Restriction map for
pFN21AE1979
(Circular) (Six-base) MAPSORT of: pFN21AE1979.seq Check: 9843 from: 1 to: 6376
pFN21AE1979 6376 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3719 1937
Size: 1782 4594
Acc65I G'GTAC_C
Cuts at: 2376 3697 2376
Size: 1321 5055
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 2673 3274 4439 5823 6091 1632
Size: 743 298 601 1165 1384 268 1917
Fragments arranged by size:
1917 1384 1165 743 601 298 268
AfeI AGC'GCT
Cuts at: 2129 2129
Size: 6376
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 5275 6252 1203
Size: 4072 977 1327
Fragments arranged by size:
4072 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6376
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2493 1184
Size: 1309 5067
AlwNI CAG_nnn'CTG
Cuts at: 3315 4119 5691 3315
Size: 804 1572 4000
Fragments arranged by size:
4000 1572 804
ApaLI G'TGCA_C
Cuts at: 4433 5589 4433
Size: 1156 5220
ApoI r'AATT_y
Cuts at: 1493 2056 3020 3685 3911 4063 6172 1493
Size: 563 964 665 226 152 2109 1697
Fragments arranged by size:
2109 1697 964 665 563 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3144 4930 160
Size: 872 2112 1786 1606
Fragments arranged by size:
2112 1786 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 3701 1870
Size: 73 1758 4545
Fragments arranged by size:
4545 1758 73
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2187 2376 3697 5152 618
Size: 325 217 627 400 189 1321 1455 1842
Fragments arranged by size:
1842 1455 1321 627 400 325 217 189
BanII G_rGCy'C
Cuts at: 1391 2091 3695 1391
Size: 700 1604 4072
Fragments arranged by size:
4072 1604 700
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4567 4601 1903
Size: 34 2630 34 3678
Fragments arranged by size:
3678 2630 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 3091 1548
Size: 1543 4833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3318 3439 4987
Size: 107 122 71 631 605 1646 121 1548
Cuts at: 4987 136
Size: 1525
Fragments arranged by size:
1646 1548 1525 631 605 122 121 107
71
BglII A'GATC_T
Cuts at: 6371 6371
Size: 6376
BlpI GC'TnA_GC
Cuts at: 2658 3793 2658
Size: 1135 5241
Bme1580I G_kGCm'C
Cuts at: 1790 2190 3276 4437 5593 1790
Size: 400 1086 1161 1156 2573
Fragments arranged by size:
2573 1161 1156 1086 400
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2085 2573 4107
Size: 416 53 83 399 24 668 488 1534
Cuts at: 4107 5065 442
Size: 958 1753
Fragments arranged by size:
1753 1534 958 668 488 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2849 1056
Size: 1793 4583
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2330 3433 2330
Size: 1103 5273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3215 3686 5036 1239
Size: 1976 471 1350 2579
Fragments arranged by size:
2579 1976 1350 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2961 4501 5366 5664 2961
Size: 1540 865 298 3673
Fragments arranged by size:
3673 1540 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3025 3488 5039 882
Size: 617 1526 463 1551 2219
Fragments arranged by size:
2219 1551 1526 617 463
BsaAI yAC'GTr
Cuts at: 493 1204 4167 493
Size: 711 2963 2702
Fragments arranged by size:
2963 2702 711
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4183 4565 5200 275
Size: 53 83 186 936 2650 382 635 1451
Fragments arranged by size:
2650 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4809 5207 5481 5628 6039 4809
Size: 398 274 147 411 5146
Fragments arranged by size:
5146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6151
Fragments arranged by size:
6151 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3420 1186
Size: 2234 4142
BseYI C'CCAG_C
Cuts at: 5579 6075 5579
Size: 496 5880
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2435 1163
Size: 573 699 5104
Fragments arranged by size:
5104 699 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3826 4587 5615 1540
Size: 275 186 1825 761 1028 2301
Fragments arranged by size:
2301 1825 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2751 4437 4522 5593 2751
Size: 1686 85 1071 3534
Fragments arranged by size:
3534 1686 1071 85
BsmI GAATG_Cn'
Cuts at: 2731 3604 3887 3980 2731
Size: 873 283 93 5127
Fragments arranged by size:
5127 873 283 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2190 2751 3276 3695 4437 4522
Size: 399 301 99 561 525 419 742 85
Cuts at: 4522 5593 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 561 525 419 399 301
99 85
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 2551 4265 5995 2551
Size: 1714 1730 2932
Fragments arranged by size:
2932 1730 1714
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2724 3731 844
Size: 1880 1007 3489
Fragments arranged by size:
3489 1880 1007
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 4263 1394
Size: 429 293 2147 3507
Fragments arranged by size:
3507 2147 429 293
BsrDI GCAATG_nn'
Cuts at: 65 1248 2344 3552 4871 5045 65
Size: 1183 1096 1208 1319 174 1396
Fragments arranged by size:
1396 1319 1208 1183 1096 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2373 3324 5020 5207 1700
Size: 110 563 951 1696 187 2869
Fragments arranged by size:
2869 1696 951 563 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6376
BstBI TT'CG_AA
Cuts at: 2494 5180 2494
Size: 2686 3690
BstEII G'GTnAC_C
Cuts at: 1184 3448 1184
Size: 2264 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 2077 2077
Size: 6376
BstYI r'GATC_y
Cuts at: 1972 3670 3706 4073 4458 4475 5927 6013 6025
Size: 1698 36 367 385 17 1452 86 12
Cuts at: 6025 6371 1972
Size: 346 1977
Fragments arranged by size:
1977 1698 1452 385 367 346 86 36
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3648 513
Size: 552 939 1644 3241
Fragments arranged by size:
3241 1644 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5637
Fragments arranged by size:
5637 683 56
BtsI GCAGTG_nn'
Cuts at: 2203 2402 2423 3434 3892 4686 4706 2203
Size: 199 21 1011 458 794 20 3873
Fragments arranged by size:
3873 1011 794 458 199 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6341
DraI TTT'AAA
Cuts at: 2287 3187 3529 3680 2287
Size: 900 342 151 4983
Fragments arranged by size:
4983 900 342 151
DraIII CAC_nnn'GTG
Cuts at: 2155 3280 2155
Size: 1125 5251
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3431 3645 3823
Size: 54 1425 50 275 18 1601 214 178
Cuts at: 3823 4712 6341 8
Size: 889 1629 43
Fragments arranged by size:
1629 1601 1425 889 275 214 178 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3823 1537
Size: 275 2011 4090
Fragments arranged by size:
4090 2011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 2118 3108 3171 3248 4306 1353
Size: 660 105 990 63 77 1058 3423
Fragments arranged by size:
3423 1058 990 660 105 77 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 4963 5337 5483 387
Size: 1064 138 450 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 450 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2375 2673 3215 3274 3686 4439 5036
Size: 393 743 298 542 59 412 753 597
Cuts at: 5036 5823 6091 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 753 743 597 542 412 393
298 268 59
EcoRI G'AATT_C
Cuts at: 2056 3685 4063 2056
Size: 1629 378 4369
Fragments arranged by size:
4369 1629 378
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 2131 5523 1678
Size: 453 3392 2531
Fragments arranged by size:
3392 2531 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5017 5049 5091 5123 1373
Size: 32 135 32 3445 32 42 32 2626
Fragments arranged by size:
3445 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3004 3720 3966 669
Size: 988 281 1066 716 246 3079
Fragments arranged by size:
3079 1066 988 716 281 246
HindIII A'AGCT_T
Cuts at: 748 2914 748
Size: 2166 4210
HpaI GTT'AAC
Cuts at: 3004 3966 3004
Size: 962 5414
KpnI G_GTAC'C
Cuts at: 2380 3701 2380
Size: 1321 5055
MfeI C'AATT_G
Cuts at: 2293 2596 3975 2293
Size: 303 1379 4694
Fragments arranged by size:
4694 1379 303
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 5490 5674 1305
Size: 795 3390 184 2007
Fragments arranged by size:
3390 2007 795 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3433 10
Size: 54 1425 343 1601 2953
Fragments arranged by size:
2953 1601 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2475 4334 4693 4852 518
Size: 648 1309 1859 359 159 2042
Fragments arranged by size:
2042 1859 1309 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3163 3315 3792 4469 5617 5862 1606
Size: 1557 152 477 677 1148 245 2120
Fragments arranged by size:
2120 1557 1148 677 477 245 152
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4885
Fragments arranged by size:
4885 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NheI G'CTAG_C
Cuts at: 1052 2845 1052
Size: 1793 4583
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 2755 3734 5279 1132
Size: 97 1526 979 1545 2229
Fragments arranged by size:
2229 1545 1526 979 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6376
PciI A'CATG_T
Cuts at: 5275 5275
Size: 6376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2110 1108
Size: 1002 5374
PfoI T'CCnGG_A
Cuts at: 2344 2344
Size: 6376
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PstI C_TGCA'G
Cuts at: 830 2721 3728 830
Size: 1891 1007 3478
Fragments arranged by size:
3478 1891 1007
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 3163 3163
Size: 6376
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SapI GCTCTTCn'nnn_
Cuts at: 2013 2118 3171 2013
Size: 105 1053 5218
Fragments arranged by size:
5218 1053 105
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SfcI C'TryA_G
Cuts at: 826 1045 2250 2543 2717 3724 4859 5540 5731
Size: 219 1205 293 174 1007 1135 681 191
Cuts at: 5731 826
Size: 1471
Fragments arranged by size:
1471 1205 1135 1007 681 293 219 191
174
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 3703 1872
Size: 1831 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2976 4480 5381 5643 820
Size: 197 926 1033 1504 901 262 1553
Fragments arranged by size:
1553 1504 1033 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SspI AAT'ATT
Cuts at: 5 52 3148 4300 5
Size: 47 3096 1152 2081
Fragments arranged by size:
3096 2081 1152 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6376
StyI C'CwwG_G
Cuts at: 513 1065 2004 2076 2358 3815 513
Size: 552 939 72 282 1457 3074
Fragments arranged by size:
3074 1457 939 552 282 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4403 4420 4573 4758 5203 325
Size: 1255 2823 17 153 185 445 1498
Fragments arranged by size:
2823 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4622 6334 96
Size: 274 80 33 51 1443 2645 1712 138
Fragments arranged by size:
2645 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2549 2581 3266 3298 6336 6368 2549
Size: 32 685 32 3038 32 2557
Fragments arranged by size:
3038 2557 685 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6376
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2180 2365 2180
Size: 185 6191
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 3701 1870
Size: 1831 4545
XmnI GAAnn'nnTTC
Cuts at: 4505 4505
Size: 6376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI