Restriction map for
pFN21AE1972
(Circular) (Six-base) MAPSORT of: pFN21AE1972.seq Check: 3780 from: 1 to: 6325
pFN21AE1972 6325 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4135 278
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3441 3668 1937
Size: 1504 227 4594
Fragments arranged by size:
4594 1504 227
Acc65I G'GTAC_C
Cuts at: 3646 3646
Size: 6325
AclI AA'CG_TT
Cuts at: 1563 4452 4825 1563
Size: 2889 373 3063
Fragments arranged by size:
3063 2889 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 4388 5772 6040 1632
Size: 1065 1691 1384 268 1917
Fragments arranged by size:
1917 1691 1384 1065 268
AflII C'TTAA_G
Cuts at: 820 1017 2408 820
Size: 197 1391 4737
Fragments arranged by size:
4737 1391 197
AflIII A'CryG_T
Cuts at: 1203 5224 6201 1203
Size: 4021 977 1327
Fragments arranged by size:
4021 1327 977
AgeI A'CCGG_T
Cuts at: 5156 5156
Size: 6325
AhdI GACnn_n'nnGTC
Cuts at: 5054 5054
Size: 6325
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6325
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6325
AlwNI CAG_nnn'CTG
Cuts at: 2944 4068 5640 2944
Size: 1124 1572 3629
Fragments arranged by size:
3629 1572 1124
ApaLI G'TGCA_C
Cuts at: 4382 5538 4382
Size: 1156 5169
ApoI r'AATT_y
Cuts at: 1493 2238 2357 3634 3860 4012 6121 1493
Size: 745 119 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 745 226 152 119
AseI AT'TA_AT
Cuts at: 160 1032 4879 160
Size: 872 3847 1606
Fragments arranged by size:
3847 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
AvaI C'yCGr_G
Cuts at: 1870 1943 3650 1870
Size: 73 1707 4545
Fragments arranged by size:
4545 1707 73
BamHI G'GATC_C
Cuts at: 2491 3655 4022 2491
Size: 1164 367 4794
Fragments arranged by size:
4794 1164 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3239 3433 3526 3646 5101
Size: 325 217 627 1452 194 93 120 1455
Cuts at: 5101 618
Size: 1842
Fragments arranged by size:
1842 1455 1452 627 325 217 194 120
93
BanII G_rGCy'C
Cuts at: 1391 3644 1391
Size: 2253 4072
BbeI G_GCGC'C
Cuts at: 3437 3437
Size: 6325
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2135 2685 2849 3284 3303 928
Size: 971 236 550 164 435 19 3950
Fragments arranged by size:
3950 971 550 435 236 164 19
BbvCI CC'TCA_GC
Cuts at: 2073 3561 3588 2073
Size: 1488 27 4810
Fragments arranged by size:
4810 1488 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4516 4550 1903
Size: 34 50 34 2495 34 3678
Fragments arranged by size:
3678 2495 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2607 4217 5427 2607
Size: 1610 1210 3505
Fragments arranged by size:
3505 1610 1210
BclI T'GATC_A
Cuts at: 1548 2968 1548
Size: 1420 4905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4936 136
Size: 107 122 71 631 605 3264 1525
Fragments arranged by size:
3264 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2803 6320 2803
Size: 3517 2808
BlpI GC'TnA_GC
Cuts at: 3742 3742
Size: 6325
Bme1580I G_kGCm'C
Cuts at: 1790 4386 5542 1790
Size: 2596 1156 2573
Fragments arranged by size:
2596 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6325
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2089 2955 4056
Size: 416 53 83 399 24 672 866 1101
Cuts at: 4056 5014 442
Size: 958 1753
Fragments arranged by size:
1753 1101 958 866 672 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2724 3263 4985 1239
Size: 1485 539 1722 2579
Fragments arranged by size:
2579 1722 1485 539
Bpu10I CC'TnA_GC
Cuts at: 2073 3561 3588 2073
Size: 1488 27 4810
Fragments arranged by size:
4810 1488 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2540 4450 5315 5613 2540
Size: 1910 865 298 3252
Fragments arranged by size:
3252 1910 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4988 882
Size: 617 3489 2219
Fragments arranged by size:
3489 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4116 493
Size: 711 2912 2702
Fragments arranged by size:
2912 2702 711
BsaBI GATnn'nnATC
Cuts at: 6319 6319
Size: 6325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3434 4132 4514 5149
Size: 53 83 186 936 1901 698 382 635
Cuts at: 5149 275
Size: 1451
Fragments arranged by size:
1901 1451 936 698 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4758 5156 5430 5577 5988 4758
Size: 398 274 147 411 5095
Fragments arranged by size:
5095 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2205 2235 3293 3323 1178
Size: 30 165 30 802 30 1058 30 4180
Fragments arranged by size:
4180 1058 802 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3219 3441 1186
Size: 2033 222 4070
Fragments arranged by size:
4070 2033 222
BseYI C'CCAG_C
Cuts at: 2431 2473 3349 3425 5528 6024 2431
Size: 42 876 76 2103 496 2732
Fragments arranged by size:
2732 2103 876 496 76 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5752
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3775 4536 5564 1540
Size: 275 186 1774 761 1028 2301
Fragments arranged by size:
2301 1774 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4386 4471 5542 4386
Size: 85 1071 5169
Fragments arranged by size:
5169 1071 85
BsmI GAATG_Cn'
Cuts at: 3508 3836 3929 3508
Size: 328 93 5904
Fragments arranged by size:
5904 328 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3644 4386 4471 5542 1391
Size: 399 1854 742 85 1071 2174
Fragments arranged by size:
2174 1854 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5988 5988
Size: 6325
BspHI T'CATG_A
Cuts at: 4214 5944 4214
Size: 1730 4595
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3680 844
Size: 2836 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4212 1394
Size: 429 2389 3507
Fragments arranged by size:
3507 2389 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2400 3012 4820 4994 65
Size: 1183 1152 612 1808 174 1396
Fragments arranged by size:
1808 1396 1183 1152 612 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4969 5156 1700
Size: 110 3159 187 2869
Fragments arranged by size:
3159 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6325
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6325
BssSI C'ACGA_G
Cuts at: 4385 5397 4385
Size: 1012 5313
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6325
BstBI TT'CG_AA
Cuts at: 5129 5129
Size: 6325
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6325
BstYI r'GATC_y
Cuts at: 1972 2176 2491 2803 3136 3655 4022 4407 4424
Size: 204 315 312 333 519 367 385 17
Cuts at: 4424 5876 5962 5974 6320 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 519 385 367 346 333 315
312 204 86 17 12
BstZ17I GTA'TAC
Cuts at: 3442 3442
Size: 6325
Bsu36I CC'TnA_GG
Cuts at: 2133 2133
Size: 6325
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5773
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5586
Fragments arranged by size:
5586 683 56
BtsI GCAGTG_nn'
Cuts at: 2808 3333 3841 4635 4655 2808
Size: 525 508 794 20 4478
Fragments arranged by size:
4478 794 525 508 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6325
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6290
DraI TTT'AAA
Cuts at: 2698 3629 2698
Size: 931 5394
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5332 809
Size: 507 4016 1802
Fragments arranged by size:
4016 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3772 4661 6290
Size: 54 1425 50 275 18 1942 889 1629
Cuts at: 6290 8
Size: 43
Fragments arranged by size:
1942 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3772 1537
Size: 275 1960 4090
Fragments arranged by size:
4090 1960 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2155 2211 4255 1353
Size: 802 56 2044 3423
Fragments arranged by size:
3423 2044 802 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4912 5286 5432 387
Size: 1064 138 3323 374 146 1280
Fragments arranged by size:
3323 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2697 2724 3263 4388 4985 5772 6040
Size: 393 1065 27 539 1125 597 787 268
Cuts at: 6040 1239
Size: 1524
Fragments arranged by size:
1524 1125 1065 787 597 539 393 268
27
EcoO109I rG'GnC_Cy
Cuts at: 2083 3023 3103 3224 3509 2083
Size: 940 80 121 285 4899
Fragments arranged by size:
4899 940 285 121 80
EcoRI G'AATT_C
Cuts at: 3634 4012 3634
Size: 378 5947
EcoRV GAT'ATC
Cuts at: 2098 2098
Size: 6325
FspI TGC'GCA
Cuts at: 4831 4831
Size: 6325
HaeII r_GCGC'y
Cuts at: 1678 3437 5472 1678
Size: 1759 2035 2531
Fragments arranged by size:
2531 2035 1759
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2788 2820 3010 3042 3274
Size: 32 135 32 1216 32 190 32 232
Cuts at: 3274 3293 3306 3325 4966 4998 5040 5072 1373
Size: 19 13 19 1641 32 42 32 2626
Fragments arranged by size:
2626 1641 1216 232 190 135 42 32
32 32 32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2577 3669 3915 669
Size: 988 281 639 1092 246 3079
Fragments arranged by size:
3079 1092 988 639 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6325
HpaI GTT'AAC
Cuts at: 3915 3915
Size: 6325
KasI G'GCGC_C
Cuts at: 3433 3433
Size: 6325
KpnI G_GTAC'C
Cuts at: 3650 3650
Size: 6325
MfeI C'AATT_G
Cuts at: 3924 3924
Size: 6325
MluI A'CGCG_T
Cuts at: 6201 6201
Size: 6325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5439 5623 1305
Size: 4134 184 2007
Fragments arranged by size:
4134 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4503
Fragments arranged by size:
4503 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2631 4283 4642 4801 518
Size: 648 1465 1652 359 159 2042
Fragments arranged by size:
2042 1652 1465 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3307 3741 4418 5566 5811 1606
Size: 1701 434 677 1148 245 2120
Fragments arranged by size:
2120 1701 1148 677 434 245
NarI GG'CG_CC
Cuts at: 3434 3434
Size: 6325
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5773
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6325
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6325
NotI GC'GGCC_GC
Cuts at: 3772 3772
Size: 6325
NspI r_CATG'y
Cuts at: 1132 1229 2220 3683 5228 1132
Size: 97 991 1463 1545 2229
Fragments arranged by size:
2229 1545 1463 991 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6325
PciI A'CATG_T
Cuts at: 5224 5224
Size: 6325
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2438 3328 1108
Size: 1330 890 4105
Fragments arranged by size:
4105 1330 890
PmeI GTTT'AAAC
Cuts at: 3629 3629
Size: 6325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4394 4426 5907 5939 4394
Size: 32 1481 32 4780
Fragments arranged by size:
4780 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3023 3509 3023
Size: 486 5839
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6325
PsiI TTA'TAA
Cuts at: 3895 3895
Size: 6325
PstI C_TGCA'G
Cuts at: 830 2455 2803 3677 830
Size: 1625 348 874 3478
Fragments arranged by size:
3478 1625 874 348
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6325
PvuII CAG'CTG
Cuts at: 3307 3307
Size: 6325
SalI G'TCGA_C
Cuts at: 1936 3667 1936
Size: 1731 4594
SbfI CC_TGCA'GG
Cuts at: 3677 3677
Size: 6325
ScaI AGT'ACT
Cuts at: 4573 6285 4573
Size: 1712 4613
SexAI A'CCwGG_T
Cuts at: 3246 3246
Size: 6325
SfcI C'TryA_G
Cuts at: 826 1045 2451 2799 2936 3286 3673 4808 5489
Size: 219 1406 348 137 350 387 1135 681
Cuts at: 5489 5680 826
Size: 191 1471
Fragments arranged by size:
1471 1406 1135 681 387 350 348 219
191 137
SfoI GGC'GCC
Cuts at: 3435 3435
Size: 6325
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6325
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6325
SmaI CCC'GGG
Cuts at: 1872 3652 1872
Size: 1780 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2408 2555 4429 5330 5592 820
Size: 197 926 465 147 1874 901 262 1553
Fragments arranged by size:
1874 1553 926 901 465 262 197 147
SnaBI TAC'GTA
Cuts at: 493 4116 493
Size: 3623 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6325
SphI G_CATG'C
Cuts at: 1132 3683 1132
Size: 2551 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5
Size: 47 4197 2081
Fragments arranged by size:
4197 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6325
StyI C'CwwG_G
Cuts at: 513 1065 2487 3764 513
Size: 552 1422 1277 3074
Fragments arranged by size:
3074 1422 1277 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2136 4352 4369 4522 4707 5152 325
Size: 1255 556 2216 17 153 185 445 1498
Fragments arranged by size:
2216 1498 1255 556 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2951 4571 6283
Size: 274 80 33 51 1443 974 1620 1712
Cuts at: 6283 96
Size: 138
Fragments arranged by size:
1712 1620 1443 974 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2581 2613 6285 6317 2581
Size: 32 3672 32 2589
Fragments arranged by size:
3672 2589 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6325
XbaI T'CTAG_A
Cuts at: 3661 3661
Size: 6325
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6325
XmaI C'CCGG_G
Cuts at: 1870 3650 1870
Size: 1780 4545
XmnI GAAnn'nnTTC
Cuts at: 3064 4454 3064
Size: 1390 4935
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4133 276
Size: 53 83 186 936 2599 2468
Fragments arranged by size:
2599 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SrfI SwaI XcmI