Restriction map for
pFN21AE1962
(Circular) (Six-base) MAPSORT of: pFN21AE1962.seq Check: 6118 from: 1 to: 6286
pFN21AE1962 6286 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3629 1937
Size: 1692 4594
Acc65I G'GTAC_C
Cuts at: 3607 3607
Size: 6286
AclI AA'CG_TT
Cuts at: 1563 3321 4413 4786 1563
Size: 1758 1092 373 3063
Fragments arranged by size:
3063 1758 1092 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2552 3041 3425 4349 5733 6001 1632
Size: 920 489 384 924 1384 268 1917
Fragments arranged by size:
1917 1384 924 920 489 384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 5185 6162 1203
Size: 3982 977 1327
Fragments arranged by size:
3982 1327 977
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 3539 1166
Size: 2373 3913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2713 1184
Size: 1529 4757
AlwNI CAG_nnn'CTG
Cuts at: 4029 5601 4029
Size: 1572 4714
ApaI G_GGCC'C
Cuts at: 2142 2151 2142
Size: 9 6277
ApaLI G'TGCA_C
Cuts at: 4343 5499 4343
Size: 1156 5130
ApoI r'AATT_y
Cuts at: 1493 2208 2408 2702 3417 3595 3821 3973 6082
Size: 715 200 294 715 178 226 152 2109
Cuts at: 6082 1493
Size: 1697
Fragments arranged by size:
2109 1697 715 715 294 226 200 178
152
AseI AT'TA_AT
Cuts at: 160 1032 4840 160
Size: 872 3808 1606
Fragments arranged by size:
3808 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 3147 3611 1870
Size: 73 1204 464 4545
Fragments arranged by size:
4545 1204 464 73
BamHI G'GATC_C
Cuts at: 2020 2473 2536 3616 3983 2020
Size: 453 63 1080 367 4323
Fragments arranged by size:
4323 1080 453 367 63
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3607 5062 618
Size: 325 217 627 1820 1455 1842
Fragments arranged by size:
1842 1820 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2142 2151 3605 1391
Size: 751 9 1454 4072
Fragments arranged by size:
4072 1454 751 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2327 928
Size: 971 428 4887
Fragments arranged by size:
4887 971 428
BbvCI CC'TCA_GC
Cuts at: 2985 2985
Size: 6286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3166 3200 4477 4511 1903
Size: 34 1229 34 1277 34 3678
Fragments arranged by size:
3678 1277 1229 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2146 4897 136
Size: 107 122 71 631 605 474 2751 1525
Fragments arranged by size:
2751 1525 631 605 474 122 107 71
BglII A'GATC_T
Cuts at: 6281 6281
Size: 6286
BlpI GC'TnA_GC
Cuts at: 3703 3703
Size: 6286
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2151 4347 5503 1790
Size: 352 9 2196 1156 2573
Fragments arranged by size:
2573 2196 1156 352 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2099 2440 2933
Size: 416 53 83 399 24 682 341 493
Cuts at: 2933 4017 4975 442
Size: 1084 958 1753
Fragments arranged by size:
1753 1084 958 682 493 416 399 341
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2535 2535
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2112 2376 2581 3281 3303 4946 1239
Size: 873 264 205 700 22 1643 2579
Fragments arranged by size:
2579 1643 873 700 264 205 22
Bpu10I CC'TnA_GC
Cuts at: 2985 2985
Size: 6286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4411 5276 5574 4411
Size: 865 298 5123
Fragments arranged by size:
5123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4949 882
Size: 617 3450 2219
Fragments arranged by size:
3450 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4077 493
Size: 711 2873 2702
Fragments arranged by size:
2873 2702 711
BsaBI GATnn'nnATC
Cuts at: 2628 2650 6280 2628
Size: 22 3630 2634
Fragments arranged by size:
3630 2634 22
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4475 5110 275
Size: 53 83 186 936 2560 382 635 1451
Fragments arranged by size:
2560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2539 2961 3238 3548 4719 5117 5391 5538 5949
Size: 422 277 310 1171 398 274 147 411
Cuts at: 5949 2539
Size: 2876
Fragments arranged by size:
2876 1171 422 411 398 310 277 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2554 2584 1178
Size: 30 165 30 1151 30 4880
Fragments arranged by size:
4880 1151 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6286
BseYI C'CCAG_C
Cuts at: 5489 5985 5489
Size: 496 5790
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3563 1163
Size: 573 1827 3886
Fragments arranged by size:
3886 1827 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3148 3208 3238 3267 4347 4432 5503 3148
Size: 60 30 29 1080 85 1071 3931
Fragments arranged by size:
3931 1080 1071 85 60 30 29
BsmI GAATG_Cn'
Cuts at: 3338 3529 3797 3890 3338
Size: 191 268 93 5734
Fragments arranged by size:
5734 268 191 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2151 3148 3208 3238 3267 3605
Size: 399 352 9 997 60 30 29 338
Cuts at: 3605 4347 4432 5503 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 997 742 399 352 338 85
60 30 29 9
BspEI T'CCGG_A
Cuts at: 2539 3238 3548 5949 2539
Size: 699 310 2401 2876
Fragments arranged by size:
2876 2401 699 310
BspHI T'CATG_A
Cuts at: 2853 4175 5905 2853
Size: 1322 1730 3234
Fragments arranged by size:
3234 1730 1322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3641 844
Size: 2797 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2808 3390 4781 4955 65
Size: 1183 1560 582 1391 174 1396
Fragments arranged by size:
1560 1396 1391 1183 582 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3395 4930 5117 1700
Size: 110 1585 1535 187 2869
Fragments arranged by size:
2869 1585 1535 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 2391 4346 5358 2391
Size: 1955 1012 3319
Fragments arranged by size:
3319 1955 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6286
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 2099 1184
Size: 915 5371
BstXI CCAn_nnnn'nTGG
Cuts at: 2642 3281 2642
Size: 639 5647
BstYI r'GATC_y
Cuts at: 1972 2020 2434 2473 2536 2773 2794 3551 3616
Size: 48 414 39 63 237 21 757 65
Cuts at: 3616 3983 4368 4385 5837 5923 5935 6281 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 757 414 385 367 346 237
86 65 63 48 39 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2143 513
Size: 552 939 139 4656
Fragments arranged by size:
4656 939 552 139
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2314 2615 3080 487
Size: 683 56 1088 301 465 3693
Fragments arranged by size:
3693 1088 683 465 301 56
BtsI GCAGTG_nn'
Cuts at: 2409 3303 3802 4596 4616 2409
Size: 894 499 794 20 4079
Fragments arranged by size:
4079 894 794 499 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3181 3216 529
Size: 35 2617 35 3599
Fragments arranged by size:
3599 2617 35 35
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3153 3397 3733
Size: 54 1425 50 275 18 1323 244 336
Cuts at: 3733 4622 6251 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1323 889 336 275 244 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3495 4216 1353
Size: 2142 721 3423
Fragments arranged by size:
3423 2142 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2376 2552 2581 3041 3281 3303
Size: 393 480 264 176 29 460 240 22
Cuts at: 3303 3425 4349 4946 5733 6001 1239
Size: 122 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 597 480 460 393 268
264 240 176 122 29 22
EcoO109I rG'GnC_Cy
Cuts at: 2138 2139 2148 2244 2386 3104 2138
Size: 1 9 96 142 718 5320
Fragments arranged by size:
5320 718 142 96 9 1
EcoRI G'AATT_C
Cuts at: 2208 3595 3973 2208
Size: 1387 378 4521
Fragments arranged by size:
4521 1387 378
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 5433 1678
Size: 3755 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2554 2586 2848 2880 3361
Size: 32 135 32 982 32 262 32 481
Cuts at: 3361 3393 4927 4959 5001 5033 1373
Size: 32 1534 32 42 32 2626
Fragments arranged by size:
2626 1534 982 481 262 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3630 3876 669
Size: 988 281 1692 246 3079
Fragments arranged by size:
3079 1692 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6286
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3611 3611
Size: 6286
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2422 5400 5584 1305
Size: 1117 2978 184 2007
Fragments arranged by size:
2978 2007 1117 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3155 10
Size: 54 1425 343 1323 3141
Fragments arranged by size:
3141 1425 1323 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2640 3539 4244 4603 4762 518
Size: 648 1474 899 705 359 159 2042
Fragments arranged by size:
2042 1474 899 705 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2059 2372 3702 4379 5527 5772 1606
Size: 453 313 1330 677 1148 245 2120
Fragments arranged by size:
2120 1330 1148 677 453 313 245
NaeI GCC'GGC
Cuts at: 3397 3397
Size: 6286
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4795
Fragments arranged by size:
4795 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NgoMIV G'CCGG_C
Cuts at: 3395 3395
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2231 3022 3187 3644 5189 1132
Size: 97 1002 791 165 457 1545 2229
Fragments arranged by size:
2229 1545 1002 791 457 165 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 5185 5185
Size: 6286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2910 3100 1108
Size: 1802 190 4294
Fragments arranged by size:
4294 1802 190
PfoI T'CCnGG_A
Cuts at: 2790 2831 2790
Size: 41 6245
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 3104 2386
Size: 718 5568
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 2712 3856 2712
Size: 1144 5142
PspOMI G'GGCC_C
Cuts at: 2138 2147 2138
Size: 9 6277
PspXI vC'TCGA_Gb
Cuts at: 3147 3147
Size: 6286
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3122 3154 3122
Size: 32 6254
PstI C_TGCA'G
Cuts at: 830 3638 830
Size: 2808 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 2372 2372
Size: 6286
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SanDI GG'GwC_CC
Cuts at: 2386 3104 2386
Size: 718 5568
SapI GCTCTTCn'nnn_
Cuts at: 3495 3495
Size: 6286
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SfcI C'TryA_G
Cuts at: 826 1045 3634 4769 5450 5641 826
Size: 219 2589 1135 681 191 1471
Fragments arranged by size:
2589 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2146 2146
Size: 6286
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3147 4390 5291 5553 820
Size: 197 926 1204 1243 901 262 1553
Fragments arranged by size:
1553 1243 1204 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 2231 3187 3644 1132
Size: 1099 956 457 3774
Fragments arranged by size:
3774 1099 956 457
SspI AAT'ATT
Cuts at: 5 52 3278 4210 5
Size: 47 3226 932 2081
Fragments arranged by size:
3226 2081 932 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6286
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 2904 3725 513
Size: 552 939 285 615 821 3074
Fragments arranged by size:
3074 939 821 615 552 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2214 4313 4330 4483 4668 5113 325
Size: 1255 634 2099 17 153 185 445 1498
Fragments arranged by size:
2099 1498 1255 634 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3501 4532 6244
Size: 274 80 33 51 1443 1524 1031 1712
Cuts at: 6244 96
Size: 138
Fragments arranged by size:
1712 1524 1443 1031 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6246 6278 6246
Size: 32 6254
Tth111I GACn'n_nGTC
Cuts at: 1316 2546 1316
Size: 1230 5056
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 3147 1943
Size: 1204 5082
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 2877 4415 2877
Size: 1538 4748
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PmlI RsrII SacI SacII SexAI SfoI SrfI SwaI
XcmI