Restriction map for
pFN21AE1970
(Circular) (Six-base) MAPSORT of: pFN21AE1970.seq Check: 4874 from: 1 to: 6316
pFN21AE1970 6316 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4126 278
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3659 1937
Size: 1722 4594
Acc65I G'GTAC_C
Cuts at: 3637 3637
Size: 6316
AclI AA'CG_TT
Cuts at: 1563 4443 4816 1563
Size: 2880 373 3063
Fragments arranged by size:
3063 2880 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 3459 4379 5763 6031 1632
Size: 788 1039 920 1384 268 1917
Fragments arranged by size:
1917 1384 1039 920 788 268
AflII C'TTAA_G
Cuts at: 820 1017 2894 820
Size: 197 1877 4242
Fragments arranged by size:
4242 1877 197
AflIII A'CryG_T
Cuts at: 1203 2472 3350 3510 5215 6192 1203
Size: 1269 878 160 1705 977 1327
Fragments arranged by size:
1705 1327 1269 977 878 160
AgeI A'CCGG_T
Cuts at: 5147 5147
Size: 6316
AhdI GACnn_n'nnGTC
Cuts at: 3305 5045 3305
Size: 1740 4576
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6316
AlwNI CAG_nnn'CTG
Cuts at: 2086 2873 3344 4059 5631 2086
Size: 787 471 715 1572 2771
Fragments arranged by size:
2771 1572 787 715 471
ApaLI G'TGCA_C
Cuts at: 2320 4373 5529 2320
Size: 2053 1156 3107
Fragments arranged by size:
3107 2053 1156
ApoI r'AATT_y
Cuts at: 1493 2405 2444 3249 3444 3541 3625 3851 4003
Size: 912 39 805 195 97 84 226 152
Cuts at: 4003 6112 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 912 805 226 195 152 97
84 39
AseI AT'TA_AT
Cuts at: 160 1032 4870 160
Size: 872 3838 1606
Fragments arranged by size:
3838 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2045 3641 1870
Size: 73 92 10 1596 4545
Fragments arranged by size:
4545 1596 92 73 10
BamHI G'GATC_C
Cuts at: 3646 4013 3646
Size: 367 5949
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2503 3637 5092 618
Size: 325 217 627 254 462 1134 1455 1842
Fragments arranged by size:
1842 1455 1134 627 462 325 254 217
BanII G_rGCy'C
Cuts at: 1391 3635 1391
Size: 2244 4072
BbeI G_GCGC'C
Cuts at: 2045 2045
Size: 6316
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4507 4541 1903
Size: 34 2570 34 3678
Fragments arranged by size:
3678 2570 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4208 5418 4208
Size: 1210 5106
BclI T'GATC_A
Cuts at: 1548 2569 1548
Size: 1021 5295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2355 4927 136
Size: 107 122 71 631 605 683 2572 1525
Fragments arranged by size:
2572 1525 683 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6311 6311
Size: 6316
BlpI GC'TnA_GC
Cuts at: 2644 3733 2644
Size: 1089 5227
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2324 2508 3394 4377 5533 1790
Size: 421 113 184 886 983 1156 2573
Fragments arranged by size:
2573 1156 983 886 421 184 113
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6316
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3397 4047 5005
Size: 416 53 83 399 24 1980 650 958
Cuts at: 5005 442
Size: 1753
Fragments arranged by size:
1980 1753 958 650 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2566 2566
Size: 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2260 4976 1239
Size: 1021 2716 2579
Fragments arranged by size:
2716 2579 1021
Bpu10I CC'TnA_GC
Cuts at: 2757 2757
Size: 6316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2594 4441 5306 5604 2594
Size: 1847 865 298 3306
Fragments arranged by size:
3306 1847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2063 4979 882
Size: 617 564 2916 2219
Fragments arranged by size:
2916 2219 617 564
BsaAI yAC'GTr
Cuts at: 493 1204 3513 4107 493
Size: 711 2309 594 2702
Fragments arranged by size:
2702 2309 711 594
BsaBI GATnn'nnATC
Cuts at: 2336 6310 2336
Size: 3974 2342
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 2052 2226 4123
Size: 53 83 186 936 509 10 174 1897
Cuts at: 4123 4505 5140 275
Size: 382 635 1451
Fragments arranged by size:
1897 1451 936 635 509 382 186 174
83 53 10
BsaWI w'CCGG_w
Cuts at: 4749 5147 5421 5568 5979 4749
Size: 398 274 147 411 5086
Fragments arranged by size:
5086 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 1178
Size: 30 165 30 855 30 5206
Fragments arranged by size:
5206 855 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6316
BseYI C'CCAG_C
Cuts at: 5519 6015 5519
Size: 496 5820
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5743
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3766 4527 5555 1540
Size: 275 186 1765 761 1028 2301
Fragments arranged by size:
2301 1765 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2324 2610 4377 4462 5533 2324
Size: 286 1767 85 1071 3107
Fragments arranged by size:
3107 1767 1071 286 85
BsmI GAATG_Cn'
Cuts at: 3827 3920 3827
Size: 93 6223
BsmBI CGTCTCn'nnnn_
Cuts at: 2374 2374
Size: 6316
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2324 2508 2610 3394 3635 4377
Size: 399 421 113 184 102 784 241 742
Cuts at: 4377 4462 5533 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 784 742 421 399 241 184
113 102 85
BspEI T'CCGG_A
Cuts at: 5979 5979
Size: 6316
BspHI T'CATG_A
Cuts at: 3013 4205 5935 3013
Size: 1192 1730 3394
Fragments arranged by size:
3394 1730 1192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3218 3671 844
Size: 2374 453 3489
Fragments arranged by size:
3489 2374 453
BsrBI CCG'CTC
Cuts at: 1394 1823 2131 2257 2643 4203 1394
Size: 429 308 126 386 1560 3507
Fragments arranged by size:
3507 1560 429 386 308 126
BsrDI GCAATG_nn'
Cuts at: 65 1248 2802 3048 3219 4811 4985 65
Size: 1183 1554 246 171 1592 174 1396
Fragments arranged by size:
1592 1554 1396 1183 246 174 171
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 4960 5147 1700
Size: 110 291 2859 187 2869
Fragments arranged by size:
2869 2859 291 187 110
BsrGI T'GTAC_A
Cuts at: 96 3333 96
Size: 3237 3079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6316
BssSI C'ACGA_G
Cuts at: 2210 4376 5388 2210
Size: 2166 1012 3138
Fragments arranged by size:
3138 2166 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2086 2215 794
Size: 1292 129 4895
Fragments arranged by size:
4895 1292 129
BstBI TT'CG_AA
Cuts at: 5120 5120
Size: 6316
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6316
BstXI CCAn_nnnn'nTGG
Cuts at: 3535 3535
Size: 6316
BstYI r'GATC_y
Cuts at: 1972 3646 4013 4398 4415 5867 5953 5965 6311
Size: 1674 367 385 17 1452 86 12 346
Cuts at: 6311 1972
Size: 1977
Fragments arranged by size:
1977 1674 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4825
Fragments arranged by size:
4825 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5577
Fragments arranged by size:
5577 683 56
BtsI GCAGTG_nn'
Cuts at: 2703 3078 3607 3832 4626 4646 2703
Size: 375 529 225 794 20 4373
Fragments arranged by size:
4373 794 529 375 225 20
ClaI AT'CG_AT
Cuts at: 1554 2733 1554
Size: 1179 5137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6281
DraI TTT'AAA
Cuts at: 3620 3620
Size: 6316
DraIII CAC_nnn'GTG
Cuts at: 2554 2554
Size: 6316
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5323 809
Size: 507 4007 1802
Fragments arranged by size:
4007 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2420 2946 3763
Size: 54 1425 50 275 18 590 526 817
Cuts at: 3763 4652 6281 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 817 590 526 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3763 1537
Size: 275 1951 4090
Fragments arranged by size:
4090 1951 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2493 2535 2853 3510 3560 4246 1353
Size: 768 372 42 318 657 50 686 3423
Fragments arranged by size:
3423 768 686 657 372 318 50 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4903 5277 5423 387
Size: 1064 138 3314 374 146 1280
Fragments arranged by size:
3314 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2260 2420 3459 4379 4976 5763 6031
Size: 393 628 160 1039 920 597 787 268
Cuts at: 6031 1239
Size: 1524
Fragments arranged by size:
1524 1039 920 787 628 597 393 268
160
EcoNI CCTnn'n_nnAGG
Cuts at: 3594 3594
Size: 6316
EcoRI G'AATT_C
Cuts at: 3541 3625 4003 3541
Size: 84 378 5854
Fragments arranged by size:
5854 378 84
EcoRV GAT'ATC
Cuts at: 2665 2844 2665
Size: 179 6137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2909 2909
Size: 6316
FspI TGC'GCA
Cuts at: 4822 4822
Size: 6316
HaeII r_GCGC'y
Cuts at: 1678 2045 2137 2764 5463 1678
Size: 367 92 627 2699 2531
Fragments arranged by size:
2699 2531 627 367 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3291 3323 4957 4989 5031
Size: 32 135 32 1719 32 1634 32 42
Cuts at: 5031 5063 1373
Size: 32 2626
Fragments arranged by size:
2626 1719 1634 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3660 3906 669
Size: 988 281 1722 246 3079
Fragments arranged by size:
3079 1722 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6316
HpaI GTT'AAC
Cuts at: 3906 3906
Size: 6316
KasI G'GCGC_C
Cuts at: 2041 2041
Size: 6316
KpnI G_GTAC'C
Cuts at: 3641 3641
Size: 6316
MfeI C'AATT_G
Cuts at: 3915 3915
Size: 6316
MluI A'CGCG_T
Cuts at: 6192 6192
Size: 6316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3121 3408 3444 3552 5430 5614 1305
Size: 1816 287 36 108 1878 184 2007
Fragments arranged by size:
2007 1878 1816 287 184 108 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2422 2948 10
Size: 54 1425 343 590 526 3378
Fragments arranged by size:
3378 1425 590 526 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2156 2477 2582 3026 4274 4633 4792
Size: 648 990 321 105 444 1248 359 159
Cuts at: 4792 518
Size: 2042
Fragments arranged by size:
2042 1248 990 648 444 359 321 159
105
MspA1I CmG'CkG
Cuts at: 1606 2239 2284 3732 4409 5557 5802 1606
Size: 633 45 1448 677 1148 245 2120
Fragments arranged by size:
2120 1448 1148 677 633 245 45
NaeI GCC'GGC
Cuts at: 2103 2103
Size: 6316
NarI GG'CG_CC
Cuts at: 2042 2042
Size: 6316
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4825
Fragments arranged by size:
4825 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6316
NgoMIV G'CCGG_C
Cuts at: 2101 2101
Size: 6316
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6316
NotI GC'GGCC_GC
Cuts at: 3763 3763
Size: 6316
NspI r_CATG'y
Cuts at: 1132 1229 2365 2476 3484 3674 5219 1132
Size: 97 1136 111 1008 190 1545 2229
Fragments arranged by size:
2229 1545 1136 1008 190 111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6316
PciI A'CATG_T
Cuts at: 2472 5215 2472
Size: 2743 3573
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2961 1108
Size: 902 951 4463
Fragments arranged by size:
4463 951 902
PmeI GTTT'AAAC
Cuts at: 3620 3620
Size: 6316
PmlI CAC'GTG
Cuts at: 3513 3513
Size: 6316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2862 2894 4385 4417 5898 5930 2862
Size: 32 1491 32 1481 32 3248
Fragments arranged by size:
3248 1491 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6316
PsiI TTA'TAA
Cuts at: 3886 3886
Size: 6316
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2159 2191 2159
Size: 32 6284
PstI C_TGCA'G
Cuts at: 830 2082 2220 2238 2289 2441 3229 3412 3668
Size: 1252 138 18 51 152 788 183 256
Cuts at: 3668 830
Size: 3478
Fragments arranged by size:
3478 1252 788 256 183 152 138 51
18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6316
PvuII CAG'CTG
Cuts at: 2239 2284 2239
Size: 45 6271
SalI G'TCGA_C
Cuts at: 1936 3658 1936
Size: 1722 4594
SapI GCTCTTCn'nnn_
Cuts at: 2121 2121
Size: 6316
SbfI CC_TGCA'GG
Cuts at: 3668 3668
Size: 6316
ScaI AGT'ACT
Cuts at: 2718 3330 4564 6276 2718
Size: 612 1234 1712 2758
Fragments arranged by size:
2758 1712 1234 612
SexAI A'CCwGG_T
Cuts at: 2199 2199
Size: 6316
SfcI C'TryA_G
Cuts at: 826 1045 2078 2216 2234 2285 2437 2826 3225
Size: 219 1033 138 18 51 152 389 399
Cuts at: 3225 3408 3664 4799 5480 5671 826
Size: 183 256 1135 681 191 1471
Fragments arranged by size:
1471 1135 1033 681 399 389 256 219
191 183 152 138 51 18
SfoI GGC'GCC
Cuts at: 2043 2043
Size: 6316
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6316
SmaI CCC'GGG
Cuts at: 1872 2037 2047 3643 1872
Size: 165 10 1596 4545
Fragments arranged by size:
4545 1596 165 10
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2609 2894 4420 5321 5583 820
Size: 197 926 666 285 1526 901 262 1553
Fragments arranged by size:
1553 1526 926 901 666 285 262 197
SnaBI TAC'GTA
Cuts at: 493 4107 493
Size: 3614 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6316
SphI G_CATG'C
Cuts at: 1132 3674 1132
Size: 2542 3774
SspI AAT'ATT
Cuts at: 5 52 2794 4240 5
Size: 47 2742 1446 2081
Fragments arranged by size:
2742 2081 1446 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6316
StyI C'CwwG_G
Cuts at: 513 1065 2004 2649 3755 513
Size: 552 939 645 1106 3074
Fragments arranged by size:
3074 1106 939 645 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2572 3243 3571 4343 4360 4513
Size: 1255 465 527 671 328 772 17 153
Cuts at: 4513 4698 5143 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 772 671 527 465 445 328
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 2716 2931
Size: 274 80 33 51 1443 597 142 215
Cuts at: 2931 3328 3333 4562 6274 96
Size: 397 5 1229 1712 138
Fragments arranged by size:
1712 1443 1229 597 397 274 215 142
138 80 51 33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2244 2276 2833 2865 6276 6308 2244
Size: 32 557 32 3411 32 2252
Fragments arranged by size:
3411 2252 557 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6316
XbaI T'CTAG_A
Cuts at: 3652 3652
Size: 6316
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6316
XmaI C'CCGG_G
Cuts at: 1870 2035 2045 3641 1870
Size: 165 10 1596 4545
Fragments arranged by size:
4545 1596 165 10
XmnI GAAnn'nnTTC
Cuts at: 2563 2874 4445 2563
Size: 311 1571 4434
Fragments arranged by size:
4434 1571 311
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4124 276
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoO109I FseI FspAI
NruI NsiI PacI PfoI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SfiI SrfI SwaI XcmI