Restriction map for
pFN21AE1967
(Circular) (Six-base) MAPSORT of: pFN21AE1967.seq Check: 7520 from: 1 to: 6295
pFN21AE1967 6295 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3638 1937
Size: 1701 4594
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4358 5742 6010 1632
Size: 2726 1384 268 1917
Fragments arranged by size:
2726 1917 1384 268
AfeI AGC'GCT
Cuts at: 2668 2668
Size: 6295
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6098
AflIII A'CryG_T
Cuts at: 1203 2508 5194 6171 1203
Size: 1305 2686 977 1327
Fragments arranged by size:
2686 1327 1305 977
AgeI A'CCGG_T
Cuts at: 5126 5126
Size: 6295
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3590 1184
Size: 2406 3889
AlwNI CAG_nnn'CTG
Cuts at: 2866 4038 5610 2866
Size: 1172 1572 3551
Fragments arranged by size:
3551 1572 1172
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 3604 3830 3982 6091 1493
Size: 2111 226 152 2109 1697
Fragments arranged by size:
2111 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 2466 2567 3388 3620 1870
Size: 73 92 431 101 821 232 4545
Fragments arranged by size:
4545 821 431 232 101 92 73
BamHI G'GATC_C
Cuts at: 3027 3625 3992 3027
Size: 598 367 5330
Fragments arranged by size:
5330 598 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2109 2282 2316 2471 2947
Size: 325 217 627 322 173 34 155 476
Cuts at: 2947 3134 3616 5071 618
Size: 187 482 1455 1842
Fragments arranged by size:
1842 1455 627 482 476 325 322 217
187 173 155 34
BanII G_rGCy'C
Cuts at: 1391 2029 3094 3350 3395 3614 1391
Size: 638 1065 256 45 219 4072
Fragments arranged by size:
4072 1065 638 256 219 45
BbeI G_GCGC'C
Cuts at: 2113 2320 2951 3138 2113
Size: 207 631 187 5270
Fragments arranged by size:
5270 631 207 187
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2686 2920 928
Size: 971 787 234 4303
Fragments arranged by size:
4303 971 787 234
BbvCI CC'TCA_GC
Cuts at: 3563 3563
Size: 6295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2797 2831 4486 4520 1903
Size: 34 860 34 1655 34 3678
Fragments arranged by size:
3678 1655 860 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2628 4187 5397 2628
Size: 1559 1210 3526
Fragments arranged by size:
3526 1559 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2405 2596 2715
Size: 107 122 71 631 605 733 191 119
Cuts at: 2715 3437 4906 136
Size: 722 1469 1525
Fragments arranged by size:
1525 1469 733 722 631 605 191 122
119 107 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 2663 3712 2663
Size: 1049 5246
Bme1580I G_kGCm'C
Cuts at: 1790 2089 2474 2855 4356 5512 1790
Size: 299 385 381 1501 1156 2573
Fragments arranged by size:
2573 1501 1156 385 381 299
BmgBI CAC'GTC
Cuts at: 1318 2935 1318
Size: 1617 4678
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4026 4984 442
Size: 416 53 83 399 24 2609 958 1753
Fragments arranged by size:
2609 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2436 4955 1239
Size: 803 394 2519 2579
Fragments arranged by size:
2579 2519 803 394
Bpu10I CC'TnA_GC
Cuts at: 3563 3563
Size: 6295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2963 4420 5285 5583 2963
Size: 1457 865 298 3675
Fragments arranged by size:
3675 1457 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2955 3075 3288 4958 882
Size: 617 1456 120 213 1670 2219
Fragments arranged by size:
2219 1670 1456 617 213 120
BsaAI yAC'GTr
Cuts at: 493 1204 2509 2520 4086 493
Size: 711 1305 11 1566 2702
Fragments arranged by size:
2702 1566 1305 711 11
BsaBI GATnn'nnATC
Cuts at: 6289 6289
Size: 6295
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2110 2119 2317 2481
Size: 53 83 186 936 577 9 198 164
Cuts at: 2481 2628 2948 3135 4102 4484 5119 275
Size: 147 320 187 967 382 635 1451
Fragments arranged by size:
1451 967 936 635 577 382 320 198
187 186 164 147 83 53 9
BsaWI w'CCGG_w
Cuts at: 4728 5126 5400 5547 5958 4728
Size: 398 274 147 411 5065
Fragments arranged by size:
5065 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3285 3315 1178
Size: 30 165 30 1882 30 4158
Fragments arranged by size:
4158 1882 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2894 2897 3385 3388 3506 3563 1186
Size: 1708 3 488 3 118 57 3918
Fragments arranged by size:
3918 1708 488 118 57 3 3
BseYI C'CCAG_C
Cuts at: 2278 2310 2743 5498 5994 2278
Size: 32 433 2755 496 2579
Fragments arranged by size:
2755 2579 496 433 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3405 1163
Size: 573 1669 4053
Fragments arranged by size:
4053 1669 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2337 2836 3745 4506 5534 1540
Size: 275 186 336 499 909 761 1028 2301
Fragments arranged by size:
2301 1028 909 761 499 336 275 186
BsiHKAI G_wGCw'C
Cuts at: 2029 2208 2353 2783 3094 4356 4441 5512 2029
Size: 179 145 430 311 1262 85 1071 2812
Fragments arranged by size:
2812 1262 1071 430 311 179 145 85
BsmI GAATG_Cn'
Cuts at: 3806 3899 3806
Size: 93 6202
BsmBI CGTCTCn'nnnn_
Cuts at: 3596 3596
Size: 6295
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2089 2208 2353 2474 2783 2855
Size: 399 239 60 119 145 121 309 72
Cuts at: 2855 3094 3350 3395 3614 4356 4441 5512 1391
Size: 239 256 45 219 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 309 256 239 239
219 145 121 119 85 72 60 45
BspEI T'CCGG_A
Cuts at: 5958 5958
Size: 6295
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3650 844
Size: 2806 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3033 3532 4182 1394
Size: 429 1210 499 650 3507
Fragments arranged by size:
3507 1210 650 499 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2464 3433 4790 4964 65
Size: 1183 1216 969 1357 174 1396
Fragments arranged by size:
1396 1357 1216 1183 969 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4939 5126 1700
Size: 110 3129 187 2869
Fragments arranged by size:
3129 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6295
BssHII G'CGCG_C
Cuts at: 1925 2040 1925
Size: 115 6180
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2850 3428 794
Size: 2056 578 3661
Fragments arranged by size:
3661 2056 578
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstXI CCAn_nnnn'nTGG
Cuts at: 2773 3131 2773
Size: 358 5937
BstYI r'GATC_y
Cuts at: 1972 3027 3625 3992 4377 4394 5846 5932 5944
Size: 1055 598 367 385 17 1452 86 12
Cuts at: 5944 6290 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1055 598 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3546 3546
Size: 6295
BtgI C'CryG_G
Cuts at: 513 1065 2421 2712 3040 3117 3510 513
Size: 552 1356 291 328 77 393 3298
Fragments arranged by size:
3298 1356 552 393 328 291 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 2105 2192 2264 2951 3811 4605 4625 2105
Size: 87 72 687 860 794 20 3775
Fragments arranged by size:
3775 860 794 687 87 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6295
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DraIII CAC_nnn'GTG
Cuts at: 2520 2995 2520
Size: 475 5820
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2690 2939 2981 5302 809
Size: 507 1374 249 42 2321 1802
Fragments arranged by size:
2321 1802 1374 507 249 42
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2334 3513 3742
Size: 54 1425 50 275 18 504 1179 229
Cuts at: 3742 4631 6260 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1179 889 504 275 229 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2334 3742 1537
Size: 275 522 1408 4090
Fragments arranged by size:
4090 1408 522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4225 1353
Size: 2872 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3469 4882 5256 5402 387
Size: 1064 138 1880 1413 374 146 1280
Fragments arranged by size:
1880 1413 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2027 3092 2027
Size: 1065 5230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2436 4358 4955 5742 6010 1239
Size: 393 410 394 1922 597 787 268 1524
Fragments arranged by size:
1922 1524 787 597 410 394 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3283 3283
Size: 6295
EcoRI G'AATT_C
Cuts at: 3604 3982 3604
Size: 378 5917
FspI TGC'GCA
Cuts at: 3074 4801 3074
Size: 1727 4568
HaeII r_GCGC'y
Cuts at: 1678 2113 2171 2320 2527 2670 2866 2951 3138
Size: 435 58 149 207 143 196 85 187
Cuts at: 3138 3186 5442 1678
Size: 48 2256 2531
Fragments arranged by size:
2531 2256 435 207 196 187 149 143
85 58 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3285 3317 4936 4968 5010
Size: 32 135 32 1713 32 1619 32 42
Cuts at: 5010 5042 1373
Size: 32 2626
Fragments arranged by size:
2626 1713 1619 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3538 3639 3885 669
Size: 988 281 1600 101 246 3079
Fragments arranged by size:
3079 1600 988 281 246 101
HindIII A'AGCT_T
Cuts at: 748 3128 748
Size: 2380 3915
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KasI G'GCGC_C
Cuts at: 2109 2316 2947 3134 2109
Size: 207 631 187 5270
Fragments arranged by size:
5270 631 207 187
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 3894 3894
Size: 6295
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5409 5593 1305
Size: 4104 184 2007
Fragments arranged by size:
4104 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4473
Fragments arranged by size:
4473 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4253 4612 4771 518
Size: 648 3087 359 159 2042
Fragments arranged by size:
3087 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2124 2251 2278 2291 2310 2393 2423 2500
Size: 518 127 27 13 19 83 30 77
Cuts at: 2500 2679 2714 2721 3119 3711 4388 5536 5781
Size: 179 35 7 398 592 677 1148 245
Cuts at: 5781 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 592 518 398 245 179
127 83 77 35 30 27 19 13
7
NarI GG'CG_CC
Cuts at: 2110 2317 2948 3135 2110
Size: 207 631 187 5270
Fragments arranged by size:
5270 631 207 187
NcoI C'CATG_G
Cuts at: 513 1065 3510 513
Size: 552 2445 3298
Fragments arranged by size:
3298 2445 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 2603 2662 3103 3653 5198 1132
Size: 97 1374 59 441 550 1545 2229
Fragments arranged by size:
2229 1545 1374 550 441 97 59
PasI CC'CwG_GG
Cuts at: 1348 2321 1348
Size: 973 5322
PciI A'CATG_T
Cuts at: 5194 5194
Size: 6295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2459 3178 3265 3450 3511 1108
Size: 1351 719 87 185 61 3892
Fragments arranged by size:
3892 1351 719 185 87 61
PfoI T'CCnGG_A
Cuts at: 2223 2223
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PmlI CAC'GTG
Cuts at: 2520 2520
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3283 3283
Size: 6295
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 3865 3865
Size: 6295
PstI C_TGCA'G
Cuts at: 830 2074 2256 2419 2643 3225 3237 3255 3647
Size: 1244 182 163 224 582 12 18 392
Cuts at: 3647 830
Size: 3478
Fragments arranged by size:
3478 1244 582 392 224 182 163 18
12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 2310 2500 2310
Size: 190 6105
RsrII CG'GwC_CG
Cuts at: 3456 3456
Size: 6295
SacI G_AGCT'C
Cuts at: 2029 3094 2029
Size: 1065 5230
SacII CC_GC'GG
Cuts at: 2424 2715 3120 2424
Size: 291 405 5599
Fragments arranged by size:
5599 405 291
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 2643 3647 2643
Size: 1004 5291
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SexAI A'CCwGG_T
Cuts at: 3264 3285 3264
Size: 21 6274
SfcI C'TryA_G
Cuts at: 826 1045 2070 2252 2415 2639 3062 3221 3233
Size: 219 1025 182 163 224 423 159 12
Cuts at: 3233 3251 3643 4778 5459 5650 826
Size: 18 392 1135 681 191 1471
Fragments arranged by size:
1471 1135 1025 681 423 392 224 219
191 182 163 159 18 12
SfoI GGC'GCC
Cuts at: 2111 2318 2949 3136 2111
Size: 207 631 187 5270
Fragments arranged by size:
5270 631 207 187
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 2037 3622 1872
Size: 165 1585 4545
Fragments arranged by size:
4545 1585 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2978 4399 5300 5562 820
Size: 197 926 1035 1421 901 262 1553
Fragments arranged by size:
1553 1421 1035 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 2905 152
Size: 2753 3542
SphI G_CATG'C
Cuts at: 1132 3103 3653 1132
Size: 1971 550 3774
Fragments arranged by size:
3774 1971 550
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6295
StyI C'CwwG_G
Cuts at: 513 1065 3510 3734 513
Size: 552 2445 224 3074
Fragments arranged by size:
3074 2445 552 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 4322 4339 4492 4677 5122 325
Size: 1255 690 2052 17 153 185 445 1498
Fragments arranged by size:
2052 1498 1255 690 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4541 6253 96
Size: 274 80 33 51 1443 2564 1712 138
Fragments arranged by size:
2564 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2136 2168 2219 2251 3327 3359 6255 6287 2136
Size: 32 51 32 1076 32 2896 32 2144
Fragments arranged by size:
2896 2144 1076 51 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3299 1316
Size: 1983 4312
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2121 2770 3409 3517 2121
Size: 649 639 108 4899
Fragments arranged by size:
4899 649 639 108
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6295
XmaI C'CCGG_G
Cuts at: 1870 2035 3620 1870
Size: 165 1585 4545
Fragments arranged by size:
4545 1585 165
XmnI GAAnn'nnTTC
Cuts at: 2153 4424 2153
Size: 2271 4024
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI SanDI
SapI SfiI SrfI SwaI