Restriction map for
pFN21AE1968
(Circular) (Six-base) MAPSORT of: pFN21AE1968.seq Check: 8342 from: 1 to: 6298
pFN21AE1968 6298 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3479 3479
Size: 6298
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3287 4108 278
Size: 53 83 186 936 1751 821 2468
Fragments arranged by size:
2468 1751 936 821 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3078 3641 1937
Size: 1141 563 4594
Fragments arranged by size:
4594 1141 563
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2205 2541 4361 5745 6013 1632
Size: 573 336 1820 1384 268 1917
Fragments arranged by size:
1917 1820 1384 573 336 268
AflII C'TTAA_G
Cuts at: 820 1017 2297 820
Size: 197 1280 4821
Fragments arranged by size:
4821 1280 197
AflIII A'CryG_T
Cuts at: 1203 5197 6174 1203
Size: 3994 977 1327
Fragments arranged by size:
3994 1327 977
AgeI A'CCGG_T
Cuts at: 2425 5129 2425
Size: 2704 3594
AhdI GACnn_n'nnGTC
Cuts at: 5027 5027
Size: 6298
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6298
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6298
AlwNI CAG_nnn'CTG
Cuts at: 2607 3209 4041 5613 2607
Size: 602 832 1572 3292
Fragments arranged by size:
3292 1572 832 602
ApaI G_GGCC'C
Cuts at: 3422 3422
Size: 6298
ApaLI G'TGCA_C
Cuts at: 3346 4355 5511 3346
Size: 1009 1156 4133
Fragments arranged by size:
4133 1156 1009
ApoI r'AATT_y
Cuts at: 1493 2258 2284 2323 2805 3302 3607 3833 3985
Size: 765 26 39 482 497 305 226 152
Cuts at: 3985 6094 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 765 497 482 305 226 152
39 26
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 3623 1870
Size: 73 1680 4545
Fragments arranged by size:
4545 1680 73
BamHI G'GATC_C
Cuts at: 3628 3995 3628
Size: 367 5931
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3619 5074 618
Size: 325 217 627 1832 1455 1842
Fragments arranged by size:
1842 1832 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3422 3617 1391
Size: 2031 195 4072
Fragments arranged by size:
4072 2031 195
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3239 928
Size: 971 1340 3987
Fragments arranged by size:
3987 1340 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2261 2682 4190 5400 2261
Size: 421 1508 1210 3159
Fragments arranged by size:
3159 1508 1210 421
BclI T'GATC_A
Cuts at: 1548 2857 1548
Size: 1309 4989
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2531 4909 136
Size: 107 122 71 631 605 859 2378 1525
Fragments arranged by size:
2378 1525 859 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2122 2413 6293 2122
Size: 291 3880 2127
Fragments arranged by size:
3880 2127 291
BlpI GC'TnA_GC
Cuts at: 3715 3715
Size: 6298
Bme1580I G_kGCm'C
Cuts at: 1790 2512 3350 3422 4359 5515 1790
Size: 722 838 72 937 1156 2573
Fragments arranged by size:
2573 1156 937 838 722 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2333 4029 4987
Size: 416 53 83 399 24 916 1696 958
Cuts at: 4987 442
Size: 1753
Fragments arranged by size:
1753 1696 958 916 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 3578 4958 1239
Size: 1385 954 1380 2579
Fragments arranged by size:
2579 1385 1380 954
Bpu10I CC'TnA_GC
Cuts at: 2574 2574
Size: 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4423 5288 5586 4423
Size: 865 298 5135
Fragments arranged by size:
5135 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3596 4961 882
Size: 617 2097 1365 2219
Fragments arranged by size:
2219 2097 1365 617
BsaAI yAC'GTr
Cuts at: 493 1204 2543 4089 493
Size: 711 1339 1546 2702
Fragments arranged by size:
2702 1546 1339 711
BsaBI GATnn'nnATC
Cuts at: 2121 6292 2121
Size: 4171 2127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3284 4105 4487 5122
Size: 53 83 186 936 1751 821 382 635
Cuts at: 5122 275
Size: 1451
Fragments arranged by size:
1751 1451 936 821 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2425 4731 5129 5403 5550 5961 2425
Size: 2306 398 274 147 411 2762
Fragments arranged by size:
2762 2306 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3060 3090 1178
Size: 30 165 30 1657 30 4386
Fragments arranged by size:
4386 1657 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 5412
BseYI C'CCAG_C
Cuts at: 2831 5501 5997 2831
Size: 2670 496 3132
Fragments arranged by size:
3132 2670 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5725
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 3748 4509 5537 1540
Size: 275 186 37 1710 761 1028 2301
Fragments arranged by size:
2301 1710 1028 761 275 186 37
BsiHKAI G_wGCw'C
Cuts at: 2012 2542 3350 3431 4359 4444 5515 2012
Size: 530 808 81 928 85 1071 2795
Fragments arranged by size:
2795 1071 928 808 530 85 81
BsmI GAATG_Cn'
Cuts at: 3809 3902 3809
Size: 93 6205
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2512 2542 3350 3422 3431 3617
Size: 399 222 500 30 808 72 9 186
Cuts at: 3617 4359 4444 5515 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 808 742 500 399 222 186
85 72 30 9
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 4187 5917 4187
Size: 1730 4568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2615 3479 3653 844
Size: 1229 542 864 174 3489
Fragments arranged by size:
3489 1229 864 542 174
BsrBI CCG'CTC
Cuts at: 1394 1823 4185 1394
Size: 429 2362 3507
Fragments arranged by size:
3507 2362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2628 2889 4793 4967 65
Size: 1183 1380 261 1904 174 1396
Fragments arranged by size:
1904 1396 1380 1183 261 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2425 4942 5129 1700
Size: 110 220 395 2517 187 2869
Fragments arranged by size:
2869 2517 395 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 4358 5370 4358
Size: 1012 5286
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2516 794
Size: 1722 4576
BstBI TT'CG_AA
Cuts at: 5102 5102
Size: 6298
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6298
BstXI CCAn_nnnn'nTGG
Cuts at: 2346 2346
Size: 6298
BstYI r'GATC_y
Cuts at: 1972 2122 2413 2548 3226 3535 3628 3995 4380
Size: 150 291 135 678 309 93 367 385
Cuts at: 4380 4397 5849 5935 5947 6293 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 678 385 367 346 309 291
150 135 93 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2131 513
Size: 552 1066 4680
Fragments arranged by size:
4680 1066 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5559
Fragments arranged by size:
5559 683 56
BtsI GCAGTG_nn'
Cuts at: 3117 3814 4608 4628 3117
Size: 697 794 20 4787
Fragments arranged by size:
4787 794 697 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5305 809
Size: 507 3989 1802
Fragments arranged by size:
3989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2532 3745
Size: 54 1425 50 275 18 205 497 1213
Cuts at: 3745 4634 6263 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1213 889 497 275 205 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 3745 1537
Size: 275 223 1710 4090
Fragments arranged by size:
4090 1710 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 4228 1353
Size: 999 1876 3423
Fragments arranged by size:
3423 1876 999
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4885 5259 5405 387
Size: 1064 138 3296 374 146 1280
Fragments arranged by size:
3296 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2205 2541 2624 3578 4361 4958 5745
Size: 393 573 336 83 954 783 597 787
Cuts at: 5745 6013 1239
Size: 268 1524
Fragments arranged by size:
1524 954 787 783 597 573 393 336
268 83
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 2642
Size: 6298
EcoO109I rG'GnC_Cy
Cuts at: 2062 3419 2062
Size: 1357 4941
EcoRI G'AATT_C
Cuts at: 2323 2805 3607 3985 2323
Size: 482 802 378 4636
Fragments arranged by size:
4636 802 482 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2405 2972 2405
Size: 567 5731
FspI TGC'GCA
Cuts at: 4804 4804
Size: 6298
HaeII r_GCGC'y
Cuts at: 1678 2028 2046 5445 1678
Size: 350 18 3399 2531
Fragments arranged by size:
3399 2531 350 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3060 3092 4939 4971 5013
Size: 32 135 32 1488 32 1847 32 42
Cuts at: 5013 5045 1373
Size: 32 2626
Fragments arranged by size:
2626 1847 1488 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3642 3888 669
Size: 988 281 1704 246 3079
Fragments arranged by size:
3079 1704 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6298
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3354 3897 3354
Size: 543 5755
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 5412 5596 1305
Size: 753 3354 184 2007
Fragments arranged by size:
3354 2007 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 10
Size: 54 1425 343 702 3774
Fragments arranged by size:
3774 1425 702 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 2595 3343 4256 4615 4774 518
Size: 648 1081 348 748 913 359 159 2042
Fragments arranged by size:
2042 1081 913 748 648 359 348 159
MspA1I CmG'CkG
Cuts at: 1606 2085 3714 4391 5539 5784 1606
Size: 479 1629 677 1148 245 2120
Fragments arranged by size:
2120 1629 1148 677 479 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5746
NdeI CA'TA_TG
Cuts at: 387 2496 387
Size: 2109 4189
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NspI r_CATG'y
Cuts at: 1132 1229 3134 3656 5201 1132
Size: 97 1905 522 1545 2229
Fragments arranged by size:
2229 1905 1545 522 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6298
PciI A'CATG_T
Cuts at: 5197 5197
Size: 6298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2884 1108
Size: 1776 4522
PfoI T'CCnGG_A
Cuts at: 2640 2640
Size: 6298
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PmlI CAC'GTG
Cuts at: 2543 2543
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2062
Size: 6298
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6298
PsiI TTA'TAA
Cuts at: 2894 3868 2894
Size: 974 5324
PspOMI G'GGCC_C
Cuts at: 3418 3418
Size: 6298
PstI C_TGCA'G
Cuts at: 830 3650 830
Size: 2820 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
SalI G'TCGA_C
Cuts at: 1936 3640 1936
Size: 1704 4594
SbfI CC_TGCA'GG
Cuts at: 3650 3650
Size: 6298
ScaI AGT'ACT
Cuts at: 4546 6258 4546
Size: 1712 4586
SfcI C'TryA_G
Cuts at: 826 1045 2307 2961 3646 4781 5462 5653 826
Size: 219 1262 654 685 1135 681 191 1471
Fragments arranged by size:
1471 1262 1135 685 681 654 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 3625 1872
Size: 1753 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2297 4402 5303 5565 820
Size: 197 926 354 2105 901 262 1553
Fragments arranged by size:
2105 1553 926 901 354 262 197
SnaBI TAC'GTA
Cuts at: 493 4089 493
Size: 3596 2702
SpeI A'CTAG_T
Cuts at: 152 2621 152
Size: 2469 3829
SphI G_CATG'C
Cuts at: 1132 3656 1132
Size: 2524 3774
SspI AAT'ATT
Cuts at: 5 52 2227 4222 5
Size: 47 2175 1995 2081
Fragments arranged by size:
2175 2081 1995 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6298
StyI C'CwwG_G
Cuts at: 513 1065 2345 3737 513
Size: 552 1280 1392 3074
Fragments arranged by size:
3074 1392 1280 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2781 3064 4325 4342 4495 4680 5125
Size: 1255 1201 283 1261 17 153 185 445
Cuts at: 5125 325
Size: 1498
Fragments arranged by size:
1498 1261 1255 1201 445 283 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2316 4544 6256
Size: 274 80 33 51 1443 339 2228 1712
Cuts at: 6256 96
Size: 138
Fragments arranged by size:
2228 1712 1443 339 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2777 2809 6258 6290 2777
Size: 32 3449 32 2785
Fragments arranged by size:
3449 2785 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6298
XbaI T'CTAG_A
Cuts at: 3634 3634
Size: 6298
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 3623 1870
Size: 1753 4545
XmnI GAAnn'nnTTC
Cuts at: 2949 4427 2949
Size: 1478 4820
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3285 4106 276
Size: 53 83 186 936 1751 821 2468
Fragments arranged by size:
2468 1751 936 821 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI