GTC
Cuts at: 1318 2171 2636 1318
Size: 853 465 5058
Fragments arranged by size:
5058 853 465
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4107 5065 442
Size: 416 53 83 399 24 2690 958 1753
Fragments arranged by size:
2690 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2291 1056
Size: 1235 5141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5036 1239
Size: 3797 2579
Bpu10I CC'TnA_GC
Cuts at: 2403 2403
Size: 6376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4501 5366 5664 4501
Size: 865 298 5213
Fragments arranged by size:
5213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3054 5039 882
Size: 617 1555 1985 2219
Fragments arranged by size:
2219 1985 1555 617
BsaAI yAC'GTr
Cuts at: 493 1204 2980 4167 493
Size: 711 1776 1187 2702
Fragments arranged by size:
2702 1776 1187 711
BsaBI GATnn'nnATC
Cuts at: 2604 6370 2604
Size: 3766 2610
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4183 4565 5200 275
Size: 53 83 186 936 2650 382 635 1451
Fragments arranged by size:
2650 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4809 5207 5481 5628 6039 4809
Size: 398 274 147 411 5146
Fragments arranged by size:
5146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2969 2999 1178
Size: 30 165 30 1566 30 4555
Fragments arranged by size:
4555 1566 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3404 1186
Size: 2218 4158
BseYI C'CCAG_C
Cuts at: 2212 3248 3261 3307 3352 5579 6075 2212
Size: 1036 13 46 45 2227 496 2513
Fragments arranged by size:
2513 2227 1036 496 46 45 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2124 2136 2202 1163
Size: 573 388 12 66 5337
Fragments arranged by size:
5337 573 388 66 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3826 4587 5615 1540
Size: 275 186 1825 761 1028 2301
Fragments arranged by size:
2301 1825 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4437 4522 5593 4437
Size: 85 1071 5220
Fragments arranged by size:
5220 1071 85
BsmI GAATG_Cn'
Cuts at: 2664 3512 3887 3980 2664
Size: 848 375 93 5060
Fragments arranged by size:
5060 848 375 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3183 3435 3695 4437 4522 5593 1391
Size: 399 1393 252 260 742 85 1071 2174
Fragments arranged by size:
2174 1393 1071 742 399 260 252 85
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 2124 4265 5995 2124
Size: 2141 1730 2505
Fragments arranged by size:
2505 2141 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3731 844
Size: 2887 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2686 3016 3112 4263 1394
Size: 429 863 330 96 1151 3507
Fragments arranged by size:
3507 1151 863 429 330 96
BsrDI GCAATG_nn'
Cuts at: 65 1248 2653 4871 5045 65
Size: 1183 1405 2218 174 1396
Fragments arranged by size:
2218 1405 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5020 5207 1700
Size: 110 3210 187 2869
Fragments arranged by size:
3210 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6376
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6376
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2461 2041
Size: 420 5956
BstYI r'GATC_y
Cuts at: 1972 2605 2856 3706 4073 4458 4475 5927 6013
Size: 633 251 850 367 385 17 1452 86
Cuts at: 6013 6025 6371 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 850 633 385 367 346 251
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2870 2940 3436 2870
Size: 70 496 5810
Fragments arranged by size:
5810 496 70
BtgI C'CryG_G
Cuts at: 513 1065 2799 3183 513
Size: 552 1734 384 3706
Fragments arranged by size:
3706 1734 552 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5637
Fragments arranged by size:
5637 683 56
BtsI GCAGTG_nn'
Cuts at: 2397 3892 4686 4706 2397
Size: 1495 794 20 4067
Fragments arranged by size:
4067 1495 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6341
DraI TTT'AAA
Cuts at: 2629 3680 2629
Size: 1051 5325
DraIII CAC_nnn'GTG
Cuts at: 2062 2062
Size: 6376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 3823 4712
Size: 54 1425 50 275 18 223 1770 889
Cuts at: 4712 6341 8
Size: 1629 43
Fragments arranged by size:
1770 1629 1425 889 275 223 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3823 1537
Size: 275 2011 4090
Fragments arranged by size:
4090 2011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2423 2553 2919 3123 3134 3620 4306 1353
Size: 1070 130 366 204 11 486 686 3423
Fragments arranged by size:
3423 1070 686 486 366 204 130 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2423 3559 4963 5337 5483 387
Size: 1064 138 834 1136 1404 374 146 1280
Fragments arranged by size:
1404 1280 1136 1064 834 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2095 3302 3558 4439 5036 5823 6091
Size: 393 463 1207 256 881 597 787 268
Cuts at: 6091 1239
Size: 1524
Fragments arranged by size:
1524 1207 881 787 597 463 393 268
256
EcoNI CCTnn'n_nnAGG
Cuts at: 2873 2873
Size: 6376
EcoO109I rG'GnC_Cy
Cuts at: 3200 3431 3432 3200
Size: 231 1 6144
Fragments arranged by size:
6144 231 1
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
EcoRV GAT'ATC
Cuts at: 2758 3026 2758
Size: 268 6108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3451 3602 3451
Size: 151 6225
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 3559 5523 1678
Size: 1881 1964 2531
Fragments arranged by size:
2531 1964 1881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2427 2459 5017
Size: 32 135 32 488 32 335 32 2558
Cuts at: 5017 5049 5091 5123 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2558 488 335 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3193 3720 3966 669
Size: 988 281 1255 527 246 3079
Fragments arranged by size:
3079 1255 988 527 281 246
HindIII A'AGCT_T
Cuts at: 748 2894 3495 748
Size: 2146 601 3629
Fragments arranged by size:
3629 2146 601
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 3975 3975
Size: 6376
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 3354 5490 5674 1305
Size: 753 1296 2136 184 2007
Fragments arranged by size:
2136 2007 1296 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2055 10
Size: 54 1425 343 223 4331
Fragments arranged by size:
4331 1425 343 223 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3374 4334 4693 4852 518
Size: 648 2208 960 359 159 2042
Fragments arranged by size:
2208 2042 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2216 2420 3307 3792 4469 5617 5862 1606
Size: 610 204 887 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 677 610 485 245 204
NcoI C'CATG_G
Cuts at: 513 1065 2799 3183 513
Size: 552 1734 384 3706
Fragments arranged by size:
3706 1734 552 384
NdeI CA'TA_TG
Cuts at: 387 2680 387
Size: 2293 4083
NheI G'CTAG_C
Cuts at: 1052 2287 1052
Size: 1235 5141
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 3328 3734 5279 1132
Size: 97 2099 406 1545 2229
Fragments arranged by size:
2229 2099 1545 406 97
PasI CC'CwG_GG
Cuts at: 1348 2133 1348
Size: 785 5591
PciI A'CATG_T
Cuts at: 5275 5275
Size: 6376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2062 1108
Size: 954 5422
PfoI T'CCnGG_A
Cuts at: 2445 2445
Size: 6376
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3200 3200
Size: 6376
PshAI GACnn'nnGTC
Cuts at: 1613 3205 1613
Size: 1592 4784
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PspOMI G'GGCC_C
Cuts at: 3431 3431
Size: 6376
PstI C_TGCA'G
Cuts at: 830 2425 3728 830
Size: 1595 1303 3478
Fragments arranged by size:
3478 1595 1303
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 2216 2420 3307 2216
Size: 204 887 5285
Fragments arranged by size:
5285 887 204
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SfcI C'TryA_G
Cuts at: 826 1045 2380 2421 3724 4859 5540 5731 826
Size: 219 1335 41 1303 1135 681 191 1471
Fragments arranged by size:
1471 1335 1303 1135 681 219 191 41
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 3703 1872
Size: 1831 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2897 4480 5381 5643 820
Size: 197 926 954 1583 901 262 1553
Fragments arranged by size:
1583 1553 954 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2980 4167 493
Size: 2487 1187 2702
Fragments arranged by size:
2702 2487 1187
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SspI AAT'ATT
Cuts at: 5 52 2956 4300 5
Size: 47 2904 1344 2081
Fragments arranged by size:
2904 2081 1344 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6376
StyI C'CwwG_G
Cuts at: 513 1065 2186 2313 2799 3183 3213 3504 3815
Size: 552 1121 127 486 384 30 291 311
Cuts at: 3815 513
Size: 3074
Fragments arranged by size:
3074 1121 552 486 384 311 291 127
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 2498 3084 3132 4403 4420 4573
Size: 1255 873 45 586 48 1271 17 153
Cuts at: 4573 4758 5203 325
Size: 185 445 1498
Fragments arranged by size:
1498 1271 1255 873 586 445 185 153
48 45 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2193 4622 6334
Size: 274 80 33 51 1443 216 2429 1712
Cuts at: 6334 96
Size: 138
Fragments arranged by size:
2429 1712 1443 274 216 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6336 6368 6336
Size: 32 6344
Tth111I GACn'n_nGTC
Cuts at: 1316 2834 1316
Size: 1518 4858
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 3701 1870
Size: 1831 4545
XmnI GAAnn'nnTTC
Cuts at: 4505 4505
Size: 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI