Restriction map for
pFN21AE1976
(Circular) (Six-base) MAPSORT of: pFN21AE1976.seq Check: 9891 from: 1 to: 6313
pFN21AE1976 6313 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3656 1937
Size: 1719 4594
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2259 3194 4376 5760 6028 1632
Size: 627 935 1182 1384 268 1917
Fragments arranged by size:
1917 1384 1182 935 627 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 5212 6189 1203
Size: 4009 977 1327
Fragments arranged by size:
4009 1327 977
AgeI A'CCGG_T
Cuts at: 2660 5144 2660
Size: 2484 3829
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2136 3239 1184
Size: 952 1103 4258
Fragments arranged by size:
4258 1103 952
AlwNI CAG_nnn'CTG
Cuts at: 2449 2599 2770 3022 4056 5628 2449
Size: 150 171 252 1034 1572 3134
Fragments arranged by size:
3134 1572 1034 252 171 150
ApaI G_GGCC'C
Cuts at: 3112 3121 3255 3112
Size: 9 134 6170
Fragments arranged by size:
6170 134 9
ApaLI G'TGCA_C
Cuts at: 4370 5526 4370
Size: 1156 5157
ApoI r'AATT_y
Cuts at: 1493 3123 3404 3622 3848 4000 6109 1493
Size: 1630 281 218 226 152 2109 1697
Fragments arranged by size:
2109 1697 1630 281 226 218 152
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 3638 1870
Size: 73 1695 4545
Fragments arranged by size:
4545 1695 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3354 3387 3354
Size: 33 6280
BamHI G'GATC_C
Cuts at: 3643 4010 3643
Size: 367 5946
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3276 3634 5089 618
Size: 325 217 627 1489 358 1455 1842
Fragments arranged by size:
1842 1489 1455 627 358 325 217
BanII G_rGCy'C
Cuts at: 1391 2536 3009 3080 3112 3121 3198 3255 3632
Size: 1145 473 71 32 9 77 57 377
Cuts at: 3632 1391
Size: 4072
Fragments arranged by size:
4072 1145 473 377 77 71 57 32
9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2314 928
Size: 971 415 4927
Fragments arranged by size:
4927 971 415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4205 5415 4205
Size: 1210 5103
BclI T'GATC_A
Cuts at: 1548 2400 1548
Size: 852 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2937 3116 4924
Size: 107 122 71 631 605 1265 179 1808
Cuts at: 4924 136
Size: 1525
Fragments arranged by size:
1808 1525 1265 631 605 179 122 107
71
BglII A'GATC_T
Cuts at: 6308 6308
Size: 6313
BlpI GC'TnA_GC
Cuts at: 2267 3730 2267
Size: 1463 4850
Bme1580I G_kGCm'C
Cuts at: 1790 2867 2890 3112 3121 3255 4374 5530 1790
Size: 1077 23 222 9 134 1119 1156 2573
Fragments arranged by size:
2573 1156 1119 1077 222 134 23 9
BmgBI CAC'GTC
Cuts at: 1318 2950 1318
Size: 1632 4681
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3353 4044 5002
Size: 416 53 83 399 24 1936 691 958
Cuts at: 5002 442
Size: 1753
Fragments arranged by size:
1936 1753 958 691 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2486 4973 1239
Size: 1247 2487 2579
Fragments arranged by size:
2579 2487 1247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4438 5303 5601 4438
Size: 865 298 5150
Fragments arranged by size:
5150 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2365 2576 3573 4976 882
Size: 617 866 211 997 1403 2219
Fragments arranged by size:
2219 1403 997 866 617 211
BsaAI yAC'GTr
Cuts at: 493 1204 2573 4104 493
Size: 711 1369 1531 2702
Fragments arranged by size:
2702 1531 1369 711
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4120 4502 5137 275
Size: 53 83 186 936 2587 382 635 1451
Fragments arranged by size:
2587 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2414 2660 4746 5144 5418 5565 5976 2414
Size: 246 2086 398 274 147 411 2751
Fragments arranged by size:
2751 2086 411 398 274 246 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2864 2894 3164 3194 1178
Size: 30 165 30 1461 30 270 30 4297
Fragments arranged by size:
4297 1461 270 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2200 2483 2564 2969 3086 1186
Size: 1014 283 81 405 117 4413
Fragments arranged by size:
4413 1014 405 283 117 81
BseYI C'CCAG_C
Cuts at: 2624 2723 2742 2763 2779 3248 3259 5516 6012
Size: 99 19 21 16 469 11 2257 496
Cuts at: 6012 2624
Size: 2925
Fragments arranged by size:
2925 2257 496 469 99 21 19 16
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2875 3155 3535 3624 1163
Size: 573 1139 280 380 89 3852
Fragments arranged by size:
3852 1139 573 380 280 89
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2536 2965 3080 3385 4374 4459 5530 2536
Size: 429 115 305 989 85 1071 3319
Fragments arranged by size:
3319 1071 989 429 305 115 85
BsmI GAATG_Cn'
Cuts at: 3824 3917 3824
Size: 93 6220
BsmBI CGTCTCn'nnnn_
Cuts at: 2023 2223 2023
Size: 200 6113
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2536 2867 2890 2965 3009 3080 3112
Size: 399 746 331 23 75 44 71 32
Cuts at: 3112 3121 3198 3255 3385 3632 4374 4459 5530
Size: 9 77 57 130 247 742 85 1071
Cuts at: 5530 1391
Size: 2174
Fragments arranged by size:
2174 1071 746 742 399 331 247 130
85 77 75 71 57 44 32 23
9
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 2046 4202 5932 2046
Size: 2156 1730 2427
Fragments arranged by size:
2427 2156 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3668 844
Size: 2824 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 4200 1394
Size: 429 191 2186 3507
Fragments arranged by size:
3507 2186 429 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 2631 4808 4982 65
Size: 1183 1383 2177 174 1396
Fragments arranged by size:
2177 1396 1383 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2514 2660 4957 5144 1700
Size: 110 704 146 2297 187 2869
Fragments arranged by size:
2869 2297 704 187 146 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 2058 2508 4373 5385 2058
Size: 450 1865 1012 2986
Fragments arranged by size:
2986 1865 1012 450
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2599 2862 794
Size: 1805 263 4245
Fragments arranged by size:
4245 1805 263
BstBI TT'CG_AA
Cuts at: 5117 5117
Size: 6313
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstYI r'GATC_y
Cuts at: 1972 3643 4010 4395 4412 5864 5950 5962 6308
Size: 1671 367 385 17 1452 86 12 346
Cuts at: 6308 1972
Size: 1977
Fragments arranged by size:
1977 1671 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2923 2923
Size: 6313
BtgI C'CryG_G
Cuts at: 513 1065 2067 2300 2811 513
Size: 552 1002 233 511 4015
Fragments arranged by size:
4015 1002 552 511 233
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 2438 3829 4623 4643 2438
Size: 1391 794 20 4108
Fragments arranged by size:
4108 1391 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2646 2681 529
Size: 35 2082 35 4161
Fragments arranged by size:
4161 2082 35 35
DraI TTT'AAA
Cuts at: 3617 3617
Size: 6313
DraIII CAC_nnn'GTG
Cuts at: 2832 3380 2832
Size: 548 5765
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5320 809
Size: 507 4004 1802
Fragments arranged by size:
4004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2451 2512
Size: 54 1425 50 275 18 339 282 61
Cuts at: 2512 2650 2786 3760 4649 6278 8
Size: 138 136 974 889 1629 43
Fragments arranged by size:
1629 1425 974 889 339 282 275 138
136 61 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 4243 1353
Size: 825 2065 3423
Fragments arranged by size:
3423 2065 825
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4900 5274 5420 387
Size: 1064 138 3311 374 146 1280
Fragments arranged by size:
3311 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2534 3078 2534
Size: 544 5769
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2486 3194 4376 4973 5760 6028
Size: 393 627 227 708 1182 597 787 268
Cuts at: 6028 1239
Size: 1524
Fragments arranged by size:
1524 1182 787 708 627 597 393 268
227
EcoNI CCTnn'n_nnAGG
Cuts at: 2468 3227 3284 2468
Size: 759 57 5497
Fragments arranged by size:
5497 759 57
EcoO109I rG'GnC_Cy
Cuts at: 2293 2446 3108 3109 3117 3220 3252 3474 3495
Size: 153 662 1 8 103 32 222 21
Cuts at: 3495 2293
Size: 5111
Fragments arranged by size:
5111 662 222 153 103 32 21 8
1
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 2617 2848 5460 1678
Size: 939 231 2612 2531
Fragments arranged by size:
2612 2531 939 231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3164 3196 4954 4986 5028
Size: 32 135 32 1592 32 1758 32 42
Cuts at: 5028 5060 1373
Size: 32 2626
Fragments arranged by size:
2626 1758 1592 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3034 3657 3903 669
Size: 988 281 1096 623 246 3079
Fragments arranged by size:
3079 1096 988 623 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6313
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3500 5427 5611 1305
Size: 2195 1927 184 2007
Fragments arranged by size:
2195 2007 1927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2171 2453 2652 10
Size: 54 1425 343 339 282 199 3671
Fragments arranged by size:
3671 1425 343 339 282 199 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3431 4271 4630 4789 518
Size: 648 2265 840 359 159 2042
Fragments arranged by size:
2265 2042 840 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2624 2770 3419 3729 4406 5554 5799 1606
Size: 1018 146 649 310 677 1148 245 2120
Fragments arranged by size:
2120 1148 1018 677 649 310 245 146
NaeI GCC'GGC
Cuts at: 2516 2516
Size: 6313
NcoI C'CATG_G
Cuts at: 513 1065 2300 2811 513
Size: 552 1235 511 4015
Fragments arranged by size:
4015 1235 552 511
NdeI CA'TA_TG
Cuts at: 387 2347 387
Size: 1960 4353
NgoMIV G'CCGG_C
Cuts at: 2514 2514
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 2123 2569 3671 5216 1132
Size: 97 894 446 1102 1545 2229
Fragments arranged by size:
2229 1545 1102 894 446 97
PasI CC'CwG_GG
Cuts at: 1348 3009 3022 3066 1348
Size: 1661 13 44 4595
Fragments arranged by size:
4595 1661 44 13
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2770 2899 3022 1108
Size: 1662 129 123 4399
Fragments arranged by size:
4399 1662 129 123
PfoI T'CCnGG_A
Cuts at: 3549 3549
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2446 3474 3495 2293
Size: 153 1028 21 5111
Fragments arranged by size:
5111 1028 153 21
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6313
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PspOMI G'GGCC_C
Cuts at: 3108 3117 3251 3108
Size: 9 134 6170
Fragments arranged by size:
6170 134 9
PstI C_TGCA'G
Cuts at: 830 2379 2445 2526 2592 3231 3665 830
Size: 1549 66 81 66 639 434 3478
Fragments arranged by size:
3478 1549 639 434 81 66 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
PvuII CAG'CTG
Cuts at: 2624 2770 3419 2624
Size: 146 649 5518
Fragments arranged by size:
5518 649 146
SacI G_AGCT'C
Cuts at: 2536 3080 2536
Size: 544 5769
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SbfI CC_TGCA'GG
Cuts at: 3665 3665
Size: 6313
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SexAI A'CCwGG_T
Cuts at: 3376 3376
Size: 6313
SfcI C'TryA_G
Cuts at: 826 1045 2375 2441 2522 2588 3227 3236 3661
Size: 219 1330 66 81 66 639 9 425
Cuts at: 3661 4796 5477 5668 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1330 1135 681 639 425 219 191
81 66 66 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 3116 3116
Size: 6313
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3640 1872
Size: 1768 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4417 5318 5580 820
Size: 197 926 2474 901 262 1553
Fragments arranged by size:
2474 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 2123 3671 1132
Size: 991 1548 3774
Fragments arranged by size:
3774 1548 991
SspI AAT'ATT
Cuts at: 5 52 4237 5
Size: 47 4185 2081
Fragments arranged by size:
4185 2081 47
StuI AGG'CCT
Cuts at: 1517 2166 2201 1517
Size: 649 35 5629
Fragments arranged by size:
5629 649 35
StyI C'CwwG_G
Cuts at: 513 1065 2300 2811 3112 3280 3342 3752 513
Size: 552 1235 511 301 168 62 410 3074
Fragments arranged by size:
3074 1235 552 511 410 301 168 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3555 4340 4357 4510 4695 5140 325
Size: 1255 1975 785 17 153 185 445 1498
Fragments arranged by size:
1975 1498 1255 785 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4559 6271 96
Size: 274 80 33 51 1443 2582 1712 138
Fragments arranged by size:
2582 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2864 2896 6273 6305 2864
Size: 32 3377 32 2872
Fragments arranged by size:
3377 2872 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6313
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2461 3091 3384 2461
Size: 630 293 5390
Fragments arranged by size:
5390 630 293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6313
XmaI C'CCGG_G
Cuts at: 1870 3638 1870
Size: 1768 4545
XmnI GAAnn'nnTTC
Cuts at: 4442 4442
Size: 6313
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SapI SfoI SrfI SwaI