Restriction map for
pFN21AE1973
(Circular) (Six-base) MAPSORT of: pFN21AE1973.seq Check: 5696 from: 1 to: 6328
pFN21AE1973 6328 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3671 1937
Size: 1734 4594
Acc65I G'GTAC_C
Cuts at: 3649 3649
Size: 6328
AclI AA'CG_TT
Cuts at: 1563 4455 4828 1563
Size: 2892 373 3063
Fragments arranged by size:
3063 2892 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3518 4391 5775 6043 1632
Size: 1886 873 1384 268 1917
Fragments arranged by size:
1917 1886 1384 873 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6131
AflIII A'CryG_T
Cuts at: 1203 2197 5227 6204 1203
Size: 994 3030 977 1327
Fragments arranged by size:
3030 1327 994 977
AgeI A'CCGG_T
Cuts at: 5159 5159
Size: 6328
AhdI GACnn_n'nnGTC
Cuts at: 5057 5057
Size: 6328
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3046 3204 1184
Size: 1862 158 4308
Fragments arranged by size:
4308 1862 158
AlwNI CAG_nnn'CTG
Cuts at: 4071 5643 4071
Size: 1572 4756
ApaLI G'TGCA_C
Cuts at: 4385 5541 4385
Size: 1156 5172
ApoI r'AATT_y
Cuts at: 1493 2405 3428 3637 3863 4015 6124 1493
Size: 912 1023 209 226 152 2109 1697
Fragments arranged by size:
2109 1697 1023 912 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 2224 3653 1870
Size: 73 281 1429 4545
Fragments arranged by size:
4545 1429 281 73
BamHI G'GATC_C
Cuts at: 2776 3658 4025 2776
Size: 882 367 5079
Fragments arranged by size:
5079 882 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3649 5104 618
Size: 325 217 627 1862 1455 1842
Fragments arranged by size:
1862 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3647 1391
Size: 2256 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2974 3322 928
Size: 971 1075 348 3934
Fragments arranged by size:
3934 1075 971 348
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4519 4553 1903
Size: 34 2582 34 3678
Fragments arranged by size:
3678 2582 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4220 5430 4220
Size: 1210 5118
BclI T'GATC_A
Cuts at: 1548 2997 1548
Size: 1449 4879
BfrBI ATG'CAT
Cuts at: 3243 3243
Size: 6328
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4939 136
Size: 107 122 71 631 605 3267 1525
Fragments arranged by size:
3267 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6323 6323
Size: 6328
BlpI GC'TnA_GC
Cuts at: 3745 3745
Size: 6328
Bme1580I G_kGCm'C
Cuts at: 1790 4389 5545 1790
Size: 2599 1156 2573
Fragments arranged by size:
2599 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2078 2980 1318
Size: 760 902 4666
Fragments arranged by size:
4666 902 760
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4059 5017 442
Size: 416 53 83 399 24 2642 958 1753
Fragments arranged by size:
2642 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2513 2802 3288 4988 1239
Size: 1274 289 486 1700 2579
Fragments arranged by size:
2579 1700 1274 486 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3517 4453 5318 5616 3517
Size: 936 865 298 4229
Fragments arranged by size:
4229 936 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4991 882
Size: 617 3492 2219
Fragments arranged by size:
3492 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4119 493
Size: 711 2915 2702
Fragments arranged by size:
2915 2702 711
BsaBI GATnn'nnATC
Cuts at: 6322 6322
Size: 6328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4135 4517 5152 275
Size: 53 83 186 936 2602 382 635 1451
Fragments arranged by size:
2602 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2647 3598 4761 5159 5433 5580 5991 2647
Size: 951 1163 398 274 147 411 2984
Fragments arranged by size:
2984 1163 951 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3261 3264 3291 3294 1178
Size: 30 165 30 1858 3 27 3 4212
Fragments arranged by size:
4212 1858 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3191 3285 1186
Size: 2005 94 4229
Fragments arranged by size:
4229 2005 94
BseYI C'CCAG_C
Cuts at: 5531 6027 5531
Size: 496 5832
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5755
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3778 4539 5567 1540
Size: 275 186 1777 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3183 4389 4474 5545 3183
Size: 1206 85 1071 3966
Fragments arranged by size:
3966 1206 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3839 3932 3161
Size: 678 93 5557
Fragments arranged by size:
5557 678 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3585 3585
Size: 6328
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3183 3647 4389 4474 5545 1391
Size: 399 1393 464 742 85 1071 2174
Fragments arranged by size:
2174 1393 1071 742 464 399 85
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 4217 5947 4217
Size: 1730 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3683 844
Size: 2839 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4215 1394
Size: 429 2392 3507
Fragments arranged by size:
3507 2392 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2122 4823 4997 65
Size: 1183 874 2701 174 1396
Fragments arranged by size:
2701 1396 1183 874 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4972 5159 1700
Size: 110 3162 187 2869
Fragments arranged by size:
3162 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6328
BssSI C'ACGA_G
Cuts at: 2128 4388 5400 2128
Size: 2260 1012 3056
Fragments arranged by size:
3056 2260 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6328
BstBI TT'CG_AA
Cuts at: 5132 5132
Size: 6328
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6328
BstXI CCAn_nnnn'nTGG
Cuts at: 3214 3214
Size: 6328
BstYI r'GATC_y
Cuts at: 1972 2285 2776 3658 4025 4410 4427 5879 5965
Size: 313 491 882 367 385 17 1452 86
Cuts at: 5965 5977 6323 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 882 491 385 367 346 313
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 2055 2188 2688 3844 4638 4658 2055
Size: 133 500 1156 794 20 3725
Fragments arranged by size:
3725 1156 794 500 133 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2164 2199 529
Size: 35 1600 35 4658
Fragments arranged by size:
4658 1600 35 35
DraI TTT'AAA
Cuts at: 3632 3632
Size: 6328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5335 809
Size: 507 4019 1802
Fragments arranged by size:
4019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2261 2904 3775
Size: 54 1425 50 275 18 431 643 871
Cuts at: 3775 4664 6293 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 871 643 431 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3775 1537
Size: 275 1963 4090
Fragments arranged by size:
4090 1963 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2727 2787 3091 4258 1353
Size: 1374 60 304 1167 3423
Fragments arranged by size:
3423 1374 1167 304 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4915 5289 5435 387
Size: 1064 138 3326 374 146 1280
Fragments arranged by size:
3326 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2802 3288 3518 4391 4988 5775
Size: 393 881 289 486 230 873 597 787
Cuts at: 5775 6043 1239
Size: 268 1524
Fragments arranged by size:
1524 881 873 787 597 486 393 289
268 230
EcoRI G'AATT_C
Cuts at: 3637 4015 3637
Size: 378 5950
EcoRV GAT'ATC
Cuts at: 2968 2968
Size: 6328
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 5475 1678
Size: 3797 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2270 2302 2982 3014 4969
Size: 32 135 32 698 32 680 32 1955
Cuts at: 4969 5001 5043 5075 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1955 698 680 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2452 3064 3672 3918 669
Size: 988 281 514 612 608 246 3079
Fragments arranged by size:
3079 988 612 608 514 281 246
HindIII A'AGCT_T
Cuts at: 748 2666 2932 3473 748
Size: 1918 266 541 3603
Fragments arranged by size:
3603 1918 541 266
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KpnI G_GTAC'C
Cuts at: 3653 3653
Size: 6328
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3195 5442 5626 1305
Size: 1890 2247 184 2007
Fragments arranged by size:
2247 2007 1890 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2263 2906 10
Size: 54 1425 343 431 643 3432
Fragments arranged by size:
3432 1425 643 431 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2244 2577 2942 4286 4645 4804
Size: 648 924 154 333 365 1344 359 159
Cuts at: 4804 518
Size: 2042
Fragments arranged by size:
2042 1344 924 648 365 359 333 159
154
MspA1I CmG'CkG
Cuts at: 1606 2358 3744 4421 5569 5814 1606
Size: 752 1386 677 1148 245 2120
Fragments arranged by size:
2120 1386 1148 752 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5776
NdeI CA'TA_TG
Cuts at: 387 3127 387
Size: 2740 3588
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NsiI A_TGCA'T
Cuts at: 3245 3245
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 2201 2837 3686 5231 1132
Size: 97 972 636 849 1545 2229
Fragments arranged by size:
2229 1545 972 849 636 97
PasI CC'CwG_GG
Cuts at: 1348 3207 1348
Size: 1859 4469
PciI A'CATG_T
Cuts at: 2197 5227 2197
Size: 3030 3298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2169 1108
Size: 1061 5267
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4397 4429 5910 5942 4397
Size: 32 1481 32 4783
Fragments arranged by size:
4783 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 3528 3898 3528
Size: 370 5958
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 6296
PstI C_TGCA'G
Cuts at: 830 2062 3680 830
Size: 1232 1618 3478
Fragments arranged by size:
3478 1618 1232
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
PvuII CAG'CTG
Cuts at: 2358 2358
Size: 6328
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 4576 6288 4576
Size: 1712 4616
SfcI C'TryA_G
Cuts at: 826 1045 2058 3676 4811 5492 5683 826
Size: 219 1013 1618 1135 681 191 1471
Fragments arranged by size:
1618 1471 1135 1013 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6328
SmaI CCC'GGG
Cuts at: 1872 3655 1872
Size: 1783 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2224 3532 4432 5333 5595 820
Size: 197 926 281 1308 900 901 262 1553
Fragments arranged by size:
1553 1308 926 901 900 281 262 197
SnaBI TAC'GTA
Cuts at: 493 4119 493
Size: 3626 2702
SpeI A'CTAG_T
Cuts at: 152 2381 152
Size: 2229 4099
SphI G_CATG'C
Cuts at: 1132 2837 3686 1132
Size: 1705 849 3774
Fragments arranged by size:
3774 1705 849
SspI AAT'ATT
Cuts at: 5 52 2863 4252 5
Size: 47 2811 1389 2081
Fragments arranged by size:
2811 2081 1389 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6328
StyI C'CwwG_G
Cuts at: 513 1065 2213 3767 513
Size: 552 1148 1554 3074
Fragments arranged by size:
3074 1554 1148 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 4355 4372 4525 4710 5155 325
Size: 1255 446 2329 17 153 185 445 1498
Fragments arranged by size:
2329 1498 1255 446 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2069 4574 6286
Size: 274 80 33 51 1443 92 2505 1712
Cuts at: 6286 96
Size: 138
Fragments arranged by size:
2505 1712 1443 274 138 92 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6288 6320 6288
Size: 32 6296
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2826 2826
Size: 6328
XhoI C'TCGA_G
Cuts at: 1943 2224 1943
Size: 281 6047
XmaI C'CCGG_G
Cuts at: 1870 3653 1870
Size: 1783 4545
XmnI GAAnn'nnTTC
Cuts at: 4457 4457
Size: 6328
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI